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Conceived and designed the experiments: SLE MT JW PC AWB. Performed the experiments: SLE SR SL NSH NL MT JG NK JW DJS. Analyzed the data: SLE SR SL PC AWB. Wrote the paper: SLE. Performed critical editing of the paper: AWB.
Dishevelled (Dvl) proteins are important signaling components of both the canonical β-catenin/Wnt pathway, which controls cell proliferation and patterning, and the planar cell polarity (PCP) pathway, which coordinates cell polarity within a sheet of cells and also directs convergent extension cell (CE) movements that produce narrowing and elongation of the tissue. Three mammalian Dvl genes have been identified and the developmental roles of Dvl1 and Dvl2 were previously determined. Here, we identify the functions of Dvl3 in development and provide evidence of functional redundancy among the three murine Dvls. Dvl3 −/− mice died perinatally with cardiac outflow tract abnormalities, including double outlet right ventricle and persistent truncus arteriosis. These mutants also displayed a misorientated stereocilia in the organ of Corti, a phenotype that was enhanced with the additional loss of a single allele of the PCP component Vangl2/Ltap (LtapLp/+). Although neurulation appeared normal in both Dvl3 −/− and LtapLp/+ mutants, Dvl3 +/−;LtapLp/+ combined mutants displayed incomplete neural tube closure. Importantly, we show that many of the roles of Dvl3 are also shared by Dvl1 and Dvl2. More severe phenotypes were observed in Dvl3 mutants with the deficiency of another Dvl, and increasing Dvl dosage genetically with Dvl transgenes demonstrated the ability of Dvls to compensate for each other to enable normal development. Interestingly, global canonical Wnt signaling appeared largely unaffected in the double Dvl mutants, suggesting that low Dvl levels are sufficient for functional canonical Wnt signals. In summary, we demonstrate that Dvl3 is required for cardiac outflow tract development and describe its importance in the PCP pathway during neurulation and cochlea development. Finally, we establish several developmental processes in which the three Dvls are functionally redundant.
Multi-gene families, comprising a set of very similar genes with shared nucleotide sequences, are common in mammals. Individual family members may be expressed in different places and perform separate functions. Alternatively, the genes may have redundant functions, but distinct dosage requirements. Mammals share three Dishevelled (Dvl) family members and while the roles of Dvl1 and Dvl2 have been described previously, the functions of Dvl3 have remained elusive. Here, we show that the lack of Dvl3 in mice affects the formation of the heart, neural tube, and inner ear. We further show that the defects in these tissues are much more severe when the mice are deficient in more than one Dvl family member, indicating redundant functions for these genes. Congenital heart disease affects approximately 75 in every 1,000 live human births, and approximately 30% of these diseases are due to disruptions in the outflow tract, the region affected in mice lacking Dvl genes. Neural tube defects, similar to those observed in the Dvl mutants, are also common in humans. The animal models described here provide useful tools to elucidate the genetic mechanisms that underlie these abnormalities and may provide novel ways of treating these disorders in the future.
Normal mammalian development is the result of complex signaling networks that regulate and coordinate cell behavior. Wnt signaling controls a broad spectrum of cell fate decisions during embryogenesis and is critical for cell to cell communication in mammalian development. Through the activation of specific target genes, the canonical Wnt pathway tightly regulates cell proliferation, differentiation, adhesion and survival, and controls embryonic patterning ,. A non-canonical Wnt planar cell polarity (PCP) pathway, parallel to that first discovered in flies, has been described in mammals, where it regulates cell polarity and convergent extension (CE) movements. In these coordinated cell movements, cells migrate medially and intercalate, producing an elongation and narrowing of the tissue along the anterior-posterior axis –.
Dishevelled (Dvl) proteins, of which three have been identified in humans and mice – are highly conserved components of both the canonical Wnt and PCP signaling cascades. They function as essential scaffold proteins that interact with diverse proteins, including kinases, phosphatases and adaptor proteins ,. In the canonical Wnt pathway, Dvl transduces the signal activated by Wnt binding to membrane-bound Frizzled (Fz) receptors and low-density lipoprotein-related receptor protein (LRP) 5/6 co-receptors, causing the stabilization and cytosolic accumulation of the critical mediator, β-catenin. Following the translocation of β-catenin to the nucleus, it then binds with members of the T-cell factor (TCF)/lymphocyte enhancer factor (Lef) family of transcription factors to regulate the expression of target genes. Dvl is also one of the core components of the PCP signaling pathway, in addition to Fz, Van Gogh/Strabismus (Vang/Stbm), Flamingo/Starry night (Fmi/Stan), Diego (Dgo) and Prickle (Pk). The specific, highly controlled, asymmetric arrangement of these proteins allows polarity of the cell to be established within the plane of the epithelium and promotes the rearrangement of the cytoskeletal components of the cell.
In the heart, normal development of the cardiac outflow tract requires the addition of cells from the secondary heart field (SHF) dorsal to the primary heart tube ,. SHF cells, which express the LIM-homeodomain transcription factor Islet1 (Isl1), migrate from the pharyngeal mesoderm to contribute to the myocardium of the outflow tract . Recently canonical Wnt signaling has been shown to play a major role in the proliferation and expansion of SHF cells –. It has further been demonstrated that the canonical Wnt signaling mediator β-catenin directly activates Isl1 expression . Extensive remodeling of the outflow tract region with the addition of cardiac neural crest (CNC), mesenchyme from the crest of the neural folds, is then required to septate the single vessel to form the aorta and pulmonary artery . Connections must form between the left ventricle and the aorta, as well as the right ventricle and pulmonary artery, to successfully establish both systemic and pulmonary circulation. Defects in the development of the outflow tract region cause phenotypes such as double outlet right ventricle (DORV), where both the pulmonary artery and the aorta connect to the right ventricle, transposition of the great arteries (TGA) and persistent truncus arteriosis (PTA), where the outflow tract fails to divide into an aorta and pulmonary artery.
