PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
 
Infect Immun. 1991 October; 59(10): 3523–3530.
PMCID: PMC258916

Sucrose-promoted accumulation of growing glucosyltransferase variants of Streptococcus gordonii on hydroxyapatite surfaces.

Abstract

Streptococcus gordonii exhibits a phase variation involving expression of high (Spp+) or low (Spp-) glucosyltransferase activity. The related bacterial accumulation on hydroxyapatite (HA) and saliva-coated HA surfaces was examined and found to be significant. Spp+ cells growing anaerobically in a defined medium utilize about 30% of the glucose available from sucrose to make insoluble glucans. These glucans formed cohesive masses on HA beads, which contained 80 to 90% of the total bacteria. The bacterial polymer mass had a volume of about 40 microns3 and contained more than 5 x 10(10) viable cells per cm3. In the absence of sucrose, the beads were saturated by 1 x 10(8) to 2 x 10(8) Spp+ cells. Spp- bacteria, which make 30-fold less glucan than do Spp+ bacteria, did not accumulate on surfaces in numbers significantly above the saturation level of 1 x 10(8) to 2 x 10(8) cells in the presence or absence of sucrose. Insoluble glucan synthesized by Spp+ cells from sucrose also enabled these bacteria to accumulate on saliva-coated HA seven times more effectively than the Spp- cells and 10 times more effectively than the Spp+ cells grown in medium without sucrose.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.7M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Appelbaum B, Golub E, Holt SC, Rosan B. In vitro studies of dental plaque formation: adsorption of oral streptococci to hydroxyaptite. Infect Immun. 1979 Aug;25(2):717–728. [PMC free article] [PubMed]
  • Bourgeau G, McBride BC. Dextran-mediated interbacterial aggregation between dextran-synthesizing streptococci and Actinomyces viscosus. Infect Immun. 1976 Apr;13(4):1228–1234. [PMC free article] [PubMed]
  • Buchan RA, Jenkinson HF. Glucosyltransferase production by Streptococcus sanguis Challis and comparison with other oral streptococci. Oral Microbiol Immunol. 1990 Apr;5(2):63–71. [PubMed]
  • Carlsson J, Grahnén H, Jonsson G, Wikner S. Establishment of Streptococcus sanguis in the mouths of infants. Arch Oral Biol. 1970 Dec;15(12):1143–1148. [PubMed]
  • Clark WB, Bammann LL, Gibbons RJ. Comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces. Infect Immun. 1978 Mar;19(3):846–853. [PMC free article] [PubMed]
  • Cowan MM, Parrish K, Kessler RE, Pyle C, Jr, Taylor KG, Ciardi JE, Doyle RJ. Glucan-binding factor in saliva. Infect Immun. 1988 Nov;56(11):2912–2917. [PMC free article] [PubMed]
  • Gibbons RJ. Bacterial adhesion to oral tissues: a model for infectious diseases. J Dent Res. 1989 May;68(5):750–760. [PubMed]
  • Gibbons RJ, Keyes PH. Inhibition of insoluble dextran synthesis, plaque formation and dental caries in hamsters by low molecular weight dextran. Arch Oral Biol. 1969 Jun;14(6):721–724. [PubMed]
  • Gibbons RJ, Houte JV. Bacterial adherence in oral microbial ecology. Annu Rev Microbiol. 1975;29:19–44. [PubMed]
  • Grahame DA, Mayer RM. The origin and composition of multiple forms of dextransucrase from Streptococcus sanguis. Biochim Biophys Acta. 1984 Apr 27;786(1-2):42–48. [PubMed]
  • Hamada S, Slade HD. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980 Jun;44(2):331–384. [PMC free article] [PubMed]
  • Hudson MC, Curtiss R., 3rd Regulation of expression of Streptococcus mutans genes important to virulence. Infect Immun. 1990 Feb;58(2):464–470. [PMC free article] [PubMed]
  • Kolenbrander PE, Andersen RN. Multigeneric aggregations among oral bacteria: a network of independent cell-to-cell interactions. J Bacteriol. 1986 Nov;168(2):851–859. [PMC free article] [PubMed]
  • LOE H, THEILADE E, JENSEN SB. EXPERIMENTAL GINGIVITIS IN MAN. J Periodontol. 1965 May-Jun;36:177–187. [PubMed]
  • Mayer RM. Dextransucrase: a glucosyltransferase from Streptococcus sanguis. Methods Enzymol. 1987;138:649–661. [PubMed]
  • McIntire FC, Vatter AE, Baros J, Arnold J. Mechanism of coaggregation between Actinomyces viscosus T14V and Streptococcus sanguis 34. Infect Immun. 1978 Sep;21(3):978–988. [PMC free article] [PubMed]
  • Niven CF, Kiziuta Z, White JC. Synthesis of a Polysaccharide from Sucrose by Streptococcus S.B.E. J Bacteriol. 1946 Jun;51(6):711–716. [PMC free article] [PubMed]
  • Nyvad B, Kilian M. Comparison of the initial streptococcal microflora on dental enamel in caries-active and in caries-inactive individuals. Caries Res. 1990;24(4):267–272. [PubMed]
  • Russell RR. Use of triton X-100 to overcome the inhibition of fructosyltransferase by SDS. Anal Biochem. 1979 Aug;97(1):173–175. [PubMed]
  • Schachtele CF, Harlander SK, Bracke JW, Ostrum LC, Maltais JA, Billings RJ. Streptococcus mutans dextransucrase: stimulation by phospholipids from human sera and oral fluids. Infect Immun. 1978 Dec;22(3):714–720. [PMC free article] [PubMed]
  • Tardif G, Sulavik MC, Jones GW, Clewell DB. Spontaneous switching of the sucrose-promoted colony phenotype in Streptococcus sanguis. Infect Immun. 1989 Dec;57(12):3945–3948. [PMC free article] [PubMed]
  • Terleckyj B, Willett NP, Shockman GD. Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infect Immun. 1975 Apr;11(4):649–655. [PMC free article] [PubMed]
  • van Loosdrecht MC, Lyklema J, Norde W, Zehnder AJ. Influence of interfaces on microbial activity. Microbiol Rev. 1990 Mar;54(1):75–87. [PMC free article] [PubMed]
  • YEMM EW, WILLIS AJ. The estimation of carbohydrates in plant extracts by anthrone. Biochem J. 1954 Jul;57(3):508–514. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)