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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Am Chem Soc. Author manuscript; available in PMC 2008 December 27.
Published in final edited form as:
PMCID: PMC2610334
NIHMSID: NIHMS82036

Strain-Release Rearrangement of N-Vinyl-2-Arylaziridines. Total Synthesis of the Anti-Leukemia Alkaloid (–)-Deoxyharringtonine

The cephalotaxus esters constitute a family of remarkably potent anti-leukemia alkaloids from the Cephalotaxus genus.1 Several of these alkaloids, including deoxyharringtonine (1, Chart 1), exhibit acute toxicity toward P388 and L1210 leukemia cells with IC50 values in the ng/mL range.1 The bulk of the multistep synthetic efforts toward these targets have involved innovative routes to cephalotaxine (2),2 the most abundant constituent of this family of alkaloids. However, 2, itself, is biologically inactive, and the difficulties associated with acylation of its hindered C3-hydroxyl with sterically demanding carboxyl derivatives, such as that in 1, have been noted.1,3 We report the total synthesis of (–)-deoxyharringtonine (1), employing novel synthetic strategies not only for the preparation of the [3]benzazepine and the spiro-fused pyrrolidine substructures present in cephalotaxine (2), but also for hindered acyl chain synthesis and attachment to 2 for efficient access to the biologically relevant and rare cephalotaxus esters.4

Strain-release [3,3]-rearrangements of N-aryl-2-vinylaziridines to generate [1]benzazepines are documented;5 however, to our knowledge, successful [3,3]-rearrangements of N-vinyl-2-arylaziridines to form [3]benzazepines, such as that present in 2, have not been reported. The feasibility of this reaction was assessed in a model investigation (Scheme 1) commencing with the conversion of 3,4-methylenedioxyacetophenone (3) to aziridine 4 via the sequential steps of oxime formation (HONH3Cl, NaOH, 87%) and reductive Neber rearrangement (LiAlH4, HN(CHMe2)2, 88%).6 Treatment of aziridine 4 with 3-chloro-2-cyclopentenone under basic conditions afforded the benzylic chloride 5 (64%), likely the result of conjugate addition–elimination followed by chloride-mediated aziridine opening. The key N-aryl-2-vinylaziridine was regenerated in situ (Cs2CO3, 1,4-dioxane) and underwent sequential thermal rearrangement (100 °C, 67)7 and tautomerization to provide 8 (67%), an achiral version of the tetracyclic fragment within 2.

Scheme 1 a
a Reagents and conditions: (a) HONH3Cl, NaOH, EtOH, H2O, 80 °C, 87%; (b) LiAlH4, HN(CHMe2)2, THF, 60 °C, 88%; (c) 3-chloro-2-cyclopentenone, Et3N, THF, 60 °C, 64%; (d) Cs2CO3, 1,4-dioxane, 100 °C, 67%.

The establishment of this convergent approach to [3]benzazepine construction permitted its application to the synthesis of (–)-cephalotaxine (2, Scheme 2). Introduction of the β-chloro substituent on (S,S)-4,5-dihydroxycyclopent-2-enone isopropylidene acetal (9)8 was accomplished in a four-step sequence involving (1) Luche reduction of the enone to afford the (R)-allylic alcohol, (2) chloroselenenylation of the alkene (73%, two steps), (3) selenide oxidation–elimination to generate the corresponding vinyl chloride (91%), and (4) Dess-Martin periodinane (DMP) oxidation of the allylic alcohol to provide the β-chloroenone 10 (98%). Conjugate addition–elimination of 10 with racemic aziridine 4 provided N-vinyl-2-arylaziridine 11 (85%) as a 1:1 mixture of benzylic diastereomers. Smooth [3,3]-rearrangement of the epimeric aziridines 11 ensued upon thermal activation (100 °C) to provide [3]-benzazepine 12 (76%),9 the nonracemic tetracyclic core of (–)-2.

Scheme 2 a
a Reagents and conditions: (a) NaBH4, CeCl3, MeOH, 23 °C; (b) PhSeCl, CH2Cl2, 23 °C, 73% (2 steps); (c) m-CPBA, CH2Cl2, 23 °C; Et3N, 40 °C, 91%; (d) DMP, THF, 23 °C, 98%; (e) 4, Et3N, THF, 23 °C, 85%; (f) ...

