In fruit flies, specific odorants interact with unique combinations of olfactory sensory neurons giving rise to a putative topographic odor code of activated glomeruli in the antennal lobe. To test the requirement of differential spatial encoding in odorant discrimination we reduced olfactory input complexity using Or83b2
null mutant flies (13
). OR83b is an essential subunit of odorant receptor (OR) containing odorant-gated cation channels (13
). Most fruit fly OSNs co-express Or83b
with a single unique (OR) gene and all those housed in basiconic and trichoid sensillae, with the exception of a highly specialized class that detect CO2
, require Or83b
for function (13
is also co-expressed with Or35a
in a broadly tuned class of coeloconic OSNs, but the remaining OSNs in coeloconic sensillae, specialized to select volatiles including small amines, have not been reported to express Or83b
). Therefore, Or83b2
mutant flies are anosmic to odorants sensed by basiconic and trichoid sensillae. Importantly, OSNs wire to the appropriate glomeruli in Or83b
mutant flies and one can restore function to a single OSN class by expressing a uas-Or83b
transgene using Or
-specific GAL4 control (20
). Using this technique others demonstrated that larvae with a single OSN chemotax toward odorants that attract wild-type larvae (20
). While clearly establishing a role for single OSNs, these studies did not investigate whether odorant-evoked activity through a single class of OSN can be decoded as a discrete odor percept. One way to do this is to assign value to an arbitrary odorant with associative conditioning and demonstrate that flies choose appropriately between odorants. If discrete spatial patterns of glomerular activation are essential for encoding odorant identity, flies with one OSN class will fail to discriminate odorants, because the glomerulus activated by all odorants is the same in these flies. Odorant discrimination with one class of OSNs would challenge a spatial encoding model.
We used an olfactory conditioning paradigm where flies associate one of two odorants with electric shock punishment and then choose between the two odorants (22
). Trained flies preferentially avoid the T-maze arm with the conditioned odorant. A different population of the same genotype of flies is subsequently taught to associate the other odorant with punishment and a single learning score represents the average of the two reciprocal experiments. This design provides a rigorous test of odorant discrimination and controls against innate odorant bias.
The electrophysiological response to a large panel of odorants has been reported for most Drosophila
), allowing us to select and test OSNs and their cognate odorants. We first determined whether Or83b2
mutant flies can learn to discriminate between six pairs of odorants (6-methyl-5-hepten-2-one versus pentyl acetate, methyl salicylate versus methyl benzoate, isoamyl acetate versus methyl benzoate, methyl hexanoate versus di-ethyl succinate, methyl salicylate versus 4-methyl phenol and geranyl acetate versus ethyl acetate) selected because they activate defined ORs (). As expected, wild-type flies showed robust learned discrimination with all six odorant pairs whereas Or83b2
mutant flies did not. Therefore, Or83b
expressing OSNs are required to learn to discriminate between the chosen odorants and residual responses in Or83b2
mutant flies are not sufficient to support learned odorant discrimination.
Or83b2 flies with functional Or46a, Or67a or Or98a-expressing neurons learn to discriminate between odorants that activate these receptors
We next tested flies in which the function of Or46a
-expressing OSNs were restored individually. These OSNs are housed in different sensory sensilla (pb2, ab10 and ab7a) in the maxillary palp or antenna, project their axons to the spatially discrete VA7l, DM6 and VM5 glomeruli (), and respond to a subset of the odorant pairs used in (6
). Furthermore, these receptors are not co-expressed with other functional ORs (6
). We first used Or46a
-GAL4 to express uas-Or83b
in an otherwise Or83b2
mutant fly and tested whether these flies could discriminate between two odorants reported to activate OR46a; 4-methyl phenol and methyl salicylate (23
). Re-establishing OR46a OSN function in this way faithfully restored odor-evoked responses to those approximating wild-type OR46a neurons (23
). We assayed wild-type, Or83b2
mutant flies in parallel for comparison (). All flies without functional Or83b
-expressing OSNs did not learn whereas flies with restored OR46a neurons learned to discriminate between 4-methyl phenol and methyl salicylate. As an indicator of specificity, we tested flies with restored OR67a neurons. OR67a is broadly tuned but apparently does not respond to 4-methyl phenol and methyl salicylate (11
). Consistent with this, OR67a restored flies did not learn with these odorants (). Therefore flies with a single class of functional Or83b
-expressing OSNs can discriminate between two odorants if they activate the relevant OR.
