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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
From:
J Pharmacol Exp Ther. Author manuscript; available in PMC 2009 September 1.
Published in final edited form as:
J Pharmacol Exp Ther. 2008 September; 326(3): 864–870.
Published online 2008 June 16. doi: 10.1124/jpet.107.135350

Figure 6

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BzATP augments inducible nitric oxide synthase (iNOS) protein expression induced by LPS in endothelium-intact vessels

The figure depicts a typical western blot image of iNOS protein expression (upper figure) and the statistical analysis of changes of iNOS protein expression in mouse endothelium-intact and -denuded aortic rings, which were incubated with DMEM [Control; E(+), n=3; E(-), n=3], LPS [100 μg/ml; E(+), n=3; E(-), n=3], BzATP [150 μM; E(+), n=3], LPS plus BzATP [LPS 100 μg/ml plus BzATP 150 μM; LPS+BzATP; E(+), n=3; E(-), n=3], oATP plus LPS plus BzATP [oATP 50 μM or 250 μM for 1 h before incubation with LPS plus BzATP; oATP (50 μM)+BzATP+LPS; E(+), n=3 or oATP (250 μM) +BzATP+LPS; E(+), n=3] and oATP [50 μM or 250 μM for 1 h before incubation with DMEM; oATP (50 μM); E(+), n=3 or oATP (250 μM); E(+), n=3]. Data are expressed as mean±SEM of n animals. *P<0.05 vs. LPS+BzATP in endothelium-intact vessels.

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