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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC Jan 16, 2009.
Published in final edited form as:
PMCID: PMC2567132
NIHMSID: NIHMS66600
Pyramidal tract stimulation restores normal corticospinal tract connections and visuomotor skill after early postnatal motor cortex activity blockade
I Salimi,1,2 KM Friel,1 and JH Martin1,3
1Department of Neuroscience, Mt. Sinai Medical School, New York, New York 10032
2Department of Neurosurgery, Mt. Sinai Medical School, New York, New York 10032
3Departments of Neurological Surgery and Psychiatry, Columbia University, New York, New York 10032
N.Y.S. Psychiatric Institute, New York, New York 10032
Correspondence should be addressed to: John H. Martin, Ph.D., Department of Neuroscience, Columbia University, 1051 Riverside Drive, New York, NY 10032, Tel: 212.543.5399, FAX: 212.543.5410, E-Mail: jm17/at/columbia.edu
Motor development depends on forming specific connections between the corticospinal tract (CST) and the spinal cord. Blocking CST activity in kittens during the critical period for establishing connections with spinal motor circuits results in permanent impairments in connectivity and function. The changes in connections are consistent with the hypothesis that the inactive tract is less competitive in developing spinal connections than the active tract. In this study we tested the competition hypothesis by determining if activating CST axons, after prior silencing during the critical period, abrogated development of aberrant corticospinal connections and motor impairments. In kittens, we inactivated motor cortex by muscimol infusion between postnatal weeks 5-7. We next electrically stimulated CST axons in the medullary pyramid 2.5 hours daily, between weeks 7-10. In controls (n=3), CST terminations were densest within the contralateral deeper, premotor, spinal layers. After prior inactivation (n=3), CST terminations were densest within the dorsal, somatic sensory, layers. There were more ipsilateral terminations from the active tract. During visually guided locomotion, there was a movement endpoint impairment. Stimulation after inactivation (n=6) resulted in significantly fewer terminations in the sensory layers and more in the premotor layers, and fewer ipsilateral connections from active cortex. Chronic stimulation reduced the current threshold for evoking contralateral movements by pyramidal stimulation, suggesting strengthening of connections. Importantly, stimulation significantly improved stepping accuracy. These findings show the importance of activity-dependent processes in specifying CST connections. They also provide a strategy for harnessing activity to rescue CST axons at risk of developing aberrant connections after CNS injury.
Keywords: Corticospinal tract, Development, Locomotion, Motor Cortex, Movement [Motion; Motor Activity], Spinal cord
Development of normal motor control depends on forming specific connections between the axons of the corticospinal tract (CST), the key pathway for skilled limb control, and contralateral spinal cord motor circuitry. Achieving this connectivity requires the interplay between guidance cues for target neuron selection (Dottori et al., 1998; Beg et al., 2007) and neural activity for refining connections (Martin, 2005). In kittens, following unilateral inactivation of the motor cortex, the principal origin of the CST in the cat (Ghosh, 1997), the silenced tract is less effective in developing spinal connections than the active tract (Martin, 2005). Silenced CST axon terminations develop a contracted, aberrant dorsal, distribution in the spinal gray matter (Friel and Martin, 2005). CST axons from the active side, in addition to developing contralateral terminations, develop aberrant ipsilateral spinal terminations on the side normally held by the silenced CST. Remarkably, these changes in CST connectivity in the kitten after unilateral motor cortex inactivation replicate the pattern of aberrant CST connectivity in hemiplegic cerebral palsy (Farmer et al., 1991; Carr et al., 1993). Silencing activity also produces permanent impairments in end point control during visually-guided movements (Martin et al., 2000; Friel et al., 2007).
Our recent finding indicates that there is a protracted period of activity-dependent plasticity in the developing CS system (Friel and Martin, 2007). Aberrant connections and motor impairments after unilateral inactivation were restored when neural activity in the untreated, active, motor cortex was blocked later in development. We interpreted these findings in the context of our competition hypothesis (Martin, 2005). Blocking the previously active CST reduced the capacity for its spinal terminations to compete for connections with spinal motor circuits. The previously inactive side, facing less competition, could develop proper spinal connections.
In the present study we conducted a critical direct test of the competition hypothesis by determining if promoting activity-dependent competition by the silenced CS system restores connectivity and function after impaired early CS system development. This experiment also has important clinical significance. Motor impairments in stroke, hemiplegic cerebral palsy, and spinal cord injury are thought to reflect aberrant strengthening or proliferation of undamaged segmental connections that are normally held in check by the CST (Pierrot-Deseilligny and Burke, 2005). We inactivated the motor cortex on one side between postnatal weeks 5-7, the critical period for establishing CST connections. Then, between 7 and 10 weeks, we electrically stimulated CST axons from the inactivated hemisphere in the medullary pyramidal tract (PT), where CST axons converge. This is when the cortical motor representation is established (Chakrabarty and Martin, 2000) and corticospinal connections strengthen (Meng and Martin, 2003; Meng et al., 2004).
Stimulation after inactivation during the critical period substantially improved CST axon connectivity and movement accuracy, providing direct support for the competition hypothesis. Activity-dependent processes can thus be harnessed to rescue corticospinal tract axons at risk of developing aberrant connections. Our findings also provide a strategy for protecting surviving CST axons from forming aberrant connections and function in human infants at risk of developing hemiplegic cerebral palsy.
