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Glycogen synthase kinase-3 (GSK3), a serine/threonine kinase, is involved in diverse cellular processes ranging from nutrient and energy homeostasis to proliferation and apoptosis. Its role in glioblastoma multiforme (GBM) has yet to be elucidated.
We identified GSK3 as a regulator of GBM cell survival using microarray analysis, small molecule and genetic inhibitors of GSK3 activity. Various molecular and genetic approaches were then employed to dissect out the molecular mechanisms responsible for GSK3 inhibition-induced cytotoxicity.
We demonstrate that multiple small molecular inhibitors of GSK3 activity and genetic down-regulation of GSK3α/β significantly inhibit glioma cell survival and clonogenicity. The potency of the cytotoxic effects is directly correlated with decreased enzyme activity-activating phosphorylation of GSK3α/β Y276/Y216 and with increased enzyme activity-inhibitory phosphorylation of GSK3α S21. Inhibition of GSK3 activity results in c-MYC activation leading to the induction of Bax, Bim, DR4/DR5 and TRAIL expression and subsequent cytotoxicity. Additionally, down-regulation of GSK3 activity results in alteration of intracellular glucose metabolism resulting in dissociation of hexokinase-II (HKII) from the outer mitochondrial membrane with subsequent mitochondrial destabilization. Finally, inhibition of GSK3 activity causes a dramatic decrease in intracellular nuclear factor-kappa B (NF-κB) activity.
Inhibition of GSK3 activity results in c-MYC dependent glioma cell death through multiple mechanisms, all of which converge on the apoptotic pathways. GSK3 may therefore be an important therapeutic target for gliomas. Future studies will further define the optimal combinations of GSK3 inhibitors and cytotoxic agents for use in gliomas and other cancers.
Glioblastomas (GBM) are among the most lethal tumors. New therapeutic approaches utilizing novel molecular targets are clearly needed. In the course of conducting a clinical trial of enzastaurin (LY317615.HCl), a new small molecule inhibitor of protein kinase C-beta (PKCβ)(1), we noted a number of impressive radiographic responses in patients with recurrent glioblastomas, consistent with a primary cytotoxic mechanism of action rather than, or in addition to, a pure anti-angiogenic mechanism of the drug (2). While attempting to elucidate the apparent cytotoxic activity of enzastaurin against gliomas, we discovered that the alpha and beta forms of GSK3 were targets of the drug in addition to its primary target, PKCβ, (Figure S1). In this study we therefore evaluated whether GSK3 may be a potentially new therapeutic target in gliomas.
GSK3 was initially identified over 25 years ago as a protein kinase that phosphorylated and inactivated glycogen synthase (GYS) (3), the final enzyme in glycogen biosynthesis. Recently it has been recognized as a key component of a diverse range of cellular functions essential for survival (for reviews see (4, 5). The role of GSK3 in the regulation of apoptosis is controversial. GSK3β knockout mice are embryonically lethal, and mouse embryonic fibroblasts (MEFs) derived from these embryos are sensitized to apoptosis (6). However, this observation appears to contradict the finding that overexpression of GSK3 is sufficient to induce apoptosis (7). A recent report has demonstrated that GSK3 is a pro-survival factor in pancreatic tumor cells, partly through its ability to regulate the NF-κB pathway (8). These data are consistent with the recent demonstration that GSK3β can regulate NF-κB stability and activity (6, 9). Finally, new experimental evidence suggests that GSKβ inhibition can promote Bax-mediated apoptosis in colorectal cancer cells (10). These data confirm the complex role of GSK3 in apoptosis and demonstrate that the biological outcome of GSK3 signaling is cell type and tissue context-dependent.
Little is currently known regarding the significance of GSK3 to glioma cell survival. We therefore sought to evaluate the role of GSK3 in glioma cell survival, proliferation and tumorigenicity both in vitro and in vivo and on the mechanistic consequences of inhibiting GSK3 activity. Our data point to GSK3 as a potentially attractive new target for human glioma therapy.
The glioma cell lines, U251, T98, U87, U373, U118 and A172, were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and cultured according to ATCC protocols.
Enzastaurin was kindly provided by Eli-Lilly. Enzastaurin, Lithium chloride and Kenpaullone (Sigma, St. Louis, MO) were dissolved in 100% DMSO and utilized at the concentrations specified in the manuscript. The GSK3 inhibitor, LY2064827, was kindly provided by Eli-Lilly whereas the GSK3 inhibitors 705701, 708244 and 709125 were kindly provided by The Developmental Therapeutics Program, The National Cancer Institute (Bethesda, MD). Recombinant human TRAIL was from R&D Systems (McKinley Place, MN).