Studies of Looptail (LtapLp) mice, which carry a missense mutation in Van Gogh-like 2 (Vangl2, strabismus) ,, a component of the PCP pathway, have also implicated non-canonical Wnt signaling mechanisms in mammalian heart development. LtapLp/LtapLp homozygous mutants display the outflow tract abnormality DORV . Reports indicate that the PCP pathway, which is necessary for the polarized migration of myocardial cells to the outflow tract septum, is disrupted in these mutants ,. Similar outflow tract defects including TGA and PTA have also been observed in mice lacking the non-canonical signals Wnt11  and Wnt5a . Wnt5a has been proposed to function in the outflow tract by stimulating intracellular increases in Ca2+ to regulate cells of the CNC.
PCP signaling is also required for the normal development of the auditory sensory organ, the organ of Corti ,,,. This structure is comprised of one row of inner and three rows of outer sensory hair cells, interdigitated with supporting cells such as inner phalangeal cells, the inner and outer pillar cells and the Deiters cells (reviewed in ). The sensory hair cells have stereociliary bundles uniformly orientated on the apical surfaces, which is the most obvious example of planar cell polarity in mammals. Consistent with this, mouse mutants of core PCP components including Vangl2/Ltap , Fz3 and Fz6 , and Dvl1 and Dvl2  display stereocilia misorientation, indicating that the correct alignment of these stereocilia is dependent on functional PCP signaling . Cellular rearrangements characteristic of CE movements are also required during the development of the organ of Corti ,, during which a thicker and shorter postmitotic primordium undergoes integrated cellular intercalation movements to produce extension along the longitudinal axis and narrowing along a perpendicular axis. Therefore signaling via the PCP pathway is responsible for both the polarized extension and the establishment of planar cell polarity in the organ of Corti.
The neural plate undergoes narrowing and lengthening attributable to CE movements during mammalian neurulation. When PCP signaling is disrupted, cells of the neuroepithelium fail to intercalate, preventing the neural tube from fusing at the midline –. Severe neural tube closure defects are observed in mice carrying mutations in PCP components including Vangl2/Ltap ,, Dvl1 and Dvl2 ,, Celsr (a homolog of flamingo)  and Fz3 and Fz6 . These mutants have shorter and wider neural plates and display craniorachischisis, a completely open neural tube from mid-brain to tail.
To elucidate the role of specific Dvls in mammalian development, we have generated mouse knockouts for each of the Dvl genes. Interestingly distinct phenotypes were revealed, suggesting separate functions for the Dvl proteins. Dvl1 knockout mice are viable and fertile, but display social interaction abnormalities and defects in sensorimotor gating . By contrast, incompletely penetrant cardiac outflow tract abnormalities (50%) and rib/vertebral malformations (>90%) are observed in Dvl2 knockout mice . However, functional redundancy among the Dvl genes is also suggested from their overlapping expression patterns, as well as their high degree of conservation. In support of this, we have previously shown that Dvl1 −/−;Dvl2 −/− mutants display craniorachischisis, a completely open neural tube, and abnormalities in the organ of Corti, both novel phenotypes not observed in the single Dvl knockouts ,.
Here we describe the phenotype of mice deficient in Dvl3 and determine its importance in conotruncal, cochlear and neural tube development. Given the overlapping expression patterns of the Dvls, as well as their high degree of conservation, we further addressed the possibility of functional redundancy and demonstrate that many of the roles of Dvl3 are also shared by Dvl1 and Dvl2. We attribute several of the developmental functions of Dvl3 to its role in PCP signaling, enhancing our knowledge of this essential pathway in mammalian development and further defining the specific role of individual Dvl genes.
Dvl3 knock out mice were successfully generated (Figure S1). In order to calculate the frequency of survival of Dvl3 −/− mice, the genotypes of pups from Dvl3 heterozygote crosses were analyzed at weaning age (Figure 1A). In a 129S6 inbred background, 97 pups were genotyped. No Dvl3 −/− pups survived to weaning, while Dvl3 +/+ and Dvl3 +/− pups were observed in an approximate 12 ratio (34 and 63 respectively), as expected from Mendelian ratios. In a mixed genetic background, 121 pups were analyzed, but only 4 out of the expected 30 Dvl3 −/− mice (13.3%) survived. In contrast, at E18.5 Dvl3 +/+, Dvl3 +/− and Dvl3 −/− genotypes were recovered in normal Mendelian ratios from heterozygous crosses. These data indicate that 100% of Dvl3 homozygotes in a 129S6 inbred background and approximately 87% in a mixed genetic background die shortly after birth. No gross abnormalities were observed in the few adult Dvl3 −/− mice that did survive in a mixed background.
Dvl3 −/− newborn pups had difficulty breathing and were often cyanotic. Examination of hearts from Dvl3 −/− mutants at P0 (Figure 1B) using scanning electron microscopy (Figure 1C) and histochemical analysis (Figure 1D) revealed that all had conotruncal abnormalities. More specifically, seven of the eleven mutant hearts displayed PTA, the outflow tract having failed to divide into an aorta and pulmonary artery, and four showed DORV, where both the pulmonary artery and the aorta connected to the right ventricle.
We generated an EYFP-tagged Dvl3 transgene using homologous recombination of BACs (Figure S2) and observed expression of Dvl3-EYFP in the embryonic heart during conotruncal development at E9.5 (Figure 1E). This transgene fully rescued the lethal defect in Dvl3 −/− mutants, providing formal proof that the phenotype of the Dvl3 −/− mutants is specifically due to the loss of Dvl3. From a cross between a Dvl3 −/− rescued with the Dvl3 transgene and a Dvl3 heterozygote (both in a mixed background), 31% (37/118) of pups genotyped at weaning age were Dvl3 −/− rescued with the transgene, whereas only 1.6% (2/118) Dvl3 −/− mutants survived without the transgene. E18.5 hearts collected from Dvl3 −/− embryos with the Dvl3 transgene appeared normal and displayed no conotruncal abnormalities (Figure 1F and 1G).
Normal development of the outflow tract requires contribution from both the CNC and SHF, so we examined whether a lack of either of these tissues in Dvl3 −/− mutants may be responsible for the observed conotruncal defects by lineage tracing experiments using lineage-specific Cre/LoxP recombination and a Rosa-26-lacZ Cre reporter that expresses β-galactosidase only in cells with Cre activity. Wnt1-Cre, expressed in neural crest cells, was used to label the CNC cell population (Figure 2A–H) and SHF cells were specifically labeled using Isl1-Cre (Figure 2I–P). Embryos were collected and stained at E10.5, E14.5 and E18.5. At each of these stages both CNC and SHF cells were clearly evident in Dvl3 −/− mutants, suggesting that the outflow tract defects were not due to an appreciable loss of either of these lineages.
