Search tips
Search criteria 


Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Cell. Author manuscript; available in PMC 2009 May 30.
Published in final edited form as:
PMCID: PMC2553566

The microRNA miR-1 regulates a MEF-2 dependent retrograde signal at neuromuscular junctions


We show that miR-1, a conserved muscle specific microRNA, regulates aspects of both pre- and post-synaptic function at C. elegans neuromuscular junctions. miR-1 regulates the expression level of two nicotinic acetylcholine receptor (nAChR) subunits (UNC-29 and UNC-63), thereby altering muscle sensitivity to acetylcholine (ACh). miR-1 also regulates the muscle transcription factor MEF-2, which results in altered pre-synaptic ACh secretion, suggesting that MEF-2 activity in muscles controls a retrograde signal. The effect of the MEF-2-dependent retrograde signal on secretion is mediated by the synaptic vesicle protein RAB-3. Finally, acute activation of levamisole-sensitive nAChRs stimulates MEF-2-dependent transcriptional responses, and induces the MEF-2-dependent retrograde signal. We propose that miR-1 refines synaptic function by coupling changes in muscle activity to changes in pre-synaptic function.

Keywords: microRNA, miR-1, synapse, nAChR, nicotinic receptors, acetylcholine, MEF2, MEF-2, RAB-3, Rab3, retrograde, synaptic transmission, C. elegans


Faithful synaptic transmission requires that neurotransmitter release is matched to post-synaptic receptor function. This coordination is achieved through bidirectional signaling between pre- and post-synaptic cells. A variety of mechanisms are proposed to mediate coupling of pre- and post-synaptic function including signaling by retrograde messengers and trans-synaptic adhesion molecules (Davis and Bezprozvanny, 2001; Kalinovsky and Scheiffele, 2004; Scheiffele, 2003). By bridging the pre- and post-synapse, these molecules are considered likely candidates for coordinating synapse refinement. Many questions remain to be answered about this process. What upstream signaling pathways are directly involved in coupling changes across the synapse? What downstream molecular targets mediate the pre- and post-synaptic changes? Here we provide evidence that a conserved muscle microRNA, miR-1, is involved in this process.

MicroRNAs regulate several aspects of neuronal development (Kosik, 2006). In C. elegans, left/right asymmetric cell fate of chemosensory neurons during development is controlled by microRNAs (Johnston and Hobert, 2003), as is the switch to neuronal-specific gene expression during vertebrate neurogenesis (Cao et al., 2006). In zebrafish, a single microRNA is required for development of the nervous system (Giraldez et al., 2005). While microRNAs were originally identified based on their regulation of developmental processes, it is becoming apparent that microRNAs also regulate properties of mature tissues. For example, in cultured rodent neurons, microRNAs have been implicated in coupling the response to neurotrophins with changes in the size of dendritic spines (Schratt et al., 2006).

The early lethality of genetic mutants in the microRNA biosynthetic pathway and the limited availability of conditional knockouts of individual microRNAs has prevented detailed analysis of post-developmental roles for microRNAs such as miR-1. The sequence and muscle-specific expression of miR-1 are conserved in all species examined (Nguyen and Frasch, 2006). Fly and mouse miR-1 knockouts are lethal (Sokol and Ambros, 2005; Zhao et al., 2007), underscoring its importance as a developmental regulator. C. elegans mir-1 mutants are viable, allowing the dissection of miR-1 function in adult animals. We show that miR-1 regulates synaptic transmission at neuromuscular junctions, and that it does so by regulating nicotinic acetylcholine receptors (nAChR) and the generation of a retrograde signal that modulates the function of pre-synaptic terminals.


The C. elegans genome contains a single miR-1 ortholog with perfect sequence conservation (Lee and Ambros, 2001). To analyze the expression of mir-1, we generated a transcriptional reporter construct in which the mir-1 promoter drives expression of GFP. This construct expressed GFP in both pharyngeal and body muscles, with no apparent expression in other tissues, including neurons (Fig. 1A). Thus, miR-1 expression is restricted to muscles in flies, mice, and worms. Two independent mir-1 deletion mutants (gk276 and tm1635) were viable as homozygotes and did not display any overt behavioral abnormalities. Muscle cell numbers and morphology were superficially normal in both mir-1 mutants (data not shown). These results suggest that lack of miR-1 did not grossly alter muscle development in worms.

Figure 1
miR-1 affects muscle sensitivity to ACh. A) A transcriptional reporter containing a 3.7kb mir-1 promoter driving expression of GFP showed expression in body-wall and pharyngeal muscles. B) Body-wall muscle expresses two classes of nAChR: the ACR-16/α7 ...

Decreased muscle responsiveness to nicotinic agonists in mir-1 mutants

Worm body muscle contracts in response to activation of nicotinic acetylcholine receptors (nAChRs). Body muscles express two classes of nAChRs (Richmond and Jorgensen, 1999) (Fig. 1B). Levamisole-sensitive nAChRs (LevRs) are hetero-pentamers containing alternative α-subunits (UNC-38, UNC-63, and LEV-8) and non-α-subunits (UNC-29 and LEV-1) (Brown et al., 2006). Body muscles also express ACR-16/α7 homo-pentamers, which are insensitive to levamisole (Francis et al., 2005; Touroutine et al., 2005). To determine if some aspect of muscle function was altered, we assayed the sensitivity of mir-1 mutants to levamisole. Levamisole binds to and activates LevRs, leading to muscle contraction and subsequent paralysis. Homozygous mir-1(gk276) and mir-1(tm1635) mutants were both resistant to the paralytic effects of levamisole (Fig. 1C, data not shown). Consistent with these behavioral results, levamisole-evoked currents recorded from body wall muscles were significantly reduced in both mir-1 mutants (Fig. 1D, E). The function of ACR-16 channels can be assayed by measuring ACh-evoked currents in unc-29 mutants (Francis et al., 2005; Touroutine et al., 2005). The Ach-evoked currents in unc-29 single mutants and unc-29 mir-1 double mutants were indistinguishable (Fig. 1F, G). These results suggest that the number or activity of LevRs on the surface of body muscles was decreased in mir-1 mutants, whereas ACR-16 receptors were unaffected.