The odor-tuning curve of OR67a partially overlaps with that of OR98a (11
). We therefore tested flies with restored OR67a or OR98a neurons for learned discrimination between methyl benzoate and isoamyl acetate (). As in the previous experiments, all flies without functional Or83b
-expressing OSNs did not learn but robust learning was evident in flies with restored OR67a or OR98a neurons. Therefore Or83b2
mutant flies can use either OR67a or OR98a-restored OSNs to discriminate between methyl benzoate and isoamyl acetate.
OR67a also responds to pentyl acetate and 6-methyl-5-hepten-2-one (11
). We therefore tested whether flies with restored OR67a neurons could learn to discriminate between these two odorants (). All flies without functional Or83b
-expressing OSNs did not exhibit learning, whereas flies with restored OR67a OSNs learned. Therefore OR67a restored flies can learn to discriminate between at least two pairs of different odorants that activate OR67a. The preceding experiments demonstrate that flies can employ a single class of Or83b
-expressing OSNs to learn to discriminate between two odorants that activate that OSN class, consistent with the notion that they can use neural activity in the same class of OSNs to differentially represent odorants.
Our findings using Or83b2 mutant flies suggest that Or83b-independent OSNs are not sufficient for learned discrimination with multiple odorant combinations (). Nevertheless, we further tested whether flies with restored OR67a neurons had other relevant OSNs by testing whether flies could simultaneously encode multiple odorant components, like wild-type flies. We combined the four odorants that flies with restored OR67a neurons can discriminate between; methyl benzoate, isoamyl acetate, pentyl acetate and 6-methyl-5-hepten-2-one, into two binary mixtures, trained the flies with these mixtures () and tested for discrimination between the component odorants. Whereas wild-type flies exhibited learned discrimination for all four component odorants, regardless of the mixture combination used during training, learned discrimination was only observed for the 6-methyl-5-hepten-2-one and pentyl acetate components in OR67a restored flies. These data suggest that OR67a restored flies encode one odor component at a time, consistent with the notion that these odorants activate the same OSNs.
Limitations in learned behavior in Or83b2 flies with functional Or67a-expressing neurons
To further test the model that odorants compete for ORs in OR67a restored flies, we trained flies with single odorants and tested discrimination with binary mixtures (). We reasoned that a competing odorant in a mixture during testing, would mask learned behavior for the other odorant. Training wild-type flies with either 6-methyl-5-hepten-2-one versus pentyl acetate or isoamyl acetate versus methyl benzoate and testing with 6-methyl-5-hepten-2-one + isoamyl acetate versus pentyl acetate + methyl benzoate revealed learned discrimination in both cases (). However, in flies with OR67a restored neurons, robust learned discrimination was only observed following training with 6-methyl-5-hepten-2-one versus pentyl acetate and not with isoamyl acetate versus methyl benzoate. We also tested learned discrimination with a different odorant combination; 6-methyl-5-hepten-2-one + methyl benzoate versus pentyl acetate + isoamyl acetate (). Wild-type flies showed learned discrimination in both cases, whereas OR67a restored flies only showed learned discrimination when trained with 6-methyl-5-hepten-2-one versus pentyl acetate. Therefore these data are consistent with the notion that odorants compete at the OSN level in OR67a restored flies, providing further support that these flies have a single relevant OSN class (Fig. S1
We also tested the contribution of Or83b-independent OSNs using a cross-adaptation assay that does not require learning. We predicted OR67a restored flies would cross-adapt to odorants that activate OR67a OSNs if these odorants activated the same OSNs. We first used methyl benzoate and pentyl acetate because odorant mixture experiments suggested these odorants compete for OR67a neurons (). Wild-type and OR67a restored flies were adapted by pre-exposure to methyl benzoate for 30 minutes and tested for methyl benzoate or pentyl acetate avoidance behavior. Naive wild-type and OR67a restored flies avoided methyl benzoate but avoidance was abolished in both genotypes following adaptation (), demonstrating the efficacy of the adaptation protocol. For cross-adaptation we tested separate groups of methyl benzoate adapted flies for pentyl acetate avoidance (). Wild-type flies adapted with methyl benzoate avoided pentyl acetate indicating that pentyl acetate activates additional OSNs in wild-type flies that do not respond to methyl benzoate. However, OR67a restored flies adapted with methyl benzoate also adapted their behavioral response to pentyl acetate, suggesting that pentyl acetate activates the same OSNs in these flies that respond to methyl benzoate. We also performed reciprocal cross-adaptation experiments where flies were adapted to pentyl acetate and tested for pentyl acetate or methyl benzoate avoidance behavior (). OR67a restored flies adapted to pentyl acetate also lost their response to methyl benzoate. In contrast, the same pentyl acetate pre-exposure partially altered the pentyl acetate and methyl benzoate response in wild-type flies. Therefore methyl benzoate and pentyl acetate activate overlapping OSNs in OR67a restored flies, again supporting the notion that odorants compete for a single relevant class of functional OSNs in OR67a restored flies.
