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Conceived and designed the experiments: TY JKK HZ OA SM FML. Performed the experiments: TY JKK QL HZ OA LM TC YX SM FML. Analyzed the data: TY JKK QL HZ OA QW KA JR GGF SM FML. Contributed reagents/materials/analysis tools: QW KA JR GGF SM FML. Wrote the paper: TY JKK SM FML.
The p75 neurotrophin receptor (p75NTR) is expressed by neurons particularly vulnerable in Alzheimer's disease (AD). We tested the hypothesis that non-peptide, small molecule p75NTR ligands found to promote survival signaling might prevent Aβ-induced degeneration and synaptic dysfunction. These ligands inhibited Aβ-induced neuritic dystrophy, death of cultured neurons and Aβ-induced death of pyramidal neurons in hippocampal slice cultures. Moreover, ligands inhibited Aβ-induced activation of molecules involved in AD pathology including calpain/cdk5, GSK3β and c-Jun, and tau phosphorylation, and prevented Aβ-induced inactivation of AKT and CREB. Finally, a p75NTR ligand blocked Aβ-induced hippocampal LTP impairment. These studies support an extensive intersection between p75NTR signaling and Aβ pathogenic mechanisms, and introduce a class of specific small molecule ligands with the unique ability to block multiple fundamental AD-related signaling pathways, reverse synaptic impairment and inhibit Aβ-induced neuronal dystrophy and death.
Slowing the progression of Alzheimer's disease (AD) will likely require parallel strategies of managing amyloid-beta (Aβ) levels and reducing neuronal vulnerability to Aβ. With regard to neuroprotective strategies, the p75 neurotrophin receptor (p75NTR) is an attractive target , . Binding of neurotrophins, including nerve growth factor (NGF), to p75NTR promotes pro-apoptotic or pro-survival signaling, depending on the recruitment of survival- versus death-promoting adaptors , . p75NTR is expressed in adult brain primarily by basal forebrain cholinergic neurons, but also by hippocampal, entorhinal and neocortical neurons, each vulnerable in AD (reviewed in ). Moreover, p75NTR expression is upregulated in cortical  and hippocampal  tissue in AD. Increased p75NTR and decreased Trk neurotrophin receptor levels in AD, along with studies showing that increased p75NTR/Trk ratios lead to neuronal degeneration, further encourage therapeutic targeting of p75NTR .
Substantial overlaps exist between p75NTR-mediated signaling and degenerative signaling in AD. In AD brain and in cultured neurons treated with Aβ, there is excessive activation of calpain/cdk5 , GSK-3β , and JNK and its downstream transcriptional activator c-Jun . p75NTR stimulates calpain activation through its Chopper cell death domain,  and mediates NGF-induced inhibition of GSK-3β ; in addition, it can mediate induction of cell death through the activation of JNK . Further, it has been reported that Aβ can bind to p75NTR, and the receptor mediates Aβ-induced cell death, in part by induction of c-Jun activation , , . Thus, there are numerous potential points of direct and indirect interactions between p75NTR and AD pathogenic mechanisms.
p75NTR represents a significant target for AD therapeutic development from a number of perspectives. To the extent that direct interactions between p75NTR and Aβ contribute to AD, preventing those interactions could inhibit neurodegeneration. Further, modulating the receptor to reduce activation of c-Jun and calpain activity could counteract Aβ activation of these signaling intermediates. Moreover, since Aβ down-regulates trophic signaling, particularly the PI3K/AKT pathway which promotes survival and is important for synaptic function , the promotion of AKT activation by ligand binding to p75NTR ,  may reduce the effects of Aβ In addition, AKT down-regulates JNK  and GSK3β . Thus, targeting p75NTR could protect neurons from Aβ by at least three possible mechanisms: i) blocking a deleterious interaction between Aβ and p75NTR; ii) down-regulating deleterious signaling (calpain, GSK3β and c-Jun) which mediates Aβ toxicity; iii) upregulating survival signaling (AKT) which is normally inhibited by Aβ and which can antagonize Aβ mechanisms. The latter two mechanisms could be operative even under circumstances in which Aβ causes degeneration independent of p75NTR.
Aberrant activation of the cdk5, GSK3β and JNK kinases leads to tau hyperphosphorylation, cytoskeletal disruption and neuritic dystrophy . Also, excessive activation of these kinases along with Aβ-induced inhibition of CREB activation causes synaptic dysfunction –. Thus, small molecules inhibiting activation of cdk5, GSK3β, JNK and/or c-Jun have become important therapeutic candidates , , . However, it seems unlikely that modulating a single target will provide an effective therapy; in addition, given the ubiquity of these targets and their functions in a very broad range of cell types it may be anticipated that adverse effects will limit their utility. Therefore, the possibility of inhibiting Aβ-induced excessive activation of cdk5, GSK3β and c-Jun through a receptor expressed by neurons particularly vulnerable in AD is an attractive strategy for therapeutic development.