Cell proliferation assays were performed by cell counting using Trypan Blue staining for dead cell visualization. Alternatively, a fluorescent assay based on Alamar Blue (BioSource, Camarillo, CA) reduction by viable cells was performed according to the manufacturer's manual.
siRNA duplexes were synthesized by Dharmacon (Chicago, IL): GSK3α (M-003009−01); GSK3β (M-003010−03); NF-κB p65 (cat# MQ-003533−01); c-MYC (cat# L-003282−00). Oligofectamine (Invitrogen, Carlsbad, CA) was utilized for transfection of siRNA into cells per the manufacturer's instructions. shRNA clone for c-MYC V2HS_152050 was from Open Biosytems (Huntsville, AL). Cells stably overexpressing pcDNA6 (Invitrogen) and TMP-MYC-shRNA were selected with 5 μg/ml blasticidin, 1 μg/ml puromycin.
U251 cells were plated at a density of 750,000 in 15 cm tissue culture dishes 24 hours before treatment with enzastuarin started. After 2 hour pulse with 10 mM BrdU (BD Biosciences Pharmingen, San Diego, CA) BrdU-labeled cells were detected as indicated in the user's manual. FACS analysis was performed using FACS-Vantage SE flow cytometer and Cell Quest Acquisition and Analysis software (BD Biosciences).
U251 cells were treated in triplicates with 10μM enzastaurin for 4, 10 and 18 hours. Total RNA was isolated using TRIZOL (Invitrogen) and further purified using RNeasy Mini Kit (Qiagen, Valencia, CA). Samples were hybridized to Affymetrix Human Genome U133 Plus 2 GeneChip Arrays (Affymetrix, Santa Clara, CA) according to the Affymetrix GeneChip Expression Analysis Technical Manual. The initial gene expression analysis data files (CEL files) were generated using Affymetrix GeneChip Operating Software (GCOS) version 1.2. CEL files were processed as described earlier (11). The false discovery rate of 0.05 and 1.5-fold change were applied as thresholds to select up- or down- regulated probes. Data were analyzed by Ingenuity pathway software analysis. Accession number of the files submitted to NCBI GEO is GSE11285. The sample sets used in figure 8 were previously published in (11, 12).
mRNA expression levels were quantified in triplicates by real-time RT-PCR on an ABI Prism 7900 sequence detection system (Applied Biosystems, Foster City, CA). The relative amount of target transcripts quantified by standard curve method was normalized to the amount of human GAPDH or actin transcripts found in the same sample. Primers and probes were purchased from Applied Biosystems.
Whole cell lysates were prepared in Cell Lysis Buffer (Cell Signaling Technology Inc., Bevely, MA). Cell lysate samples were run on a pre-cast 4−12% Bis-Tris gel (Invitrogen), transferred to a PVDF membrane (Invitrogen) and probed with the following antibodies: anti-phospho S21/S9 GSK3α/β, (1:2000; Cell Signaling), anti-phGKS3βSer9 and anti-phGSK3βY216 (1/1000; BD Biosciences) anti-β-catenin and anti-phospho-β-cateninSer33,Ser37,Thr41, anti-phospho c-MYC, T58 (1:1000; Cell Signaling), anti-c-IAP1, anti-c-IAP2 and anti-TRAIL (1/1000; BD Biosciences), anti-GAPDH, anti-c-MYC, N262, NF-κB (1/500) (Santa Cruz Biotechnology, Palo Alto, CA), anti-NF-κBp65 (1/1000; Cell Signaling)β-actin antibody (1:200, Santa Cruz Biotechnology, Santa Cruz, CA). Anti-DR4 and DR5 antibodies were from Chemicon (Temecula, CA). Anti-glycogen antibody and anti-phosphoS62 c-MYC were generous gift from Dr. O. Baba (13, 14) and Dr. R. Sears respectively. HRP-conjugated secondary antibodies to rabbit IgG, mouse IgG (1:5000; Cell Signaling), or goat-IgG were used (1:10000; Jackson Immunology Labs, Bar Harbor, ME). All antibodies were diluted in blocking buffer (5% w/v BSA (Sigma), 10mM Tris-HCl, 100 mM NaCl, 0.1% v/v Tween-20). All other buffers used during Western blotting were made following Invitrogen's NuPage protocol.
