PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
 
Infect Immun. 1985 August; 49(2): 291–297.
PMCID: PMC262013

Demonstration of a capsule plasmid in Bacillus anthracis.

Abstract

Virulent and certain avirulent strains of Bacillus anthracis harbor a plasmid, designated pXO2, which is involved in the synthesis of capsules. Two classes of rough, noncapsulated (Cap-) variants were isolated from the capsule-producing (Cap+) Pasteur vaccine strains ATCC 6602 and ATCC 4229. One class was cured of pXO2, and the other class still carried it. Reversion to Cap+ was demonstrable only in rough variants which had retained pXO2. Proof that pXO2 is involved in capsule synthesis came from experiments in which the plasmid was transferred by CP-51-mediated transduction and by a mating system in which plasmid transfer is mediated by a Bacillus thuringiensis fertility plasmid, pXO12. Cells of Bacillus cereus and a previously noncapsulated (pXO2-) strain of B. anthracis produced capsules after the acquisition of pXO2.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Uchida I, Sekizaki T, Hashimoto K, Terakado N. Association of the encapsulation of Bacillus anthracis with a 60 megadalton plasmid. J Gen Microbiol. 1985 Feb;131(2):363–367. [PubMed]
  • Battisti L, Green BD, Thorne CB. Mating system for transfer of plasmids among Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis. J Bacteriol. 1985 May;162(2):543–550. [PMC free article] [PubMed]
  • BEALL FA, TAYLOR MJ, THORNE CB. Rapid lethal effect in rats of a third component found upon fractionating the toxin of Bacillus anthracis. J Bacteriol. 1962 Jun;83:1274–1280. [PMC free article] [PubMed]
  • Kado CI, Liu ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. [PMC free article] [PubMed]
  • MEYNELL EW. REVERTING AND NON-REVERTING ROUGH VARIANTS OF BACILLUS ANTHRACIS. J Gen Microbiol. 1963 Jul;32:55–60. [PubMed]
  • Meynell GG, Meynell E. The biosynthesis of poly d-glutamic acid, the capsular material of Bacillus anthracis. J Gen Microbiol. 1966 Apr;43(1):119–138. [PubMed]
  • Mikesell P, Ivins BE, Ristroph JD, Dreier TM. Evidence for plasmid-mediated toxin production in Bacillus anthracis. Infect Immun. 1983 Jan;39(1):371–376. [PMC free article] [PubMed]
  • Ristroph JD, Ivins BE. Elaboration of Bacillus anthracis antigens in a new, defined culture medium. Infect Immun. 1983 Jan;39(1):483–486. [PMC free article] [PubMed]
  • Ruhfel RE, Robillard NJ, Thorne CB. Interspecies transduction of plasmids among Bacillus anthracis, B. cereus, and B. thuringiensis. J Bacteriol. 1984 Mar;157(3):708–711. [PMC free article] [PubMed]
  • STANLEY JL, SARGEANT K, SMITH H. Purification of factors I and II of the anthrax toxin produced in vivo. J Gen Microbiol. 1960 Feb;22:206–218. [PubMed]
  • THORNE CB. Biochemical properties of virulent and avirulent strains of Bacillus anthracis. Ann N Y Acad Sci. 1960 Nov 21;88:1024–1033. [PubMed]
  • Thorne CB. Transducing bacteriophage for Bacillus cereus. J Virol. 1968 Jul;2(7):657–662. [PMC free article] [PubMed]
  • Thorne CB. Transduction in Bacillus thuringiensis. Appl Environ Microbiol. 1978 Jun;35(6):1109–1115. [PMC free article] [PubMed]
  • THORNE CB, GOMEZ CG, HOUSEWRIGHT RD. Synthesis of glutamic acid and glutamyl polypeptide by Bacillus anthracis. II. The effect of carbon dioxide on peptide production on solid media. J Bacteriol. 1952 Mar;63(3):363–368. [PMC free article] [PubMed]
  • THORNE CB, MOLNAR DM, STRANGE RE. Production of toxin in vitro by Bacillus anthracis and its spearation into two components. J Bacteriol. 1960 Mar;79:450–455. [PMC free article] [PubMed]
  • Vodkin MH, Leppla SH. Cloning of the protective antigen gene of Bacillus anthracis. Cell. 1983 Sep;34(2):693–697. [PubMed]
  • Yelton DB, Thorne CB. Comparison of Bacillus cereus bacteriophages CP-51 and CP-53. J Virol. 1971 Aug;8(2):242–253. [PMC free article] [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)