Agonist-evoked currents are likely to be mediated by both synaptic and non-synaptic nAChRs. To determine if miR-1 regulates the function of synaptic receptors, we recorded excitatory post-synaptic currents (EPSCs) produced by the endogenous activity of motor neurons. We observed a 23% decrease in the amplitude of endogenous EPSCs in mir-1 mutants (Fig. 1H, I, p=0.002, Student’s t-test), as would be predicted if loss of miR-1 reduces the activity of synaptic nAChRs

Approximately 80% of the excitatory post-synaptic current in body muscles is carried by ACR-16/α7 homo-pentamers, with LevRs mediating the remaining 20% (Francis et al., 2005; Touroutine et al., 2005). Consequently, the decreased endogenous EPSC amplitudes observed in mir-1 mutants could result from a change in either the LevRs, ACR-16 receptors, or both. To distinguish between these possibilities, we recorded EPSCs from double mutants lacking miR-1 and one of the two classes of nAChRs. The amplitude of endogenous EPSCs in acr-16; mir-1 double mutants and in unc-29 mir-1 double mutants were significantly lower than those observed in the corresponding single mutants, acr-16 and unc-29 respectively (Supplementary Figs. 1 and 2) These results suggest that miR-1 regulates the activity of synaptic ACR-16 and LevRs.

Different classes of nAChRs can often be distinguished by their kinetics. Consistent with this idea, we found that the decay kinetics of endogenous EPSCs in unc-29 mutants were significantly faster than those recorded from wild type controls (Supplementary Fig. 2). These results indicate that synaptic currents mediated by ACR-16 receptors have faster decay kinetics than those mediated by LevRs. Similarly, in mir-1 mutants, the decay kinetics of endogenous EPSCs were significantly faster than those recorded from wild-type controls, and were virtually identical to those observed in unc-29 mutants (Fig. 1, Supplementary Fig. 2). These data are consistent with a miR-1-dependent change in the activity of synaptic LevRs. Interestingly, EPSC decay time constants in mir-1 unc-29 double mutants were faster than in unc-29 single mutants (Supplementary Fig. 2), suggesting that the kinetics of ACR-16-mediated synaptic currents are also affected in mir-1. These results suggest that miR-1 regulates sensitivity of body muscles to ACh.

miR-1 regulates nAChR subunit abundance

The decreased function of LevRs in mir-1 mutants could be caused by altered abundance of receptor subunits. Consistent with this idea, we found predicted miR-1 binding sites in the 3’UTRs of the unc-29 and unc-63 mRNAs (Fig. 2A). These putative miR-1 binding sites were conserved in the orthologous genes in the nematode C. briggsae (Supplementary Fig. 3). The bioinformatic algorithm mirBase also identified unc-63 as a predicted miR-1 target (Griffiths-Jones et al., 2006). By contrast, miR-1 binding sites were not found in the 3’UTRs of mRNAs encoding other muscle nicotinic receptor subunits, including UNC-38, LEV-8, LEV-1, and ACR-16.

Figure 2
miR-1 regulates nAChR subunit abundance. A) Sequence alignment of miR-1 binding sites (predicted using the Miranda algorithm) in the unc-29 and unc-63 3’UTRs (Enright et al., 2003). (B–F) Abundance of endogenous UNC-29, UNC-38, and UNC-63 ...

We next investigated whether miR-1 regulates unc-29 and unc-63. First, we analyzed expression of endogenous subunits by immunostaining (Fig. 2B–D) and immunoblotting (Fig. 2E, F). In both assays, we observed increased abundance of endogenous UNC-29 and UNC-63 subunits in mir-1 mutants, whereas the abundance of a control subunit (UNC-38) was unaltered. To determine if regulation of UNC-29 abundance was mediated by the predicted miR-1 binding sites in the 3’UTR, we made a reporter construct in which the unc-29 3’UTR was appended to GFP. When this construct was expressed in body muscles, we observed increased GFP abundance in mir-1 mutants compared to wild type (Fig. 2G, H). By contrast, GFP abundance was unaltered in mir-1 mutants for reporter constructs containing the unc-38 3’UTR, or a mutagenized unc-29 3’UTR in which nucleotides in the three predicted miR-1 binding sites were scrambled (Fig. 2G, H). These results support the idea that the unc-29 and unc-63 mRNAs are miR-1 targets, while the unc-38 mRNA is not.

Increased UNC-29 and UNC-63 expression decreases muscle sensitivity to levamisole

Decreased LevR function in mir-1 mutants could be caused by increased abundance of UNC-29 and UNC-63, or by misregulation of other miR-1 targets. To distinguish between these possibilities, we analyzed the effect of increasing expression of these subunits in wild-type animals. unc-29 and unc-63 cDNAs were fused to the unc-54 3’UTR, which lacks predicted miR-1 binding sites. When the UNC-29 and UNC-63 constructs were co-expressed in body muscles of wild-type animals, we observed resistance to the paralytic effects of levamisole (Fig. 3A), and a significant decrease in the amplitude of levamisole-evoked currents recorded from body muscles (Fig. 3B, C). By contrast, no change in levamisole sensitivity was observed when either construct was expressed alone in wild-type animals, nor when both constructs were co-expressed in mir-1 mutants (Fig. 3A). Thus the levamisole resistance phenotype of mir-1 mutants can be explained by the coordinate up-regulation of UNC-29 and UNC-63. Over-expression of UNC-29 and UNC-63 was not sufficient to cause a change in either the amplitude or kinetics of endogenous EPSCs (Supplementary Fig. 4), suggesting that other miR-1 targets may contribute to these defects. These results are consistent with prior studies showing that changes in subunit composition alter the kinetics, conductance, and agonist affinity of mammalian nAChRs (Millar, 2003).

Figure 3
Over-expression of UNC-29 and UNC-63 decreases sensitivity to levamisole. A) The time course of levamisole (0.2 mM)-induced paralysis was compared for wild-type, mir-1(gk276), and transgenic animals over-expressing UNC-29 [unc-29(xs)], UNC-63 [unc-63(xs) ...

Synaptic transmission is reduced in mir-1 mutants

The decreased muscle responsiveness to ACh prompted us to consider whether miR-1 regulates synaptic transmission at NMJs. Steady-state acetylcholine (ACh) secretion in living worms can be assayed by measuring resistance to the acetylcholine esterase inhibitor aldicarb. Aldicarb treatment causes accumulation of ACh at neuromuscular junctions (NMJs), leading to acute paralysis. Mutations that decrease ACh secretion confer resistance to aldicarb-induced paralysis (Miller et al., 1996). We found that mir-1 mutants were resistant to aldicarb (Fig. 4C), as would be predicted if ACh release had been decreased. To more directly assay ACh release, we recorded EPSCs from body muscles. We found that the EPSC amplitudes and the total synaptic charge transfer evoked by a depolarizing stimulus were significantly decreased in mir-1 mutants (Fig. 4A, B; Supplementary Table 1).

