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Mol Cell Biol. Nov 2008; 28(22): 6773–6784.
Published online Sep 15, 2008. doi:  10.1128/MCB.00941-08
PMCID: PMC2573297
MicroRNA-155 Is Regulated by the Transforming Growth Factor β/Smad Pathway and Contributes to Epithelial Cell Plasticity by Targeting RhoA[down-pointing small open triangle]
William Kong,1,2 Hua Yang,1 Lili He,1 Jian-jun Zhao,1 Domenico Coppola,3 William S. Dalton,4 and Jin Q. Cheng1*
Departments of Molecular Oncology,1 Pathology,3 Experimental Therapeutics, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612,4 Department of Pathology and Cell Biology, College of Medicine, University of South Florida, Tampa, Florida 336122
*Corresponding author. Mailing address: H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Dr., SRB3, Tampa, FL 33612. Phone: (813) 745-6915. Fax: (813) 745-3829. E-mail: jin.cheng/at/moffitt.org
Received June 12, 2008; Revised August 1, 2008; Accepted September 8, 2008.
Transforming growth factor β (TGF-β) signaling facilitates metastasis in advanced malignancy. While a number of protein-encoding genes are known to be involved in this process, information on the role of microRNAs (miRNAs) in TGF-β-induced cell migration and invasion is still limited. By hybridizing a 515-miRNA oligonucleotide-based microarray library, a total of 28 miRNAs were found to be significantly deregulated in TGF-β-treated normal murine mammary gland (NMuMG) epithelial cells but not Smad4 knockdown NMuMG cells. Among upregulated miRNAs, miR-155 was the most significantly elevated miRNA. TGF-β induces miR-155 expression and promoter activity through Smad4. The knockdown of miR-155 suppressed TGF-β-induced epithelial-mesenchymal transition (EMT) and tight junction dissolution, as well as cell migration and invasion. Further, the ectopic expression of miR-155 reduced RhoA protein and disrupted tight junction formation. Reintroducing RhoA cDNA without the 3′ untranslated region largely reversed the phenotype induced by miR-155 and TGF-β. In addition, elevated levels of miR-155 were frequently detected in invasive breast cancer tissues. These data suggest that miR-155 may play an important role in TGF-β-induced EMT and cell migration and invasion by targeting RhoA and indicate that it is a potential therapeutic target for breast cancer intervention.
Metastasis accounts for the majority of deaths of cancer patients, and thus, it is crucial to understand the molecular and cellular mechanisms that cause primary tumors to metastasize. The most critical step in the conversion of primary tumors to metastases is attributed to the process known as epithelial-mesenchymal transition (EMT). EMT is a remarkable example of cellular plasticity that involves the dissolution of epithelial tight junctions, the intonation of adherens junctions, the remodeling of the cytoskeleton, and the loss of apical-basal polarity (49, 55). In cells undergoing EMT, the loss of epithelial cell adhesion and cytoskeletal components is coordinated with a gain of mesenchymal components and the initiation of a migratory phenotype.
Transforming growth factor β (TGF-β) has emerged as a key regulator of EMT in late-stage carcinomas, where it promotes invasion and metastasis (54). TGF-β binds to a heteromeric complex of transmembrane serine/threonine kinases, the type I and II TGF-β receptors (TβRI and TβRII). Following ligand binding to TβRII, the type I receptor is recruited to the ligand-receptor complex, where the constitutively active TβRII transactivates TβRI. Activated TβRI phosphorylates the receptor-specific Smad2 and Smad3. Phosphorylated Smad2/Smad3 associates with Smad4 as a heteromeric complex and translocates to the nucleus. This complex binds directly to Smad-binding elements and associates with a plethora of transcription factors, coactivators or corepressors, thus leading to the transcriptional induction or repression of a diverse array of genes (54). A number of genes that are associated with tumor growth and metastasis have been shown previously to be directly regulated by this pathway, the effects of which include the induction of COX2, Slug, Snail, and Twist and the repression of Id2 and Id3 (54). Recent reports have shown the importance of microRNA-200 (miR-200) family downregulation during EMT (2, 12, 21, 36); however, the functions of upregulated miRNAs during TGF-β-induced EMT remain uncharacterized.