Animals were obtained from a supplier accredited by the Association for Assessment and Accreditation of Laboratory Animal Care. Kittens were delivered in litters along with a lactating mother at postnatal day 28. Columbia University and the New York State Psychiatric Institute Institutional Animal Care and Use committees approved all experimental procedures. Three groups of animals were compared: controls (CST terminations: n=3; behavior: n=2), unilateral inactivation (CST terminations: n=3; behavior: n=6 for impaired and unimpaired sides), and inactivation and stimulation (CST terminations: n=6; behavior: n=4).
We made every effort to make the general behavioral experiences of the three animal groups as similar as possible. All animals were raised in a highly enriched multilevel cage, with constant access to cat toys and facility and laboratory personnel. Inactivation only and inactivation/stimulation animals were raised during the entire experimental period, which included the period of stimulation and behavioral testing, with at least one other littermate and the mother. Moreover, our published findings on the effects of unilateral inactivation (Friel and Martin, 2005; Friel et al., 2007) examined animal performance on the ladder task daily, between weeks 7-11. Despite this intensive daily enrichment, these animals did not show anatomical or behavioral improvement. Overall, no differences were observed in anatomical or behavioral outcome as a result of subtle differences in day-to-day experiences.
Sensory-motor cortex activity blockade
To block neuronal activity in forelimb primary motor cortex (M1), we infused the GABAA agonist muscimol (10 mM in sterile saline; Sigma Aldridge, St. Louis, MO) using a 28-ga hypodermic needle cannula (Durect Corp., Cupertino, CA)(Martin et al., 1999). The cannula was connected with vinyl tubing (Scientific Commodities; Lake Havasu City, AZ, size 4) to an osmotic minipump (Alzet, model 2002; Durect Corp.) filled with the muscimol solution. Using the metabolic marker cytochrome oxidase, this infusion maximally inhibits a 2.5-3 mm radius of cortex at the infusion site and has a smaller effect for an additional 4-5 mm radius (Martin et al., 1999)(i.e., total spread of inactivation, 6.5-8 mm). Inactivation reduces immunostaining of parvalbumen, a marker of activity that is also activity-dependent (Friel et al., 2007), to a similar extent.
For cannula and pump implantation, animals were administered atropine (0.04 mg/kg i.m.) to reduce tracheal secretions. A mixture of Acepromazine (0.03 mg/kg i.m.) and Ketamine hydrochloride (32 mg/kg i.m.) was given to induce anesthesia. The trachea was intubated and the cats were maintained in an areflexive condition during surgery on 1%-2% isoflurane. Animals were given a broad-spectrum antibiotic (Cefazolin) at the time of surgery and an analgesic (Buprenorphine) afterwards.
A 5 mm wide small craniotomy was made over the forelimb area of M1 (Martin, 1996; Chakrabarty and Martin, 2000). The cannula was inserted 2 mm below the pial surface and was fixed to the skull with screws and dental acrylic cement. The pump, which was implanted in a subcutaneous pocket between the shoulder blades, delivered muscimol at the rate of 0.5 μL/hr during postnatal weeks 5-7. At the end of the infusion period, the cannula was cut from the pump to terminate infusion. We verified that the animals receiving stimulation after inactivation had the same behavioral impairments during and after cessation of the inactivation. All animals in this study showed abolished or substantially impaired contact placing during the infusion period from weeks 5-7, as in our previous studies (Martin et al., 1999; Friel et al., 2007).
Pyramidal tract electrode and electrode implantation
As in our previous study (Salimi and Martin, 2004), we used an array of Teflon insulated stainless steel microwires (50 μm diameter; A-M Systems, Inc.; Carlsborg, WA) for stimulation. The microwires (four wires staggered in length by 250 μm) were attached to a stiff tungsten wire (500 μm outer diameter, rounded at the end) using Carbowax (Fisher Scientific, Pittsburgh, PA) (Palmer, 1978). The Carbowax attachment method permitted detachment of the wires when they were within the brain because it is soluble in a warm aqueous environment; microwires were detached from the carrier above the pia by rinsing the assembly with warm sterile saline.
The microwire electrode array was implanted into the left pyramidal tract (PT) two days after terminating the infusion, and in one animal, one month. Positioning of the electrode array was guided by our previous studies in kittens of the same age (Meng et al., 2004; Salimi and Martin, 2004). The array was inserted stereotactically into the caudal medulla, between 16° and 20° posterior to anterior angulation. The array was positioned 0.5 mm lateral and -4 to -6.00 mm posterior to interaural zero. The array was driven to the skull floor and then raised 0.5 mm to allow for the distance between the skull and the pyramid. A silver wire attached to a cranial bone screw was used as the indifferent. The microwires, together with the connector, were fixed in place using cyanoacrylate and dental acrylic cement. We confirmed the position of the electrodes post mortem with the Prussian blue histochemical reaction to stain iron deposits at the stimulation site (see Supplemental Figure 1A) and by locating marking lesions made just prior to euthanizing the animals on Nissl-stained sections. The day after electrode implantation, we determined the threshold for evoking a motor response from each microwire individually (see below for threshold testing method). We chose one of the microwires as the stimulating electrode based on the lowest threshold for evoking contralateral forelimb movements.
