PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
 
J Virol. 1987 April; 61(4): 1061–1066.
PMCID: PMC254063

Transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 DNA.

Abstract

Human keratinocytes and fibroblasts isolated from foreskin were transformed by transfection with recombinant human papillomavirus type 16 (HPV16) DNA. The transformed cells exhibited an extended (fibroblasts) or indefinite (keratinocytes) life-span compared with that of normal controls. In addition, HS27, a human fibroblast cell line previously transfected with origin-defective simian virus 40, was successfully transfected. HPV16 sequences were stably maintained in the cells, and extensive amplification and rearrangements occurred with continuous culturing. Moreover, both fibroblasts and keratinocytes expressed several specific HPV16 mRNAs. Because HPV16-transfected cells had viral transcripts and because transfection with the vector alone did not extend the life-span of the cells, it is likely that the virus was responsible for the indefinite life-span. Transfected fibroblast and keratinocyte lines will be useful for investigating the molecular biology of HPV16 and the interactions between the viral DNA and the human genome. Moreover, transfected keratinocytes provide a model for analyzing the effects of HPV16 on the differentiation properties of human epithelial cells.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Burnett TS, Gallimore PH. Establishment of a human keratinocyte cell line carrying complete human papillomavirus type 1 genomes: lack of vegetative viral DNA synthesis upon keratinization. J Gen Virol. 1983 Jul;64(Pt 7):1509–1520. [PubMed]
  • Chesters PM, McCance DJ. Human papillomavirus type 16 recombinant DNA is maintained as an autonomously replicating episome in monkey kidney cells. J Gen Virol. 1985 Mar;66(Pt 3):615–620. [PubMed]
  • Crum CP, Mitao M, Levine RU, Silverstein S. Cervical papillomaviruses segregate within morphologically distinct precancerous lesions. J Virol. 1985 Jun;54(3):675–681. [PMC free article] [PubMed]
  • DiPaolo JA. Relative difficulties in transforming human and animal cells in vitro. J Natl Cancer Inst. 1983 Jan;70(1):3–8. [PubMed]
  • Dürst M, Gissmann L, Ikenberg H, zur Hausen H. A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3812–3815. [PubMed]
  • Dürst M, Kleinheinz A, Hotz M, Gissmann L. The physical state of human papillomavirus type 16 DNA in benign and malignant genital tumours. J Gen Virol. 1985 Jul;66(Pt 7):1515–1522. [PubMed]
  • Franceschi S, Doll R, Gallwey J, La Vecchia C, Peto R, Spriggs AI. Genital warts and cervical neoplasia: an epidemiological study. Br J Cancer. 1983 Nov;48(5):621–628. [PubMed]
  • Kreider JW, Howett MK, Wolfe SA, Bartlett GL, Zaino RJ, Sedlacek T, Mortel R. Morphological transformation in vivo of human uterine cervix with papillomavirus from condylomata acuminata. Nature. 1985 Oct 17;317(6038):639–641. [PubMed]
  • LaPorta RF, Taichman LB. Human papilloma viral DNA replicates as a stable episome in cultured epidermal keratinocytes. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3393–3397. [PubMed]
  • Law MF, Byrne JC, Howley PM. A stable bovine papillomavirus hybrid plasmid that expresses a dominant selective trait. Mol Cell Biol. 1983 Nov;3(11):2110–2115. [PMC free article] [PubMed]
  • Lehn H, Ernst TM, Sauer G. Transcription of episomal papillomavirus DNA in human condylomata acuminata and Buschke-Löwenstein tumours. J Gen Virol. 1984 Nov;65(Pt 11):2003–2010. [PubMed]
  • Lehn H, Krieg P, Sauer G. Papillomavirus genomes in human cervical tumors: analysis of their transcriptional activity. Proc Natl Acad Sci U S A. 1985 Aug;82(16):5540–5544. [PubMed]
  • McCance DJ. Human papillomaviruses and cancer. Biochim Biophys Acta. 1986;823(3):195–205. [PubMed]
  • Murnane JP, Fuller LF, Painter RB. Establishment and characterization of a permanent pSV ori--transformed ataxia-telangiectasia cell line. Exp Cell Res. 1985 May;158(1):119–126. [PubMed]
  • Pfister H. Biology and biochemistry of papillomaviruses. Rev Physiol Biochem Pharmacol. 1984;99:111–181. [PubMed]
  • Popescu NC, Amsbaugh SC, Milo G, DiPaolo JA. Chromosome alterations associated with in vitro exposure of human fibroblasts to chemical or physical carcinogens. Cancer Res. 1986 Sep;46(9):4720–4725. [PubMed]
  • Schneider A, Kraus H, Schuhmann R, Gissmann L. Papillomavirus infection of the lower genital tract: detection of viral DNA in gynecological swabs. Int J Cancer. 1985 Apr 15;35(4):443–448. [PubMed]
  • Scholl SM, Pillers EM, Robinson RE, Farrell PJ. Prevalence of human papillomavirus type 16 DNA in cervical carcinoma samples in East Anglia. Int J Cancer. 1985 Feb 15;35(2):215–218. [PubMed]
  • Schwarz E, Freese UK, Gissmann L, Mayer W, Roggenbuck B, Stremlau A, zur Hausen H. Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature. 1985 Mar 7;314(6006):111–114. [PubMed]
  • Seedorf K, Krämmer G, Dürst M, Suhai S, Röwekamp WG. Human papillomavirus type 16 DNA sequence. Virology. 1985 Aug;145(1):181–185. [PubMed]
  • Trevathan E, Layde P, Webster LA, Adams JB, Benigno BB, Ory H. Cigarette smoking and dysplasia and carcinoma in situ of the uterine cervix. JAMA. 1983 Jul 22;250(4):499–502. [PubMed]
  • Tsunokawa Y, Takebe N, Kasamatsu T, Terada M, Sugimura T. Transforming activity of human papillomavirus type 16 DNA sequence in a cervical cancer. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2200–2203. [PubMed]
  • Wigler M, Pellicer A, Silverstein S, Axel R, Urlaub G, Chasin L. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373–1376. [PubMed]
  • Yasumoto S, Burkhardt AL, Doniger J, DiPaolo JA. Human papillomavirus type 16 DNA-induced malignant transformation of NIH 3T3 cells. J Virol. 1986 Feb;57(2):572–577. [PMC free article] [PubMed]
  • Yee C, Krishnan-Hewlett I, Baker CC, Schlegel R, Howley PM. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am J Pathol. 1985 Jun;119(3):361–366. [PubMed]
  • Yoshikawa H, Matsukura T, Yamamoto E, Kawana T, Mizuno M, Yoshiike K. Occurrence of human papillomavirus types 16 and 18 DNA in cervical carcinomas from Japan: age of patients and histological type of carcinomas. Jpn J Cancer Res. 1985 Aug;76(8):667–671. [PubMed]
  • zur Hausen H. Human papillomaviruses and their possible role in squamous cell carcinomas. Curr Top Microbiol Immunol. 1977;78:1–30. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)