Figure 4
Decreased pre-synaptic ACh secretion in mir-1 mutants. Stimulus evoked responses were recorded from adult body wall muscles in 1mM CaCl2, 4 mM MgCl2. Average stimulus evoked responses (A) and EPSC amplitudes (B) are compared for Wild type (n=18) and ...

In principle, altered EPSCs and aldicarb responses could arise from either a change in pre-synaptic release of ACh, or from the decreased activity of muscle LevRs. We did several experiments to distinguish between these possibilities. First, the reduction in EPSC amplitude in mir-1 mutants (55%) was significantly greater than that observed in unc-29 mutants (20%) (Francis et al., 2005). Second, to control for changes in muscle sensitivity, we measured quantal content. Changes in muscle sensitivity to ACh alters quantal size, i.e. charge transfer that occurs following release of a single synaptic vesicle. The number of vesicles released following a stimulus can be estimated by measuring the quantal content, which measures the ratio of post-synaptic charge transfer that occurs following a depolarizing stimulus to that which occurs during an endogenous EPSC. We found that quantal content was also significantly reduced in mir-1 mutants (Supplementary Table 1). These results suggest that the decreased stimulus-evoked EPSC observed in mir-1 mutants is unlikely to result from a change in muscle sensitivity.

The pool of primed synaptic vesicles can be measured by evoking fusion with hypertonic sucrose (Rosenmund and Stevens, 1996). We found that the EPSC evoked by hypertonic sucrose was not altered in mir-1 mutants compared to wild-type controls (Fig. 4D, E), suggesting that the decreased ACh secretion observed in mir-1 mutants was not caused by a change in vesicle priming.

As an independent assay of pre-synaptic function, we also recorded endogenous EPSCs. The rate of endogenous EPSCs was significantly reduced in mir-1 mutants (~50% wild-type rate, p<0.0001, Student’s t-test) compared to wild-type controls (Fig. 4G–I). The decreased endogenous EPSC rate was not a secondary consequence of the decreased EPSC amplitude (Fig. 1H), because only a 19% change in EPSC rate would be predicted to result from a 23% decrease in amplitude. These results suggest that mir-1 mutants have a pre-synaptic defect leading to decreased ACh secretion.

Synapse density is not altered in mir-1 mutants

The secretion defects observed in mir-1 mutants might be caused by a decrease in the number of cholinergic NMJs. To address this possibility, we examined the localization of two active zone proteins, SYD-2 / α-Liprin (GFP-tagged), UNC-10 / RIM1 (endogenous protein visualized with anti-UNC-10 antibodies), and a synaptic vesicle protein (SNB-1 / Synaptobrevin, GFP-tagged) (Koushika et al., 2001; Zhen and Jin, 1999). We found that all three pre-synaptic proteins had similar distributions in wild-type and mir-1 mutants. In particular, the densities of SYD-2 and SNB-1 puncta in wild-type and mir-1 mutants were indistinguishable (Supplementary Fig. 5). We also examined the distribution of a post-synaptic protein, GFP-tagged ACR-16, and found that puncta density was not reduced in mir-1 mutants (Wild type: 3.5 +/− 0.1 puncta/10µm, mir-1: 3.6 +/− 0.1 puncta/10µm, p= 0.59 Student’s t-test). Consequently, loss of miR-1 did not grossly alter the distribution of pre- and post-synaptic proteins, nor the apparent number of cholinergic NMJs.

Interestingly, the fluorescence intensities of SYD-2 and SNB-1 were significantly altered in mir-1 mutants: SNB-1 fluorescence decreased (20%, p< 0.001, Student’s t-test), whereas SYD-2 fluorescence increased (39%, p<0.0001, Student’s t-test). These changes in fluorescence are unlikely to be caused by changes in transcription, since opposite changes in fluorescence were observed despite the fact that both transgenes utilized the same promoter (unc-129). The altered abundance of SNB-1 and SYD-2 provides further evidence that mir-1 mutants have pre-synaptic defects.

MEF-2 mediates the pre-synaptic function of miR-1

What miR-1 target mediates the pre-synaptic defects observed in mir-1 mutants? The rate of endogenous EPSCs was not significantly altered in transgenic animals driving over-expression of UNC-29 and UNC-63 in body muscles (Supplementary Fig. 4), indicating that the pre-synaptic defect was not caused by changes in the abundance of nAChR subunits in muscles. LevRs are expressed both in muscle and neurons (Brown et al., 2006); however, unc-29 mutants have a normal rate of endogenous EPSCs [Wild type (n=9) 11.4 +/− 2 Hz, 19.7 +/− 2.5 pA; unc-29(x29) (n=7) 13.3 +/− 2.2 Hz, 19.8 +/− 5 pA]. Thus, the pre-synaptic effects of miR-1 are unlikely to be mediated by altered function of pre-synaptic LevRs.

Since changes in UNC-29 and UNC-63 function cannot account for the observed decrease in ACh secretion, we searched for other miR-1 targets that might mediate this change in secretion. Members of the Mef2 family of transcription factors were recently shown to regulate formation of excitatory synapses in cultured rodent neurons (Flavell et al., 2006; Shalizi et al., 2006). This prompted us to investigate whether MEF-2 might function downstream of miR-1 in regulating pre-synaptic function. There is a single mef-2 ortholog in C. elegans which is ubiquitously expressed, and mutants lacking MEF-2 are viable (Dichoso et al., 2000). We identified two miR-1 binding sites in the 3’UTR of the mef-2 mRNA, both of which were conserved in C. briggsae (Supplementary Fig. 6). In addition, mef-2 was identified as a predicted miR-1 target in a genome-wide computational prediction of microRNA targets (Lall et al., 2006). The 3’UTR of the mouse Mef2a mRNA was previously shown to inhibit translation, and we found that it also contains three putative miR-1 binding sites (Supplementary Fig. 7) (Black et al., 1997).

