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J Clin Microbiol. 1994 February; 32(2): 452–456.
PMCID: PMC263052

Genotypic variation and slime production among blood and catheter isolates of Candida parapsilosis.


Candida parapsilosis is an important nosocomial pathogen that can proliferate in high concentrations of glucose and form biofilms on prosthetic materials. We investigated the genotypic diversity and slime production among 31 isolates of C. parapsilosis from individual patients with bloodstream or catheter infections. DNA subtyping was performed by using electrophoretic karyotyping plus restriction endonuclease analysis with BssHII followed by pulsed-field gel electrophoresis. Slime production was evaluated by growing organisms in Sabouraud broth with 8% glucose and examining the walls of the tubes for the presence of an adherent slime layer. Overall there were 14 DNA subtypes among the 31 isolates. Eighty percent of the isolates produced slime; 67% of the isolates were moderately to strongly positive, 13% were weakly positive, and 20% were not slime producers. The ability of isolates of a given DNA type to produce slime under these conditions was variable. The results of these studies indicate moderate genotypic variation among clinical isolates of C. parapsilosis. The propensity of these isolates to form slime in glucose-containing solutions suggests that this phenotypic characteristic may contribute to the ability of C. parapsilosis to adhere to plastic catheters and cause infections.

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Selected References

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  • Banerjee SN, Emori TG, Culver DH, Gaynes RP, Jarvis WR, Horan T, Edwards JR, Tolson J, Henderson T, Martone WJ. Secular trends in nosocomial primary bloodstream infections in the United States, 1980-1989. National Nosocomial Infections Surveillance System. Am J Med. 1991 Sep 16;91(3B):86S–89S. [PubMed]
  • Carruba G, Pontieri E, De Bernardis F, Martino P, Cassone A. DNA fingerprinting and electrophoretic karyotype of environmental and clinical isolates of Candida parapsilosis. J Clin Microbiol. 1991 May;29(5):916–922. [PMC free article] [PubMed]
  • Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun. 1982 Jul;37(1):318–326. [PMC free article] [PubMed]
  • Davenport DS, Massanari RM, Pfaller MA, Bale MJ, Streed SA, Hierholzer WJ., Jr Usefulness of a test for slime production as a marker for clinically significant infections with coagulase-negative staphylococci. J Infect Dis. 1986 Feb;153(2):332–339. [PubMed]
  • Diaz-Mitoma F, Harding GK, Hoban DJ, Roberts RS, Low DE. Clinical significance of a test for slime production in ventriculoperitoneal shunt infections caused by coagulase-negative staphylococci. J Infect Dis. 1987 Oct;156(4):555–560. [PubMed]
  • Doebbeling BN, Lehmann PF, Hollis RJ, Wu LC, Widmer AF, Voss A, Pfaller MA. Comparison of pulsed-field gel electrophoresis with isoenzyme profiles as a typing system for Candida tropicalis. Clin Infect Dis. 1993 Mar;16(3):377–383. [PubMed]
  • Franson TR, Sheth NK, Rose HD, Sohnle PG. Scanning electron microscopy of bacteria adherent to intravascular catheters. J Clin Microbiol. 1984 Sep;20(3):500–505. [PMC free article] [PubMed]
  • Herwaldt LA, Boyken LD, Pfaller MA. Biotyping of coagulase-negative staphylococci. 108 isolates from nosocomial bloodstream infections. Diagn Microbiol Infect Dis. 1990 Nov-Dec;13(6):461–466. [PubMed]
  • Ishak MA, Gröschel DH, Mandell GL, Wenzel RP. Association of slime with pathogenicity of coagulase-negative staphylococci causing nosocomial septicemia. J Clin Microbiol. 1985 Dec;22(6):1025–1029. [PMC free article] [PubMed]
  • Komshian SV, Uwaydah AK, Sobel JD, Crane LR. Fungemia caused by Candida species and Torulopsis glabrata in the hospitalized patient: frequency, characteristics, and evaluation of factors influencing outcome. Rev Infect Dis. 1989 May-Jun;11(3):379–390. [PubMed]
  • Lott TJ, Kuykendall RJ, Welbel SF, Pramanik A, Lasker BA. Genomic heterogeneity in the yeast Candida parapsilosis. Curr Genet. 1993 May-Jun;23(5-6):463–467. [PubMed]
  • Marrie TJ, Cooper JH, Costerton JW. Ultrastructure of Candida parapsilosis endocarditis. Infect Immun. 1984 Aug;45(2):390–398. [PMC free article] [PubMed]
  • Marrie TJ, Costerton JW. Scanning and transmission electron microscopy of in situ bacterial colonization of intravenous and intraarterial catheters. J Clin Microbiol. 1984 May;19(5):687–693. [PMC free article] [PubMed]
  • O'Day DM, Head WS, Robinson RD. An outbreak of Candida parapsilosis endophthalmitis: analysis of strains by enzyme profile and antifungal susceptibility. Br J Ophthalmol. 1987 Feb;71(2):126–129. [PMC free article] [PubMed]
  • Painter BG, Isenberg HD. Isolation of Candida parapsilosis: report of two cases. Am J Clin Pathol. 1973 Jan;59(1):62–65. [PubMed]
  • Pfaller M, Wenzel R. Impact of the changing epidemiology of fungal infections in the 1990s. Eur J Clin Microbiol Infect Dis. 1992 Apr;11(4):287–291. [PubMed]
  • Plouffe JF, Brown DG, Silva J, Jr, Eck T, Stricof RL, Fekety FR., Jr Nosocomial outbreak of Candida parapsilosis fungemia related to intravenous infusions. Arch Intern Med. 1977 Dec;137(12):1686–1689. [PubMed]
  • Solomon SL, Khabbaz RF, Parker RH, Anderson RL, Geraghty MA, Furman RM, Martone WJ. An outbreak of Candida parapsilosis bloodstream infections in patients receiving parenteral nutrition. J Infect Dis. 1984 Jan;149(1):98–102. [PubMed]
  • Vazquez JA, Beckley A, Donabedian S, Sobel JD, Zervos MJ. Comparison of restriction enzyme analysis versus pulsed-field gradient gel electrophoresis as a typing system for Torulopsis glabrata and Candida species other than C. albicans. J Clin Microbiol. 1993 Aug;31(8):2021–2030. [PMC free article] [PubMed]
  • Weems JJ., Jr Candida parapsilosis: epidemiology, pathogenicity, clinical manifestations, and antimicrobial susceptibility. Clin Infect Dis. 1992 Mar;14(3):756–766. [PubMed]
  • Weems JJ, Jr, Chamberland ME, Ward J, Willy M, Padhye AA, Solomon SL. Candida parapsilosis fungemia associated with parenteral nutrition and contaminated blood pressure transducers. J Clin Microbiol. 1987 Jun;25(6):1029–1032. [PMC free article] [PubMed]

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