OR67a restored flies cross-adapt to odorants that activate OR67a neurons
We also used cross-adaptation to test whether odorants that can be discriminated by OR67a restored flies, activate overlapping OSNs. Indeed, Or67a restored flies displayed reciprocal cross-adaptation to methyl benzoate and isoamyl acetate () and to pentyl acetate and 6-methyl-5-hepten-2-one (). In addition, we demonstrated that OR67a restored flies discriminate between methyl benzoate and pentyl acetate (Fig. S3
), two other odorants that they cross-adapt to. These data present further evidence that flies can discriminate between odorants using the same, and very likely a single class of, OSNs. Importantly, a purely spatial model for odorant encoding cannot account for discrimination between two odorants that activate the same class(es) of OSNs.
Flies with a single class of functional Or83b
-expressing OSNs could discriminate between odorants using odorant intensity (relative concentration) and/or identity (chemical structure) information. We therefore tested whether OR67a restored flies only coded odorant intensity by altering the concentration of one of the two odorants between training and testing discrimination. These manipulations simultaneously changed absolute concentration of one of the odorants and the relationship between odorants. We used pentyl acetate and 6-methyl-5-hepten-2-one because the odor-evoked firing rate of Or67a
-expressing OSNs to these odorants has been reported to vary between 10−2
). We first trained flies with either 10−2
dilutions of 6-methyl-5-hepten-2-one versus a constant 10−3
dilution of pentyl acetate and tested all groups for discrimination between 10−3
6-methyl-5-hepten-2-one versus 10−3
pentyl acetate (). Learned discrimination scores varied little for wild-type and OR67a rescued flies with changing 6-methyl-5-hepten-2-one concentration demonstrating that both wild-type and OR67a rescued flies identify 6-methyl-5-hepten-2-one, despite a change in absolute and relative odorant intensity. We similarly manipulated pentyl acetate concentration between training and testing. In this case, learned discrimination in wild-type and OR67a restored flies was robust when training concentration was lower, or the same, as that at test (). Both wild-type and OR67a restored flies performed most poorly when training concentration was higher than that at test. Since flies with restored OR67a neurons distinguish the appropriate odorant across changing concentration, they cannot be only coding absolute odorant intensity. Furthermore, since our experimental design also changed relative odorant intensity between training and testing, the flies do not utilize this parameter to discriminate odorants. Instead, these data suggest flies with restored OR67a neurons encode odorant identity.
Or83b2 flies with functional OR67a neurons discriminate odorants across changing concentration
In conclusion, multiple classes of OSNs are not required for flies to discriminate odorants. Although flies without functional Or83b-expressing neurons cannot learn to discriminate between a number of chemically distinct odorants, providing a single class of Or83b-expressing OSNs restores learned discrimination between two odorants that activate that particular OSN class. These flies cross-adapt to odorants that activate the restored OSNs demonstrating that the relevant OSNs are the same, thus challenging a requirement for discrete spatial codes for odorants in the antennal lobe. As expected, flies with one class of Or83b-expressing OSNs have limitations and can apparently only encode one odorant that activates the appropriate receptor at a time. These data suggest a benefit of having multiple classes of OSNs is the ability to identify certain odorants present within a more complex milieu. Importantly, Or83b2 mutant flies with one functional class of Or83b-expressing OSNs choose appropriately between two odorants even though the absolute and relative concentration is changed between training and testing, implying that they encode odorant identity, and do not only rely on encoding odorant intensity.
Finding that distinct combinatorial spatial patterns of OSN activation in the antennal lobe are not essential to represent odorant information implies an important role for odorant-evoked temporal dynamics. Previous studies in insects and vertebrates have documented considerable temporal complexity in odor-evoked activity at successive layers of the olfactory system (12
) but few have investigated the behavioral relevance (12
). Recent work has shown that excitatory and inhibitory lateral connectivity in the Drosophila
antennal lobe can shape projection neuron responses (21
), therefore we expect different temporal signals in the same OSNs to generate distinct temporal, and perhaps spatial, patterns of projection neuron activity. However, since flies with a single functional class of Or83b
-expressing OSNs lack the lateral input driven by additional classes of OSN, it will be important to determine how lateral connectivity within the antennal lobe contributes to odorant discrimination in Drosophila