These data suggest that the mef-2 might be regulated by miR-1. Consistent with this idea, expression of a GFP reporter construct containing the mef-2 3’UTR in body muscles was significantly increased in mir-1 mutants (Supplementary Fig. 6) whereas GFP levels were unaltered when this construct was expressed in motor neurons (Wild-type: 78.7 +/− 0.9 A.U, mir-1: 83.0 +/− 7.0 A.U., p= 0.58 Student’s t-test). Mouse Mef2 promotes expression of miR-1 (Rao et al., 2006; Zhao et al., 2005); however, we found no evidence for MEF-2-dependent changes in the expression of a miR-1 transcriptional reporter (data not shown).

To determine if MEF-2 mediates the pre-synaptic functions of miR-1, we analyzed mir-1 mef-2 double mutants (Fig. 5). Single mutants lacking MEF-2 had a 26% decrease in stimulus-evoked EPSC amplitude (p=0.02, Student’s t-test) (Fig. 5A, B) and a normal rate of endogenous EPSCs (Fig. 5C–E). The effects of the mir-1 mutation on stimulus-evoked EPSC amplitude and endogenous EPSC rate were eliminated in mir-1 mef-2 double mutants, and in both cases the mir-1 mutant defects were restored by transgenic expression of MEF-2 in body muscles (Fig. 5A–G). By contrast, the mef-2 mutation did not correct the decreased levamisole-evoked current in mir-1 mutants (Supplementary Fig. 8), nor did it correct the defect in endogenous EPSC decay rate (Fig. 5E, data not shown). However, the defect in endogenous EPSC amplitude was corrected in mir-1 mef-2 double mutants. These results suggest that muscle expression of MEF-2 mediates all of the presynaptic effects of miR-1, but does not mediate many of its post-synaptic effects. These results further suggest that miR-1 and MEF-2 act in body muscles to control a retrograde signal that regulates pre-synaptic ACh secretion.

Figure 5
MEF-2 mediates the pre-synaptic effects of miR-1. (A,B) Stimulus evoked responses were recorded from wild-type (n=18), mef-2(gv1) (n=8), mir-1(gk276) (n=18), mir-1 mef-2 (n=9), and mir-1 mef-2 double mutants carrying a transgene driving mef-2 expression ...

RAB-3 mediates the pre-synaptic function of miR-1 and MEF-2

The pre-synaptic secretion defect observed in mir-1 mutants was associated with a dramatic increase in the synaptic abundance of YFP-tagged RAB-3. The increased YFP::RAB-3 abundance was eliminated in mir-1 mef-2 double mutants, and was restored by transgenes driving mef-2 expression in body muscles (Fig. 6A–F). RAB-3 is a small GTPase that associates with synaptic vesicles in a GTP-dependent manner (Sudhof, 2004). Decreased secretion can result from either increased or decreased RAB-3 expression (Schluter et al., 2004; Thiagarajan et al., 2004). This prompted us to test whether the decreased secretion observed in mir-1 mutants was caused by a decrease in RAB-3 activity. If this were the case, one would expect that the secretion defects observed in mir-1 and rab-3 single mutants would be very similar, and that these defects would not be additive in mir-1; rab-3 double mutants. Consistent with this idea, we found that stimulus-evoked EPSC amplitudes (Fig. 6G, H), and the rates of endogenous EPSCs (Fig. 6I, J) were indistinguishable in mir-1 and rab-3 single mutants, and additive effects were not observed in mir-1; rab-3 double mutants (Fig. 6G–L). These results support the idea that the pre-synaptic defects observed in mir-1 mutants are caused by decreased RAB-3 activity.

Figure 6
RAB-3 is the pre-synaptic effector of the MEF-2-dependent retrograde message. A–E) YFP-tagged RAB-3 (YFP::RAB-3) was expressed in the cholinergic DA motor neurons (using the unc-129 promoter) in the indicated genotypes. Scale bars indicate 10 ...

Levamisole treatment induces MEF-2-dependent changes in transcription

The transcriptional activity of mouse Mef2 is regulated by neuronal activity (Flavell et al., 2006; Shalizi et al., 2006). If MEF-2 transcriptional activity were similarly activity-dependent, the miR-1/LevR/MEF-2 pathway described here could provide a mechanism to couple changes in muscle activity to changes in pre-synaptic properties. To test this idea, we developed assays for MEF-2-dependent transcriptional activity. We identified several genes whose mRNA abundance is MEF-2-dependent, using gene chip analysis (D.S., K.T-P, and J.K., unpublished observations). We focused our analysis on the muscle-expressed gene frm-4 (Roy et al., 2002), whose expression was decreased 2.8 fold in mef-2 mutants relative to wild type controls (p<0.001, Moderated t-statistic). Although frm-4 may not represent a direct transcriptional target of MEF-2, the abundance of its transcript provides a molecular assay for MEF-2 activity.

To determine if MEF-2 transcriptional activity is regulated by LevRs, we analyzed the abundance of frm-4 transcripts following a one hour application of levamisole. Levamisole treatment increased frm-4 mRNA abundance in wild-type animals but had no effect in unc-29 mutants (Fig. 7A), demonstrating that the increased frm-4 expression was not caused by a non-specific effect of drug treatment. By contrast, following levamisole treatment of mef-2 mutants, frm-4 expression was not increased but was instead significantly decreased. These results suggest that activation of LevRs induces the transcriptional activity of MEF-2, and likely other transcription factors.

Figure 7
Acute activation of LevRs initiates MEF-2-dependent changes in transcription and a retrograde change in YFP::RAB-3. After 1 hour, Levamisole (200µM) and mock treated animals were subjected to RNA extraction or imaging. A) Expression of frm-4 was ...

Levamisole treatment induces the MEF-2-dependent increase in RAB-3

If increased muscle activity, caused by levamisole treatment, induces the transcriptional activity of MEF-2, then we would expect that levamisole treatment would also induce the MEF-2-dependent retrograde signal. Consistent with this idea, we found that the pre-synaptic abundance of YFP::RAB-3 was significantly increased following treatment with levamisole (Fig. 7B,C,J). We did several controls to determine if this effect was specific for activation of LevRs. First, treatment with nicotine, which activates ACR-16 receptors (Francis et al., 2005), did not alter pre-synaptic RAB-3 fluorescence (Fig. 7P,Q). Second, the levamisole effect was dependent upon the activity of endogenous LevRs, as levamisole treatment of unc-29 mutants did not alter RAB-3 fluorescence (Fig 7D,E,J). Third, this effect also required MEF-2 activity in muscles, since levamisole treatment of mef-2 mutants also failed to alter RAB-3 levels, but transgenes driving MEF-2 expression in body muscles restored levamisole-induced increases in RAB-3 (Fig. 7F–J). Thus, acute activation of LevRs induces both MEF-2 transcriptional activity and the retrograde message, whereas acute activation of ACR-16 receptors (with nicotine) did not.