To measure NF-κB and TCF activity in glioma cell lines we utilized a NF-κB/luciferase reporter plasmid (Stratagene, La Jolla, CA) and pTOPflash reporter plasmid (Upstate, Luzern, Switzerland), respectively. To normalize transfection efficiency, a CMV-βgal plasmid was co-transfected. Reporter activity was detected 24 hours after transfection with the luciferase assay system (Promega Corp., Madison, WI) or β-galactosidase assay system (Promega) on a 20/20n Luminometer (Turner Biosystems, Sunnyvale, CA). c-MYC activity assays were performed with ELISA-based c-MYC activity assay kit (Active Motif, Carlsbad CA) according to the manufacturer's instructions.
GSK3α and GSK3β kinase activities were measured with the Kinase Profiler Assay Protocol by Upstate (Temecula, CA). Hexokinase activity assays were performed as described elsewhere (15).
Colony formation assays were performed using the In vitro tumorigenicity Assay kit from Clontech (Mountain View, CA) according to the manufacturer's instructions. One thousand cells were plated in the upper agar layer per well of 12-well plates. Three or four weeks later colonies were stained and counted.
U87 cells were injected intracranially in neonatal SCID mice or 6−8 week old athymic nude mice (NCI-Frederick Animal Production Area -APA- MD). Stereotaxic coordinates, for injection in adult animals, were 2.8 mm distal to the midline, 1 mm anterior to the coronal suture and 2.5 mm deep from the dura. All procedures were in accordance with NIH Animal Care and Use Committee protocols. Fifteen days after tumor cells injection, enzastaurin (75 mg/kg) and/or carboplatin (40 mg/kg) were administered. Control animals were treated with vehicle alone. For evaluation of tumorigenicity animals were observed daily for death or signs of distress. Kaplan-Meier analysis was performed using Prizm 4 (GraphPad Software Inc., San Diego, CA) or JMP 5.1 (SAS Institute, Cary, NC). Subcutaneous tumor models were generated by injection of 500,000 siRNA-treated U251 cells subcutaneously on the right thigh of 6−8 week old athymic male nude mice. Tumors were measured twice a week.
After we found that enzastaurin exerts direct anti-proliferative and apoptotic effects in vitro on multiple glioma cell lines (U87, T98, U251, 373) as well as on primary glioma tumor stem cell (TSC)-like lines (TSC0308 and TSC1228) at therapeutically achievable concentrations (Fig. 1A, ,2D,2D, S11 and data not shown), we addressed the mechanism of enzastaurin-induced cytotoxicity through analysis of global gene expression changes over time. Unexpectedly, we found that enzastaurin abnormally regulated expression of a number of WNT pathway genes; Axin 2 showing the most significant change with about 100-fold induction (Table S1, Fig.S1E Other affected genes and pathways, as detected by Anova and Ingenuity Pathway Analysis software, are shown in Tables S2 and S3 respectively). We confirmed activation of the WNT pathway through the demonstration of enzastaurin-mediated loss of β-catenin S37/41 phosphorylation with resultant β-catenin accumulation in the cytoplasm and subsequent translocation to the nucleus (Fig. S1 A-C). This resulted in a dose-dependent increase in the transcriptional activity of β-catenin (Fig. S1, D and E).
Since GSK3 is known to be a major regulator of β-catenin S37/41 phosphorylation, we asked whether enzastaurin modulates GSK3 activity. We found that the activation-related phosphorylation of Y216/Y276 in GSK3α/β was dramatically decreased following exposure to enzastaurin (Fig. 1B), while inhibitory phosphorylation of GSK3α S21 was significantly up-regulated. Furthermore, the enzymatic activity of both forms of GSK3 was directly down-regulated by 0.5 μM enzastaurin (lowest concentration tested) to less than 3% of the activity of the wild type control enzyme as demonstrated by direct in vitro activity assays (Fig. S2).
We next evaluated whether inhibition of GSK3 by other GSK3 inhibitors resulted in glioma cell cytotoxicity. Along with the relatively non-selective GSK3 inhibitors, lithium chloride (LiCl) (16, 17) and kenpaullone (18) (Fig. S3A-B), we also evaluated several highly selective small molecule GSK3 inhibitors (705701, 708244, 709125 and LY2064827). Serum-cultured U251, U87 and T98 cell lines and two glioma tumor stem cell (TSC)-like lines (11) were analyzed following treatment with each GSK3 inhibitor (Fig S3C). Each drug exerted cytotoxic effects on glioma cell lines in a dose- and time-dependent manner; the most potent of which, LY2064827, demonstrated significant cytotoxicity at concentrations as low as 0.1 μM (Fig S3C). Moreover, the potency of each drug's cytotoxic effects correlated directly with level of decreased phosphorylation of GSK3α/β Y276/Y216 and with increased phosphorylation of GSK3α S21 (Fig. 1B). These results suggest that the cytotoxic effects of these drugs are directly related to their ability to inhibit GSK3 activity.