Expanding on development of synthetic peptides modeled on loop 1 of NGF that prevent neuronal death through p75NTR-mediated mechanisms , we identified small molecule, non-peptide ligands, with favorable pharmaceutical properties, that bind specifically to p75NTR and activate survival-promoting signaling, including AKT, in hippocampal neurons (, reviewed in ). In the present study, we tested the hypothesis that these ligands would interfere with deleterious Aβ signalling and its functional consequences. We demonstrate that these compounds inhibit Aβ-induced neuronal death, neuritic degeneration and activation of calpain/cdk5, GSK3β, and c-Jun; and reverse Aβ-mediated inhibition of AKT and CREB activation, and synaptic function. These findings suggest that the use of small molecule p75NTR ligands may be a therapeutically feasible approach to AD capable of simultaneously targeting multiple underlying pathogenic mechanisms.
Ligands LM11A-24 and LM11A-31 were selected from p75NTR ligands developed by our laboratories based on chemical and pharmacological features favorable for drug development . LM11A-31 is an isoleucine derivative (MW 243.3) and LM11A-24 is a caffeine derivative (MW 322.4). LM11A-36 is structurally similar to LM11A-24 but is inactive in neurotrophic assays  and served as a negative control (Fig. S1). 6–7 DIV hippocampal, cortical and septal neurons were treated with oligomeric Aβ in the presence or absence of p75NTR small molecule ligands or NGF. Preliminary experiments demonstrated that oligomeric Aβ (derived from each of three oligomeric Aβ protocols as described in Methods and Fig. S2) reached maximum toxicity (neuronal death) at 5–10 µM (data not shown), a concentration range similar to that reported by other laboratories. Exposure of neurons to Aβ resulted in somal shrinkage and vacuolation along with neurite fragmentation and beading; whereas the majority of neurons co-treated with Aβ and 100 nM LM11A-24 or LM11A-31 exhibited a normal appearance with intact cell bodies and neurites (Fig. 1A–D). Quantitative analysis of hippocampal, cortical, and septal neuronal cultures demonstrated that Aβ induced a 40–65% reduction in survival while simultaneous addition of LM11A-24 or LM11A-31 prevented 70–90% of the Aβ-induced decrease in survival. NGF and LM11A-36 had no protective effect except for a minor protective effect by NGF in hippocampal and septal cultures (Fig. 1E, G). In dose-response studies, LM11A-24 and -31 inhibited Aβ-induced death with EC50 values of ~20 nM, with the protective effect persisting to at least 500 nM (Fig. 1H, I). LM11A-31 was also found to be protective against Aβ fibrillar preparations (Fig. 1J).
To confirm that p75NTR ligands prevent Aβ-induced neuronal death, hippocampal neuronal survival was further assessed using Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling (TUNEL)/DAPI staining. Neurons treated with Aβ exhibited increased TUNEL staining whereas fewer neurons co-treated with Aβ and LM11A-31 demonstrated TUNEL signal (Fig. 2A–C). Quantitative analyses (Fig. 2D) demonstrated that in the absence of Aβ, addition of LM11A-31 had no significant effect on neuronal survival while NGF was associated with a small but statistically significant increase in death. These findings were consistent with previous studies showing that under certain culture conditions, NGF promotes death of hippocampal neurons . LM11A-31 inhibited Aβ-induced death while NGF had no protective effect. The ability of LM11A-31 and LM11A-24 to block Aβ-induced death was further confirmed using Hoechst staining (Fig. S3).
Previous studies  demonstrated specificity of LM11A-24 and -31 for p75NTR. The ability of p75NTR ligands to induce PI3K/AKT activation was entirely dependent on p75NTR and these ligands did not activate Trk receptors . These previous studies also demonstrated differential activities of neurotrophins and the small molecule ligands on p75NTR-expressing cells. In cultures of oligodendrocytes, which express p75NTR but not Trk receptors, NGF or proNGF can induce cell death; however, LM11A-24 and -31 not only failed to promote death but blocked neurotrophin-induced death. In the current study, we reasoned that if a compound promotes its protective effect through a ligand-type interaction with p75NTR, this effect should be blocked by the addition of a receptor-saturating concentration of a competing, non-protective ligand, such as NGF. The addition of NGF (100 ng/ml) together with LM11A-31 (100 nM) reversed entirely the protective effect of LM11A-31 (Fig. 2D), consistent with a model in which these compounds act through a common receptor. An alternative explanation is that toxicity induced by NGF might mask a non- p75NTR-dependent protective mechanism of LM11A-31. As a second approach for determining whether the LM11A-31 protective effect was mediated through p75NTR, assays were conducted using p75NTR+/+ and p75NTR−/− hippocampal neurons derived from mice maintained on a C57Bl/6-strain background. Using neurons from that strain, maximum Aβ-induced death was reached at 2.5 µM Aβ and this dose was therefore used for subsequent studies. In p75NTR+/+ cultures, Aβ triggered a 2-fold increase in death that was significantly inhibited by LM11A-31, a protective effect consistent with earlier studies (Fig. 2E). In p75NTR−/− cultures, significant Aβ toxicity was reduced to a 1.5-fold increase in death. The significant difference in the degree of cell death triggered in p75NTR wildtype and mutant cultures indicated that the presence of wildtype p75NTR contributes to Aβ-induced toxicity. Notably, in p75NTR−/− cultures LM11A-31 had no protective effect, indicating a protective mechanism requiring p75NTR.