If LevR activity is required to induce the retrograde signal, we would also expect mutations inactivating these receptors would block the pre-synaptic effects of miR-1. Consistent with this idea, the effect of the mir-1 mutation on pre-synaptic RAB-3 levels was eliminated in mir-1 unc-29 double mutants (Fig. 7K–O). These results demonstrate that the activity of LevRs plays a pivotal role in regulating the activity of the retrograde message, mediating both acute induction following levamisole treatment and chronic induction in mir-1 mutants.


We show that the conserved microRNA miR-1 acts in C. elegans body muscle where it regulates both the sensitivity of muscle to ACh and the amount of ACh released from pre-synaptic neurons, via a retrograde messenger. miR-1 adjusts muscle sensitivity by directly regulating mRNAs encoding two subunits of the LevR (UNC-29 and UNC-63), whereas miR-1 adjusts pre-synaptic properties by regulating the mRNA encoding the transcription factor MEF-2. Acute activation of the LevR induces MEF-2-dependent transcription and the production of a MEF-2-dependent retrograde message. These results suggest that miR-1, LevRs, and MEF-2 define a nicotinic signaling pathway that couples post-synaptic activity with changes in pre-synaptic properties (Fig. 7R).

A new function for miR-1

Fly and mouse miR-1 knockouts suffer early lethality due to defects in muscle proliferation (Sokol and Ambros, 2005; Zhao et al., 2007) whereas C. elegans mir-1 mutants are viable, and muscle develops normally. There are several potential explanations for this discrepancy. First, worm muscle precursors do not undergo extensive proliferation prior to myogenic differentiation; consequently, the proliferative defects observed in mouse and fly would not be expected to occur in worm mir-1 mutants. Second, muscle differentiation in the worm differs from other metazoans in several ways. Worm body muscle precursors do not undergo cell fusions, instead forming mature mononucleate muscle cells. Third, several genes that are critical for normal myogenic development in other organisms (e.g. MEF-2 and MyoD) play no apparent role in the worm (Chen et al., 1992; Dichoso et al., 2000). These differences may explain why muscle development occurs relatively normally in mir-1 mutants.

In general, microRNAs have been described to regulate early developmental processes. Given that muscles develop normally in mir-1 mutants, we were able to identify novel functions for miR-1 in the regulation of mature neuromuscular synapses. Based on our results, we speculate that some microRNAs (like miR-1) will regulate both early developmental processes as well as the mature function of cells after development is completed. Consistent with a post-developmental function elsewhere, miR-1 expression persists through adulthood in both fly and mouse (Nguyen and Frasch, 2006).

microRNA regulation of subunit diversity in heteromultimeric receptors

Transcriptional regulation is thought to underlie changes in subunit composition of heteromultimeric receptors. For example, in vertebrates, nAChRs undergo a developmentally programmed switch from the γ to ε subunits that is mediated by changes in transcription of these genes (Gu and Hall, 1988; Missias et al., 1996).

We propose that miR-1 provides an alternative post-transcriptional mechanism to regulate the subunit composition of nAChRs. In mir-1 mutants, the abundance of targeted subunits (UNC-29 and UNC-63) was increased and this was accompanied by decreased muscle sensitivity to ACh. These changes in muscle sensitivity are mediated in part by altered composition of LevRs; however, other aspects of the post-synaptic phenotype (e.g. the change in endogenous EPSC amplitude and kinetics) are mediated by other miR-1 targets (e.g. MEF-2).

The precise mechanism by which LevR function is altered by miR-1 remains unclear. These effects could arise from changes in several aspects of receptor biogenesis and function, including association with accessory subunits, assembly in the ER, trafficking to the cell surface, or function of mature receptors on the muscle surface. In principle, any of these receptor properties could be modified by altered subunit composition.

Based on our results, we speculate that microRNAs may be utilized to regulate other heteromultimeric receptor complexes in an analogous manner. Subunit composition is known to regulate the signaling properties of many classes of receptors, e.g. growth factor receptors and cytokine receptors. Regulation by microRNAs could provide a novel mechanism to rapidly alter the composition and signaling properties of these receptors.

MEF-2 mediates the generation of a retrograde synaptic signal

Retrograde signaling has been proposed as a mechanism to adjust pre-synaptic release to match post-synaptic excitability during development and ongoing synaptic activity. This phenomenon has been extensively studied at the Drosophila NMJ where a variety of post-synaptic disruptions each produce a retrograde signal to increase neurotransmitter release (Davis and Bezprozvanny, 2001). In C. elegans, genetic data suggest the presence of a retrograde message at synapses, however thus far the nature of this message remains unknown (Doi and Iwasaki, 2002). We show here that MEF-2 activity in body muscles induces a retrograde signal that inhibits pre-synaptic release of ACh.

One puzzling aspect of our results is the phenotype of mef-2 single mutants. Since mef-2 mutations eliminate the retrograde signal, one might expect that mef-2 mutants would have increased ACh release; however, we observed decreased ACh secretion in mef-2 single mutants. Several mechanisms could explain this discrepancy. First, the decreased ACh release could be caused by MEF-2 functions unrelated to retrograde signaling. Second, MEF-2 and the retrograde signal may not be active under normal growth conditions. It seems likely that a homeostatic pathway would only be induced following periods of unusually high activity, and consequently may be inactive under normal growth conditions. Third, in the absence of an inducing signal, Mef2 actively represses expression of some target genes (Shalizi and Bonni, 2005; van der Linden et al., 2007). Thus, some MEF-2 targets will be de-repressed in mef-2 mutants. Consequently, mef-2 loss of function mutations may result in a weak induction of the retrograde signal. Consistent with this idea, we observed decreased ACh secretion and increased RAB-3 fluorescence in mef-2 mutants, both of which suggest an increase in retrograde signaling. Further experiments are required to distinguish between these possibilities.