miRNAs are a class of 22-nucleotide noncoding RNAs that are evolutionarily conserved and function as negative regulators of gene expression. Like conventional protein-encoding mRNA, miRNAs are transcribed by RNA polymerase II and controlled by transcription factors (1, 9, 16, 38). The primary transcript (pri-miRNA) is capped and polyadenylated. The pri-miRNA is processed by the nuclear RNase III Drosha and its cofactor DGCR8/Pasha to generate a precursor miRNA, a 60- to 70-nucleotide RNA that has a stem-loop structure (3, 13, 15). The precursor miRNA is rapidly exported to the cytoplasm by exportin-5 in a Ran-GTP-dependent manner, where it is further processed by a second RNase III, Dicer, to release a mature ~22-nucleotide miRNA. Subsequently, the mature miRNA enters an RNA-induced silencing complex, guides this complex to regions of complementarity in the 3′ untranslated region (UTR) of target mRNAs, and triggers either their degradation or the inhibition of translation, depending on the degree of complementarity between the miRNA and its target mRNA (24, 44). Based on predictions by publicly available algorithms, each miRNA may have several hundreds to potentially thousands of target mRNAs (25, 32). miRNA profiling has shown the deregulation of miRNAs in different types of human malignancy, some of which are associated with late-stage and high-grade tumors as well as poor prognosis (29, 34, 35), implying that miRNA may play a pivotal role in tumorigenesis and in tumor progression to metastasis.
In the present study, we profiled the miRNA signature of EMT induced by the TGF-β/Smad pathway in normal murine mammary gland (NMuMG) epithelial cells. We further demonstrated that miR-155 is a direct transcriptional target of the TGF-β/Smad4 pathway and mediates TGF-β-induced EMT. The ectopic expression of miR-155 disrupted proper tight junction formation and promoted cell migration and invasion. The knockdown of miR-155 reduced the occurrence of TGF-β-induced EMT and cell migration and invasion. Moreover, RhoA is negatively regulated by miR-155. The restoration of RhoA by using an expression vector cloned without the 3′ UTR eliminated the effects of miR-155-induced phenotypes. Thus, we demonstrated for the first time that miR-155 is regulated by the TGF-β/Smad pathway and plays a role in mammary epithelial cell plasticity through the targeting of RhoA.
Cell line, treatment, and tumor specimens.
NMuMG epithelial cells were purchased from the American Type Culture Collection (Manassas, VA). Stable Smad4 knockdown (pRetroSuper-Smad4-shRNA) and pRetroSuper (pRS) vector-transfected (parental) NMuMG cells were kindly provided by Peter ten Dijke (Leiden University Medical Center, The Netherlands) (5). The cells were maintained in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum. Cells were treated with TGF-β at a concentration of 5 ng/ml for the times indicated in the figures and legends. Cell transfection experiments were performed with Lipofectamine 2000 (Invitrogen). Frozen human primary breast tumor tissues and normal breast tissues were procured anonymously from patients who underwent surgery at H. Lee Moffitt Cancer Center, and each tumor sample contained at least 70% tumor cells as confirmed by the microscopic examination of sections. The tissues were snap-frozen within 15 min of accrual to prevent RNA degradation and stored at −70°C.
miRNA microarray, Northern blot, and quantitative reverse transcription-PCR (qRT-PCR) analyses.
Total RNA from cell lines and breast tumor and normal tissues was isolated using Trizol reagent (Invitrogen). miRNA array profiling was performed as described previously (52). Briefly, oligonucleotide arrays were printed with trimer oligonucleotide probes carrying antisense sequences relative to 515 miRNAs specific to humans and mice on GeneScreen Plus (NEN) membranes. The miRNA expression profiling was performed by the hybridization of the array with [γ-32P]ATP-labeled small RNA probes prepared from TGF-β-treated and untreated NMuMG cells. To ensure the accuracy of the hybridizations, each experimental grouping was hybridized onto three separate membranes. In addition, eight oligonucleotides not matching any known miRNA were used as hybridization controls. Hybridization signals for each spot of the array and background values at 15 empty spots were measured. Raw data were further automatically processed in Microsoft Excel. Hybridization signals that failed to exceed the average background value by more than three standard deviations were excluded from analysis. The data were normalized, and an unsupervised hierarchical clustering analysis with average linkage algorithms was performed with GeneCluster. The results were visualized with TreeView. Differentially expressed miRNAs were identified by using the t test procedure within the significance analysis of the microarray.
For Northern blot analysis, 20 μg of RNA was separated on a 15% denaturing polyacrylamide gel and then electroblotted onto a Zeta-Probe GT blotting membrane (Bio-Rad). Following transfer, the membrane was dried and UV cross-linked. The probes were prepared using the Starfire oligonucleotide labeling system according to the protocol of the manufacturer (Integrated DNA Technologies). The blots were hybridized overnight at 42°C in a buffer containing 5× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate), 20 mM Na2HPO4 (pH 7.2), 7% sodium dodecyl sulfate, 1× Denhardt's solution, and 0.2 mg of salmon sperm DNA/ml and then washed with 1× SSC-1% sodium dodecyl sulfate buffer at 42°C (25). qRT-PCR was performed with the mirVana qRT-PCR detection kit according to the instructions of the manufacturer (Ambion).
