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Table 1
Yeast strains and plasmids
GenotypeSource
Yeast strains
 YW5-1BMATa leu2-3, 112 trp1 ura3-52Tsukada and Ohsumi (1993) blue right-pointing triangle
 YIT701MATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2This study
 YIT702MATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2This study
 [pAPG7myc-314, pAPG12HA-316]
 YIT703MATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2This study
 [pAPG7myc-324, pAPG12HA-426]
 YIT704MATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2This study
 [pYO324, pAPG12HA-426]
 YIT7G333AMATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2This study
 [pAPG7G333Amyc-314, pAPG12HA-316]
 YIT7C507AMATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2This study
 [pAPG7C507 Amyc-314, pAPG12HA-316]
 YTS2MATa ura3-52 lys2-801 ade2-101 trp1 his3This study
 leu2 pho8::pho8Δ60 apg7::HIS3
 PJ69-4AMATa trp1-901 leu2-3, 112 ura3-52 his3-200James et al. (1996) blue right-pointing triangle
 gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3
 met2::GAL7-lacZ
Plasmids
 pRS314CEN6 TRP1Sikorski and Hieter (1989) blue right-pointing triangle
 pRS316CEN6 URA3Sikorski and Hieter (1989) blue right-pointing triangle
 pYO324TRP1Homma et al. (1998) blue right-pointing triangle
 pAPG7myc-314CEN6 TRP1 APG7-3 × cmyc epitopeThis study
 pAPG7myc-324TRP1 APG7-3 × cmyc epitopeThis study
 pAPG7G333Amyc-314CEN6 TRP1 APG7G333A-3 × cmyc epitopeThis study
 pAPG7C507Amyc-314CEN6 TRP1 APG7C507A-3 × cmyc epitopeThis study
 pAPG12HA-316CEN6 URA3 3 × HA epitope-APG12Mizushima et al. (1998) blue right-pointing triangle
 pAPG12HA-426URA3 3 × HA epitope-APG12Mizushima et al. (1998) blue right-pointing triangle
 pGAD-C1LEU2 PADH1::GAL4 ADJames et al. (1996) blue right-pointing triangle
 pGAD-APG12LEU2 PADH1::GAL4 AD-APG12This study
 pGBD-C1TRP1 PADH1::GAL4 BDJames et al. (1996) blue right-pointing triangle
 pGBD-APG7TRP1 PADH1::GAL4 BD-APG7-3 × cmyc epitopeThis study
 pGBD-APG7G333ATRP1 PADH1::GAL4 BD-APG7G333A-3 × cmyc epitopeThis study
 pGBD-APG7C507ATRP1 PADH1::GAL4 BD-APG7C507A-3 × cmyc epitopeThis study
Articles from Molecular Biology of the Cell are provided here courtesy of
American Society for Cell Biology