Mammalian Mef2 proteins were previously implicated in synapse formation. RNAi mediated knockdown of rat Mef2A increased the number of excitatory synapses formed onto cerebellar and hippocampal neurons (Flavell et al., 2006; Shalizi et al., 2006). These results were interpreted as Mef2-mediated regulation of either synapse formation or stability. Thus, in both worm and mammalian studies, post-synaptic Mef2 regulates aspects of synapse development or function.

Although the mechanisms leading to Mef2 dependent changes in synapse formation in rat neurons have not been defined, it seems possible that retrograde regulation of pre-synaptic function may be involved in this process. Synapse refinement during development often involves competition among synaptic inputs, whereby weaker inputs are eliminated (Katz and Shatz, 1996; Sanes and Lichtman, 2001). We speculate that Mef2 effects on synapse formation in hippocampal neurons may be mediated by retrograde inhibition of synaptic inputs, leading to their elimination.

RAB-3 as an effector of retrograde signaling

Although we have not identified the MEF-2 regulated retrograde messenger, our results suggest that RAB-3 is the downstream target of this activity. Pre-synaptic RAB-3 levels were increased when retrograde signaling was induced (i.e. in mir-1 mutants or following levamisole treatment) and these effects were dependent on MEF-2 activity in muscles. Moreover, the ACh secretion defects caused by mir-1 and rab-3 mutations were strikingly similar, and additive defects were not observed in double mutants. Together these results strongly support the idea that the miR-1/MEF-2 regulated retrograde messenger acts via changes in pre-synaptic RAB-3 activity.

What is the mechanism underlying the secretion defects in mir-1 mutants? Genetic studies in mice suggest that Rab3 proteins act at a late stage in the synaptic vesicle cycle, after docking and priming, to promote calcium-evoked fusion (Schluter et al., 2004). Our analysis of mir-1 mutants is entirely consistent with this phenotype. We observed decreased stimulus evoked release, and a decreased rate of endogenous EPSCs, whereas there was no change in vesicle priming, nor in the number of primed vesicles available for release. Together these results suggest that vesicle release probability was reduced in mir-1 mutants, as previously proposed for mice lacking Rab3 function (Schluter et al., 2004). Decreased vesicular release probability could be caused by decreased calcium influx, or by decreased calcium sensitivity of release. Given the strong conservation of RAB-3 and MEF-2 function, it seems likely that Rab3 may also be a pre-synaptic effector responsible for retrograde signaling by Mef2 in mammalian neurons, and perhaps for other retrograde messengers.

Regulation of retrograde signaling by muscle activity

Several results suggest that the LevR/MEF-2 retrograde signal is induced by muscle activity. For example, activation of muscle LevRs induces MEF-2 transcriptional activity and the MEF-2 mediated retrograde alteration of presynaptic RAB-3. By contrast, activation of muscle ACR-16 receptors with nicotine did not induce the retrograde alteration of RAB-3. Thus, LevRs are selectively coupled to MEF-2 function, providing a mechanism to couple muscle depolarization to the generation of a retrograde message.

What confers activity-dependence on the retrograde message? Dephosphorylation of Mef2 induces its transcriptional activity, and this dephosphorylation is often mediated by the calcium-activated phosphatase calcineurin (Flavell et al., 2006; Shalizi et al., 2006; Shalizi and Bonni, 2005). The calcium-dependence of MEF-2 activity could account for the activity dependence of the retrograde message. Consistent with this idea, LevRs isolated from worm extracts co-purify with the C. elegans ortholog of calcineurin, TAX-6 (Gottschalk et al., 2005). Roughly 50% of the current produced by muscle LevRs is mediated by calcium (Richmond and Jorgensen, 1999). We propose that association of calcineurin with LevRs provides a mechanism to selectively couple activation of LevRs to induction of MEF-2 transcriptional activity and retrograde signaling.

Feedback inhibition by homeostatic plasticity is thought to provide a mechanism to maintain consistent synaptic function in the face of fluctuations in activity (Davis and Bezprozvanny, 2001; Turrigiano and Nelson, 2004). We propose that LevR/MEF-2 retrograde signal identified here is an example of such a homeostatic pathway. In this scenario (Fig. 7R), during periods of increased activity, synaptic currents through the LevR would activate MEF-2, which in turn would initiate a retrograde message culminating in inactivation of RAB-3 and decreased ACh secretion. Thus, this pathway provides a mechanism to couple muscle depolarization to homeostatic inhibition of excitatory input.

We also show that miR-1 regulates multiple aspects of retrograde signaling. The subunit composition and function of LevRs, and the expression of MEF-2 are regulated by miR-1, all of which would regulate induction of the retrograde signal. Consistent with these regulatory effects, retrograde signaling was constitutively active in mir-1 mutants. These results suggest that miR-1 regulates the gain of this homeostatic signal. When miR-1 activity is high, the threshold for inducing the retrograde signal would be shifted to higher levels of muscle activity, whereas the converse would be true when miR-1 activity is low. Further experiments are required to determine which specific physiological conditions, or when during development miR-1 levels or activity are altered. Our results suggest that miR-1 provides a potential mechanism to adjust the intensity of retrograde signaling.

Materials and Methods

Detailed descriptions of all methods are provided in the on-line Supplemental Information.

Strains were maintained at 20°C on lawns of OP50 (for imaging and behavior) or HB101 (for electrophysiology). A complete list of strains utilized is provided in the Supplemental Methods.

Western Blots and Immunofluorescence

Membranes were isolated from worm extracts by ultracentrifugation, solublized in sample buffer, and immunoblotted as described previously (Dreier et al., 2005). Custom polyclonal antibodies were raised against recombinant UNC-29, UNC-63, and UNC-38 GST-fusion proteins. Commercial anti-GFP antibody (Clontech) was utilized. For whole mount immunostaining, worms were fixed with Bouin’s and processed as described previously (McEwen et al., 2006).


All expression vectors are based on the pPD49.26 backbone (A. Fire). Standard methods were utilized to construct all plasmids. Full details will be provided upon request. A 2.4kb myo-3 promoter was used for expression in body muscles. nAChR and mef-2 cDNAs were isolated by RT-PCR. For 3’UTR reporter constructs, a myristoylated GFP was cloned into pPD49.26 followed by 3’UTR sequences derived from unc-29 (KP#1299), unc-38 (KP#1355), or mef-2 (KP#1394). pJK129 contains GFP fused to a mutagenized unc-29 3’UTR, in which the nucleotide sequences of the three miR-1 binding sites were randomized.