Immunofluorescence and immunoblotting.
For immunofluorescence, cells grown to 60 to 80% confluence were washed with phosphate-buffered saline and fixed with 4% paraformaldehyde. Cells were permeabilized with 0.5% Triton X-100 in phosphate-buffered saline prior to the addition of primary and secondary antibodies. The visualization of E-cadherin was performed by staining with mouse anti-E-cadherin (BD Transduction Labs) and then with tetramethyl rhodamine isothiocyanate-conjugated goat secondary anti-mouse immunoglobulin G (IgG; Sigma). The visualization of ZO-1 was performed by staining with rabbit anti-ZO-1 (34) and then with fluorescein isothiocyanate-conjugated goat secondary anti-rabbit IgG (Sigma). Fluorescence imaging was performed by confocal microscopy (Leica), and phase-contrast imaging was performed with an inverted microscope (Nikon). Immunoblotting analysis was carried out as described previously (52).
Isolation and analysis of the miR-155 promoter.
miR-155 is found within the BIC gene on chromosome 21 in humans and chromosome 16 in mice. The genomic structure of human BIC consists of three exons, of which exon 3 encodes miR-155 (8, 43). Based on the transcription start site identified previously by other groups (33, 53), a 1.0-kb putative promoter was amplified by nested PCR using NMuMG cell genomic DNA as the template. The PCR products were cloned into the pGL3-basic vector (Promega) by utilizing the HindIII-MluI sites, and the sequence of the resulting construct was confirmed by DNA sequencing. All-in-one Seq-Analyzer software was used to identify putative Smad4-binding sites within the miR-155/BIC promoter.
ChIP assay.
NMuMG cells were cultured to 70 to 80% confluence and treated with TGF-β at 5 ng/ml for 24 h. Cells were harvested for chromatin immunoprecipitation (ChIP) analysis as described previously (35). Briefly, solubilized chromatin was prepared from a total of 2 × 107 cells. The chromatin solution was diluted 10-fold with ChIP dilution buffer and precleared with protein A beads and preimmune serum. The precleared chromatin solution was divided and utilized in immunoprecipitation assays with either an anti-Smad4 antibody or an anti-IgG antibody. Following washing, the antibody-protein-DNA complex was eluted from the beads. After cross-linking, protein and RNA were removed and the purified DNA was subjected to PCR with primers specific for two putative Smad4-binding sites within the BIC/pri-miR-155 promoter. The sequences of the PCR primers used were as follows: 5′-CCAAAGGAATCACTGGAGGA-3′ and 5′-CCCACAGGTCACTAGGCAAT-3′. Amplified PCR products were resolved by 1.5% agarose gel electrophoresis and visualized by BioImage.
Knockdown of miR-155.
The knockdown of miR-155 in NMuMG cells was achieved by transfection with antisense 2′-O-methyl oligoribonucleotides (ASO) against miR-155 by using Lipofectamine 2000 (Invitrogen). Transfection complexes were prepared according to the instructions of the reagent manufacturer and added directly to the cells at a final oligonucleotide concentration of 10 nmol/liter. Following 36 h of incubation, cells were treated with or without human TGF-β1 (2 ng/ml; RD Systems) for different times and evaluated for tight junctions and EMT phenotypes. ASO were composed entirely of 2′-O-methyl bases and were chemically synthesized by Integrated DNA Technologies (Coralville, IA) with the following sequences: 2′-O-Me-155, 5′-CCCCTATCACAATTAGCATTAA-3′, and 2′-O-Me-scrambled, 5′AAGGCAAGCUGACCCUGAAGU-3′.
Construction of expression plasmid and establishment of cell lines with stable miR-155 expression.
An miR-155 expression plasmid was created according to the protocol for the BLOCK-iT polymerase II miRNA RNA interference expression vector kit. Briefly, the following oligonucleotides were cloned into the pcDNA6.2-GW/miR vector (Invitrogen) and the resulting construct was designated pcDNA6.2-GW/miR-155: 5′-TGCTGTTAATGCTAATTGTGATAGGGGGTTTTGGCCACTGACTGACCCCCTATCAATTAGCATTA-3′and 5′CCTGTAATGCTAATTGATAGGGGGTCAGTCAGTGGCCAAAACCCCCTATCACAATTAGCATTAAC-3′. To generate cells stably expressing miR-155, NMuMG cells were transfected with pcDNA6.2-GW/miR-155 or pcDNA6.2-GW/miR-control vector by using Lipofectamine 2000 (Invitrogen). Following selection with blasticidin, stable clonal cell lines were established and examined for the expression of miR-155 by Northern analysis.