Organotypic slice culture derived from postnatal brain and matured in vitro, is a widely used model for the study of neurodegenerative mechanisms and potential therapeutics. Addition of Aβ to rat postnatal hippocampal slice cultures leads to death of pyramidal neurons as detected by propidium iodide (PI) uptake . In our studies, 24 hour Aβ exposure led to markedly increased in PI staining by pyramidal neurons which was blocked entirely by co-treatment with LM11A-31 (Fig. 3).
Aβ-induced tau/cytoskeletal derangement causes neuritic dystrophy, a process which occurs in early stages of AD  and is characterized by the appearance of varicosities and excessive tortuosity , . Hippocampal neurons kept in vitro for ≥3 weeks express mature isoforms of tau protein, and when exposed to Aβ primarily demonstrate neuritic dystrophy rather than death . Treatment of DIV 21–22 hippocampal neurons with 5 µM Aβ induced dystrophic changes which were prevented almost entirely by LM11A-24 and -31 (Fig. 4A–D). Assessment of dystrophy by visual criteria (Fig. 4D) and by quantitation of the neurite mean differential curvature, a measure of tortuosity (Fig. 4E–H), showed that these ligands effectively blocked Aβ-induced dystrophy. This finding suggests that the compounds may interfere at an early/upstream stage with the complex cascade of Aβ-induced signaling  which results in the disruption of neuritic integrity.
In previous studies with hippocampal neurons, LM11A-24 and -31 were found to activate AKT in a p75NTR-dependent manner and their neurotrophic effect was dependent upon PI3K . These findings raised the possibility that these ligands might counteract the ability of Aβ to decrease levels of AKT activation. Treatment of 21–22 DIV hippocampal neurons with Aβ induced a significant decrease in AKT activity that was blocked by LM11A-24, -31 and NGF, although the effect of NGF was less than that of the small molecules (Fig. 5A). To determine whether the small molecule protective effect was dependent upon PI3K/AKT activity, we co-treated 6–7 DIV hippocampal neurons with Aβ, LM11A-24 or -31, and LY294002, an inhibitor of PI3K. Under baseline conditions (Fig. 5B), LY294002 induced a 1.7-fold increase in cell death without reaching significance, a trend consistent with the known role of PI3K/AKT signaling in promoting neuronal survival . In the presence of Aβ without LY294002, LM11A-24 and -31 blocked the Aβ-death promoting effect consistent with earlier results. However, in the presence of LY294002, the protective effect of LM11A-24 and -31 was significantly reduced. The ability of LY294002 to block the p75NTR ligand protective effect, along with the finding that p75NTR ligands reverse Aβ-induced inhibition of AKT activation, suggest that maintenance of PI3K/AKT signaling by LM11A-24 and -31 contributes to their protective effect.
Activation of calpain results in the cleavage of the 250 kDa cytoskeletal protein α-fodrin to 145 and 150 kDa fragments, and the levels of these fragments serve as a measure of calpain activity . Exposure of 21–22 DIV hippocampal neurons to Aβ triggered a nearly 3-fold increase in the ratio of fragmented to full-length α-fodrin forms (Fig. 5C). Of note, α-fodrin is also a substrate for caspase 3 in degenerative states, with cleavage to 150 kDa and 120 kDa forms . Quantitation of 120 kDa α-fodrin fragments revealed no significant differences across conditions (data not shown) suggesting that Aβ-induced cleavage resulted from calpain rather than caspase activity. LM11A-24, -31 and NGF inhibited Aβ-induced α-fodrin cleavage, indicating that they prevented Aβ-induced calpain activation.
Activated calpain cleaves the cdk5 p35 regulatory subunit to the p25 constitutively active form promoting excessive cdk5 activation; the ratio of p25 to p35 reflects cdk5 activation . Treatment of 21–22 DIV neurons with Aβ resulted in markedly increased p35 cleavage, as evidenced by a significant increase in the ratio of p25 to p35 (Fig. 5D). Co-treatment with LM11A-24, -31 or NGF prevented the increase in p25/p35 ratio, demonstrating that p75NTR ligands inhibit Aβ-induced cdk5 activation.