Drug treatment assays

Young adult worms were transferred to plates containing 200µM levamisole and assayed for paralysis as described previously (Nurrish et al., 1999). For imaging and real-time PCR experiments, late L4 worms were transferred to mock treatment plates or plates containing 200uM levamisole or 30mM nicotine. After one hour RNA was harvested or worms were immobilized for imaging.


Electrophysiology was done on dissected C. elegans as previously described (Richmond and Jorgensen, 1999). All recording conditions, data acquisition, and analysis were as described (McEwen et al., 2006). Data analysis was carried out in Igor Pro using custom written software. Stimulus artifacts for stimulus evoked responses were removed for clarity. The on-line supplement contains a detailed description of our conditions and analysis.


Quantitative wide field fluorescence microscopy was performed using custom software as described previously (Dittman and Kaplan, 2006).

Real-Time PCR

Total RNA was extracted from synchronized L4 worms and reverse transcribed (RetroScript, Ambion), according to the manufacturer’s suggestions. Quantitative Real-Time PCR (qPCR) reactions were performed using SYBR Green Supermix (BioRad) using a BioRad iCycler IQ. Relative abundance of frm-4 and rpl-32 mRNAs was determined using gene specific primers (sequences provided upon request).

Supplementary Material



We thank the following for strains, advice, reagents, and comments on the manuscript: C. elegans stock center, S. Mitani, M. Nonet, L. Dreier, S. Curran, D. Sieburth, Q. Ch’ng, J.B. Cohen, and members of the Kaplan and Ruvkun labs. We thank M. Greenberg and S. Flavell for sharing unpublished data and for helpful discussions. This work was supported by postdoctoral fellowships from the NIH (1F32NS10310) and the American Cancer Society (PF-98-065-01-DDC), and the Massachusetts General Hospital ECOR Fund for Medical Discovery (J.M.) and by research grants from the NIH (J.K.)


Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.