RhoA gene 3′ UTR luciferase reporter assay.
To create individual RhoA gene 3′ UTR luciferase reporter constructs, 60-bp sequences from putative miR-155 binding sites were synthesized and ligated into the pMIR-REPORT vector (Ambion) at SpeI and HindIII sites. To create a full-length RhoA gene 3′ UTR reporter, the following primers were used to amplify the 3′ UTR of the RhoA gene from a mouse cDNA library: 5′ACTAGTGCAGCCTCATGCGGTTAAT-3′ and 5′-AAGCTTTTTTTTTAGAAAACTGCCTTTATTCT-3′. The primers were digested and cloned into the pMIR-REPORT vector (Ambion) at SpeI and HindIII sites. To create a mutant 3′ UTR, point mutations were introduced at the first two miR-155-matching nucleotides within selected putative seeding sequence regions with the following rules: A was changed to T and vice versa, and G was changed to C and vice versa. NMuMG cells in 24-well plates were transfected with 0.10 μg of the pMIR-REPORT-3′UTR/RhoA luciferase reporter, 0.05 μg of the normalization plasmid pCMV-β-galactosidase, and 0.6 μg of miR-155 or non-green fluorescent protein-expressing control vector or 5 ng of TGF-β/ml. Luciferase assays were performed using a luciferase assay system (Promega), and activities were normalized to β-galactosidase activity (52).
Profiles of miRNA expression in TGF-β-induced EMT in NMuMG and Smad4 knockdown NMuMG cells.
The TGF-β/Smad pathway plays a critical role in promoting cancer metastasis. Previous studies have identified a number of protein-encoding genes that are regulated by TGF-β/Smad and mediate TGF-β function (54). Since TGF-β-induced EMT in NMuMG cells is a frequently used cellular model to study the molecular mechanism of cancer metastasis (5, 34) and its miRNA expression signature is not completely understood, we proceeded to profile changes in mRNA expression by using an miRNA microarray in an attempt to identify possible miRNAs involved in TGF-β/Smad-induced EMT and cell migration and invasion. Results from previous studies have shown that different cell lines, including NMuMG cells, with the stable knockdown of Smad4 fail to undergo EMT in response to TGF-β treatment (5). As shown in Fig. Fig.1A,1A, the Smad4 level was decreased by 80% after the stable transfection of NMuMG cells with Smad4 short hairpin RNA. As expected, parental NMuMG cells but not Smad4 knockdown NMuMG cells underwent EMT after TGF-β treatment. Thus, we treated both cell lines with TGF-β for 0, 24, and 36 h to obtain an miRNA signature by using three separate hybridizations. Hybridization to a custom microarray, which contained 515 miRNAs, revealed 28 differentially regulated miRNAs in the parental cells but not in the Smad4 knockdown NMuMG cells between 0 and 24 or 36 h of TGF-β treatment, with a P value of ≤0.05 (Fig. 1B and C). Of the 28 listed miRNAs, 9 were upregulated and 19 were downregulated during TGF-β treatment as demonstrated at both the 24- and 36-h time points (Fig. (Fig.1C).1C). Our array revealed that the members of the let-7 and miR-30 families of miRNAs were consistently downregulated and that clustered miRNAs were often regulated simultaneously (Fig. (Fig.1D).1D). In agreement with previous findings, our array data also showed significant downregulation of miR-200c and miR-205 during the expression of a mesenchymal phenotype (12). miR-155, miR-214, miR-21, and miR-323 were all found to be significantly upregulated. We selected five significantly deregulated miRNAs for validation by Northern blot and qRT-PCR analyses to determine the accuracy of the array data (Fig. (Fig.2A2A and data not shown).
FIG. 1.
FIG. 1.
Profile of miRNA expression in TGF-β/Smad-induced EMT in NMuMG cells. (A) TGF-β induces the cellular morphological change of EMT in control (pRS vector-transfected) but not Smad4 knockdown NMuMG cells. (Upper panels) A Western blot analysis (more ...)
FIG. 2.
FIG. 2.
TGF-β/Smad transcriptionally regulates miR-155. (A) Verification of TGF-β-regulated miRNAs. Parental NMuMG cells were treated with TGF-β for the indicated times and subjected to Northern blot analysis with the indicated probes. (more ...)
