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J Virol. 1986 December; 60(3): 1068–1074.
PMCID: PMC253347

Direct inactivation of viruses by human granulocyte defensins.


Human neutrophils contain a family of microbicidal peptides known as defensins. One of these defensins, human neutrophil peptide (HNP)-1, was purified, and its ability to directly inactivate several viruses was extensively tested. Herpes simplex virus (HSV) types 1 and 2, cytomegalovirus, vesicular stomatitis virus, and influenza virus A/WSN were inactivated by incubation with HNP-1. Two nonenveloped viruses, echovirus type 11 and reovirus type 3, were resistant to inactivation. Purified homologous peptides HNP-2 and HNP-3 were found to have HSV-1-neutralizing activities approximately equal to that of HNP-1. Inactivation of HSV-1 by HNP-1 depended on the time, temperature, and pH of incubation. Antiviral activity was abrogated by low temperature or prior reduction and alkylation of the defensins. Addition of serum or serum albumin to the incubation mixtures inhibited neutralization of HSV-1 by HNP-1. We used density gradient sedimentation techniques to demonstrate that HNP-1 bound to HSV-1 in a temperature-dependent manner. We speculate that binding of defensin peptides to certain viruses may impair their ability to infect cells.

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Selected References

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  • Allison AC. Interactions of antibodies, complement components and various cell types in immunity against viruses and pyogenic bacteria. Transplant Rev. 1974;19(0):3–55. [PubMed]
  • Babior BM. Oxygen-dependent microbial killing by phagocytes (first of two parts). N Engl J Med. 1978 Mar 23;298(12):659–668. [PubMed]
  • Berkow RL, Baehner RL. Volume-dependent human blood polymorphonuclear leukocyte heterogeneity demonstrated with counterflow centrifugal elutriation. Blood. 1985 Jan;65(1):71–78. [PubMed]
  • Elsbach P, Weiss J. A reevaluation of the roles of the O2-dependent and O2-independent microbicidal systems of phagocytes. Rev Infect Dis. 1983 Sep-Oct;5(5):843–853. [PubMed]
  • Ganz T, Selsted ME, Lehrer RI. Antimicrobial activity of phagocyte granule proteins. Semin Respir Infect. 1986 Jun;1(2):107–117. [PubMed]
  • Ganz T, Selsted ME, Szklarek D, Harwig SS, Daher K, Bainton DF, Lehrer RI. Defensins. Natural peptide antibiotics of human neutrophils. J Clin Invest. 1985 Oct;76(4):1427–1435. [PMC free article] [PubMed]
  • Grewal AS, Rouse BT. Destruction of virus infected cells by neutrophils and complement. Experientia. 1980 Mar 15;36(3):352–354. [PubMed]
  • Grewal AS, Rouse BT, Babiuk LA. Mechanisms of resistant of herpesviruses: comparison of the effectiveness of different cell types in mediating antibody-dependent cell-mediated cytotoxicity. Infect Immun. 1977 Mar;15(3):698–703. [PMC free article] [PubMed]
  • Hill TJ, Field HJ, Blyth WA. Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. J Gen Virol. 1975 Sep;28(3):341–353. [PubMed]
  • Jordan GW, Seet EC. Antiviral effects of amphotericin B methyl ester. Antimicrob Agents Chemother. 1978 Feb;13(2):199–204. [PMC free article] [PubMed]
  • Lehrer RI, Daher K, Ganz T, Selsted ME. Direct inactivation of viruses by MCP-1 and MCP-2, natural peptide antibiotics from rabbit leukocytes. J Virol. 1985 May;54(2):467–472. [PMC free article] [PubMed]
  • Lehrer RI, Ladra KM, Hake RB. Nonoxidative fungicidal mechanisms of mammalian granulocytes: demonstration of components with candidacidal activity in human, rabbit, and guinea pig leukocytes. Infect Immun. 1975 Jun;11(6):1226–1234. [PMC free article] [PubMed]
  • Lehrer RI, Szklarek D, Ganz T, Selsted ME. Correlation of binding of rabbit granulocyte peptides to Candida albicans with candidacidal activity. Infect Immun. 1985 Jul;49(1):207–211. [PMC free article] [PubMed]
  • McSorley J, Shapiro L, Brownstein MH, Hsu KC. Herpes simplex and varicella-zoster: comparative histopathology of 77 cases. Int J Dermatol. 1974 Mar-Apr;13(2):69–75. [PubMed]
  • Oleske JM, Ashman RB, Kohl S, Shore SL, Starr SE, Wood P, Nahmias AJ. Human polymorphonuclear leucocytes as mediators of antibody-dependent cellular cytotoxicity to herpes simplex virus-infected cells. Clin Exp Immunol. 1977 Mar;27(3):446–453. [PubMed]
  • Panyim S, Chalkley R. High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys. 1969 Mar;130(1):337–346. [PubMed]
  • Rouse BT. Role of neutrophils in antiviral immunity. Adv Exp Med Biol. 1981;137:263–278. [PubMed]
  • Rouse BT, Babiuk LA, Henson PM. Neutrophils in antiviral immunity: inhibition of virus replication by a mediator produced by bovine neutrophils. J Infect Dis. 1980 Feb;141(2):223–232. [PubMed]
  • Rouse BT, Wardley RC, Babiuk LA, Mukkur TK. The role of neutrophils in antiviral defense--in vitro studies on the mechanism of antiviral inhibition. J Immunol. 1977 Jun;118(6):1957–1961. [PubMed]
  • Russell AS, Miller C. A possible role for polymorphonuclear leucocytes in the defence against recrudescent herpes simplex virus infection in man. Immunology. 1978 Mar;34(3):371–378. [PubMed]
  • Sands J, Auperin D, Snipes W. Extreme sensitivity of enveloped viruses, including herpes simplex, to long-chain unsaturated monoglycerides and alcohols. Antimicrob Agents Chemother. 1979 Jan;15(1):67–73. [PMC free article] [PubMed]
  • Selsted ME, Brown DM, DeLange RJ, Lehrer RI. Primary structures of MCP-1 and MCP-2, natural peptide antibiotics of rabbit lung macrophages. J Biol Chem. 1983 Dec 10;258(23):14485–14489. [PubMed]
  • Selsted ME, Harwig SS, Ganz T, Schilling JW, Lehrer RI. Primary structures of three human neutrophil defensins. J Clin Invest. 1985 Oct;76(4):1436–1439. [PMC free article] [PubMed]
  • Siebens H, Tevethia SS, Babior BM. Neutrophil-mediated antibody-dependent killing of herpes-simplex-virus-infected cells. Blood. 1979 Jul;54(1):88–94. [PubMed]
  • Spitznagel JK. Nonoxidative antimicrobial reactions of leukocytes. Contemp Top Immunobiol. 1984;14:283–343. [PubMed]
  • Stevens DA, Ferrington RA, Jordan GW, Merigan TC. Cellular events in zoster vesicles: relation to clinical course and immune parameters. J Infect Dis. 1975 May;131(5):509–515. [PubMed]

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