To further address the “on-target” specificity of the cytotoxic effects of the GSK3 inhibitors, we examined whether direct down-regulation of GSK3 protein expression inhibits glioma cell growth. GSK3 siRNA-mediated down-regulation of GSK3 resulted in decreased U251 and T98 glioma cell growth (Fig. 1C and data not shown). Furthermore, we observed a 70% reduction in U251 glioma cell clonogenicity as revealed by colony formation assay in soft agar (Fig. 1C). Moreover, there was significant growth delay from GSK3 siRNA treated gliomas in vivo compared to siRNA-control treated cells (Fig. 1D). Down-regulation of GSK3 activity by shRNA also resulted in decreased tumor growth in another mouse xenograft model (Fig. S12).
Since GSK3 is involved in cellular energy metabolism, we investigated the effects of GSK3 inhibition on several parameters of glioma cell glucose metabolism. GSK3 siRNA and enzastaurin significantly inhibited the phosphorylation of the S640 residue of glycogen synthase (GYS), thereby releasing the normal inhibitory effects of GSK3 on GYS (Fig. 2A and S4A) and resulting in increased intracytoplasmic glycogen storage and decreased cytoplasmic glucose concentrations (Fig. 2B).
Majewski et al. (15) have demonstrated that a drop in intracellular glucose levels negatively affects the association of Hexokinase (HK) activity with mitochondria, resulting in cellular apoptosis. We, therefore, evaluated the effects of GSK3 inhibition on mitochondrial HK activity. Mitochondrial HK activity decreased by two-fold following GSK3 siRNA or a 4 hour exposure to 5 μM enzastaurin (Fig. S4B-C). By contrast, total and mitochondrial-associated Bax expression significantly increased in the cells treated with GSK3 siRNA or enzastaurin (Fig.2C and S4D), resulting in an increase in the mitochondrial-associated Bax/HKII ratio. (Fig. 2D). Displacement of HKll activity from mitochondria along with increased mitochondrial-associated Bax protein has been shown to lead to a loss of mitochondrial membrane integrity with resultant apoptosis (19, 20). Indeed, mitochondrial Cytochrome C release was detected and MitoTracker® staining revealed a significant loss of functional mitochondria in enzastaurin, 705701, LY2064827 and GSK3-siRNA treated glioma cells consistent with mitochondrial-mediated cell death (Fig. 2D, and data not shown). The importance of mitochondrial HKll activity in glioma cell survival is supported by the observation that HKll-siRNA mediated down-regulation of HKll induced glioma cell death (Fig. S4E).
We and others have previously demonstrated the importance of pro-survival activities of NF-κB in glioma (21-25). Recent studies have linked inhibition of GSK3β to negative regulation of NF-κB activity (6). We found that NF-κB was inhibited by LiCl, kenpaullone, enzastaurin as well as by GSK3 si RNA treatments in U251T98 and U87 (Fig. 3A-B and data not shown). GSK3 inhibition was accompanied by down-regulation of a number of NF-κB regulated pro-survival genes including IL8, IER3, and BIRC2 as assessed by microarray gene expression analysis and TaqMan RT-PCR (Fig. S5A-C). Moreover, NF-κB inhibition resulted in decreased glioma cell survival in vitro and in inhibition of tumor growth in vivo (Fig. 3C and Fig. 1C). Finally, we also show that up-regulation of NF-κB activity by stable overexpression of NF-κB (p65) results in a partial protection of U251 against GSK3 inhibition-induced cell death (Fig. S9). Thus, GSK3 inhibition contributes to glioma cell death through negative regulation of a number of NF-κB associated pro-survival genes.
Western blot analysis demonstrated that DR4 (TNFRSF10A), DR5 (TNFRSF10B) proteins and TRAIL were significantly induced by GSK3 inhibition in a time- and a dose-dependent manner (Fig. 4A-C and data not shown). Since c-MYC activity is partially regulated by GSK3, and since c-MYC is known to regulate the transcription of DR5 (26, 27), we evaluated c-MYC activity following GSK3 inhibition. An ELISA-based DNA-binding assay demonstrated that c-MYC activity was up-regulated after 4 hours of enzastaurin treatment and by GSK3 siRNA (Fig. 5A).