Corticospinal axons tracing
Four weeks before euthanizing each animal, we pressure injected (Picopump, WPI, Sarasota, FL) the anterograde tracers biotinylated dextran amine (BDA; Invitrogen, Inc., Eugene, OR; 10% in PBS) into the forelimb area of M1 of one hemisphere and Lucifer Yellow-dextran amine (LY-DA; Invitrogen, Inc., 1% in PBS) into the other. In each M1, we made three tracer injections, 1.5 mm below the pial surface (300 nL each, separated by 1-1.5 mm). For determining CST projections from inactivated M1, tracer injections were made 3-7 days after cessation of the infusion. The three injections were positioned rostral, lateral, and caudal to the muscimol infusion site, as in our previous studies (Friel and Martin, 2007). This ensured that we were examining the previously silenced projection. BDA was used to label the initially inactivated cortex in all but one animal. Injections in the M1 contralateral to the inactivation were made in locations homotopic to the injections in the inactivated M1. Uptake and/or anterograde transport of BDA are not dependent on neural activity. To control for this concern in our previous study and to examine the effects of activity blockade after the critical period, we injected BDA into M1 at week 7 several days prior to the onset of muscimol infusion (Friel and Martin, 2005). The animal was perfused at week 11, after one month of inactivation and tracer transport. The pattern of CST axon labeling in this animal was identical to the pattern of axonal labeling in age matched controls. Also in that study (Friel and Martin, 2005), we found similar termination patterns irrespective of injecting tracers one week before infusion (trace from 4-7 weeks; inactivate between weeks 5-7) or immediately following (inactivate between weeks 5-7; trace from 7-10 weeks). The pattern of CST axon terminal labeling using LY-DA was always the same as with BDA. Finally, effective intracortical transport occurred during local cortical activity blockade by tetrototoxin (Rhoades et al., 1996).
Stimulation Parameters
A constant current stimulator (model 2100; A-M Systems, Inc) delivered 45 ms trains of stimuli (330 Hz, 20 μsec duration biphasic pulses), 2-3 hours a day, once every 3 seconds, for 21 days at the end of the inactivation period. Average total stimulation time was 2.6 hours/day. Each day we used a current that was just sufficient to evoke a contralateral forelimb movement for each stimulus train. To determine the threshold, we started at zero and increased the current in 5-10 μA intervals until a response was observed. Next we raised the current an additional 20-30 μA and then reduced the current in small steps and noted the value when movements were no longer evoked. The threshold was the average of the currents just evoking a movement during the ascending and descending series. The animals were typically playful while determining the threshold, sometimes making determination difficult. To ensure that we did not underestimate threshold during this period (e.g., the animal's voluntary response was interpreted as a stimulus-evoked response), and risk stimulating below threshold, we sometimes raised the current 5-10 μA. Once the current value was established, the animal was placed in an enclosure for the stimulation period and checked every 10-15 minutes. Animals usually slept for the duration of the stimulation period.
Tissue Preparation and Staining
At the end of the survival period, animals were deeply anesthetized with sodium pentobarbital (30 mg/kg i.v.), injected with heparin (500 units i.v.) and perfused transcardially with warm saline (39°C). This was followed by 4% paraformaldehyde (in 0.1 M PB). The brain and spinal cord were removed, postfixed if necessary and transferred to 20% sucrose in 0.1 M PB overnight. Transverse frozen sections (40 μm) through the C7–T1 spinal cord were cut. Alternate sections were processed for BDA histochemistry and LY-DA immunocytochemistry. Alternate transverse sections were cut through the medulla for Nissl and Prussian blue staining to identify the location of the electrode array tip and marking lesion. Prussian blue permits visualization of iron deposits at the tip of the electrode, which is the result of the passage of current (see Supplemental Figure 1A). Sagittal sections were cut through the cortex in both hemispheres to identify the tracer injection sites.
For BDA visualization, sections were incubated in PBS containing 1% avidin-biotin complex (ABC kit; Vector Labs, Burlingame, CA) according to the manufacturers specifications. Sections were incubated with the chromogen diaminobenzidine (DAB; Sigma, St. Louis, MO) for 6-30 min. For visualization of LY-DA, sections were incubated at 4° overnight in PBS containing 0.01% rabbit anti-LY antibody (Invitrogen Carlsbad, CA) in blocking buffer (3% goat serum in 1× PBS with 0.2% Tween). After rinsing again, sections were incubated for 2 h at room temperature in blocking buffer containing 0.2% anti-rabbit antibody conjugated to peroxidase. After a final rinse, sections were incubated with the chromogen diaminobenzidine (DAB) for 5-30 min.
Anatomical data acquisition and analysis
We traced labeled CS axons (terminal and preterminal axons) and marked the locations of axon varicosities, which are putative presynaptic sites. Axon varicosities were defined, as in our previous study, as a region of the axon that is at least three times axonal width (Li and Martin, 2002). Care was taken to verify that axon varicosities were not kinks or other irregularities in the axon. Using laser scanning confocal microscopy, we have found that CS axon varicosities colocalize the synaptic vesicle protein synaptophysin (Meng et al, 2004) and appose sites containing puncta of the postsynaptic marker PSD-95 (D. Trachtenberg and J. Martin, unpublished observations). Transmission electron microscopy confirmed the confocal observations that the CS axon varicosities are filled with synaptic vesicles and appose sites on the postsynaptic membrane with a postsynaptic density (G. Holstein and J. Martin, unpublished observations). Thus, CS axon varicosities, as defined in this study, are likely to mark presynaptic sites, either en passant or terminal. We traced labeled CS axon terminations and varicosities in gray matter of spinal cord using the program Neurolucida (MBF Bioscience, Williston, VT). LY-DA and BDA labeled axons were traced from transverse spinal sections at 200× magnification. We also traced the gray matter boundary, Rexed's laminae (see below), and anatomical landmarks. Digital tracings of individual sections were corrected for orientation and aligned with one another according to fiduciary marks (intersection between the gray matter above the central canal and the dorsal median septum). The corrected digital images were exported and measured using custom programs written in MATLAB (MathWorks, Natick, MA).