Neural tube defects were observed in Dvl2 −/− mutants and to a greater extent in Dvl1 −/−;2 −/− double knockouts, indicating functional redundancy . We examined a potential role for Dvl3 in neural tube closure. To determine expression of Dvl3 and Dvl1 in the developing neural tube we used transgenic mice carrying the EYFP-Dvl3 transgene and another, similarly made ECFP-Dvl1 transgene, which rescues the Dvl1 −/−;Dvl2 −/− lethal phenotype (data not shown). Expression of both Dvl3 and Dvl1 was observed in the developing neural tube at E9.5 (Figure 3A–C), an important time for this developmental process. The colors were artificially changed to red and green for Dvl3 and Dvl1, respectively, for easy observation of colocalization (yellow). These Dvls appeared to colocalize in many cells (indicated with white arrows), but not in all cells.
Homozygous LtapLp;LtapLp mutants of the PCP signaling pathway component Vangl2/Ltap, display craniorachischisis, a completely open neural tube from mid-brain to tail ,, whereas no neural tube defects are observed in LtapLp/+ heterozygotes. Although no neural tube defects were observed in any of the Dvl3 −/− mutant embryos collected (data not shown), defective neurulation was apparent when we crossed Dvl3 mutants with LtapLp/+ mice. Several Dvl3 +/−;LtapLp/+ embryos displayed normal neural tube development (Figure 3D–F), whereas others exhibited neural tube abnormalities such as craniorachischisis (Figure 3H–J) and exencephaly, defective closure of the rostral neural tube. Similar phenotypes were observed in Dvl3 −/−;LtapLp/+ mutants, with some appearing normal (Figure 3L–N) and others displaying craniorachischisis (Figure 3P–R). The frequency of these defects was similar in both Dvl3 +/−;LtapLp/+ (7/22, 32%, 5 with craniorachischisis and 2 with exencephaly) and Dvl3 −/−;LtapLp/+ mutants (6/16, 38%, all with craniorachischisis). However, craniorachischisis appears to be a more severe phenotype than exencephaly, indicating a more severe phenotype in Dvl3 −/−;LtapLp/+ mutants compared to Dvl3 +/−;LtapLp/+ mutants.
As both Dvl3 −/− and LtapLp;LtapLp mutants display cardiac defects but the single heterozygotes do not, we looked for defects in the hearts of the Dvl3 +/−;LtapLp/+ double heterozygotes (Figure 3G and 3K). However, all hearts appeared normal, even in the mutants with neural tube defects, while all hearts from Dvl3 −/−;LtapLp/+ double mutant mice (Figure 3O and 3S) displayed conotruncal defects, as expected for Dvl3 −/−mice.
A major role for the mammalian PCP pathway in the development of the organ of Corti has previously been described. Cochleae from mouse mutants homozygous for a mutant allele of the core PCP component Vangl2/Ltap (LtapLp;LtapLp), display misorientation of stereociliary bundles in sensory hair cells due to disrupted planar cell polarity and the cochlear ducts are also shortened and wider due to defects in CE cell movements ,. We have shown that both Dvl1 and Dvl2 function in PCP signaling in the developing cochlea . To assess whether Dvl3 also functions in this process, we examined cochleae from Dvl3 mutant embryos, with and without an extra LtapLp mutation in Vangl2. No cochlear defects were observed in either Dvl3 +/− (data not shown) or LtapLp/+ single heterozygotes in the basal and middle regions (Figs 4A and 4E). However, a mild PCP phenotype was observed in Dvl3 −/− cochleae, where the uniform orientation of stereociliary bundles was disrupted in some hair cells in both the base and the middle of the cochlear ducts (Figure 4B and 4F, respectively). The cochleae of Dvl3 +/−;LtapLp/+ mutants that had normal neural tube development were unaffected. However, in the Dvl3 +/−;LtapLp/+ mutants that showed defective neurulation, a PCP phenotype in the cochlea accompanied the neural tube defect, with the misorientation of several sensory hair cells in both the basal and middle regions (Figure 4C and 4G, respectively). Compared to these two mutants the severity of the phenotype was much increased in Dvl3 −/−;LtapLp/+ mutants that showed neural tube defects, in which many hair cells had disrupted orientation (Figure 4D and 4H). Misorientation of stereociliary bundles was also observed in Dvl3 −/−;LtapLp/+ mutants that had normal neural tube development, but the phenotype was much weaker than in embryos of the same genotype displaying defective neurulation. In the apical regions of the cochlear ducts, rotated stereociliary bundles were observed in the hair cells of LtapLp/+ (Figure 4I), Dvl3 −/− (Figure 4J), Dvl3 +/−;LtapLp/+ (Figure 4K) and Dvl3 −/−;LtapLp/+ (Figure 4L) mutants, although the number of cells affected was increased in both Dvl3 +/−;LtapLp/+ and Dvl3 −/−;LtapLp/+ mutants. In these combined mutants the degree of rotation also appeared more severe, as several stereociliary bundles were completely reversed (Figure 4K).
In addition to rotated stereociliary bundles in the sensory hair cells, strong patterning defects were also observed in Dvl3;LtapLp mutant cochleae. Separately, the patterning in the cochleae of Dvl3 −/− and LtapLp/+ single mutants appeared normal (Figure 4M and 4N). However, the inner ears of Dvl3 +/−;LtapLp/+ and Dvl3 −/−;LtapLp/+ mutants with neural tube defects, were much smaller than in littermate controls (Figure 4O and 4P, respectively). Upon further dissection, the cochleae of the Dvl3 +/−;LtapLp/+ and Dvl3 −/−;LtapLp/+ mutants with craniorachischisis were also much shorter compared to controls (Figure 4M–P). At the cellular level, often in these Dvl3 +/−;LtapLp/+ and Dvl3 −/−;LtapLp/+ mutants the normal arrangement of 3 outer hair cell rows and 1 inner hair cell row was disrupted. In the apical region of Dvl3 +/−;LtapLp/+ and Dvl3 −/−;LtapLp/+ cochleae, additional rows of both outer and inner hair cells were observed (Figure 4K and 4L). Loss of outer hair cell rows was also detected in these mutants, so that the normal three rows became only two rows (Figure 4R and 4S). In total, this patterning defect was observed in 5–20% of the length of the cochlea.