We next examined whether the promoters of TGF-β-regulated miRNAs may contain a Smad4-binding element. An analysis of the sequence of a 6-kb DNA region (5 kb upstream to 1 kb downstream from the position corresponding to the end of the pri-miRNA), designated the putative miRNA promoter, was conducted using MATCH and TRANSFAC (22, 29). As indicated in Fig. Fig.1C,1C, both mouse and human promoters of TGF-β-deregulated miRNAs contained one or more Smad4-binding sites, further suggesting that these miRNAs may be regulated by the TGF-β/Smad pathway and play important roles in TGF-β-induced EMT.
miR-155 is a direct target of the TGF-β/Smad pathway.
Since miR-155 is a highly upregulated miRNA in TGF-β-treated NMuMG cells (Fig. (Fig.1C1C and and2A)2A) and its function in metastasis is currently unknown, we investigated if miR-155 is directly regulated by the TGF-β/Smad4 pathway. We cloned a mouse promoter 1.0 kb upstream from the transcriptional start site of the BIC (pri-miR-155) gene into the pGL3-basic vector (33, 53). Sequence analysis revealed two Smad response elements (CAGAC and CTGTCTGT) (23) located at bp −542 and −454 from the transcription start site, which are conserved in the human miR-155 promoter. A luciferase reporter assay revealed that miR-155 promoter activity is induced by TGF-β in parental but not Smad4 knockdown NMuMG cells (Fig. (Fig.2B).2B). Deletion mapping showed that the first Smad4-binding site (i.e., bp −454) is responsible for TGF-β-induced miR-155 promoter activity (Fig. (Fig.2C).2C). To determine whether Smad4 could directly bind to the Smad-binding site within the promoter in vivo, we carried out a ChIP assay. NMuMG cells were treated with or without TGF-β for 24 h and immunoprecipitated with anti-Smad4 antibody. Figure Figure2D2D shows that Smad4 specifically bound to the promoter following TGF-β treatment. These findings indicate that miR-155 is a transcriptional target of the TGF-β/Smad pathway.
miR-155 facilitates TGF-β-induced EMT and tight junction dissolution, as well as cell migration and invasion.
We next assessed whether miR-155 plays a role during TGF-β-induced EMT. First, NMuMG cells were transfected with ASO against miR-155 and control ASO and then treated with or without TGF-β. Figure Figure3A3A shows that miR-155 ASO effectively knocked down miR-155 induced by TGF-β. Accordingly, TGF-β-driven morphological changes were reduced by the knockdown of miR-155 for up to 24 h (Fig. (Fig.3B).3B). Immunofluorescent staining for E-cadherin and ZO-1 at the 24-h time point revealed that tight junction assembly was disrupted by TGF-β in control ASO-transfected NMuMG cells but still intact in miR-155 knockdown cells (Fig. (Fig.3C).3C). In addition, the TGF-β downregulation of E-cadherin was abolished by the knockdown of miR-155 (Fig. (Fig.3D).3D). To further demonstrate the effect of miR-155 on TGF-β function, we established a cell line stably transfected with miR-155 by the transfection of NMuMG cells with pcDNA6.2-GW/miR-155 followed by selection with blasticidin (Fig. (Fig.3A).3A). The ectopic expression of miR-155 alone was not sufficient to induce EMT but did cause disruption in cell polarity and tight junction formations (Fig. 3E and F). Moreover, cells overexpressing miR-155 underwent a complete TGF-β-induced EMT by 12 h compared to the 24 to 36 h it normally takes for the parental control cells in response to TGF-β (Fig. 3E and F). In addition, the E-cadherin level in miR-155-transfected cells was much lower than that in vector-treated cells by 12 h of TGF-β treatment (Fig. (Fig.3G3G).
FIG. 3.
FIG. 3.
miR-155 mediates the effect of TGF-β on EMT. (A) Ectopic expression and knockdown of miR-155. NMuMG cells were transfected with the indicated plasmids and oligonucleotides. Following treatment with (+) or without (−) TGF-β, (more ...)
Furthermore, we assessed the role of miR-155 in cell mobility and invasion. Cell migration was examined using Boyden chambers and “wound healing” assays. Cell invasion was measured using Matrigel-coated Boyden chambers as previously described (28). Triplicate experiments showed that the stable expression of miR-155 nearly doubled cell migration, as illustrated by the results of Boyden chamber and wound healing assays (Fig. 4A, B, and D). Further, cell invasion was also significantly enhanced by the stable overexpression of miR-155 (Fig. 4A and D). In contrast, the knockdown of miR-155 considerably reduced cell migration and invasion (Fig. 4C and D). Taken collectively, these results indicate that miR-155 plays an important role in TGF-β-induced EMT, as well as in cell migration and invasion.
FIG. 4.