To determine local changes in the amount of labeling, digital files of gray matter labeling on individual sections were divided into 52μm X 52μm square regions of interest (ROI). For each ROI, we computed the mean density of traced axons and marked varicosities. A matrix of mean axon or varicosity density was generated in MATLAB that preserved the mediolateral and dorsoventral dimensions of the distribution of label in the gray matter. Using this matrix, we generated topographic maps of the regional distribution of axonal label and varicosities, where local density is represented according to a color scale, from the lowest density (blue) to the highest (red). To compare the distribution of labeling across animals, we used a commercially-available program (Morpheus) for the Macintosh computer (Apple Computer) to “warp” axon distributions of each individual animal into a standardized spinal gray matter shape. We then averaged individual animal data from each group. Warping (sometimes termed morphing) systematically stretches the border of the starting image (i.e., from a particular animal) to match that of the final (i.e., average). In doing so, the internal spatial structure of the data are also stretched. This is a standard technique for minimizing spatial variability in human brain imaging, and we have used this technique previously for a similar purpose (Campos et al., 2008). For this study, we use warping as a heuristic, rather than analytic, tool to allow the reader to better appreciate differences in axonal distribution between the animal groups in relation to the laminar organization of the spinal cord. Supplemental Figure S2 shows the original gray matter outlines for the warping; the final (i.e., warped) outline is shown in Figures 1C and and3C.3C. To quantify changes in the distribution of label across animals we determined the amount of label within Rexed's laminae. The following laminae were identified, using Rexed's original criteria (Rexed, 1954), on Nissl- or counter-stained spinal cord sections. Laminae 1-3: lamina 3 contains uniformly small cells. Lamina 4: mixture of cell sizes, including very large cells not present dorsally; ventral border corresponded to a horizontal line from the top of the neck of the dorsal horn. Lamina 5: reticulated zone, with reduced cell density. Lamina 6: anatomically corresponds to the base of the dorsal horn (lamina 6 is more commonly included within the intermediate zone; see Results). Lamina 7: includes all ventral territories, exclusive of the medial and lateral motor pools. We distinguished a dorsal region, which corresponded to the portion dorsal to the motor pools, from a ventral region that separated the medial and lateral motor pools. Laminae 8 and 9: medial and lateral motor pools; identified by the presence of motoneurons. Local axon and varicosity density, as a proportion of labeling within each of the laminae, was determined using scripts written in Matlab and Microsoft Excel.
Figure 1
Figure 1
Stimulation after previous M1 inactivation redistributes CS terminations to intermediate and ventral motor laminae. Color coded topographic maps show the local density of axons (A) and varicosities (B) in representative animals. Warped and composite (average; (more ...)
Figure 3
Figure 3
Stimulation following inactivation reduces the density of ipsilateral corticospinal boutons but not axons. Similar format to Figure 1; representative animals are shown in A, B and, a composite average in C (inactivation, n=3; inactivation-stimulation, (more ...)
Behavioral training and testing
All animals were exposed to the laboratory environment several times a week, beginning between weeks 6/7. This enabled obtaining snapshots of performance during the stimulation period and ensured that animals were acclimatized to the environment when testing was done after cessation of stimulation. We examined animals performing a horizontal ladder task (Friel et al., 2007). The ladder was made of Plexiglas (88 cm long, 18.4 cm wide; 9 mm square wide rungs placed every 5.8 cm), with stationary platforms at either end. Cats were placed at one end of the ladder, and meat cubes were placed at the other end. During testing, the cat walked across the ladder from the start platform to the food reward. To prevent cats from memorizing rung position, we placed them at different positions on the platform for each trial while keeping the distance between rungs constant. This resulted in their starting to step on the rungs with either forelimb. Moreover, the first ladder rung to be stepped on differed from trial-to-trial. Animals were first introduced to the task during daily sessions of approximately 10-15 minutes and, once acclimated to the lab and the testing apparatus, most animals were tested twice weekly.
Analysis of behavioral performance
Videotapes of testing sessions were imported into a video editing program (iMovie; for the Apple Macintosh computer) and analyzed for forelimb kinematics to measure endpoint control. We used the same analysis criteria as in our previous studies (Friel et al., 2007). The forelimb contralateral to inactivation/stimulation is termed the affected limb and the other, the unaffected limb. We showed that performance of the unaffected limb was identical to that of animals that did not receive muscimol infusion (i.e., untreated control) or received only infusion of the saline vehicle)(Friel et al., 2007). We measured the distance that the tip of the cat's forepaw extended beyond the edge of the rung of the ladder (termed forward distance). We used the program ImageJ, in combination with the program Afloat (Window management tricks for Mac OS X, Microsoft Visual Studio, Inc) on the Apple Macintosh computer to measure forward distance. Distance measures from the computer screen were converted to cm by scaling according to a calibrated distance on each video file. Trials were not scored if the movement was halted during the step, the movement changed direction, or the animal jumped. Images from the video files were analyzed at 30 Hz. Performance of the inactivation/stimulation animals was compared with data from animals receiving inactivation only, which were reanalyzed from previous studies (Friel and Martin, 2007; Friel et al., 2007).