Finally, the location of Dvl3 expression was determined in the inner ear sensory organs, including both the vestibular (Figure 4T) and cochlear (Figure 4U) end organs, using the EYFP-tagged Dvl3 transgene. Dvl3-EYFP signals were asymmetrically localized in cells in all inner ear sensory regions. In the cochlea, Dvl3 was localized either on the lateral sides of the sensory hair cells, or on the medial side of the surrounding supporting cells, similar to what was observed for Dvl2 .
We have previously reported both vertebral and rib malformations in Dvl2 −/−mutants and more severe skeletal defects in Dvl1 −/−;Dvl2 −/− double knockouts . Given the overlapping expression patterns and high homology between the Dvls, we also examined the skeletons of Dvl3 −/− mutants. However, no rib or vertebral abnormalities were observed (data not shown). 7/11 Dvl3 −/− mutants showed xiphoid bifurication, but this was also observed in several wild type controls.
Redundancy between Dvl1 and Dvl2 was evident from our studies of Dvl1 −/−;Dvl2 −/− double mutants, which displayed novel phenotypes not observed in the single Dvl knockouts ,. To address possible redundancy between Dvl3 and the other two Dvls we examined the phenotypes of Dvl1;Dvl3 and Dvl2;Dvl3 double mutants. Both Dvl1 +/−;Dvl3 +/− and Dvl1 −/−;Dvl3 +/− mutants survived to adulthood and were fertile. Normal development was also observed in Dvl1 −/−;Dvl3 −/− embryos until E12.5 (Figure 5A and 5B), but these mutants died soon after of unknown causes, normally between E13.5 and E15.5. Importantly, no neural tube defects were observed.
At mid-gestation Dvl2 +/−;Dvl3 +/− and Dvl2 +/−;Dvl3 −/− double mutants also appear normal (Figure 5D and G), however a striking phenotype was observed in Dvl2 −/−;Dvl3 +/− mutants (Figure 5H). These mutants do not appear to survive beyond E9.5 and display craniorachischisis, pericardial effusion and abnormal looping of the heart, as well as severe posterior truncation. No Dv2 −/−;Dvl3 −/− embryos have been recovered from litters collected from E8.5 onwards, suggesting lethality earlier in development.
Defects are observed in Dvl2 +/−;Dvl3 −/− double mutants at later developmental stages. At E18.5 these mutants appear shorter along the anterior-posterior (A–P) axis, show an abnormal head shape and a truncated snout, as well as a shortened and kinked tail (Figure 5K and L). Craniorachischisis was also observed in two Dvl2 +/−;Dvl3 −/− mutants. One of these with the neural tube defect also showed several other severe phenotypes, including gastroschisis and absence of tail (Figure 5M and 5N).
Since both Dvl2 −/− and Dvl3 −/− mutants both display conotruncal defects, we determined whether these Dvls have redundant or distinct functions in heart development. As common spatial and temporal expression patterns may indicate similar functions, we first confirmed that Dvl3 (Figure 1E) and Dvl2 (Figure 6A) shared comparable expression patterns in the heart at E9.5 during conotruncal development.
We first examined the hearts of Dvl2 +/−;Dvl3 +/− double heterozygotes to see whether these mutants had similar defects as the homozygotes, which would suggest redundant functions for Dvl2 and Dvl3. We observed that Dvl2 +/−;Dvl3 +/− mice often survive to adulthood and are fertile. However, in an inbred background, conotruncal abnormalities were seen in 11/28 Dvl2 +/−;Dvl3 +/− hearts examined at E18.5 (Figure 6B). These defects included 9 DORV, 1 PTA and 1 TGA (Figure 6C–F).
The hearts of Dvl2 +/−;Dvl3 −/− mutants were also examined to determine whether an extra loss of one copy of Dvl2 would worsen the phenotype of Dvl3 −/− mutants. Three Dvl2 +/−;Dvl3 −/− hearts collected at P0 (Figure 6I and 6J) had similar morphology to Dvl3 −/− hearts (Figure 6G and 6H), 1 with DORV and 2 with PTA. However, four Dvl2 +/−;Dvl3 −/− hearts collected appeared to have a worse phenotype than Dvl3 −/− mutants with varying degrees of severity and all displaying PTA (Figure 6K–N). Two were slightly smaller and had lost the characteristic fist shape heart morphology, becoming less tapered towards the bottom (Figure 6K and 6L). The other two Dvl2 +/−;Dvl3 −/− hearts were much reduced in size compared to Dvl3 −/− mutants and had severely altered morphology, with a teardrop shape (Figure 6M and 6N). The embryos that the hearts were collected from were all alive, apart from the two severest phenotypes (Figure 6M and 6N), which were dead. After sectioning, it appeared that both right and left ventricles were still present in all samples.
The generation of fully functional Dvl transgenes (here and in ) allowed us to use a genetic approach to further determine redundancy of function between the Dvl proteins during development. We determined whether an extra copy of either Dvl1 or Dvl2, in the form of Dvl1-ECFP and Dvl2-EGFP BAC transgenes (which we will refer to here as Dvl1TG and Dvl2TG, respectively) was able to rescue the lethal Dvl3 −/− phenotype. Importantly, western blot analysis revealed that none of the Dvl transgenes were overexpressed compared to protein levels from wild type alleles (Figure 7A–C). As shown above, Dvl3 −/− mutants (which still have 2 copies of the Dvl1 allele and 2 copies of the Dvl2 allele) cannot survive. Surprisingly, we found that addition of the Dvl1TG rescued the Dvl3 −/− phenotype such that Dvl3 −/−;Dvl1TG mutants (now Dvl3 −/− with 3 copies of Dvl1 and 2 copies of Dvl2) survived. To test whether this was a complete rescue, we crossed these viable Dvl3 −/−;Dvl1TG mutants to Dvl3 +/− mice and genotyped the progeny at weaning (Figure 7D). Eighteen Dvl3 −/−;Dvl1TG rescued animals were recovered, compared to 16 that would be expected from normal Mendelian ratios if the Dvl1TG is fully able to rescue the Dvl3 −/− phenotype. As these crosses were performed in a mixed background, 4 Dvl3 −/− mice also survived with out an extra copy of another Dvl gene.
