Apg7p interacts with Apg12p in vivo. pGBD-APG7 (TRP1) and pGAD-APG12 (LEU2) were transformed into PJ69-4A strain (gal4Δ gal80Δ trp1 leu2 GAL2-ADE2 GAL1-HIS3) to express GAL4BD-Apg7p and GAL4AD-Apg12p, respectively. pGAD-C1 and pGBD-C1 were used as controls. Cells were plated on SC-Trp-Leu plate (positive control) and SC-Ade-His-Trp-Leu plate, and incubated at 30°C for 3 d. PJ69-4A strain counterclockwise from the bottom carried pGBD-C1 and pGAD-C1 (GBD GAD), pGBD-C1 and pGAD-APG12 (GBD GAD-APG12), pGBD-APG7 and pGAD-C1 (GBD-APG7 GAD), and pGBD-APG7 and pGAD-APG12 (GBD-APG7 GAD-APG12). A strain expressing both GAL4BD-Apg7p and GAL4AD-Apg12p grew well on SC-Ade-Trp-Leu plate, indicating that Apg7p interacts with Apg12p.
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