Search tips
Search criteria 


Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. 1991 October; 59(10): 3815–3818.
PMCID: PMC258956

Depletion of complement and effects on passive transfer of resistance to infection with Borrelia burgdorferi.


When irradiated hamsters are passively immunized with immune serum before challenge with Borrelia burgdorferi, they are completely protected from arthritis and infection. The complement dependency of this protection was addressed by treating hamsters with cobra venom factor. Depletion of complement abrogated the ability of immune serum obtained 1 and 10 weeks after infection to confer complete protection. By contrast, depletion of complement had no effect on the ability of 3-week immune serum to confer protection. These results suggest that complement-dependent, and possibly complement-independent, antibodies are important for preventing the induction of Lyme arthritis.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (777K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Banfi E, Cinco M, Perticarari S, Presani G. Rapid flow cytometric studies of Borrelia burgdorferi phagocytosis by human polymorphonuclear leukocytes. J Appl Bacteriol. 1989 Jul;67(1):37–45. [PubMed]
  • Benach JL, Fleit HB, Habicht GS, Coleman JL, Bosler EM, Lane BP. Interactions of phagocytes with the Lyme disease spirochete: role of the Fc receptor. J Infect Dis. 1984 Oct;150(4):497–507. [PubMed]
  • Benach JL, Habicht GS, Gocinski BL, Coleman JL. Phagocytic cell responses to in vivo and in vitro exposure to the Lyme disease spirochete. Yale J Biol Med. 1984 Jul-Aug;57(4):599–605. [PMC free article] [PubMed]
  • Callister SM, Case KL, Agger WA, Schell RF, Johnson RC, Ellingson JL. Effects of bovine serum albumin on the ability of Barbour-Stoenner-Kelly medium to detect Borrelia burgdorferi. J Clin Microbiol. 1990 Feb;28(2):363–365. [PMC free article] [PubMed]
  • Coe J, Peel L, Smith RF. The immune response in the hamster. V. Biologic activities of 7S-gamma-1 and 7S-gamma-2 globulins. J Immunol. 1971 Jul;107(1):76–82. [PubMed]
  • Duray PH. The surgical pathology of human Lyme disease. An enlarging picture. Am J Surg Pathol. 1987;11 (Suppl 1):47–60. [PubMed]
  • Fikrig E, Barthold SW, Kantor FS, Flavell RA. Protection of mice against the Lyme disease agent by immunizing with recombinant OspA. Science. 1990 Oct 26;250(4980):553–556. [PubMed]
  • Georgilis K, Steere AC, Klempner MS. Infectivity of Borrelia burgdorferi correlates with resistance to elimination by phagocytic cells. J Infect Dis. 1991 Jan;163(1):150–155. [PubMed]
  • Hejka A, Schmitz JL, England DM, Callister SM, Schell RF. Histopathology of Lyme arthritis in LSH hamsters. Am J Pathol. 1989 May;134(5):1113–1123. [PubMed]
  • Johnson RC, Kodner C, Russell M. Passive immunization of hamsters against experimental infection with the Lyme disease spirochete. Infect Immun. 1986 Sep;53(3):713–714. [PMC free article] [PubMed]
  • Johnson RC, Kodner C, Russell M. Active immunization of hamsters against experimental infection with Borrelia burgdorferi. Infect Immun. 1986 Dec;54(3):897–898. [PMC free article] [PubMed]
  • Johnson RC, Kodner C, Russell M, Duray PH. Experimental infection of the hamster with Borrelia burgdorferi. Ann N Y Acad Sci. 1988;539:258–263. [PubMed]
  • Johnson RC, Kodner CL, Russell ME. Vaccination of hamsters against experimental infection with Borrelia burgdorferi. Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):45–48. [PubMed]
  • Johnson RC, Marek N, Kodner C. Infection of Syrian hamsters with Lyme disease spirochetes. J Clin Microbiol. 1984 Dec;20(6):1099–1101. [PMC free article] [PubMed]
  • Kochi SK, Johnson RC. Role of immunoglobulin G in killing of Borrelia burgdorferi by the classical complement pathway. Infect Immun. 1988 Feb;56(2):314–321. [PMC free article] [PubMed]
  • Lovrich SD, Callister SM, Schmitz JL, Alder JD, Schell RF. Borreliacidal activity of sera from hamsters infected with the Lyme disease spirochete. Infect Immun. 1991 Aug;59(8):2522–2528. [PMC free article] [PubMed]
  • Newman K, Jr, Johnson RC. In vivo evidence that an intact lytic complement pathway is not essential for successful removal of circulating Borrelia turicatae from mouse blood. Infect Immun. 1981 Jan;31(1):465–469. [PMC free article] [PubMed]
  • Pavia CS, Kissel V, Bittker S, Cabello F, Levine S. Antiborrelial activity of serum from rats injected with the Lyme disease spirochete. J Infect Dis. 1991 Mar;163(3):656–659. [PubMed]
  • Peterson PK, Clawson CC, Lee DA, Garlich DJ, Quie PG, Johnson RC. Human phagocyte interactions with the Lyme disease spirochete. Infect Immun. 1984 Nov;46(2):608–611. [PMC free article] [PubMed]
  • Schaible UE, Kramer MD, Eichmann K, Modolell M, Museteanu C, Simon MM. Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Proc Natl Acad Sci U S A. 1990 May;87(10):3768–3772. [PubMed]
  • Schmitz JL, Schell RF, Hejka A, England DM, Konick L. Induction of lyme arthritis in LSH hamsters. Infect Immun. 1988 Sep;56(9):2336–2342. [PMC free article] [PubMed]
  • Schmitz JL, Schell RF, Hejka AG, England DM. Passive immunization prevents induction of Lyme arthritis in LSH hamsters. Infect Immun. 1990 Jan;58(1):144–148. [PMC free article] [PubMed]
  • Schmitz JL, Schell RF, Lovrich SD, Callister SM, Coe JE. Characterization of the protective antibody response to Borrelia burgdorferi in experimentally infected LSH hamsters. Infect Immun. 1991 Jun;59(6):1916–1921. [PMC free article] [PubMed]
  • Steere AC, Grodzicki RL, Kornblatt AN, Craft JE, Barbour AG, Burgdorfer W, Schmid GP, Johnson E, Malawista SE. The spirochetal etiology of Lyme disease. N Engl J Med. 1983 Mar 31;308(13):733–740. [PubMed]
  • Steiner BM, Schell RF, Harris ON. The effect of C3 depletion on resistance of hamsters to infection with the yaws spirochete. Sex Transm Dis. 1986 Oct-Dec;13(4):245–250. [PubMed]
  • Szczepanski A, Benach JL. Lyme borreliosis: host responses to Borrelia burgdorferi. Microbiol Rev. 1991 Mar;55(1):21–34. [PMC free article] [PubMed]
  • Winkelstein JA. Complement and the host's defense against the pneumococcus. Crit Rev Microbiol. 1984;11(3):187–208. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)