PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
 
J Virol. 1986 May; 58(2): 487–492.
PMCID: PMC252935

Characterization of the Snow Mountain agent of viral gastroenteritis.

Abstract

Snow Mountain agent (SMA) is a 27- to 32-nm virus which is the etiologic agent of outbreaks of acute gastroenteritis in Colorado and Vermont. SMA is morphologically similar to but antigenically distinct from the Norwalk and Hawaii agents of viral gastroenteritis but, like those agents, has not been cultivated in vitro. We purified and characterized SMA directly from human stool specimens containing the virus. The density of the SMA virion was 1.29 g/cm3 and 1.21 to 1.22 g/cm3 on potassium tartrate-glycerol gradients and 1.33 to 1.34 g/cm3 on cesium chloride gradients. SMA had an S value of 170 to 183S on a sucrose velocity gradient. The purified virion was iodinated, immunoprecipitated with acute and convalescent sera from volunteers challenged with SMA, and analyzed on polyacrylamide gels. The virion contains one major structural protein of 62,000 molecular weight, which is similar in size to the 59,000-molecular-weight protein found in the Norwalk virion. The biophysical properties and single structural protein of SMA most closely resemble those of the calicivirus group.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Appleton H, Buckley M, Thom BT, Cotton JL, Henderson S. Virus-like particles in winter vomiting disease. Lancet. 1977 Feb 19;1(8008):409–411. [PubMed]
  • Appleton H, Pereira MS. A possible virus aetiology in outbreaks of food-poisoning from cockles. Lancet. 1977 Apr 9;1(8015):780–781. [PubMed]
  • Ashley CR, Caul EO. Potassium tartrate-glycerol as a density gradient substrate for separation of small, round viruses from human feces. J Clin Microbiol. 1982 Aug;16(2):377–381. [PMC free article] [PubMed]
  • Acute infectious nonbacterial gastroenteritis: etiology and pathogenesis. Ann Intern Med. 1972 Jun;76(6):993–1008. [PubMed]
  • Brondum J, Spitalny KC, Vogt RL, Godlewski K, Madore HP, Dolin R. Snow Mountain agent associated with an outbreak of gastroenteritis in Vermont. J Infect Dis. 1985 Oct;152(4):834–837. [PubMed]
  • Clarke SK, Cook GT, Egglestone SI, Hall TS, Miller DL, Reed SE, Rubenstein D, Smith AJ, Tyrrell DA. A virus from epidemic vomiting disease. Br Med J. 1972 Jul 8;3(5818):86–89. [PMC free article] [PubMed]
  • Dolin R. Norwalk agent-like particles associated with gastroenteritis in human beings. J Am Vet Med Assoc. 1978 Sep 1;173(5 Pt 2):615–619. [PubMed]
  • Dolin R, Blacklow NR, DuPont H, Formal S, Buscho RF, Kasel JA, Chames RP, Hornick R, Chanock RM. Transmission of acute infectious nonbacterial gastroenteritis to volunteers by oral administration of stool filtrates. J Infect Dis. 1971 Mar;123(3):307–312. [PubMed]
  • Dolin R, Levy AG, Wyatt RG, Thornhill TS, Gardner JD. Viral gastroenteritis induced by the Hawaii agent. Jejunal histopathology and serologic response. Am J Med. 1975 Dec;59(6):761–768. [PubMed]
  • Dolin R, Reichman RC, Roessner KD, Tralka TS, Schooley RT, Gary W, Morens D. Detection by immune electron microscopy of the Snow Mountain agent of acute viral gastroenteritis. J Infect Dis. 1982 Aug;146(2):184–189. [PubMed]
  • Greenberg HB, Valdesuso JR, Kalica AR, Wyatt RG, McAuliffe VJ, Kapikian AZ, Chanock RM. Proteins of Norwalk virus. J Virol. 1981 Mar;37(3):994–999. [PMC free article] [PubMed]
  • Greenberg HB, Wyatt RG, Valdesuso J, Kalica AR, London WT, Chanock RM, Kapikian AZ. Solid-phase microtiter radioimmunoassay for detection of the Norwalk strain of acute nonbacterial, epidemic gastroenteritis virus and its antibodies. J Med Virol. 1978;2(2):97–108. [PubMed]
  • Kapikian AZ, Gerin JL, Wyatt RG, Thornhill TS, Chanock RM. Density in cesium chloride of the 27 nm "8FIIa" particle associated with acute infectious nonbacterial gastroenteritis: determination by ultra-centrifugation and immune electron microscopy. Proc Soc Exp Biol Med. 1973 Mar;142(3):874–877. [PubMed]
  • Kapikian AZ, Wyatt RG, Dolin R, Thornhill TS, Kalica AR, Chanock RM. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972 Nov;10(5):1075–1081. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Morens DM, Zweighaft RM, Vernon TM, Gary GW, Eslien JJ, Wood BT, Holman RC, Dolin R. A waterborne outbreak of gastroenteritis with secondary person-to-person spread. Association with a viral agent. Lancet. 1979 May 5;1(8123):964–966. [PubMed]
  • Oshiro LS, Haley CE, Roberto RR, Riggs JL, Croughan M, Greenberg H, Kapikian A. A 27-nm virus isolated during an outbreak of acute infectious nonbacterial gastroenteritis in a convalescent hospital: a possible new serotype. J Infect Dis. 1981 Jun;143(6):791–795. [PubMed]
  • Schaffer FL, Bachrach HL, Brown F, Gillespie JH, Burroughs JN, Madin SH, Madeley CR, Povey RC, Scott F, Smith AW, et al. Caliciviridae. Intervirology. 1980;14(1):1–6. [PubMed]
  • Swanstrom R, Shank PR. X-Ray Intensifying Screens Greatly Enhance the Detection by Autoradiography of the Radioactive Isotopes 32P and 125I. Anal Biochem. 1978 May;86(1):184–192. [PubMed]
  • Terashima H, Chiba S, Sakuma Y, Kogasaka R, Nakata S, Minami R, Horino K, Nakao T. The polypeptide of a human calicivirus. Arch Virol. 1983;78(1-2):1–7. [PubMed]
  • Thornhill TS, Wyatt RG, Kalica AR, Dolin R, Chanock RM, Kapikian AZ. Detection by immune electron microscopy of 26- to 27-nm viruslike particles associated with two family outbreaks of gastroenteritis. J Infect Dis. 1977 Jan;135(1):20–27. [PubMed]
  • Wyatt RG, Dolin R, Blacklow NR, DuPont HL, Buscho RF, Thornhill TS, Kapikian AZ, Chanock RM. Comparison of three agents of acute infectious nonbacterial gastroenteritis by cross-challenge in volunteers. J Infect Dis. 1974 Jun;129(6):709–714. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)