Given that LM11A-24 and -31 promote PI3K/AKT signaling  and that this signaling inhibits GSK3β  and JNK activation , we determined whether these ligands, as well as NGF, prevent Aβ-induced GSK3β and c-Jun activation. GSK3β activity is increased when the Ser9 residue is dephosphorylated, providing a measure of the GSK3β activation state . Addition of Aβ to 21–22 DIV cultures induced significant dephosphorylation (i.e., activation) of GSK3β, while co-administration with LM11A-24 and -31, but not NGF, prevented this activation (Fig. 5E). An established method for monitoring JNK and c-Jun activation consists of quantitating neuronal nuclei positive for phospho-c-Jun immunostaining . Treatment of 6–7 DIV neurons with Aβ resulted in activation of c-Jun, which was largely inhibited by co-treatment with LM11A-24 or -31 but not by NGF (Fig. 5F).
In summary, LM11A-24 and -31, but not NGF, demonstrated significant inhibition of Aβ-induced GSK3β and c-Jun activation. In contrast, LM11A-24, -31 and NGF each inhibited the ability of Aβ to downregulate AKT signaling and to promote calpain/cdk5 signaling. These differences in signaling profiles between the small molecules and NGF might account, in part, for the greater protective effects of the small molecules and point to the importance of GSK3β and c-Jun activation in mediating Aβ-induced degeneration.
Since cdk5, GSK3β and JNK contribute to Aβ-induced tau phosphorylation , , , we determined whether LM11A-24 and -31 might also prevent tau phosphorylation. In 21–22 DIV neurons, exposure to Aβ resulted in a significant increase in tau phosphorylation at Ser202, a well characterized tau residue which is phosphorylated by each of these kinases and which is found to be phosphorylated in early AD , , . Co-administration of LM11A-24 or -31 almost entirely prevented Aβ-induced tau Ser202 phosphorylation (Fig. 5G).
In addition to aberrant activation of calpain/cdk5, GSK3β and JNK, another potential mechanism by which Aβ inhibits synaptic function is through inhibition of CREB, a fundamental contributor to long-term potentiation (LTP) . To further examine the effects of LM11A-24 and -31 on Aβ-induced changes in signaling, we determined whether these ligands were capable of blocking Aβ-induced CREB deactivation. Treatment of 21–22 DIV hippocampal neurons with Aβ for 3 hours resulted in a 43% reduction in phosphorylation of CREB, consistent with previous studies . Notably, co-treatment with 100 nM LM11A-24 or -31 blocked the inhibitory effect of Aβ on CREB phosphorylation (Fig. 5H).
The effect of the ligands on CREB phosphorylation strongly suggested that they might be able to reverse the Aβ-induced inhibition of LTP in the brain. Electrophysiological experiments were performed on hippocampal slices derived from adult mice that received a tetanus to produce LTP at the Shaffer collateral-CA1 connection. As previously shown , we found that the exposure of slices to Aβ (200 nM) for 20 min before tetanization led to significant impairment of LTP (Fig. 6). However, when slices were treated concomitantly with Aβ and LM11A-31 (100 nM), LTP was normalized. LM11A-31 alone did not affect potentiation.
There is no available therapy for AD that effectively targets underlying disease mechanisms. The present studies demonstrate that non-peptide small molecule ligands targeting p75NTR, a receptor upregulated in neurons vulnerable in AD, prevent Aβ-induced neuritic dystrophy and cell death, while inhibiting Aβ-induced tau phosphorylation and the activation of several key signaling intermediates, each a candidate therapeutic target in its own right. The inhibition of Aβ-induced and transgenic mouse-related synaptic impairment, the prevention of neuritic dystrophy and neuronal death, along with the modulation of multiple intracellular signaling mechanisms involved in AD is a novel activity profile for small molecule ligands acting at a known receptor target.
The protective effect of these compounds is likely mediated through p75NTR. Their effects on neuronal signaling and survival are p75NTR-dependent, they induce signaling-adaptor recruitment to p75NTR and they fail to activate Trk receptors . In addition, a standardized receptor binding screen conducted with LM11A-31 (Cerep panel, Table S1) failed to detect binding of p75NTR ligands to other receptors. NGF lacked protective activity, and as expected for an ‘inactive’ ligand, inhibited the protective effect of p75NTR small molecule ligands, consistent with their protective effect being mediated through p75NTR. Moreover, in assays employing p75NTR−/− neurons in which Aβ-induced death was reduced but still present to a significant degree, small molecule ligand protective activity was entirely absent. Taken together, our prior and current studies indicate that these ligands inhibit Aβ-induced degeneration by interacting with p75NTR. The expression of p75NTR by glial cells  raises the possibility that these ligands might also function through p75NTR-mediated effects on non-neurons; however, the relative lack of non-neuronal cells in the culture protocols applied here and the effects evident in short term signaling studies make this possibility unlikely.