The stability and thus activity of c-MYC is specifically regulated by differential phosphorylation of its N-terminus through GSK3-mediated inhibitory phosphorylation of c-MYC T58 and through ERK1/2 activating phosphorylation of c-MYC S62 (28-30). We found that both enzastaurin and GSK3 siRNA induced loss of the inhibitory phosphorylation of c-MYC at T58 and increased phosphorylation of the activating phosphorylation site S62 (Fig. 5B, S10 and data not shown). Consistent with increased c-MYC S62 phosphorylation, treatment with different GSK3 inhibitors induced ERK1/2 phopshorylation (Fig. S6). ERK1/2 activation was sustained (Fig. S6A) and dose-dependent (Fig. S6B) suggesting that ERK1/2 might be responsible for the phosphorylation of c-MYC S62 in glioma cells as has been reported previously for other cell types (28).
We next examined whether c-MYC regulates transcription of Bax and TRAIL-associated death receptors in glioma cell lines, as has been demonstrated in other cells (for a review see (31)). Treatment of glioma cells with c-MYC siRNA resulted in significant down-regulation of Bax, DR4 and DR5 both at the RNA (Fig. S7A-C) and protein levels (Fig. 5C). Moreover, down-regulation of c-MYC expression, by stable transfection of a c-MYC shRNA in U251, resulted in almost complete protection against GSK3 inhibitors cytotoxicity (Fig. 5D). Consistent with the cytotoxicity assays, 40% of parental U251 cell mitochondria completely lost their transmembrane potential 24 hours after exposure to 705701, whereas an insignificant percentage of cells lost their mitochondria transmembrane potential in the c-MYC shRNA-stably transfected glioma cells exposed to 705701 (Fig. S7H). By contrast, c-MYC overexpression was cytotoxic to glioma cells (Fig. 6B) and positively regulated transcription of Bax, DR4 and DR5 (Fig. S7D-F).
Finally, we demonstrate that GSK3 inhibition resulted in significantly increased levels of both Bim RNA and protein, a known Bcl-2 antagonist and c-MYC target gene (Fig. S7I-J and data not shown) (32). These observations are consistent with the hypothesis that c-MYC plays a significant, if not predominant, role as a mediator of GSK3 inhibition-induced glioma cell apoptosis.
We found that in addition to DR4/5, TRAIL was up-regulated by enzastaurin treatment and GSK3 siRNA (Fig. 4C) in several glioma cell lines. Given that glioma cells are relatively resistant to TRAIL-induced apoptosis and that GSK3 inhibition up-regulates the death receptors, Bax and TRAIL, and inhibits the anti-apoptotic HKll/mitochondrial and NF-κB mechanisms, we examined whether GSK3 inhibition would result in increased sensitivity to apoptotic stimuli such as TRAIL itself. We found that TRAIL-mediated cell death was dramatically enhanced when otherwise sub-cytotoxic doses of TRAIL (25−50 nM) and enzastaurin (1 − 2.5 μM) were combined in U251 (Fig. 6A) and U87 (data not shown) cell lines. Similar data were obtained with other GSK3 inhibitors 705701 (0.05−0.1μM) and LY2064827(0.05 μM) as well as with GSK3 siRNA (data not shown). Moreover, overexpression of c-MYC rendered cells more sensitive to TRAIL-mediated cytotoxicity (Fig. 6B), again supporting the hypothesis of the important role of c-MYC in GSK3-induced cytotoxicity. Finally, enzastaurin combined with carboplatin, a standard DNA damaging chemotherapeutic agent often used in patients with malignant gliomas, causes synergistic tumor killing both in vitro and in vivo (Fig. 6C and D) confirming our hypothesis that GSK3 inhibition would have additive/synergistic cytotoxic activity in combination with other, unrelated DNA damaging agents.
Although initially viewed as a specific regulator of glycogen metabolism, GSK3 has recently been shown to be a crucial enzymatic regulator of a diverse number of cellular functions including cell structure, metabolism, and survival (reviewed in (4, 5)). Our work demonstrates a role for GSK3 inhibition in mediating glioma cell proliferation arrest, decreased clonogenicity and induction of apoptotic cell death through both the extrinsic and intrinsic apoptotic pathways both in vitro and in vivo, much of which is mediated through c-MYC.