Statistical Analyses
We used the program Statview for the Apple Macintosh computer to determine the statistical significance of differences. Standard and repeated measures ANOVA were used to compare the dorsoventral distributions of labeling in the inactivation only and inactivation/stimulation groups. Post hoc testing was used to determine if particular levels of the spinal cord, known to be relevant to control by the corticospinal terminations, showed significant differences. Pair-wise t-tests were also used, with correction for multiple comparisons. Linear regression was used to assess significance of changes in threshold currents with stimulation. Unpaired t-tests were used to compare forelimb movement endpoint errors for the inactivated and inactivated/stimulation groups.
PT stimulation redistributes aberrant CS terminations and varicosities ventrally
We examined the regional distribution of labeled CS axon terminals and varicosities, which correspond to putative synapses (Meng et al., 2004), in the C8 spinal segment in each of three animal groups: controls (N=3), unilateral inactivation alone (N=3), unilateral inactivation followed by PT electrical stimulation (N=6). Contralateral CS axon terminals and varicosities are normally densest in the motor territories of the contralateral gray matter—the deepest layer of the dorsal horn (layer 5) and the intermediate zone (layers 6,7)—as shown in a representative control (Figure 1A1, B1) (Friel and Martin, 2005, 2007). This laminar termination pattern is significant because layers 5-7 have been shown to contain premotor interneurons that are active during limb movements in cats (Alstermark and Kümmel, 1990).
Unilateral inactivation between weeks 5-7 results in a redistribution of CS terminals and varicosities from the contralateral motor regions in the deep dorsal horn and intermediate zone to more dorsal, mechanosensory laminae (Figure 1A2, B2), as previously reported (Friel and Martin, 2005). Stimulation of CS axons in the PT from weeks 7 to 10, after earlier M1 inactivation, shifted the distribution of axons and varicosities ventrally to the premotor layers (Figure 1A3, B3). We have previously shown that stimulation of sites adjacent to the PT has no effect on CST axon terminal development (Salimi and Martin, 2004). To assess changes across the population of animals examined, the regional axon varicosity map from each animal was warped into a standardized cervical spinal gray matter outline. Averaged composite maps are shown in part C. Stimulation shifted the focus of maximal axon varicosity density ventrally to the premotor layers (Figure 1C3), from the dorsal (Figure 1C2), mechanosensory, layers. This shows that stimulation of the developmentally impaired CS system partially reestablished connectivity to the premotor laminae of the cord.
We quantified the redistribution of CS projections across all animals by plotting the distributions of axon terminations and varicosities in relation to Rexed laminae (Figure 2). The control distribution (solid line curve) peaked in laminae 5-7dorsal (the deep dorsal horn and upper intermediate zone), whereas the distributions of terminations and varicosities after inactivation alone (Figure 2A, B; light gray bars) peaked in the laminae 1-3 (superficial and middle layers of the dorsal horn), in accordance with the warped image data (Figure 1C1,2). Importantly, after three weeks of daily stimulation following inactivation, the distribution of CS axon terminations and varicosities returned to a pattern similar to controls, with a peak in the more ventral laminae (laminae 4-6; Figure 2A,B; dark gray bars). Importantly, the distributions of CS axon terminations and varicosities for the inactivated only and inactivation/stimulation animals were significantly different (ANOVA; axon terminations: F=3.589; P =0.0052; varicosities: F=4.333; P=0.0015). By contrast, the distribution of axon terminations and varicosities in control and inactivation/stimulation animals were not different (ANOVA; axon terminations: F=1.693; P =0.143; varicosities: F=1.58; P=0.174). Thus, stimulation significantly redistributed the previously silenced CST terminations to be more like that of control animals.
Figure 2
Figure 2
Stimulation following inactivation shifts the distribution of CS axon termination and varicosities ventrally. Mean (±SE) Rexed's laminar distributions of axon terminations (A) and boutons (B) are plotted for inactivation only (light gray; n=3) (more ...)
A characteristic feature of the control distribution is that axon terminations and varicosities were significantly denser in laminae 5 and 6 than in laminae 1-3 (Axons: F=9.353; P<0.0002; Fisher's PLSD laminae 1-3 v/s lamina 5, P=0.0007; lamina 6, P<0.0001; Varicosities: F=6.22; P=0.0019; Fisher's PLSD lamina 5, P=0.0012; lamina 6, P=0.0011). By contrast, unilateral inactivation resulted in significantly fewer terminations and varicosities in laminae 5 and 6 than in laminae 1-3 (Axons: F=4.53; P=0.0078; Fisher's PLSD laminae 1-3 v/s lamina 5, P=0.038; lamina 6, P=0.0065; Varicosities: F=4.389; P=0.009; Fisher's PLSD lamina 5, P=0.016; lamina 6, P=0.0017). Finally, like the controls, animals receiving inactivation/stimulation had significantly more terminations and varicosities in laminae 5 and 6 than in laminae 1-3 (Axons: F=7.624; P<0.0001; Fisher's PLSD laminae 1-3 v/s lamina 5, P=0.0012; lamina 6, P=0.0168; Varicosities: F=6.286; P=0.0005; Fisher's PLSD lamina 5, P=0.0035; lamina 6, P=0.026). Our findings indicate that CS system stimulation between weeks 7-10 redistributes aberrant contralateral corticospinal connections that occurs after inactivation toward a more normal laminar pattern, with significantly fewer terminations dorsally and more terminations ventrally within territories containing premotor interneurons (Alstermark and Kümmel, 1990).