FIG. 4.
miR-155 plays a significant role in cell migration and invasion. (A and B) The ectopic expression of miR-155 induces cell migration and invasion. NMuMG cells stably transfected with pcDNA6.2-GW/miR-control (Pre miR-ctrl) and pcDNA6.2-GW/miR-155 (Pre miR-155) (more ...)
RhoA is negatively regulated by miR-155.
Since miR-155 was significantly upregulated in NMuMG cells after treatment with TGF-β (Fig. (Fig.1C1C and and2A)2A) and mediates TGF-β function in EMT (Fig. (Fig.3)3) and cell mobility and invasion (Fig. (Fig.4),4), we proceeded to identify potential targets known to play a role in EMT by using RNA22 miRNA target detection and miRBase databases. Among the candidates surveyed, we found that the 3′ UTR of the RhoA gene, which plays an important role in cell junction formation and stabilization (34, 37, 46, 49), contains three highly conserved regions that may serve as a binding site for miR-155 as determined by the RNA22 algorithm (Fig. (Fig.5A5A).
FIG. 5.
FIG. 5.
RhoA is a target of miR-155. (A) Sequence alignment of miR-155 with the 3′ UTR of the RhoA gene. The seed sequence of miR-155 matches three regions of the RhoA gene 3′ UTR, which are highly conserved among humans (Homo sapiens), mice ( (more ...)
To examine whether the RhoA gene is indeed a target of miR-155, NMuMG cells were transfected with pcDNA6.2-GW/miR-155 and pcDNA6.2-GW/miR-control and selected with blasticidin. Immunoblotting and RT-PCR analyses revealed that RhoA protein but not RhoA mRNA was considerably decreased in miR-155-transfected cells (Fig. (Fig.5B).5B). As expected, TGF-β treatment reduced the expression level of RhoA in NMuMG cells (Fig. (Fig.5C).5C). However, the knockdown of miR-155 largely abolished the inhibitory effects of TGF-β on the RhoA protein level (Fig. (Fig.5C).5C). Since miR-155 downregulates RhoA to drive EMT progression, it is reasoned that the ectopic expression of RhoA can reverse this phenomenon. Indeed, progression toward EMT was hindered when pcDNA3-RhoA, in which the RhoA gene lacked a 3′ UTR, was introduced into stably miR-155-transfected NMuMG cells that were subsequently treated with TGF-β for 24 h. As shown in Fig. Fig.5D,5D, the ectopic expression of RhoA decreased tight junction dissolution induced by miR-155. These results further indicate that RhoA is a target of miR-155 and mediates the contribution of miR-155 to the control of epithelial cell plasticity.
Previous studies have shown that TGF-β induces RhoA protein degradation through the ubiquitin-proteasome pathway (34, 49). Thus, we further examined the significance of protein degradation and miR-155 in the regulation of RhoA by TGF-β. NMuMG cells were transfected with control or miR-155 ASO and then treated with or without TGF-β and proteasome inhibitor MG132. Figure Figure5E5E shows that neither MG132 nor the knockdown of miR-155 alone fully inhibited the TGF-β downregulation of RhoA. However, the combined treatment with MG132 and miR-155 ASO abolished the TGF-β repression of RhoA expression. These results indicate that both the ubiquitin-proteasome pathway and miR-155 mediate the TGF-β regulation of RhoA.
To further demonstrate that RhoA is negatively regulated by miR-155, we generated luciferase reporters that contained each of the three highly conserved seeding sites, along with corresponding mutant forms, and a construct that contained the full length of the RhoA gene 3′ UTR. To determine if any of the three seeding sites respond to miR-155, the reporter plasmids were introduced into NMuMG cells together with the pcDNA6.2-GW/miR-155 or pcDNA6.2-GW/miR-control vector. Following 36 h of incubation, cells were subjected to a luciferase assay. Results from triplicate experiments showed that reporter activity for each site was reduced by the ectopic expression of miR-155, with site 1 exhibiting a more significant response than the two other sites. When these sites were mutated, the luciferase reporter was no longer inhibited by miR-155 (Fig. 5F and G). We then examined if the full-length RhoA gene 3′ UTR is regulated by TGF-β through miR-155. Results from triplicate experiments showed that the full-length reporter responded to TGF-β and that this response was largely abolished when miR-155 ASO was introduced simultaneously into the cells (Fig. (Fig.5H).5H). When the same experiments were carried out with the Smad4 knockdown NMuMG cell line, the full-length reporter did not respond to TGF-β (Fig. (Fig.5I).5I). Furthermore, the antisense full-length 3′ UTR of the RhoA gene failed to respond to miR-155 and TGF-β (Fig. (Fig.5I5I).
miR-155 expression in invasive breast cancer.