Interestingly, in a similar cross using the Dvl2TG, we found that the Dvl3 −/− lethal phenotype was also rescued by an extra copy of Dvl2. We crossed these viable Dvl3 −/−;Dvl2TG mutants with Dvl3 +/− mice and genotyped the offspring at weaning (Figure 7E). Seventeen Dvl3 −/−;Dvl2TG mice were recovered, out of 19 expected from a full rescue, indicating approximately 90% rescue of the Dvl3 −/− lethal phenotype with additional Dvl2.
As 50% of Dvl2 −/− mutants die perinatally, we used a similar strategy to determine whether additional copies of Dvl1 or Dvl3 could rescue Dvl2 −/− lethality. From crosses between Dvl2 −/−;Dvl3TG mutants and Dvl2 +/− mice, 24 Dvl2 −/−;Dvl3TG mutants survived, out of 25 expected from a full rescue, indicating approximately 96% rescue of the Dvl2 −/− phenotype with an extra copy of Dvl3 (Figure 7F). However, from crosses between Dvl2 −/−;Dvl1TG mutants and Dvl2 +/− mice, only 10 Dvl2 −/−;Dvl1TG mutants were recovered, out of 18 expected from Mendelian ratios if the Dvl1TG was able to rescue the Dvl2 −/− phenotype. As 50% of Dvl2 −/− mutants survive without additional Dvl copies, this indicates that adding the Dvl1TG was not able to rescue Dvl2 −/− mutants (Figure 7G).
To confirm that the BAC transgenes were actually behaving as wild type alleles and that ectopic or inappropriate expression levels were not responsible for rescuing the mutant phenotypes, we genetically reduced the Dvl gene dosage by introducing a knock-out Dvl allele and again tested the rescue ability of the transgene. Dvl2 +/−;Dvl3 +/− mutants were crossed with Dvl3 −/−;Dvl2TG mutants and the surviving progeny were genotyped (Figure 7H). Twelve Dvl2 +/+;Dvl3 −/−;Dvl2TG mutants with both wild type alleles of Dvl2 (a total of 3 copies of Dvl2) survived, comparable to 9 expected from normal Mendelian ratios, indicating full rescue. However, no Dvl2 +/−;Dvl3 −/−;Dvl2TG mutants were recovered, even though 9 were expected from normal Mendelian ratios if the Dvl2TG could still rescue. Thus, correction of Dvl2 dosage from three copies to two copies of Dvl2 in Dvl2 +/−;Dvl3 −/−;Dvl2TG mutants (1 wild type allele and the transgene) eliminated the ability of the Dvl2TG to rescue the Dvl3 −/− mutant phenotype, supporting the conclusion that ectopic expression or over-expression of the transgene was not responsible for the rescue of the Dvl3 −/− phenotype.
Similar cochlear defects have been observed in both Dvl1 −/−;Dvl2 −/−  and Dvl3 −/− mutants (Figure 4). Therefore, we examined whether all three Dvls have redundant functions in the developing organ of Corti. The inner ears of double Dvl mutants were studied to determine whether the further loss of an additional Dvl gene would worsen the phenotype observed in Dvl3 −/− mutants. Only the cochlea from Dvl1 −/−;Dvl3 +/−, Dvl2 +/−;Dvl3 +/− and Dvl2 +/−;Dvl3 −/− mutants were examined as other double Dvl mutants were lethal too early in development. The organ of Corti from Dvl1 −/−;Dvl3 +/− mutants appeared normal (data not shown), as did those from the Dvl2 +/− (Figure 8A,F,K) and Dvl3 +/− (not shown) single heterozygote samples. However, several of the sensory hair cells in Dvl2 +/−;Dvl3 +/− double heterozygotes had rotated stereociliary bundles (Figure 8B,G,L). This phenotype appeared worse in the Dvl2 +/−;Dvl3 −/− mutants with the loss of another Dvl3 allele (Figure 8C,H,M) and mild patterning defects were also observed in one of these mutants (Figure 8H). In the Dvl2 +/−;Dvl3 −/− mutants that displayed craniorachischisis, the phenotype was even more severe (Figure 8D,I,N). Throughout the whole cochlea duct the hair cells did not appear to be fully developed, even in the basal region. Additionally, it is hard to see the typical shape of stereociliary bundles in most of the hair cells and the differentiation of these cells is apparently delayed (Figure 8N). Strong patterning defects were also observed as the cochleae of the Dvl2 +/−;Dvl3 −/− mutants were much shorter compared to Dvl3 −/− controls (Figure 8P,Q).
Functional redundancy of the Dvls in cochlea development could only be examined in the Dvl3 −/− mutants rescued with the Dvl2-EGFP transgene. The organ of Corti from Dvl3 −/−;Dvl2TG mutants still displayed misorientation of stereocilia in several of the sensory hair cells (Figure 8E,J,O), similar to the phenotype described for Dvl3 −/− mutants (Figure 4), indicating that increasing the copy number of Dvl2 was not able to restore the correct alignment of stereocilia in the Dvl3 −/− mutants.
Previous studies from our lab , and data presented above are consistent with a role for the Dvls in the PCP pathway during neurulation and cochlear development, while a role for Dvls in Wnt signaling is not as clear. Therefore, BATgal mice, which carry a β-catenin-responsive LacZ gene, were used to determine whether canonical Wnt signaling was functional or interrupted in the Dvl mutants during mid-gestation. Global canonical Wnt signaling patterns appeared largely unaffected in each of the single Dvl mutants compared to their wild type littermates at either E9.5 (Figure 9A–C) or E11.5 (Figure 9D–F). Canonical Wnt signaling was also generally unchanged in the double Dvl mutants Dvl1 −/−;Dvl2 −/−, Dvl1 −/−;Dvl3 −/−, Dv2 +/−;Dvl3 +/−, Dvl2 +/−;Dvl3 −/− and Dvl2 −/−;Dvl3 +/− at E9.5 (Figure 9G–I). However, subtle abnormalities were observed. For example, TCF activity appears reduced in the somites of Dvl1 −/− mutants (Figure 9A) and in the limb bud of Dvl2 −/− mutants (Figure 9B) at E9.5, and the pattern of staining appears altered in both the somites and pharyngeal region of Dvl2 −/− mice at E11.5 (Figure 9E).