c-MYC is known to play important roles in the regulation of cell proliferation, differentiation and apoptosis and is deregulated in many human tumors (33, 34), including glioma (35-37) (Fig. S8). c-MYC can be activated by phosphorylation at S62 by MAP kinase ERK1/2 (extracellular signal-related protein kinase2) whereas it is inhibited by GSK3-mediated phosphorylation at T58 (28). We show that the increase in c-MYC activity in glioma cells after GSK3 inhibition correlates with a loss of T58 phosphorylation and with an increase in S62 phosphorylation. The decrease in c-MYC phosphorylation at T58 observed following enzastaurin or GSK3 siRNA treatment correlates with the inhibition of Tyrosine 216/276 phosphorylation of GSK3α/GSK3β and is therefore likely to be a direct result of decreased GSK3 activity. By contrast the increase in c-MYC S62 phosphorylation coincides with an increase in activating ERK1/2 phosphorylation consistent with the recent demonstration that GSK3 negatively regulates ERK1/2 activity (38). Thus, inhibition of GSK3 activity results in increased phosphorylation and activity of ERK1/2, as we demonstrated in figure S6. In addition to a likely direct effect of ERK1/2 on activation of c-MYC, sustained phosphorylation of ERK1/2 and its target p90rSK have been shown to induce cell cycle arrest (39), as we observed in our enzastaurin and GSK3 siRNA treated cells (Fig. S6 and data not shown). Seth and co-workers have suggested that the elevated Cdk1/CyclinB levels seen in G2/M may mediate the increased S62 phosphorylation of c-MYC observed in G2/M (40). This is consistent with our data demonstrating up-regulation of Cyclin B following enzastaurin and GSK3 siRNA induced G2/M arrest prior to induction of apoptosis (data not shown). Whether GSK3-inhibition-induced phosphorylation of c-MYC S62 is mediated directly by ERK1/2 and/or G2/M arrested cellular up-regulation of Cdk1/cyclin B remains a subject of future studies.
GSK3 inhibition-mediated up-regulation of c-MYC activity results in increased expression of apoptosis-related molecules DR5, Bim, Bax and TRAIL and down-regulation of anti-apoptotic protein, FLIP (data not shown), all known targets of c-MYC (27, 31). DR4 may also be a target of c-MYC transcriptional activity in glioma cells since siRNA-mediated down-regulation of c-MYC resulted in decreased mRNA and protein levels of DR4 whereas overexpression of c-MYC up-regulates DR4 (Fig. S7). Our demonstration that reduction of c-MYC activity by shRNA protects glioma cells from GSK3-mediated cytotoxicity, together with data reported by Rottman and co-workers, suggests that c-MYC plays a major role as a mediator of GSK3 inhibition-related induction of TRAIL-mediated apoptosis (41).
In addition to induction of apoptosis through a TRAIL-mediated mechanism, our data also demonstrates that GSK3 inhibition may increase the propensity for glioma cell death through direct destabilization of the mitochondrial membrane. HK can inhibit apoptosis-related mitochondrial Cytochrome C release by interfering with the ability of Bax to bind to the outer mitochondrial membrane (19). We show that treatment of glioma cells with GSK3 inhibitors results in increased mitochondria - associated Bax protein, accompanied by a decrease in mitochondria - associated HKII activity (Fig. 2 and S4). Increases in the Bax/HKll ratio on the outer mitochondrial membrane have been previously demonstrated to result in loss of mitochondrial membrane potential with subsequent mitochondrial Cytochrome C release and apoptosis, as we observed following GSK3 inhibition in glioma cells.
Although the exact mechanism for HK dissociation from mitochondria following GSK3 inhibition remains the subject of future experiments, it has been previously demonstrated that reduction in intracellular glucose content induces dissociation of HK from mitochondria (15). Thus, it is likely that the nearly two-fold reduction in intracellular glucose concentration we observed in glioma cells following GSK3 inhibition is at least partially responsible for the HK dissociation from mitochondria. The reduction in intracellular glucose content following GSK3 inhibition is likely a result of decreased GSK3-dependent inhibitory GYS S640 phosphorylation resulting in over-stimulation of GYS activity and leading to the increased intracellular glycogen content we observed.
Finally, we demonstrated that down-regulation of GSK3 activity led to a decrease in NF-κB activity within glioma cells; an important finding since down-regulation of NF-κB by siRNA or by pharmacological means (salicylates, velcade) results in increased propensity for glioma cell death ((23) and unpublished data). These observations are in agreement with those from other groups who have demonstrated regulation of MEF and pancreatic cancer cell survival by GSK3β through an NF-κB-dependent pathway (6). Whether the effect of GSK3 inhibition on NF-κB activity in glioma cells is mediated by direct phosphorylation of NF-κB components as previously described (9, 42), or through the upstream regulators, IKK and IKB (43), and/or through inhibitory binding by increased nuclear β-catenin (44, 45) remains a matter of further study.