Having observed that miR-155 mediates TGF-β-induced EMT and cell migration and invasion, we asked if the expression of miR-155 is associated with cancer invasiveness in human primary breast carcinoma. A total of 62 breast cancer specimens (17 noninvasive and 45 invasive breast carcinomas) and 5 normal breast tissue samples were examined for the expression of miR-155. qRT-PCR (Fig. (Fig.6A)6A) and miRNA locked nucleic acid in situ hybridization (LNA-ISH) (Fig. (Fig.6B)6B) analyses revealed high levels of miR-155 in 41 of 45 invasive tumors but in only 2 of 17 noninvasive cancer tissues (Fig. (Fig.6C).6C). The level of expression of miR-155 in normal breast tissue was very low (Fig. 6A and B). These data further support the findings demonstrating the involvement of miR-155 in EMT and invasion as observed in NMuMG cells and suggest that miR-155 may play a pivotal role in breast cancer metastasis.
FIG. 6.
FIG. 6.
Elevated levels of miR-155 are associated with invasive breast cancer. (A) qRT-PCR analysis of miR-155 expression in normal human breast tissue (lanes N), noninvasive breast cancer tissue, and invasive breast carcinoma. U6 was used as a control. The numbers (more ...)
Many studies have demonstrated previously that the TGF-β pathway plays a critical role in breast cancer metastasis (18, 31, 39, 50). Several TGF-β/Smad-regulated genes have been shown to mediate TGF-β signaling in the control of cellular processes associated with breast cancer metastasis (18, 31, 39, 50). In this study, we report an miRNA expression signature of TGF-β-induced EMT in NMuMG epithelial cells. Twenty-eight miRNAs were found to be significantly deregulated by TGF-β in parental but not Smad4 knockdown NMuMG cells. Further, we showed that miR-155, the most significantly upregulated miRNA, plays an important role in TGF-β-induced EMT and cell migration and invasion. In addition, RhoA was negatively regulated by miR-155 and the restoration of RhoA in miR-155-overexpressing cells decreased TGF-β/miR-155-induced tight junction dissolution. Moreover, high expression levels of miR-155 correlate with invasive breast carcinomas. These findings are important for several reasons. First, they provide an miRNA expression signature of the TGF-β/Smad pathway in mammary gland epithelial cells. Second, the findings of this study establish a direct link between TGF-β/Smad4 and miR-155 during EMT. Finally, this is the first study to describe RhoA as a direct target of miR-155.
miR-155, a product of the BIC gene, is overexpressed in a number of human malignancies, which include B-cell lymphoma and carcinomas of the breast, colon, lung, and ovary (8, 17, 48, 51, 52). Eμ-mmu-miR-155 transgenic mice develop B-cell malignancies (4), whereas miR-155 knockout mice exhibit impaired immune function (40, 45). The transcription factor Pu.1 has been validated previously as a direct target of the miR-155-mediated immunoresponse (47). Moreover, miR-155 represses tumor protein 53-induced nuclear protein 1 (TP53NP1), leading to pancreatic tumor development (10). In addition, the NF-κB and AP-1 transcription factors have been shown previously to regulate miR-155 expression (20, 33, 53). However, the functions of miR-155 in cell migration and invasion have not been investigated. Thus, our study provides the first evidence that miR-155 is upregulated by the TGF-β/Smad4 pathway and mediates TGF-β-induced EMT and cell invasion.
Previous computational and experimental studies have focused on the quality of sequence matching between miRNA and the target (6, 7, 14, 26, 42). miRNAs negatively regulate their target mRNAs through base-pairing interactions, which lead to either mRNA degradation or translational inhibition, depending on the degree of matching between the seed sequence (positions 2 to 7 on the 5′ side) of miRNA and the 3′ UTR of mRNA; e.g., miRNA induces mRNA degradation when the seed sequence perfectly matches the target 3′ UTR or inhibits translation when the sequences are partially identical (6, 7, 14, 26, 42). In addition, recent reports indicate that mRNA secondary structures may contribute to target recognition due to the fact that there is an energy cost associated with the un-base pairing of the messenger required to make the target site accessible for miRNA binding (27, 30). Kertesz et al. showed that site accessibility is as important as base pairing within the seeding region. Effective miRNA function requires nucleic acids flanking the target site, as well as the target itself, to be unpaired in a thermodynamically stable fashion (19). Through the RNA22 algorithm, we found that the seed sequence of miR-155 has the potential to bind in multiple regions within the 3′ UTR of the RhoA gene. Of these sites, we selected three sites that fit the above-mentioned criteria and are highly conserved among species. Further, RhoA gene 3′ UTR reporter assays showed that miR-155 at all three sites significantly diminished luciferase activity. To mimic endogenous conditions more closely, we cloned the full-length RhoA gene 3′ UTR and performed experiments using TGF-β instead of the ectopic expression of miR-155. As expected, the full-length 3′ UTR responded to TGF-β, which was inhibited by the knockdown of miR-155 in the parental NMuMG line but not in the Smad4 knockdown line.