A large body of evidence demonstrates that Dvl proteins function in highly conserved pathways in both vertebrates and invertebrates. It is known that in Drosophila ,, Xenopus , –, mouse , and mammalian cell lines –, Dishevelled proteins mediate their effects on the canonical and non-canonical Wnt pathways via highly conserved protein domains. As essential components of both the canonical Wnt and PCP pathways, Dvl proteins are required for many developmental processes. A recent publication has addressed the possibility of redundancy between the three Dvl proteins in mammalian cell lines in vitro . We further aim to determine the action of the Dvl proteins during development in vivo. Having previously described the roles of Dvl1 and Dvl2 in mammalian development ,,, we now describe the specific developmental roles of Dvl3 and additionally use well-established genetic approaches to determine the pathways disrupted in vivo that account for the phenotypes observed in the Dvl mutant mice and to demonstrate redundancy between the Dvls during development in vivo. A summary of the phenotypes of single Dvl and double Dvl mutants is shown in Figure 10.
Dvl3 knockout mice die perinatally with cardiac outflow defects, indicating an important role for Dvl3 in conotruncal development. This is particularly interesting as approximately 30% of all congenital heart diseases are due to defects in this region ,. The loss of Dvl3 causes a similar conotruncal phenotype to those observed with loss of Dvl2 , suggesting redundant functions for these homologous genes in outflow tract development. This was supported both by the similar expression patterns of Dvl2 and Dvl3 found during conotruncal development and the observation of cardiac abnormalities in double Dvl2 +/−;Dvl3 +/− heterozygotes, as compared to normal hearts of Dvl2 +/− and Dvl3 +/− single heterozygotes. The increased severity of the cardiac phenotype of the double Dvl2 −/−;Dvl3 +/− and Dvl2 +/−;Dvl3 −/− mutants, compared with the single Dvl2 −/− and Dvl3 −/− mutants, further indicates redundancy between these Dvls in heart development. Finally, with a genetic approach we found that an extra copy of either Dvl1 or Dvl2, using either the Dvl1-ECFP or Dvl2-EGFP BAC transgene, could rescue lethal Dvl3 −/− heart phenotype, demonstrating the ability of both Dvl1 and Dvl2 to compensate for the loss of Dvl3 to enable normal development.
Our data suggests that Dvl1 shares redundant functions with Dvl2 and Dvl3 in cardiac development. However we found that an additional copy of Dvl1 is not able to rescue the Dvl2 −/− cardiac defects. Although the Dvl proteins appear to have the same expression patterns and redundant functions, we have not yet addressed the possibility of differences in protein expression level. There may a certain threshold of Dvl protein required for normal heart development, but under this threshold there is insufficient signaling to permit normal development. Interestingly, a recent paper by Lee et al. demonstrated that in a number of mammalian cell lines all three Dvls are present, but that the relative levels of expression of the three Dvls differ greatly .
Normal development of the outflow tract requires the addition of cells from both the CNC and the SHF, and a lack of contribution of either of these tissues is often the cause of defects in the conotruncal region. Further, Dvl2 has previously been found to be important for controlling proliferation of CNC cells, via activation of Pitx2 . However, both CNC cells and SHF cells appeared to be present in the absence of Dvl3, suggesting that the heart defects are not due to a complete lack of either of these tissues. Further studies to determine whether these cells are properly situated in relation to the surrounding tissues, and whether they are indeed still functional, are in progress.
Dvl proteins function in both the canonical and non-canonical PCP pathways, therefore disruption of either or both of these pathways could be the cause of the phenotypes observed in Dvl3 −/− mice. Roles for both pathways in heart development have been reported. Canonical Wnt signals appear essential for the proliferation of SHF cells –, whereas the PCP pathway is thought to be necessary for the polarized migration of myocardial cells required in the outflow tract septum (Henderson et al., 2006; Phillips et al., 2005). Dvl3 +/−;LtapLp/+ double heterozygotes displayed no abnormal heart phenotype, suggesting that Dvl3 may function through the canonical and not the PCP pathway during heart development. Conversely, it is possible that the level of PCP signaling was not reduced below the hypothetical threshold required for the phenotype. Further experiments will be required to distinguish these possibilities.
We have shown here that Dvl1 and Dvl3 colocalize in the developing neural tube with similar expression patterns to that of Dvl2 as we described previously , and provide further evidence for a similar functional role for Dvl3 in neurulation as Dvl1 and Dvl2 . Although none of the Dvl single knockouts display neural tube closure defects, disruption of either Dvl1 and Dvl2 or Dvl2 and Dvl3 genes (Dvl1 −/−;Dvl2 −/−, Dvl2 −/−;Dvl3 +/−, Dvl2 +/−;Dvl3 −/−) results in incomplete neurulation, suggesting a dosage sensitive redundant role for all three Dvls in this process. Interestingly, Dvl1 −/−;Dvl3 −/− mutants did not display neural tube defects, suggesting that the three Dvls are not functionally equivalent. Neural tube defects appear in single Dvl mutants when crossed with LtapLp mice, (Dvl2 −/−;LtapLp/+ , Dvl3 +/−;LtapLp/+ and Dvl3 −/−;LtapLp/+), indicating genetic interaction with the Dvls and the PCP component, Vangl2 and therefore that the Dvls signal through the PCP pathway to promote neural tube closure.
It is interesting that both exencephaly and craniorachischisis are observed in Dvl3;LtapLp mutants. Craniorachischisis has been observed in many mutants with disrupted PCP signaling, whereas exencephaly is often associated with defective ciliogenesis (reviewed in ). Recently a connection between cilia and PCP signaling has been suggested as the ciliary proteins Inversin  and Bardet-Biedl Syndrome protein-4 (BBS4)  were shown to influence PCP signaling and CE movements. Additionally, disruption of the PCP effectors inturned and fuzzy in Xenopus laevis demonstrated a link between ciliogenesis, PCP signaling and Hedgehog signaling . Localization of both dishevelled and inturned near the basal apparatus of cilia further suggests a role for PCP components in regulating ciliogenesis .