There are several mechanisms by which p75NTR ligands might inhibit Aβ toxicity. Aβ(1-40) aggregates bind to p75NTR and induce cell death and a modified NGF loop 1 synthetic peptide was found to block NGF binding to p75NTR and prevent Aβ(1-40)-induced death of cultured cortical neurons . It has yet to be determined whether Aβ(1-42) oligomer species similarly bind to p75NTR to induce neuronal degeneration. Our finding that Aβ(1-42) oligomer-induced death is significantly, though incompletely, decreased in p75NTR−/− cultures suggests that p75NTR might be one of several targets mediating oligomeric Aβ(1-42) effects. Thus, small molecule p75NTR ligands might prevent degeneration, in part, by preventing Aβ binding to p75NTR. Alternatively, p75NTR might not be an important target for Aβ binding but might instead contribute to Aβ toxic effects through indirect mechanisms. Its ability to affect deleterious or survival-promoting signaling (i.e. c-Jun and PI3K/AKT, respectively) might contribute an ‘enabling’ mechanism that augments Aβ toxicity. In this scenario, the protective function of small molecule ligands would be derived from optimizing the degenerative- versus survival-promoting functions of p75NTR rather than inhibiting Aβ oligomer binding. The findings that NGF has no protective effect against Aβ in some assays, and that the toxic effect of Aβ is only partially lost in p75NTR−/− neurons, suggest that the protective effect of p75NTR small molecule ligands, especially in assays in which toxic effects of Aβ are blocked entirely, is unlikely to be mediated solely by a simple mechanism of inhibiting Aβ binding to p75NTR.
Deleterious functional effects promoted by nanomolar, non-lethal, concentrations of Aβ may occur through mechanisms in addition to those promoting gross neuronal dystrophy and death. Recent findings suggest that p75NTR is highly enriched in post-synaptic densities and interacts with the PDZ3 domain of the PSD-95 scaffolding protein . In addition, evidence continues to accumulate that Aβ might impair synaptic function by perturbing PSD-95 related structures and function , . Thus, the finding that LM11A-31 is capable of blocking low concentration Aβ-induced impairment of synaptic function in adult hippocampal slices suggests additional pathways through which p75NTR small molecule ligands may mitigate Aβ effects. LM11A-31 is the first small molecule ligand shown to prevent Aβ-induced synaptic LTP impairment when administered at nanomolar, rather than micromolar, concentrations.
The favorable drug development profile of LM11A-24 and -31, their low nanomolar potency and receptor selectivity, and their unprecedented ability to simultaneously inhibit Aβ-induced activation of calpain/cdk5, GSK3β and c-Jun and to block Aβ impairment of synaptic function, each of which are current pharmaceutical candidate targets for AD therapeutics, establish an important new class of candidate small molecule compounds for AD therapeutics. Novel derivatives of these compounds with optimized pharmaceutical profiles are currently under study and will serve as important candidates for clinical development. Current studies in our laboratories are assessing the ability of p75NTR ligands to decrease neurite degeneration and to correct behavioral deficits in transgenic mice that over-express Aβ.
All chemicals were purchased from Sigma (St Louis, MO), unless otherwise stated. The PI3-kinase inhibitor LY294002 was obtained from Calbiochem (La Jolla, CA). Anti-phospho-GSK3βSer9 polyclonal antibody, anti-phospho-AKT monoclonal antibody, anti-phospho-CREB polyclonal antibody, anti-monoclonal CREB antibody, anti-AKT polyclonal antibody and anti-phospho-c-Jun polyclonal antibody were purchased from Cell Signaling (Beverly, MA). Monoclonal anti-GSK3β was obtained from BIOSOURCE International (Camarillo, CA). Monoclonal anti-phospho-tauSer202 was purchased from Pierce (Rockford, IL). Polyclonal anti-tau, monoclonal microtubulin-associated protein-2 (MAP2) and monoclonal anti-actin antibodies were obtained from Sigma. α-fodrin and p35/p25 antibodies were procured from Santa Cruz Biotechnology (Santa, Cruz, CA). Mouse submaxillary NGF was obtained from Invitrogen (San Diego, CA).
Animal procedures were approved by each participating university's Committee on Laboratory Animal Care and were conducted in accordance with the NIH Guide for the care and use of laboratory animals. Cortical, hippocampal and septal cultures were prepared from embryonic day 16 (E16) CF1 mouse fetuses . Tissue culture wells with or without coverslips were coated with 10 µg/mL poly-L-lysine in PBS. Cells were incubated in DMEM/F12 containing 10% fetal bovine serum for the first 16-20 hours, and subsequently maintained in serum-free Neurobasal medium with 1× B27 supplement (Invitrogen). p75NTR+/+ or −/− hippocampal neurons were derived from E15-17 mice maintained on a C57Bl/6 background and cultured under the same conditions, with the addition of 1 mM Glutamax supplement. For neuronal viability assays, neurons were seeded in 24-well plates at a density of 20,000–30,000 cells per well or in 12-well plates at 80,000–100,000 cells per well and allowed to mature 6–7 days. For neuritic dystrophy assays, 150,000–200,000 neurons per well were seeded into 6-well plates containing 25 mm coverslips and matured 21–22 days. For all cultures, medium was changed every 48–72 hours, prior to the addition of Aβ and various compounds. MAP2 immunostaining demonstrated that each of the above protocols resulted in cultures containing 90–95% neurons with 100% of the MAP2-positive cells expressing p75NTR. For Western blot signaling assays, the same protocol was followed except neurons were seeded at 450,000 cells per well in 6-well plates.