PT stimulation reduces ipsilateral CST varicosities from the active M1
Early in development, cats have ipsilateral CS axon terminations that are largely eliminated by week 7, with the remaining sparse terminations located ventromedially. The CS tract in humans may undergo a similar refinement (Eyre et al., 2001). After unilateral M1 inactivation, the M1 on the other side (which remained active between weeks 5-7; termed the active side), retains its ipsilateral CST terminations (Martin et al., 1999; Friel and Martin, 2007). Figure 3A,B shows representative examples of ipsilateral CS axon terminations and varicosities from the active side (i.e., contralateral to the inactivated M1). In animal receiving inactivation only (A1, B1), the CS projection is present in the ventral dorsal horn and throughout much of the intermediate zone. Maximal density is in the dorsal portion of layer 7. The regional distribution of axon terminations and varicosities is largely segregated from that of the inactive side, which can be seen by comparing the regions of maximal density in Figure 1A2,B2 and Figure 3A1, B1. In the inactivated/stimulated animal, the same laminar axon termination pattern was observed, with the greatest density in the dorsal portion of layer 7. However, varicosities were consistently less dense (Figure 3B2). The warped and averaged regional data for the group of animals examined (Figure 3C) show a substantial reduction in local varicosity density after PT stimulation.
Figure 4 shows the averaged Rexed laminar distributions (laminae 1-9) of ipsilateral CS axon terminations and varicosities. There was a trend towards a reduction in axon terminations in the inactivated/stimulated animals compared with inactivation only (A), but this was not significant (repeated measures ANOVA; F=1.963; P=0.0834). By contrast, the varicosity distribution (B) in the inactivated/stimulated animals was significantly less than the distribution in the inactivated animals only (F=2.696; P=0.0212); the reduction in lamina 7dorsal was significant (t=2.518; p=0.0145). This region is just ventral to where stimulation promotes projections from the inactivated side (see Figure 1). Our findings suggest that stimulation produces reciprocal effects on CS varicosities: as the stimulated varicosities of the contralateral CS projection are augmented in the intermediate zone, the non-stimulated varicosities of the ipsilateral CS projection are diminished. It is interesting to note that after unilateral inactivation ipsilateral bouton density was actually greater than contralateral varicosity density.
Figure 4
Figure 4
Three weeks of daily stimulation following inactivation reduces the density of ipsilateral varicosities from the non-stimulated CST. Same format as Figure 2. Mean (±SE) Rexed's laminar distributions of axon terminations (A) and boutons (B) are (more ...)
Restoration of the regional distribution of CS terminations correlates with progressively reduced motor thresholds during the stimulation period
We hypothesized that if stimulation strengthens synaptic connections of the previously inactivated CS terminals, we should see a reduction in the threshold for evoking movements by PT stimulation at the end of the stimulation period. We determined the threshold for evoking contralateral responses as part of the daily stimulation protocol to establish the current used (see Methods). Linear regression analysis, based on the daily current thresholds, revealed significant correlation coefficients in six of the eight cats. The mean threshold on day one was 146 μA ± 27 μA and on day 21, was 88 μA ± 16 μA. The mean slope was -2.92 μA/day ± 0.79 μA/day. This threshold reduction is substantial larger than the reduction we observed in the threshold for evoking a CST volley by PT stimulation in anesthetized kittens, which was -0.35 μA/day (Meng and Martin, 2003). Figure 5 plots the change in current over the stimulation period for each of the eight cats. These findings suggest that the major contribution to the threshold reduction was daily PT stimulation, with a possible smaller age-dependent change, and that this led to a significant increase in the strength of CS connections with spinal motor circuits.
Figure 5
Figure 5
PT stimulation reduces the electrical threshold of previously silenced CST axons for evoking contralateral motor responses. Lines plot the computed regression lines of changes in current threshold for 8 cats receiving stimulation after prior inactivation (more ...)
Stimulation improves visually-guided locomotion
Unilateral M1 inactivation between weeks 5-7 abolishes the contract placing reaction during the infusion (Martin et al., 2000; Friel et al., 2007). The placing reaction typically returns within several days after cessation of infusion. However, endpoint errors during reaching and visually guided locomotion persist (Martin et al., 2000; Friel et al., 2007). We next determined if PT stimulation ameliorated endpoint control errors in visually guided locomotion. We measured changes in forward distance of the forepaws as animals walked along a horizontal ladder (i.e., distance the front of the paw extends beyond the ladder rung). This is a sensitive and objective measure of the skilled motor impairment produced by M1 inactivation between weeks 5-7. We measured forelimb endpoint at the end of the three week stimulation period (week 10). We compared forelimb endpoints in inactivation/stimulation animals (n=4) with findings in animals with inactivation only (n=6), reanalyzed from videotaped data from our previous studies (Friel and Martin, 2007; Friel et al., 2007).
Figure 6A compares the forward distance of the affected and unaffected forelimbs in inactivation only (light gray) and inactivation/stimulation (dark gray) animals. Mean forward distance for the affected limb at the end of the stimulation period was 2.12 cm ± 0.11 cm (n=4 cats), which was significantly less than after M1 inactivation alone (2.72 cm ± 0.15 cm; n=6 cats) (t=3.185; P=0.015). Mean forward distance for the unaffected limb was 1.58 cm ± 0.06 cm, which is comparable to our prior studies (1.7 cm ± 0.19 cm; (Friel and Martin, 2007; Friel et al., 2007)). Thus, animals expressed significantly smaller mean endpoint errors during visually-guided locomotion if M1 inactivation was followed by three weeks of PT stimulation.