The polarity defects observed in Dvl3 −/− cochleae were much more severe with the introduction of an additional single LtapLp mutation, indicating that Dvl3 genetically interacts with Vangl2 and functions in the PCP pathway to regulate cell polarity in the organ of Corti. The CE movements driven by the PCP pathway that are required for the extension and thinning of the developing organ of Corti were also disrupted in Dvl3;LtapLp mutants, resulting in shortened organs of Corti relative to controls. Furthermore, the asymmetric localization of Dvl3 during establishment of stereocilia orientation is consistent with the asymmetric localization of Dvl2  and other core PCP components in mammals ,,. The polarized asymmetric arrangement of PCP proteins in the fly wing is also required for the uniform orientation of hairs ,. The sensory hair cells in this region are surrounded by the cellular processes of various types of supporting cells and tight cell contacts are formed between the cells (reviewed in ). Dvl3 appears to be localized on the cellular boundary formed between the sensory hair cells and the projections of the supporting cells, making it difficult to determine whether it is expressed on the lateral side of the hair cell, or on the medial side of the supporting cell. Dvl2 has previously been shown to localize at the lateral side of the hair cell  and as our evidence suggests redundant functions for the Dvl proteins, it seems likely that Dvl3 is also expressed here.
Redundancy between the three Dvls in the developing organ of Corti was implied from the similarity of phenotype in Dvl3 −/− mutants and Dvl1 −/−;Dvl2 −/− double mutants ,. Further, a mild PCP phenotype was observed in Dvl2 +/−;Dvl3 +/− double heterozygotes, despite normal development in the single heterozygotes and the defect in Dvl2 +/−;Dvl3 −/− double mutants was much more severe than in the single Dvl3 −/− mutant. Interestingly, despite redundancy between the Dvls, addition of the Dvl2-EGFP BAC transgene was not able to rescue the rotated stereocilia phenotype in Dvl3 −/− mutants. We again propose that a certain threshold of Dvl protein level may be required for normal development and the relative expression levels of the three Dvls in the developing organ of Corti influences the cochlear phenotype and the ability of a Dvl transgene to rescue this phenotype.
Interestingly, the Dvl3 +/−;LtapLp/+ and Dvl3 −/−;LtapLp/+ mutants that showed the CE/PCP-phenotype in the neural tube also displayed the PCP-phenotype in the organ of Corti. However, the misorientation phenotype was less severe in both Dvl3 +/−;LtapLp/+ and Dvl3 −/−;LtapLp/+ mutants than previously found in LtapLp/LtapLp mutants, in which 95% cells in the two outer hair cell rows are misorientated , possibly due to the remaining Dvl1 and Dvl2 alleles in these mutants. It was also noted that Dvl2 +/−;Dvl3 −/− mutants with defects in the neural tube had a much more severe phenotype in the organ of Corti than those with normal neurulation, indicating a strong correlation between the PCP-phenotypes in these two tissues.
We have previously shown that both Dvl1 and Dvl2 play roles in somite segregation, causing skeletal malformations in mice that lack these genes . Given the high homology between the three Dvl genes, we examined the skeletons of Dvl3 −/− mice. However, no vertebral or rib malformations were observed. Some mutants did display xiphoid bifurication, although we are unsure of the significance of this phenotype as it was also seen in several wild type controls. Severe skeletal defects involving truncation of the A–P axis were, however, observed in Dvl2 −/−;Dvl3 +/− double mutants, supporting previous evidence to suggest redundant roles of the Dvls in somite formation. A similar phenotype of lack of caudal somites and absence of tail bud formation is seen in mice homozygous for a null Wnt3a allele (Wnt3aneo) , and a less severe phenotype appears in the hypomorphic Wnt3a allele mutant, vestigial tail, which shows loss of caudal vertebrae causing a shortening of the tail . Mice lacking Wnt5a are also shortened along the A–P axis with a phenotype similar to Dvl2 +/−;Dvl3 −/− mutants, and share outgrowth defects in the developing face and lack of tail . Wnt3a is classically considered to stimulate canonical Wnt signals, whereas Wnt5a is normally associated with non-canonical Wnt mechanisms, indicating that further investigation is needed to determine which pathway or pathways Dvl is required to signal through for the normal development of these structures. We grossly examined Wnt signaling in both single and double Dvl mutants using the TOPflash reporter of TCF activity. Interestingly we found that global Wnt signaling was largely unaffected in even the most severely affected mutants, suggesting that only a low level of Dvl is sufficient for functional canonical Wnt signals. However, our data also suggests subtle abnormalities in Wnt signaling. Precise determination of these abnormalities will require careful sectioning of specific tissues and examination of various cell types throughout development.
The three highly homologous Dvl proteins shared in mammals have very similar broad expression patterns in development. This study completes the initial characterization of the specific, individual roles of each of these proteins and also establishes functional redundancy and overlap in a number of developmental processes. Dvl3 is required for the development of the cardiac outflow tract and signals in the PCP pathway to regulate CE in the developing neural tube and cochlea, as well as cell polarity in the organ of Corti. Dvl1 and Dvl2 are redundant with each other ,, as well as with Dvl3 in a number of these developmental processes.
All animal care and experiments were performed under protocols approved by the NHGRI/NIH and UCSD Animal Care and Use Committees. LtapLp mutants were originally acquired from Jackson Laboratory, Wnt1-Cre mice were a kind gift of Dr. Andrew McMahon, Harvard and Isl1-Cre mice were a generous gift from Sylvia Evans, UCSD. We generated the Dvl3 −/− and Dvl3-EYFP mouse mutants as described in Figures S1 and S2, respectively. Genotyping of the Dvl3 mutants was performed with the following primers, Dvl3 forward 5′-TCCGATGAGGATGATTCCACC-3′, Dvl3 reverse 5′-TGAGGCACTGCTCTGTTCTGT -3′, Dvl3 knockout 5′-TTGGCCCACAATGGAGATGCCC-3′, NLpgk neo forward 5′-AGGCTTACCCGCTTCCATTGCTCA-3′. The PCR primers used to distinguish between the transgene and wild type Dvl3 allele were Dvl3lnt2 forward 5′-GGACGCAGGAGATCTTTGAA-3′ and Dvl3Int2 reverse 5′-CATAGCTGGGGTTGAAGCTC-3′ which amplify a band of 155 bp for the wild type allele and 189 bp for the transgene, due to the presence of the LoxP site. Genotyping for the following mouse mutants have previously been described; Dvl1 , Dvl2 , Dvl2-EYFP transgene , Wnt1-Cre , Isl1-Cre , Rosa-26-lacZ Cre  and BAT-gal .
