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Figure 7.

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Override of taxol-induced mitotic arrest by I-2 knockdown or hesperadin treatment. ARPE-19 cells were transfected for 48 h with siRNA against I-2 or luciferase as control, and then they were incubated with 5 μM taxol in the presence of siRNA for another 12 h, before fixation and staining with Hoechst 33342. For hesperadin treatment, cells were treated with 100 or 500 nM hesperadin for 2 h, incubated with 5 μM taxol in the presence of hesperadin for another 12 h, and then fixed and stained with Hoechst 33342 to identify the percentage of mitotic cells (500 cells/group). Data are means ± SD of values from three independent experiments.

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