Figure 6
Figure 6
Stimulation following inactivation improves visually guided locomotion by reducing end point errors of the affected limb. Data presented are from 10 week old animals. A. Comparison of forward distance in inactivation only (light gray) and inactivation/stimulation (more ...)
To more directly evaluate improvement of endpoint error produced by stimulation, we computed the difference between the forward distance for the affected and unaffected limbs (i.e., the distance the affected limb overstepped the unaffected limb) for each animal (Figure 6B). M1 inactivation alone resulted in the affected limb overstepping the unaffected limb by 1.54 cm ± 0.12 cm. By contrast, inactivation followed by CS stimulation resulted in a significantly smaller overstep, only 0.54 cm ± 0.058 cm (unpaired T test; t=6.264; P=0.0002). Thus, PT stimulation after prior inactivation substantially improved performance of visually-guided locomotion and this correlated with substantial restoration of CST connectivity with ventral spinal motor circuits.
Activity-dependent competition can be harnessed to rescue CST axons at risk of developing aberrant, and functionally maladaptive, connections with spinal motor circuits and to improve visually-guided movement control. The effects of stimulation of previously silenced CST axons, like alternate inactivation of the initially active motor cortex (Friel and Martin, 2007), achieved a partial restoration of CST connectivity and significantly improved motor function. This similarity can be explained by complementary changes, produced by stimulation and alternate inactivation, in competition between the developing CSTs from each hemisphere for spinal connections. After the initial motor cortex inactivation, CST neurons are at a competitive synaptic ‘disadvantage’ compared with their counterparts from the active hemisphere. Stimulation increased the competitiveness of the disadvantaged CST neurons, whereas silencing of the previously active CS system reduced the synaptic competition faced by the disadvantaged neurons. As discussed below, our finding that activation boosts competition for developing connections with spinal motor circuits has important clinical implications for promoting the function of CST neurons that survive after perinatal injury.
The short daily period of stimulation (2.5 hours) of the impaired CST (i.e, the one inactivated between weeks 5-7) produced effects that were nearly as robust as those produced by the continuous period of inactivation (24 hours daily) of the unimpaired CST (i.e., the one contralateral to the silenced/impaired CST). Reducing competition by inactivation of the previously active M1 likely exerts its effects through spinal circuits originating from the ventromedial ventral horn, where the aberrant ipsilateral CST axons terminate. This may create a permissive environment that “attracts” outgrowth from the previously silenced CST axons into layers of the spinal cord that contain premotor interneurons (Alstermark and Kümmel, 1990). By contrast, stimulation could confer a heightened intrinsic capacity to overcome the growth suppressive effects of the environment.
The conditions under which activity during development provides instructional signaling or constitutive trophic effects are not understood for the CS system, nor are the roles for activity in refining specific circuit features. For the visual system, electrical stimulation of the optic nerve in dark-reared animals resulted in less precise orientation tuning in V1 (Weliky and Katz, 1997). It was proposed that stimulation introduced noise, by synchronous activation of retinal ganglion cell axons in the optic nerve, which disrupted instructional signaling normally provided by spontaneous activity. This raises the question that synchronous activation of the CST, which is likely to be uncorrelated with on-going (“intentional”) activity, could have led to further development of aberrant or maladaptive connections. However, using both anatomical and functional assays, our findings show that this was not the case; raising the level of activity phasically, normalized the topography of CST circuitry in the cord and improved visuomotor control. There are now several examples in which promoting CS system activity leads to specific functional changes, such as chronic motor cortex stimulation augmenting the amplitude of the H-reflex in normal adult rats (Chen et al., 2007) or improving reaching performance after a small motor cortex stroke (Adkins-Muir and Jones, 2003; Adkins et al., 2006). Thus, for the CST it may be sufficient to recruit activity-dependent processes to strengthen CST synapses for achieving adaptive effects. Given our demonstration of the importance of competitive interactions shaping development of connections in the cord, it is tempting to speculate that activity-induced changes in the mature CS system may also be due to promoting synaptic competition by the stimulated CST axons.
CST circuit changes in hemiplegic cerebral palsy
Recent findings of Eyre and colleagues (Eyre et al., 2007) point to a progressive component to hemiplegic cerebral palsy. Normal infants and those that will develop hemiplegic cerebral palsy have similar TMS-evoked motor patterns at 3 months: unilateral TMS evokes bilateral limb motor responses. By 6-12 months, TMS in healthy infants evokes predominantly contralateral effects. By contrast, an aberrant evoked motor pattern emerges in infants that develop hemiplegic cerebral palsy: minimal responses from the damaged cortex and bilateral responses from the undamaged (or less damaged) cortex. We propose that this is explained by an imbalance in activity-dependent competition between the two sides (Martin et al., 1999; Martin and Lee, 1999; Martin, 2005). CST neurons that are initially less competitive in maintaining connections with spinal motor circuits become progressively less competitive as they lose more connections later in development.