Aβ(1-42), referred to as “Aβ”, and Aβ(S) (Aβ(1-42) residues in a scrambled sequence) were obtained from rPeptide (Athens, GA). Aβ oligomers were prepared using three different established methods. For the first two, Aβ peptide was resuspended in 0.5 mM NaOH  or 0.2% NH4OH  at a concentration of 350 µM and stored at −70°C. For use in cell cultures, the stock solution was incubated at 37°C for 5–7 days. In the third method , 1.0 mg of Aβ peptide was dissolved in 250 µl hexafluoroisopropanol (HFIP), aliquoted in sterile microcentrifuge tubes and HFIP was removed under vacuum in a Speed Vac. Resulting peptide films were stored desiccated at −20°C. Before use, the peptide was resuspended to 5 mM in dry dimethyl sulfoxide (Me2SO, Sigma), brought to 80 µM in PBS and incubated at 4°C for 16–24 hours. For the production of Aβ fibrils, HFIP-prepared peptide was brought to 222 µM in 10 mM HCl.
Peptide preparations were characterized by Atomic Force Microscopy (AFM). A multimode scanning probe microscope controlled by a NanoScope IIIa controller was used in conjunction with an E-series piezoelectric scanner (Digital Instruments, Santa Barbara, CA). AFM probes were etched silicon micro cantilevers, model MPP-11100 (Veeco, Santa Barbara, CA). Samples were applied to freshly cleaved mica using a Langmuir-Schaffer horizontal transfer system, and were then rinsed with Milli-Q Ultrapure water (Millipore, Temecula, CA) . Image data was acquired at a scan rate of 0.5–1 Hz. AFM analysis (Fig. S2) of Aβ preparations demonstrated that NaOH- and NH4OH-based protocols resulted in primarily oligomeric species with occasional fibrils and that HFIP-based protocols resulted in primarily oligomeric species with rare or absent fibrils. The HCl-based protocol yielded primarily fibrillar species. Oligomeric Aβ preparations were used in all studies except as indicated in Fig. 1J.
p75NTR ligands (LM11A-24 and LM11A-31), NGF or signaling inhibitors were added concomitantly with Aβ. Aβ preparations were added to 6–7 DIV cultures at a final concentration of 5 or 10 µM followed by 72 hours incubation. Following incubation with Aβ neurons were stained with Syto 13 (Molecular Probes), Hoechst 33258 (Calbiochem); or TUNEL/DAPI, using the fluorescein-12-dUTP, DeadEnd™ Fluorometric TUNEL System (Promega Madison, WI), and VECTASHIELD®+DAPI (Vector Labs Burlingame, CA). Stained neurons were visualized under a fluorescence microscope (Leica DM IRE2) using 520 nm (TUNEL, Syto13) or 460 nm (DAPI, Hoechst) filters. Survival of neurons was determined based on morphological criteria and Syto-13 (aids in cellular visualization) as assessed by phase contrast microscopy . A dead or degenerating neuron was defined as one with a vacuolated cytoplasm, shrunken soma and/or beaded or retracted neurites. Data are expressed as percent of the total number of observed neurons that were scored as surviving. Neuron death was quantified with Hoechst 33258 by counting the number of cells containing condensed or fragmented nuclei as a percent of total observed neurons, and also with the TUNEL/DAPI system, by dividing the number of nuclei exhibiting TUNEL staining by the total number of nuclei as identified by DAPI. Cell survival analyses were confirmed by blinded counts.
21–22 DIV hippocampal neuron cultures were treated with fresh Neurobasal B27 medium containing Aβ at a final concentration of 5 µM in the presence or absence of LM11A-24 or -31 for 48 hours and then fixed in fresh 4% paraformaldehyde. Neurites were imaged by immunostaining with MAP-2 monoclonal primary followed by Cy3-conjugated anti-mouse secondary antibody (Jackson Immunoresearch). MAP2 positive dendrites were quantitated using the MEASURE COUNT OBJECT function of MetaMorph 5.0 (Universal Imaging Inc, West Chester, PA). Only dendrite branches longer than 10 µm were considered (processes shorter than 10 µm were considered as sprouts). Dendrites were considered dystrophic when they showed a persistent pattern of increased tortuosity (multiple abrupt turns). To more precisely quantitate the degree of neurite curvature, we modified an established method for assessment of neurite curvature . In randomly selected fields, neurite courses were digitized and approximated by a series of n manually chosen connected line segments using NIH-Image (Fig. S4). Neurite analyses were confirmed in a blinded manner. Using a Sigmaplot macro, the angle of each segment was determined relative to a line connecting the endpoints of the neurite tracing (a), and, beginning at one end, successive angles were subtracted from the prior angle in the chain, and the results averaged to give the ‘mean differential curvature’ (MDC=Σ(ai+1−ai)/n). This parameter reflects the degree of curvature over the course of the neurite with an increasing value indicating increased curvature.