RhoA is the prototypical member of the Rho GTPase family, which regulates many cellular processes, including cellular adhesion, motility, and polarity, and is an important modulator of cell junction formation and stability (34, 37, 46, 49). Previous studies showed that TGF-β induces the disruption of tight junctions, cell polarity, and EMT through the ubiquitination and degradation of RhoA by Smurf1 E3 ligase that is activated by Par6 (34). Our study demonstrated that TGF-β downregulated RhoA protein expression through the upregulation of miR-155 and thus provided an additional molecular mechanism of TGF-β regulation of RhoA (Fig. (Fig.6D).6D). Regulation by miRNAs provides a means for cells to prevent protein translation, a mechanism to quickly prevent the accumulation of proteins by translational inhibition; our findings go hand in hand with earlier findings that TGF-β ubiquitinates RhoA for degradation. In this scenario, the induction of miR-155 halts the translation of RhoA while ubiquitination degrades translated RhoA proteins. Based on computational program predictions, each miRNA may negatively regulate hundreds of protein-encoding mRNAs (6, 7, 14, 26, 42). Two recent studies using gene expression microarray analyses showed that miR-155 and its viral orthologue Kaposi's sarcoma-associated herpesvirus miR-K12-11 negatively regulate more than 180 mRNAs, some of which encode proteins involved in cell migration and invasion, including GSK3, PCSK5, and Rho GTPase-activating protein 21 (11, 41). Thus, RhoA is a major but not the only target that mediates miR-155 function in the control of cell polarity, EMT, and cell invasion contributing to cancer metastasis.
In addition, a previous study described a profile of miRNA expression in human keratinocytes treated with TGF-β. Four miRNAs were upregulated and another four were downregulated by TGF-β (55). Of the eight deregulated miRNAs, only one miRNA (e.g., miR-21) showed a change consistent with our results. This discrepancy may be due to the use of different cell types and the duration of TGF-β treatment. Recent reports have indicated the importance of downregulation for miR-200 family miRNAs during TGF-β-induced EMT (2, 12, 22, 36). In congruence to previous findings, our array also showed the downregulation of miR-200c and miR-205 during a mesenchymal transition (Fig. (Fig.1C).1C). However, the remaining members of the miR-200 family were not detected in our array. This result may be due to the use of different cell lines for the miRNA array analyses in our study and those described in previous reports (2, 12, 36). In addition, we showed frequent upregulation of miR-155 in primary invasive breast cancer tissues. Consistent with this finding, a previous study reported that miR-155 is elevated in a metastatic breast cancer cell line, MDA-MB-231, but not in a nonmetastatic line, MCF-7 (28). We also observed that the knockdown of miR-155 inhibited the TGF-β downregulation of E-cadherin but that the ectopic expression of miR-155 enhanced the TGF-β effects on E-cadherin expression (Fig. 3D and G). Sequence analysis showed no match between the seed sequence of miR-155 and the 3′ UTRs of the E-cadherin, ZEB1, and SIP1 genes (12). Further investigation is required to determine the mechanism of miR-155 downregulation of E-cadherin, although it is likely that the effects are indirect but serve as a useful EMT indicator.
In summary, we demonstrated the miRNA expression signature of TGF-β/Smad-induced EMT in mammary epithelial cells. All 28 deregulated miRNAs contained at least one Smad4-binding site within their putative promoters. miR-155 mediated TGF-β/Smad pathway-induced EMT and cell migration and invasion through the targeting of RhoA. Further, the expression of miR-155 was associated with the invasive phenotype of breast cancer. Thus, miR-155 may be a potential metastatic/prognostic marker and therapeutic target for breast cancer metastasis intervention.
Acknowledgments
We thank Peter ten Dijke for the Smad4 knockdown NMuMG cell lines. We also thank the tissue procurement and DNA sequencing facilities at H. Lee Moffitt Cancer Center for providing cancer specimens and sequencing.
This work was supported by grants from the National Institutes of Health (CA77935 and CA107078) and the Department of Defense (DAMD17-02-1-0671) and Bankhead-Coley grant 07BB-01.
Footnotes
[down-pointing small open triangle]Published ahead of print on 15 September 2008.
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