Search tips
Search criteria 


Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. 1985 August; 49(2): 440–442.
PMCID: PMC262037

Trypsin-sensitive, bovine serum albumin-dependent hemolysis activity in Mycoplasma pulmonis.


Although Mycoplasma pulmonis did not lyse normal erythrocytes, it rapidly lysed erythrocytes that had cytoskeletal deficiencies which allow increased diffusion of membrane glycophorin or that had been treated with trypsin to remove surface proteins. This hemolysis occurred only in the presence of bovine serum albumin and was eliminated by trypsin treatment of the mycoplasma. Hemolytic activity was restored after such trypsin treatment when mycoplasma protein synthesis was allowed. M. pulmonis hemolytic activity was not diffusible and thus differed from the activities reported for other mycoplasmas, which involve small diffusible intermediates such as hydrogen peroxide. With the exception of the requirement for bovine serum albumin, the factors which affected hemolysis were similar to those which we have previously reported to affect M. pulmonis hemagglutination, suggesting that these two activities are functionally related.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (607K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bodine DM, 4th, Birkenmeier CS, Barker JE. Spectrin deficient inherited hemolytic anemias in the mouse: characterization by spectrin synthesis and mRNA activity in reticulocytes. Cell. 1984 Jul;37(3):721–729. [PubMed]
  • Brennan PC, Feinstein RN. Relationship of hydrogen peroxide production by Mycoplasma pulmonis to virulence for catalase-deficient mice. J Bacteriol. 1969 Jun;98(3):1036–1040. [PMC free article] [PubMed]
  • Cassell GH, Wilborn WH, Silvers SH, Minion FC. Adherence and colonization of Mycoplasma pulmonis to genital epithelium and spermatozoa in rats. Isr J Med Sci. 1981 Jul;17(7):593–598. [PubMed]
  • Cole BC, Ward JR, Martin CH. Hemolysin and peroxide activity of Mycoplasma species. J Bacteriol. 1968 Jun;95(6):2022–2030. [PMC free article] [PubMed]
  • Efrati H, Rottem S, Razin S. Lipid and protein membrane components associated with cholesterol uptake by Mycoplasmas. Biochim Biophys Acta. 1981 Mar 6;641(2):386–394. [PubMed]
  • Gul S, Smith AD. Haemolysis of washed human red cells by the combined action of Naja naja phospholipase A 2 and albumin. Biochim Biophys Acta. 1972 Oct 23;288(1):237–240. [PubMed]
  • Gul S, Smith AD. Haemolysis of intact human erythrocytes by purified cobra venom phospholipase A2 in the presence of albumin and Ca2+. Biochim Biophys Acta. 1974 Nov 15;367(3):271–281. [PubMed]
  • Haest CW. Interactions between membrane skeleton proteins and the intrinsic domain of the erythrocyte membrane. Biochim Biophys Acta. 1982 Dec;694(4):331–352. [PubMed]
  • Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed]
  • Koppel DE, Sheetz MP, Schindler M. Matrix control of protein diffusion in biological membranes. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3576–3580. [PubMed]
  • Marchesi VT, Furthmayr H, Tomita M. The red cell membrane. Annu Rev Biochem. 1976;45:667–698. [PubMed]
  • Minion FC, Cassell GH, Pnini S, Kahane I. Multiphasic interactions of Mycoplasma pulmonis with erythrocytes defined by adherence and hemagglutination. Infect Immun. 1984 May;44(2):394–400. [PMC free article] [PubMed]
  • Minion FC, Goguen JD. Diffusion of target cell membrane proteins allows recognition of cryptic binding sites by Mycoplasma pulmonis hemagglutinin. Infect Immun. 1985 May;48(2):579–580. [PMC free article] [PubMed]
  • Razin S. The mycoplasmas. Microbiol Rev. 1978 Jun;42(2):414–470. [PMC free article] [PubMed]
  • Sheetz MP, Schindler M, Koppel DE. Lateral mobility of integral membrane proteins is increased in spherocytic erythrocytes. Nature. 1980 Jun 12;285(5765):510–511. [PubMed]
  • Shohet SB. Reconstitution of spectrin-deficient, spherocytic mouse erythrocyte membranes. J Clin Invest. 1979 Aug;64(2):483–494. [PMC free article] [PubMed]
  • Wise KS, Cassell GH, Action RT. Selective association of murine T lymphoblastoid cell surface alloantigens with Mycoplasma hyorhinis. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4479–4483. [PubMed]
  • Wise KS, Minion FC, Cheung HC. Translocation of Thy-1 antigen and a fluorescent lipid probe during lymphoblastoid cell interaction with Mycoplasma hyorhinis. Rev Infect Dis. 1982 May-Jun;4 (Suppl):S210–S218. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)