The vinylogous amide group in 12 allowed for application of a variant of our recently disclosed approach to pyrrolidine construction from tertiary vinylogous amide precursors.10 Thus, [3]benzazepine 12 was N-alkylated with Me3SiCH2I (75%) to generate the tertiary vinylogous amide 13, which permitted selective carbonyl O-acylation (pivaloyl triflate) followed by C-desilylation (TBAT)11 to produce the transient nonstabilized azomethine ylide 14. The presence of PhSO2CH=CH2 led to stereo- and regioselective dipolar cycloaddition to form the spiro-pyrrolidine 15 (77%). Notably, X-ray analysis of 15 confirmed that the cycloaddition proceeded via an apparent contra-steric face-selective approach of the dipolarophile onto ylide 14, leading to the required C5 R configuration.12

Further functional group interconversions in the synthesis of (–)-2 involved SmI2-mediated reductive desulfonylation (74%)13 and exchange of the C3 enol ester in 15 to its enol benzyl carbonate counterpart 16 (85%, two steps). Manipulation of the C1–C2 oxidation states in 16 was then accomplished by isopropylidene removal followed by sequential Yb(OTf)3-mediated selective C1-O-acylation (Boc2O)14 and C2 oxidation (IBX) to form the corresponding C2 ketone (50%, two steps). Subsequent C1 deoxygenation (CrCl2) and benzyl carbonate hydrogenolysis provided the enol 17 (42%, two steps), which allowed for its two-step conversion to (–)-(2) via C2 enol ether formation (55%) and stereoselective C3 reduction (95%).2h

Incorporation of preformed acyl chains of bioactive cephalotaxus esters onto 2 has proven to be challenging on two fronts. Typically, protracted synthetic routes to these chiral acyl fragments are required,15 and their direct attachment to the C3–OH of 2 is often inefficient, if not prohibitive.3,16 Thus, a short nonracemic synthesis of the acyl chain of (–)-1 was developed (Scheme 3), commencing with acetal derivatization of d-malic acid (18) with Me3CHO to afford [1,3]dioxolanone 19 (82%).17 The C2′ stereocenter in the acyl chain was established via double deprotonation of 19 followed by diastereoselective C2′ alkylation18 with prenyl bromide to provide the corresponding C2′-R-[1,3]dioxolanone (66%). Transesterification with acetal removal (BnOH) then provided the tertiary alcohol 20 (88%). The hydroxy acid 20 was cyclized via the Yamaguchi anhydride19 to provide the β-lactone, allowing for alkene hydrogenation and benzyl ester hydrogenolysis to yield the carboxylic acid 21 (99%). Activation of 21 with 2,4,6-Cl3C6H2COCl19 proceeded without rupture of the β-lactone, allowing for acylation of 2 to afford ester 22 (81%). Methanolysis of the β-lactone in 22 concluded the synthesis of (–)-deoxyharringtonine (1, 76%).

Scheme 3 a
a Reagents and conditions: (a) TMSCl, TMS2NH, CH2Cl2, 23 °C; Me3CCHO, Me3SiOTf, CH2Cl2, −25 °C, 82%; (b) LHMDS, Me2C=CHCH2Br, THF, −78 °C, 66%; (c) NaH, BnOH, THF, 0 °C; 88%; (d) 2,4,6-Cl3C6H2COCl, DMAP, ...

The relative ease with which cephalotaxine (2) is acylated by the β-lactone 21 highlights this approach for the synthesis of the anti-leukemia cephalotaxus esters. This, in conjunction with novel strategies for N-heterocycle synthesis that include the rearrangement of an N-vinyl-2-arylaziridine and a vinylogous amide acylation–cycloaddition cascade, should allow rapid access to other related structures of potential therapeutic utility.

Supplementary Material

si

Supporting Information Available:

Experimental details (PDF). This material is available free of charge via the Internet at http://pubs.acs.org.

Acknowledgments

This research was supported by the NIH-NIGMS (GM67659), Merck, Pfizer, Eli Lilly, and Abbott. A Procter & Gamble graduate fellowship to J.D.E. is acknowledged.

References

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