Localization of I-2 during mitosis in ARPE-19 cells. (A) ARPE-19 cells expressing HA-I-2 at low levels were fixed and stained with anti-HA (orange), anti-α-tubulin (green) antibody, and Hoechst 33342 (blue). Cells at metaphase (top), early anaphase (middle), and late anaphase (bottom) are shown. (B) Localization of endogenous I-2 in ARPE-19 cells at metaphase (top), early anaphase (middle), and late anaphase (bottom) that were fixed and stained with Hoechst 33342 (blue) and sheep anti-I-2 antibody (orange). Bar, 20 μm.
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