Organotypic hippocampal slice cultures were prepared using previously described methods  with modification. Briefly, 350 µm thick hippocampal slices were prepared from postnatal 8-day-old (PND-8) Wistar rats using a tissue chopper and separated in ice-cold Hank's balanced salt solution (HBSS) with 33.3 mM glucose, 4.2 mM NaHPO4, 10 mM MgSO4, 10 mM HEPES, 0.3% BSA and Penicillin-Streptomycin, pH 7.3. Slices were mounted on Millicell culture inserts and transferred to 6-well culture plates. Each well contained 1 mL of tissue culture medium consisting of 50% minimum essential medium, 25% HBSS, 25% heat inactivated horse serum and supplemented with 33 mM glucose, 12.5 mM HEPES and penicillin-streptomycin. Prior to the addition of Aβ and small molecules, organotypic cultures were matured under tissue culture conditions for 11–19 days. Culture medium was changed three times per week.
Pyramidal neuron death in brain slices was quantitated by measuring propidium iodide (PI) uptake using a previously established protocol . In brief, slices were treated with Aβ±LM11A-31 for 24 hours and then with 50 µM N-Methyl-D-Aspartic acid (NMDA) to induce maximum pyramidal neuron death in each slice. Cellular damage was assessed by fluorescent image analysis of propidium iodide uptake (PI; 2 µg/mL), indicative of significant membrane injury. Before and after treatment, slices were observed and photographed with an inverted microscope (Nikon) coupled to a CCD camera, and mean fluorescent intensity in the CA1-3 regions was determined using OpenLab 4.04 software. Baseline (i.e. pre-treatment) fluorescence was subtracted from subsequent measurements and post-treatment cellular damage was normalized to the total neuronal density (determined by NMDA-induced PI intensity).
For assays of AKT, calpain, cdk5 and GSK3β activity, 21–22 DIV hippocampal neurons were incubated for 4 hours in fresh neurobasal/B27 culture medium containing 5 µM oligomeric Aβ in the presence or absence of LM11A-24, -31 or NGF. Following incubation, cells were collected and washed in ice-cold PBS and then lysed in RIPA buffer (20 mM Tris, pH 8.0, 137 mM NaCl, 1% NP-40, 10% glycerol, 1 mM PMSF, 500 µM orthovanadate, 10 µg/ml aprotinin and 1 µg/ml leupeptin). Lysates were sonicated for 10 seconds, centrifuged at 14,000×g for 10 minutes at 4°C, and the supernatant was collected. For tau phosphorylation studies, heat-stable fractions were prepared as described previously . In brief, cells were collected by scraping cells into ice-cold TBS and centrifuging at 14000×g for 10 min at 4°C. The supernatant was discarded and the pellet was resuspended in 100 µl MES/NaCl buffer (100 mM MES, 1 M NaCl, 0.5 mM MgCl2, 1 mM EGTA, 2 mM DTT, 1 mM Na3VO4, 1 mM benzamidine hydrochloride, 5 µg/ml leupeptin, 2 µg/ml aprotinin, 1 µg/ml pepstatin, 0.2 mM PMSF) and immediately heated to 100°C for 10 min. These were then cooled on ice and centrifuged at 14000×g at 4°C for 25 min. The tau and MAP2c-enriched supernatant was retained. Phosphorylated CREB (p-CREB) levels were determined as described previously . Cultured 21–22 DIV hippocampal neurons were treated with 5 µM Aβ in Neurobasal medium without B27 supplement or L-glutamine for 3 hours followed by the addition of 50 µM glutamine for 15 min in order to promote CREB activation (phosphorylation). Cells were harvested in 1× modified RIPA buffer as above with the addition of 80 mM glycerophosphate, and whole-cell extracts were then prepared. Protein concentrations were determined using the BCA Protein Assay Reagent (Pierce, Rockford, IL). Samples were electrophoresed through 4–20% Tris-HCl Linear Gradient Gels (Bio-Rad, Hercules, CA) and transferred to PVDF membranes. Western blots were processed using the ECL Chemiluminescence System (Amersham, Arlington Heights, IL) and bands were quantitated using densitometry.
E16 hippocampal neurons harvested from CF-1 mice were plated on glass coverslips as described above or on Tissue Tek chamber slides and at 6–7 DIV were treated with 5–10 µM oligomeric Aβ for 12 hours, then fixed in fresh 4% PFA and immunostained with phospho-c-Jun specific antibody and DAPI or Hoechst to label total nuclei . Activation of c-Jun was quantitated by counting p-c-Jun positive nuclei as a percent of total neuronal nuclei.