CST axons that survive after unilateral perinatal trauma may initially develop sparse and aberrant connections, but have the potential to regain normal connectivity. This potential could be achieved either by enhancing their ability to compete for synaptic space or by reducing synaptic competition that they face from other sources. Direct CST activation, as we have used in this study, or repetitive TMS could be used to activate CST axons to enhance competitiveness. Constraint induced movement therapy (Charles and Gordon, 2005; Gordon et al., 2006) or deactivating the intact cortex using an inhibitory TMS pulse sequence (Di Lazzaro et al., 2005) might reduce competition. It is important to note that stimulation also promotes CST outgrowth and increases connection strength in adult rats (Brus et al., 2007), showing that the effects of activity on CST axons are not restricted to the developing nervous system (Salimi and Martin, 2004). This, combined with the findings of protracted CST development (Armand et al., 1997; Koh and Eyre, 1988; Nezu et al., 1997; Olivier et al., 1997), suggest that stimulation could rebalance connectivity late in development or in maturity.
CS circuitry and visual guidance of limb control
Normalization of contralateral connectivity in the cervical spinal cord after stimulation is likely to be key to improving visuomotor control. This is consistent with recovery of motor function after stroke in mature humans, which stresses the importance of the ipsilesional cortex (Ward and Cohen, 2004). We have focused on the contralateral CST projection originating from M1, however, as in humans after M1 lesion, premotor areas may contribute to improved performance in cats after stimulation. While we have examined the direct segmental CST projection, restitution of other CS system circuits—such as between CST axons and propriospinal systems (Pierrot-Deseilligny and Burke, 2005)— could also contribute to improvement. This is because CST axons have collateral branches that project to multiple levels of the spinal cord (Shinoda et al., 1976; Shinoda et al., 1986; Casale and Light, 1991). It is also theoretically possible that PT stimulation antidromically activated collateral branches of CST axons that project into the brain stem (Keizer and Kuypers, 1984; Ugolini and Kuypers, 1986).
Stimulation also reduced the density of ipsilateral varicosities. Perhaps with a longer period of stimulation, ipsilateral axons would also have been reduced. Normalization of the contralateral terminations (i.e., ventral shift in connections) occurs in association with normalization of ipsilateral terminations (i.e., reduction in connections). It is plausible that the two are causally linked, via reciprocal spinal (Pierrot-Deseilligny and Burke, 2005) or supraspinal circuitry. However, it is unclear whether a reduction in ipsilateral connectivity is beneficial for motor recovery. An adaptive role for the ipsilateral CS system in recovery after unilateral CS system injury in humans is controversial (Ward and Cohen, 2004; Staudt, 2007). In the cat, aberrant ipsilateral CST projections after prior inactivation are adaptive, and their presence improves performance of the affected limb (Martin et al., 2000). In the intact spinal cord, ipsilateral CST axon terminations comprise 10-15% of the population (Brosamle and Schwab, 1997; Lacroix et al., 2004), and thus are likely to be adaptive because of their large number. Ipsilateral control can also be mediated by indirect routes, via brain stem motor paths. Studies of Edgley and colleagues (Edgley et al., 2004; Jankowska and Edgley, 2006; Jankowska et al., 2006) stress the importance of corticoreticulospinal networks in transmitting control signals to spinal motor circuits bilaterally. Moreover, ipsilateral actions could occur via callosal projections. To a certain degree, movements of one limb routinely depend on control of the other limb. Whether explicit, as during bimanual manipulations, or as a consequence of postural interactions, such as stabilizing an object with one limb and taking action with the other, bilateral control is likely reflected in some degree of control by the ipsilateral CS system.
Combinatorial rehabilitation approaches with hemiplegic cerebral palsy
Stimulation of the damaged nervous CST likely targets multiple repair mechanisms. Lesion of the CST on one side in mature rats leads to ipsilateral outgrowth of intact CST axons into the territory occupied by the previously lesioned axons, and stronger connections (Brus et al., 2007). Stimulation of CST axons in the intact nervous system also leads to outgrowth and, importantly, significantly stronger CST connections than after injury. Combining stimulation and injury resulted in significantly stronger connections than either of the two alone. Thus, stimulation significantly augments lesion-induced plasticity, possibly through separate effects on axonal outgrowth and connection strength. With this in mind, development of therapies for prevention and rehabilitation of motor control impairments in patients with cerebral palsy could combine different mechanisms, each at the level of motor circuitry. CST stimulation could be combined with behavioral training (Butefisch et al., 2004) to target circuits that are concurrently activated by the stimulus and recruited by task performance. Stimulation could be combined with constraint inducted movement therapy of the unaffected side to increase the ‘activation dosage.’ Manipulation of activity-dependent processes globally, as with constraint or motor performance, or selectively, as with CST stimulation—alone, or in combination—could prove fruitful in promoting motor function after perinatal CS system injury.
Supplementary Material
Supp1
Supplemental Figure 1. Histochemical staining of iron deposits (dark region circled by white dotted line) using the Prussian blue reaction around the electrode confirms the location of electrode in the medullary pyramid (black lines). Scale bar 500 μm.
Supp2
Supplemental Figure 2. A. Outlines of spinal gray matter used in warping the composite shape in Figure 1C. B. Spinal gray matter outlines used for warping the composite outline in Figure 3C.
Acknowledgments
We would like to acknowledge the assistance of Xiu Li Wu for histo- and immunohistochemistry, Dan Raz for developing the imaging software, Germa Asfaw and Dr. M. Osman for veterinary care, and R. James Salway, Erin Nunnink, Cedrick Mendoza-Tolentino, Brandon Shulman, and Bernice Sist for help with training and analysis. We thank Jason Carmel for helpful comments on the manuscript. Supported by: NIH NS33835 (JM) and the UCP Research and Education Foundation (IS).
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