After fixing in 3% aldehyde solution (1.5% paraformaldehyde, 1.5% glutaraldehyde) in 0.1 M phosphate buffer pH 7.5, E18.5 hearts were stored in 100% ethanol following dehydration through a graded ethanol series. Hearts were critically point dried, mounted and coated with 300 Angstrom gold-palladium. A Cambridge Instrument Stereoscan 360 scanning electron microscope (Scripps Institute of Oceanography Analytical Facility) was used to view the prepared samples.
P0 hearts were dissected into 10% buffered formalin, dehydrated, embedded in paraffin wax, sectioned at 8 µm thickness and stained with hematoxylin and eosin using standard methods.
To label the CNC and SHF cell populations in Dvl3 −/− hearts, Dvl3 +/− mice carrying Wnt1-Cre  and Isl1-Cre , respectively, were crossed with Dvl3 +/− mice carrying the Rosa-26-lacZ Cre reporter gene . Hearts dissected from embryos collected at E14.5 and E18.5 were stored in 30% sucrose in PBS at 4°C overnight, and then embedded in 7.5% gelatin, 15% sucrose. Sections (20 µm) were cut at −24°C on a cryostat and fixed in 2% formaldehyde, 0.2% glutaraldehyde, 0.02% NP-40 and 0.01% sodium deoxycholate. After washing, the slides were stained with 1 mg/ml X-gal in 35 mM potassium ferrocyanide, 35 mM potassium ferricyanide, 2 mM MgCl2, 0.02% NP-40, 0.01% sodium deoxycholate at 37°C overnight. After re-fixing in 4% paraformaldehyde, the slides were counterstained with Nuclear Fast Red (Vector Lab), according to the manufacturer's protocol. Whole embryos from the above crosses were also collected at E10.5 and stained for β-galactosidase activity using the same procedures. All images were captured using a Spot 2 camera mounted on a Leica DMR light microscope.
To visualize canonical Wnt signaling, Dvl mutant mice were crossed with mice carrying the BAT-gal reporter gene  and embryos collected at various stages of development. Staining for β-galactosidase activity was performed as described above.
To visualize Dvl3 expression, Dvl3-EYFP embryos or cochleae were fixed in 4% paraformaldehyde for 30 minutes or overnight at 4°C. To examine the stereocilia in the sensory hair cells, organs of Corti were stained with fluorescein- rhodamine-conjugated phalloidin (Molecular Probes). Both native YFP signal and fluorescent staining was observed using an Olympus FV 1000 or a Zeiss LSM 510 confocal scanning microscope.
To measure the severity of the patterning defect, the whole cochlea duct was scanned and the cells numbered for length reference. The number of cells in the defective regions (where there were no longer three outer hair cell rows) was then calculated as a percentage of total cells.
Targeted disruption and generation of Dvl3 deficient mice. A Dvl3 genomic clone was isolated from a 129 genomic DNA library in FIX II (Stratagene) as previously described . Dvl3 genomic fragments were subcloned into pBluescript KS II (Stratagene) to enable efficient generation of a Dvl3 knock-out construct (A). A 3.6 kb BamH-Bgl2 fragment from within intron 1 to the middle of exon 2 and a 2.5 kb Dra1-Not1 (from vector MCS) fragment from within intron 7 to beyond 13 were cloned into pPNT  either side of the neomycin resistance gene and in the opposite direction to this marker (A). Gene targeting of this construct in TC1 embryonic stem (ES) cells  was used to generate the knockout mice and individual clones were selected and screened by Dra1 digest of genomic DNA with the 3′ probe. Correct targeting of clone 72 (1 of 100 clones) resulted in the presence of a 7.8 Kb targeted allele and a 6 Kb wildtype allele (B). To confirm homologous recombination, a 5′ probe was used to detect a 5 Kb fragment in Nco1 digested ES clone genomic DNA (B). This correctly targeted clone was injected and successfully transmitted through germline. Lines were established in both mixed (129S6×NIH Black Swiss) and uniform (129S6) genetic backgrounds, as described . F2 litters contained wild type and heterozygous offspring. Dvl3−/− embryos were collected from crosses between heterozygotes and western blot analysis using E13.5 whole embryo lysates confirmed the absence of Dvl3 protein (C). PCR primers (listed in methods) were designed to distinguish wild type, heterozygous and homozygous genotypes (D).
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Generation of EYFP-tagged Dvl3 transgene. An EYFP-tagged Dvl3 transgene was generated using homologous recombination of BACs (A), as described previously . Briefly, BAC clones containing the whole Dvl3 genomic region, including flanking sequences, were identified using overlapping PCR primers covering the entire region from a BAC library (Genome Systems). BAC modifications were performed as previously described , using a SacB-Neo selection cassette (A). An EYFP cassette was fused in-frame to the last codon of Dvl3 and LoxP sites were introduced within intron 2 and the 3′ UTR flanking region. Western blot analysis of transgenic mouse E13.5 embryo lysates was used to confirm expression of the transgene, which was larger than the wild type Dvl3 protein due to the additional EYFP (3B). PCR primers (listed in methods) binding either side of the LoxP site in intron2 (red arrows in A) were used to distinguish between the transgene and wild type Dvl3 allele (C).
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We would like to thank Karl Willert for comments on the manuscript; Andrew McMahon (Harvard) for Wnt1-Cre mice; Sylvia Evans (UCSD) for Islet1-Cre mice; Ella Kothari and Jun Zhao in the UCSD transgenic core for transgenic service and Brendan Brinkman in the UCSD Neuroscience Microscopy Core for help with microscopy.
The authors have declared that no competing interests exist.
This work was supported by grants from the March of Dimes to AWB., the National Institutes of Health to AWB. (HD43173), PC (DC007423), and the UCSD Neuroscience Microscopy Core (NS47101), as well as a postdoctoral fellowship from the American Heart Association to SLE.