Hippocampal slice preparations were performed as described . Hippocampi were harvested from 3 month old male mice (C57Bl/6; The Jackson Laboratory). Transverse hippocampal slices (400 µm in thickness) were cut and maintained in an interface chamber at 29°C, and perfused with saline solution (124.0 mM NaCL, 4.4 mM KCL, 1.0 mM Na2HPO4, 25.0 mM NaHCO3, 2.0 mM CaCL2, 2.0 mM MgSO4, and 10 mM glucose) continuously bubbled with 95% O2 and 5% CO2. fEPSPs were recorded from the CA1 region of the hippocampus by placement of both the stimulating and the recording electrodes in the CA1 stratum radiatum. Basal synaptic transmission (BST) was assayed by plotting of the stimulus voltage (V) against slopes of fEPSP to generate input-output relations or by plotting of the peak amplitude of the fiber volley against the slope of the fEPSP to generate input-output relations. LTP was induced using theta-burst stimulation (4 pulses at 100 Hz, with the bursts repeated at 5 Hz, and each tetanus including 3 10-burst trains separated by 15 seconds). LM11A-31 (100 nM) (dissolved in H2O) or vehicle (H2O) was added to the bath solution for 20 minutes prior to the induction of LTP in studies in which Aβ (200 nM) was added to slice preparations. Oligomeric Aβ(1-42) was prepared using HFIP .
Statistical analyses applied ANOVA with Dunnett's correction except for the following: in Fig. 2 and Fig. 5B ANOVA with Tukey-Kramer correction was applied; and in Fig. 6 two-way ANOVA was applied. For all bar graphs, mean±SE is shown. For all figures: *p<0.05, **p<0.01, ***p<0.001.
Small molecule structures. Structures of LM11A-31, LM11A-24 and LM11A-36 are shown. LM11A-36 is identical to LM11A-24 except that it contains two additional methyl groups and is inactive. These compound structures were published previously .
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AFM imaging of Aβ preparations. Images are representative ~1×1 µm scans with z-height 10 nm. Incubation Aβ(1-42) peptide in NaOH (A) or NH4OH (B) demonstrates primarily oligomeric structures with occasional fibrillar structures. (C) Aβ preparation derived from HFIP-processing of Aβ followed by PBS incubation demonstrates oligomeric structure with no observed fibrils. (D) Aβ fibril preparation derived from HFIP-processing of Aβ followed by HCl incubation demonstrates primarily fibril-like structures.
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LM11A-24 and -31 inhibit Aβ-induced death of hippocampal neurons as assayed by Hoechst staining. 6–7 DIV hippocampal neurons were treated with (A) culture medium (CM) alone; (B) 10 µM Aβ; (C) 10 µM Aβ with 100 nM LM11A-24; (D) 10 µM Aβ with 100 nM LM11A-31 for 72 hours, then stained with Hoechst 33258, fixed and photographed with fluorescence microscopy. Many neurons exposed to Aβ exhibited nuclear condensation and fragmentation, indicative of death (arrows in B), whereas the majority of neurons co-treated with Aβ and LM11A-24 or -31 had diffuse, even nuclei, similar to the control condition. (E) Treatment with Aβ resulted in an approximately 4-fold increase in neuronal death. Co-treatment with 100 nM LM11A-24 or -31, but not NGF, prevented Aβ-induced death (n=18–58 fields derived from 3–6 separate experiments). Each condition was compared to Aβ alone.
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Illustration of neurite curvature quantitation. As described in Methods, neurites were traced manually in the form of a series of short vectors (small arrows along neurites) to determine the angles (ai, ai+1…) created by each vector and a line connecting the origin and termination of the measured segment. The differences between successive angles (e.g. ai+1-ai) were averaged to generate a mean differential curvature (MDC) score. The upper panel demonstrates a neurite in culture medium alone (CM) with a mean differential curvature of 10.7 and the lower panel demonstrates a neurite exposed to Aβ with a mean differential curvature of 29.3.
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Cerep receptor screen. Compound LM11A-31 was submitted to Cerep Inc. (Seattle, WA) and applied to the ExpresSProfile receptor screen. Values shown indicate the percent by which binding of a control ligand is inhibited by the test compound. Inhibition of binding by <20% is interpreted as no significant binding detected.
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We thank Rick Meeker, Mohanish Deshmukh and Martin Widenbrandt for helpful discussions and technical advice.
Competing Interests: Dr. Longo is a founder of PharmatrophiX Inc., a company focused on the development of neurotrophin receptor ligands.
Funding: This project was supported by the Institute for the Study of Aging (FL), Alzheimer's Association (FL), the Eastern Chapter of the North Carolina Alzheimer's Association (FL), NINDS F30 NA051971 (JK), NIA AG026630 (QL), NCI CA111891 (QL), NS049442 (OA) and the Veterans Administration (SM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.