It is interesting to note that a significant number of GBMs (as well as many other cancer types) have mutations/deletions of the PTEN gene locus leading to constitutive AKT activation. This likely results in constitutive down-regulation of GSK3 activity through S9 phosphorylation by AKT (46). This constitutive inhibition of GSK3 activity may in part be responsible for the up-regulation c-MYC activity in gliomas (Figure S8) and a number of other tumors that do not have amplification of the c-MYC gene (35-37). The higher basal level of c-MYC activity found in glioma and tumor cells may subsequently make them significantly more sensitive to c-MYC-induced apoptosis following application of GSK3 inhibitors compared to normal cells that start out with low levels of c-MYC activity. Thus, it is plausible that deregulation of the PI3K pathway and activation of AKT may be a synthetic lethal event for further GSK3 inhibition. If so, patients suffering from tumors with constitutive active AKT may ultimately prove to be preferentially sensitive to therapeutic GSK3 inhibition. Additional in vitro and in vivo experimental data will clearly be needed to test this hypothesis.
In conclusion, we have demonstrated that GSK3 inhibition in glioma cells leads to; i, induction of pro-apoptotic effects through priming of the TRAIL death receptor pathway (GSK3/c-MYC/DR4/DR5/TRAIL/Bim); ii, inhibition of pro-survival signals through inhibiting NF-κB activity; iii, induction of mitochondrial permeability (Bax/HKll ratio) through alteration intracellular glucose regulation (GSK3/GYS). These data suggest that GSK3 may be an important molecular target for human glioma therapy. Future studies will need to better define the optimal combinations of GSK3 inhibitors and cytotoxic agents in additional models of glioma and other cancers in order to better understand and optimize the most promising therapeutic strategies to evaluate in the clinic.
We acknowledge The Developmental Therapeutics Program, The National Cancer Institute (Bethesda, MD) and Dr. Zaharevitz for kindly provided GSK3 inhibitors 705701, 708244 and 709125. We are thankful to Dr. Sears for providing us with an aliquot of anti-phospho S62 c-MYC antibody, to Dr. Baba for a generous gift of the anti-glycogen-specific antibody, and to Dr. Nogueira for sharing her detailed protocol with us on Hexokinase activity assays (NF-κB plasmid was kindly provided by Drs. Bren and Paya from Mayo Clinic College of Medicine). We also thank Mr. James W. Nagle and Ms. Deborah Kauffman from NINDS sequencing facility and Dr. Carolyn L. Smith from NINDS light image facility for their valuable contributions to this work. This research was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.
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Figure S1. Wnt pathway is induced by enzastaurin treatment. (A) Western blot demonstrating that enzastaurin down-regulates GSK3 dependent phosphorylation of β-catenin at S33/S37/T41 in U251 in a time-dependent manner. (B) Western blot showing accumulation of β-catenin in U251 cells at 24 hours after 5 μM enzastaurin treatment. (C) Immunohistochemistrydemonstrates time-dependent redistribution of β-catenin (green) inside T98 glioma cells from inter-cellular junctions (a, b) into cytoplasm and nucleus (c,d) after 10 μM enzastaurin treatment. (D) TCF reporter luciferase assay in U251 reveals dose-dependent reporter activation, reaching up to 200-fold induction of luciferase activity at 10 μM enzastaurin. (E) Real-time PCR confirms dose-dependent over-expression of AXIN2 mRNA induced by enzastaurin in U251.
Figure S2. Enzastaurin inhibits GSK3 activity. Enzastaurin down-regulates GSK3 activity for both GSK3 forms as detected by in vitro assay. The kinase activity of the drug at the indicated concentrations was measured by in vitro radioactive assay using a GSK3 recombinant enzymes and the phospho-GS2 peptide as substrate. Decrease in activity from control (0 μM) for all concentrations is statistically significant with p<0.001 as revealed by t-test.
Figure S3. Cytotoxic effect of GSK3 inhibitors. (A) LiCl stimulates cell death in U251 cells in a dose-dependent manner as evaluated by the AlamarBlue Viability Assay. Cells were incubated at indicated concentrations of LiCl for 48 hours (B) U251 cells were incubated with kenpaullone at the indicated concentrations for 48 hours Proliferation of the cells was evaluated by cell counting. (C) Three glioma cell lines – U251, T98 and U87 (plated at a cell density 30,000 per a well of a 6-well plate) – and two primary glioma TSC-like lines, 0308 and 1228, were tested with 4 GSK3 inhibitors at concentrations ranging from 0.1 μM to 150 μM for 3−7 days as indicated.