  • Black B, Lu J, Olson E. The MEF2A 3′ untranslated region functions as a cis-acting translational repressor. Mol Cell Biol. 1997;17:2756–2763. [PMC free article] [PubMed]
  • Brown LA, Jones AK, Buckingham SD, Mee CJ, Sattelle DB. Contributions from Caenorhabditis elegans functional genetics to antiparasitic drug target identification and validation: nicotinic acetylcholine receptors, a case study. Int J Parasitol. 2006;36:617–624. [PubMed]
  • Cao X, Yeo G, Muotri AR, Kuwabara T, Gage FH. NONCODING RNAS IN THE MAMMALIAN CENTRAL NERVOUS SYSTEM. Annual Review of Neuroscience. 2006;29:77–103. [PubMed]
  • Chen L, Krause M, Draper B, Weintraub H, Fire A. Body-wall muscle formation in Caenorhabditis elegans embryos that lack the MyoD homolog hlh-1. Science. 1992;256:240–243. [PubMed]
  • Davis GW, Bezprozvanny I. MAINTAINING THE STABILITY OF NEURAL FUNCTION: A Homeostatic Hypothesis. Annual Review of Physiology. 2001;63:847–869. [PubMed]
  • Dichoso D, Brodigan T, Chwoe KY, Lee JS, Llacer R, Park M, Corsi AK, Kostas SA, Fire A, Ahnn J, Krause M. The MADS-Box Factor CeMEF2 Is Not Essential for Caenorhabditis elegans Myogenesis and Development. Developmental Biology. 2000;223:431–440. [PubMed]
  • Dittman JS, Kaplan JM. Factors regulating the abundance and localization of synaptobrevin in the plasma membrane. PNAS. 2006;103:11399–11404. [PubMed]
  • Doi M, Iwasaki K. Regulation of Retrograde Signaling at Neuromuscular Junctions by the Novel C2 Domain Protein AEX-1. Neuron. 2002;33:249–259. [PubMed]
  • Dreier L, Burbea M, Kaplan JM. LIN-23-Mediated Degradation of [beta]-Catenin Regulates the Abundance of GLR-1 Glutamate Receptors in the Ventral Nerve Cord of C. elegans. Neuron. 2005;46:51–64. [PubMed]
  • Enright A, John B, Gaul U, Tuschl T, Sander C, Marks D. MicroRNA targets in Drosophila. Genome Biology. 2003;5:R1. [PMC free article] [PubMed]
  • Flavell SW, Cowan CW, Kim T-K, Greer PL, Lin Y, Paradis S, Griffith EC, Hu LS, Chen C, Greenberg ME. Activity-Dependent Regulation of MEF2 Transcription Factors Suppresses Excitatory Synapse Number. Science. 2006;311:1008–1012. [PubMed]
  • Francis MM, Evans SP, Jensen M, Madsen DM, Mancuso J, Norman KR, Maricq AV. The Ror Receptor Tyrosine Kinase CAM-1 Is Required for ACR-16-Mediated Synaptic Transmission at the C. elegans Neuromuscular Junction. Neuron. 2005;46:581–594. [PubMed]
  • Giraldez AJ, Cinalli RM, Glasner ME, Enright AJ, Thomson JM, Baskerville S, Hammond SM, Bartel DP, Schier AF. MicroRNAs Regulate Brain Morphogenesis in Zebrafish. Science. 2005;308:833–838. [PubMed]
  • Gottschalk A, Almedom RB, Schedletzky T, Anderson SD, Yates JR, 3rd, Schafer WR. Identification and characterization of novel nicotinic receptor-associated proteins in Caenorhabditis elegans. Embo J. 2005;24:2566–2578. [PubMed]
  • Griffiths-Jones S, Grocock RJ, van Dongen S, Bateman A, Enright AJ. miRBase: microRNA sequences, targets and gene nomenclature. Nucl Acids Res. 2006;34:D140–D144. [PMC free article] [PubMed]
  • Gu Y, Hall ZW. Immunological evidence for a change in subunits of the acetylcholine receptor in developing and denervated rat muscle. Neuron. 1988;1:117–125. [PubMed]
  • Johnston RJ, Hobert O. A microRNA controlling left/right neuronal asymmetry in Caenorhabditis elegans. Nature. 2003;426:845–849. [PubMed]
  • Kalinovsky A, Scheiffele P. Transcriptional control of synaptic differentiation by retrograde signals. Current Opinion in Neurobiology. 2004;14:272–279. [PubMed]
  • Katz LC, Shatz CJ. Synaptic Activity and the Construction of Cortical Circuits. Science. 1996;274:1133–1138. [PubMed]
  • Kosik KS. The neuronal microRNA system. Nat Rev Neurosci. 2006;7:911–920. [PubMed]
  • Koushika SP, Richmond JE, Hadwiger G, Weimer RM, Jorgensen EM, Nonet ML. A post-docking role for active zone protein Rim. Nat Neurosci. 2001;4:997–1005. [PMC free article] [PubMed]
  • Lall S, Grun D, Krek A, Chen K, Wang Y-L, Dewey CN, Sood P, Colombo T, Bray N, MacMenamin P. A Genome-Wide Map of Conserved MicroRNA Targets in C. elegans. Current Biology. 2006;16:460–471. [PubMed]
  • Lee RC, Ambros V. An Extensive Class of Small RNAs in Caenorhabditis elegans. Science. 2001;294:862–864. [PubMed]
  • McEwen JM, Madison JM, Dybbs M, Kaplan JM. Antagonistic Regulation of Synaptic Vesicle Priming by Tomosyn and UNC-13. Neuron. 2006;51:303–315. [PubMed]
  • Millar NS. Assembly and subunit diversity of nicotinic acetylcholine receptors. Biochem Soc Trans. 2003;31:869–874. [PubMed]
  • Miller KG, Alfonso A, Nguyen M, Crowell JA, Johnson CD, Rand JB. A genetic selection for Caenorhabditis elegans synaptic transmission mutants 10.1073/pnas.93.22.12593. Proceedings of the National Academy of Sciences. 1996;93:12593–12598. [PubMed]
  • Missias AC, Chu GC, Klocke BJ, Sanes JR, Merlie JP. Maturation of the Acetylcholine Receptor in Skeletal Muscle: Regulation of the AChR [gamma]-to-[epsilon] Switch. Developmental Biology. 1996;179:223–238. [PubMed]
  • Nguyen HT, Frasch M. MicroRNAs in muscle differentiation: lessons from Drosophila and beyond. Current Opinion in Genetics & Development. 2006;16:533–539. [PubMed]
  • Nurrish S, Segalat L, Kaplan JM. Serotonin Inhibition of Synaptic Transmission: G[alpha]o Decreases the Abundance of UNC-13 at Release Sites. Neuron. 1999;24:231–242. [PubMed]
  • Rao PK, Kumar RM, Farkhondeh M, Baskerville S, Lodish HF. Myogenic factors that regulate expression of muscle-specific microRNAs. PNAS. 2006;103:8721–8726. [PubMed]
  • Richmond JE, Jorgensen EM. One GABA and two acetylcholine receptors function at the C. elegans neuromuscular junction. Nature Neuroscience. 1999;2:791–797. [PMC free article] [PubMed]
  • Rosenmund C, Stevens CF. Definition of the Readily Releasable Pool of Vesicles at Hippocampal Synapses. Neuron. 1996;16:1197–1207. [PubMed]
  • Roy PJ, Stuart JM, Lund J, Kim SK. Chromosomal clustering of muscle-expressed genes in Caenorhabditis elegans. Nature. 2002;418:975–979. [PubMed]
  • Scheiffele P. CELL-CELL SIGNALING DURING SYNAPSE FORMATION IN THE CNS. Annual Review of Neuroscience. 2003;26:485–508. [PubMed]
  • Schluter OM, Schmitz F, Jahn R, Rosenmund C, Sudhof TC. A Complete Genetic Analysis of Neuronal Rab3 Function. J Neurosci. 2004;24:6629–6637. [PubMed]
  • Schratt GM, Tuebing F, Nigh EA, Kane CG, Sabatini ME, Kiebler M, Greenberg ME. A brain-specific microRNA regulates dendritic spine development. Nature. 2006;439:283–289. [PubMed]
  • Shalizi A, Gaudilliere B, Yuan Z, Stegmuller J, Shirogane T, Ge Q, Tan Y, Schulman B, Harper JW, Bonni A. A Calcium-Regulated MEF2 Sumoylation Switch Controls Postsynaptic Differentiation. Science. 2006;311:1012–1017. [PubMed]
  • Shalizi AK, Bonni A. Brawn for Brains: The Role of MEF2 Proteins in the Developing Nervous System. Curr Top Dev Biol. 2005;69:239–266. [PubMed]
  • Sokol NS, Ambros V. Mesodermally expressed Drosophila microRNA-1 is regulated by Twist and is required in muscles during larval growth. Genes Dev. 2005;19:2343–2354. [PubMed]
  • Sudhof TC. The Synaptic Vesicle Cycle. Annual Review of Neuroscience. 2004;27:509–547. [PubMed]
  • Thiagarajan R, Tewolde T, Li Y, Becker PL, Rich MM, Engisch KL. Rab3A negatively regulates activity-dependent modulation of exocytosis in bovine adrenal chromaffin cells. J Physiol. 2004;555:439–457. [PubMed]
  • Touroutine D, Fox RM, Von Stetina SE, Burdina A, Miller DM, III, Richmond JE. acr-16 Encodes an Essential Subunit of the Levamisole-resistant Nicotinic Receptor at the Caenorhabditis elegans Neuromuscular Junction. J Biol Chem. 2005;280:27013–27021. [PubMed]
  • Turrigiano GG, Nelson SB. Homeostatic plasticity in the developing nervous system. Nat Rev Neurosci. 2004;5:97–107. [PubMed]
  • van der Linden AM, Nolan KM, Sengupta P. KIN-29 SIK regulates chemoreceptor gene expression via an MEF2 transcription factor and a class II HDAC. Embo J. 2007;26:358–370. [PubMed]
  • Zhao Y, Ransom JF, Li A, Vedantham V, von Drehle M, Muth AN, Tsuchihashi T, McManus MT, Schwartz RJ, Srivastava D. Dysregulation of Cardiogenesis, Cardiac Conduction, and Cell Cycle in Mice Lacking miRNA-1-2. Cell. 2007;129:303–317. [PubMed]
  • Zhao Y, Samal E, Srivastava D. Serum response factor regulates a muscle-specific microRNA that targets Hand2 during cardiogenesis. Nature. 2005;436:214–220. [PubMed]
  • Zhen M, Jin Y. The liprin protein SYD-2 regulates the differentiation of presynaptic termini in C. elegans. Nature. 1999;401:371–375. [PubMed]