Figure S4. GSK3 inhibition is mediated by changes in glycogen metabolism and anti-apoptotic mechanisms in mitochondria. (A) Western blot of enzastaurin-treated U251 cells shows that GYS phosphorylation at S640 is decreased in a dose-dependent manner (24 hours of treatment). This correlates with the decrease of GSK3's Y216/276 phosphorylation. The only exception is that LiCl does not cause inhibition of pY276/216, as do all of the other GSK3 inhibitors we tested. We believe the reasons for this is that LiCl is a less specific drug relative to its off-target effects on the cell and has a different mechanism of GSK3 inhibition than the other drugs we looked out. Specifically, LiCl appears to inhibit GSK3 acting as a competitive inhibitor of Mg and through PP1 inhibition and/or AKT activation whereas all of the other compounds we evaluated are direct ATP-competitive inhibitors. Consistent with our data, others have shown that LiCl does not result in a change in Y216/279 phosphorylation by LiCl. Hexokinase assays of mitochondrial fractions of U251 cells treated with enzastaurin, 5μM for 4 hours (B) or with GSK3 siRNAs (C). Western blot analysis of mitochondrial and cytosolic fractions of U251 and T98 cell lines treated with enzastaurin, 5μM (D). (E) Cytotoxicitiy of HKII siRNA treatment in U251, NS, control non silencing siRNA (48 hours after transfection).
Figure S5. GSK3 inhibition leads to decrease in NF-κB transcriptional activity in glioma cells and to down-regulation of NF-κB target genes. Results of real-time PCR show down-regulation of levels of IER3, BIRC2 and IL8 mRNAs in U251 after 1 hour (IER3) and 4 hours (BIRC2 and IL6) of treatment with 5μM enzastaurin.
Figure S6. ERK1/2 phosphorylation is up-regulated with enzastaurin treatment in glioma cells. (A) Western blot analysis of total cell lysates revealed time- (A) and dose-dependent (B) phosphorylation of ERK1/2 with enzastaurin. This increase correlated directly with level of decreased activity-related phosphorylation of GSK3α/β Y276/Y216. Treatment time in B is 24 hours. These results suggest that the up-regulation of ERK1/2 phosphorylation is directly related to the inhibition of GSK3 activity. As a confirmation of this hypothesis, other GSK3 inhibitors kenpaullone (Kp, 5μM), LY2064827 (0.5μM) and enzastaurin (5μM) (C) also increase ERK1/2 phosphorylation in U251, as measured at 24 hours of treatment. As shown in A, phosphorylation of p90rsk, the target of ERK is also up-regulated. It was previously described that prolonged sustained phosphorylation of ERK1/2 and its target p90rSK induced cell cycle arrest (39), as we observed in our enzastaurin and GSK3 siRNA treated cells.
Figure S7. c-MYC regulates mRNA levels of Bax, DR4, DR5 and Bim and mitochondria anti-apoptotic mechanism. Results of real-time PCR show that the levels of DR4 (A, D), DR5 (B, E) and Bax (C, F) mRNAs are down-regulated by c-MYC siRNA in U251 and up-regulated by c-MYC overexpression, respectively. (G) The graph shows quality of silencing of c-MYC at mRNA level, as detected by Real-Time TaqMan RT-PCR. (H) c-MYC shRNA protects U251 glioma cells from 705701 by inhibiting mitochondria-mediated apoptosis. GSK3 inhibitors LY2064827 and 7050701 upregulate Bim protein (I) and RNA (J) levels. All data are shown for 48 hours of treatment.
Figure S8. Constitutive high c-MYC activity in high-grade gliomas. Combination dot/box-and-whisker plot of c-MYC mRNA expression (Affymetrix Human Genome U133 Plus 2 arrays, probe set 202431_s_at) in 180 fresh frozen glioma samples The sample set contains 76 glioblastomas, 29 astrocytomas, 42 oligodendrogliomas, 12 mixed oligoastrocytomas and 21 non-tumor samples (N) (temporal lobe resection of epileptic patients).
Figure S9. Overexpression of p65 partially protects glioma cells from enzastaurin toxicity. U251 cells were stably transfected with p65 expressing plasmid. NF-κB activity (A) was measured using a luciferase reporter assay in both control and p65 cells 24 hours after the cells were treated with 2.5 μM enzastaurin. (See Materials and Methods for details on reporter assays). p65 overexpression rendered U251 cells less sensitive to enzastaurin as shown by cell counts (B). Levels of NF-κB, p65 are shown in C.
Figure S10. c-MYC is up-regulated by enzastaurin.
Figure S11. Caspase 3 cleavage in U251 after 24 hours treatment with enzastaurin.
Figure S12. Subcutaneous tumor xenograft nude mouse model. Five million U251 control cells or U251-GSK3β-shRNA cells were injected subcultaneously. Tumors were measured twice a week. Tumors derived from U251-GSK3β-shRNA grow slower than those derived from control U251.
Table S1. WNT pathway – responsive genes are deregulated by enzastaurin in U251