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Antimicrob Agents Chemother. Aug 2008; 52(8): 2996–2997.
Published online May 19, 2008. doi:  10.1128/AAC.00325-08
PMCID: PMC2493102
Emergence of the Plasmid-Mediated Quinolone Resistance Gene qnrS1 in Escherichia coli Isolates in Greece[down-pointing small open triangle]
Olga Vasilaki
Department of Microbiology
AHEPA University Hospital
Thessaloniki, Greece
Eleni Ntokou and Alexandros Ikonomidis
Department of Medical Microbiology
University Hospital of Larissa
41110 Larissa, Greece
Danae Sofianou
Department of Microbiology
Hippokration Hospital of Thessaloniki
Thessaloniki, Greece
Filanthi Frantzidou and Styliani Alexiou-Daniel
Department of Microbiology
AHEPA University Hospital
Thessaloniki, Greece
Antonios N. Maniatis and Spyros Pournaras*
Department of Medical Microbiology
University Hospital of Larissa
41110 Larissa, Greece
*Phone: (30) 2410 682929, Fax: (30) 2410 681570, E-mail: pournaras/at/med.uth.gr
Three major groups of the plasmid-mediated quinolone resistance (Qnr) determinants have been identified so far in Enterobacteriaceae: the QnrA group, which includes 6 variants, the QnrB group, which includes 19 variants, and the QnrS group, which includes 3 variants (5). Although Qnr proteins produce only low-level resistance, they provide a favorable background for higher resistance to occur at quinolone concentrations that would be lethal in their absence, through secondary changes in DNA gyrase and topoisomerase IV, porin, or efflux systems (6). The purpose of our study was to investigate the presence and dissemination of the qnr genes among ciprofloxacin-resistant Escherichia coli isolates from different hospitals in Greece, a region with a relatively high frequency of quinolone resistance (1), where qnr genes had not been reported previously.
A total of 113 nonrepetitive ciprofloxacin-resistant E. coli clinical isolates were taken at random from the laboratory collections of four unrelated hospitals in northern and central Greece between 2006 and 2007 and analyzed for qnr genes. Ciprofloxacin MICs were estimated by the Etest (AB Biodisk, Solna, Sweden) and the agar dilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines (3) by using the breakpoints 1 and 4 μg/ml for susceptibility and resistance, respectively. E. coli ATCC 25922 was used as a control in all susceptibility assays; positive controls for the genes qnrA, qnrB, and qnrS were kindly provided by J. Sanchez-Cespedes. Susceptibilities to all antimicrobials tested were defined according to the CLSI interpretative criteria (3).
PCR was performed with primers amplifying all known qnr gene variants. The primer pair for the gene qnrA was 5′-AGAGGATTTCTCACGCCAGG-3′ and 5′-CCAGGCACAGATCTTGAC-3′ (yielding a 580-bp product), that for qnrB was 5′-GGGTATGGATATTATTGATAAAG-3′ and 5′-CTAATCCGGCAGCACTATTA-3′ (yielding a 264-bp product), and the primer pair for qnrS was 5′-GCAAGTTCATTGAACAGGGT-3′ and 5′-TCTAAACCGTCGAGTTCGGC-3′ (yielding a 428-bp product). The gene gyrA was amplified with primers 5′-TTAATGATTGCCGCCGTCGG-3′ and 5′-TACACCGGTCAACATTGAGG-3′ (yielding a 648-bp product) and parC was amplified with primers 5′-AAACCTGTTCAGCGCCGCATT-3′ and 5′-GTGGTGCCGTTAAGCAAA-3′ (yielding a 395-bp product) to evaluate possible coexisting chromosomal mutations. The corresponding specific PCR products were sequenced by LARK Technologies, Essex, United Kingdom.
For the qnr-positive isolates, synergy experiments were also performed using ciprofloxacin and the efflux pump inhibitor CCCP (carbonyl cyanide m-chlorophenylhydrazone) (8) to check the contribution of efflux pump overexpression to ciprofloxacin resistance. Pulsed-field gel electrophoresis (PFGE) analysis of XbaI-digested genomic DNA was performed, and the banding patterns of the strains were compared visually according to the criteria proposed by Tenover et al. (9). Filter mating experiments were performed with qnr-positive isolates by using E. coli 26R793 (lac negative and rifampin resistant) as the recipient. Transconjugants were selected on MacConkey agar plates containing 100 mg of rifampin/liter and 6 mg of nalidixic acid/liter, tested for qnr genes by PCR, and analyzed for plasmids by alkaline lysis.
Eleven of the 113 E. coli isolates (10%) derived from three independent hospitals in Thessaly (Larissa, central Greece) and Macedonia (Thessaloniki, northern Greece) and exhibiting nine unrelated PFGE strain patterns were qnr positive. The ciprofloxacin MICs for these isolates were 16 to 128 μg/ml; the characteristics of the isolates are presented in Table Table1.1. One Qnr-positive isolate (isolate 3) was an extended-spectrum β-lactamase producer carrying the gene blaCTX-M-15. No synergy between CCCP and ciprofloxacin in any isolate was observed. Sequencing of the PCR products showed that all 11 isolates carried the allele qnrS1, that none carried qnrA or qnrB, and that all had the mutations in the genes gyrA and parC that commonly confer ciprofloxacin resistance on E. coli isolates (2, 4) (Table (Table1).1). Mating experiments revealed that qnrS1 gene-carrying plasmids of various molecular sizes in 5 of the 11 isolates were transferable to the susceptible host. Ciprofloxacin MICs for the transconjugants were 0.25 to 0.5 μg/ml, while the MIC for the susceptible E. coli recipient was 0.032 μg/ml.
TABLE 1.
TABLE 1.
Characteristics of the 11 qnrS1-positive study isolates
The presence of the qnr genes in clinical isolates from Greece had not been reported previously. In this study, a considerably high proportion, 10%, of quinolone-resistant E. coli isolates were found to carry the gene qnrS1. The predominance in Greece of the qnr variant qnrS1, which was up to now detected mainly among salmonellae (4) and more rarely in E. coli (7) in Europe, indicates its possibly wide distribution. The carriage of qnrS1 in unrelated isolates of E. coli indicates either the natural existence of this gene in microbial populations or its wide horizontal spread through plasmids or integrons.
In conclusion, qnr genes seem to be common in ciprofloxacin-resistant clinical E. coli isolates and may contribute to the alarming rates of quinolone resistance in Greece.
Footnotes
[down-pointing small open triangle]Published ahead of print on 19 May 2008.
1. Chaniotaki, S., P. Giakouppi, L. S. Tzouvelekis, D. Panagiotakos, M. Kozanitou, G. Petrikkos, A. Avlami, the WHONET Study Group, and A. C. Vatopoulos. 2004. Quinolone resistance among Escherichia coli strains from community-acquired urinary tract infections in Greece. Clin. Microbiol. Infect. 10:75-78. [PubMed]
2. Chenia, H. Y., B. Pillay, and D. Pillay. 2006. Analysis of the mechanisms of fluoroquinolone resistance in urinary tract pathogens. J. Antimicrob. Chemother. 58:1274-1278. [PubMed]
3. Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing: 15th informational supplement. M100-S15. Clinical and Laboratory Standards Institute, Wayne, PA.
4. Hopkins, K. L., M. Day, and E. J. Threlfall. 2008. Plasmid-mediated quinolone resistance in Salmonella enterica, United Kingdom. Emerg. Infect. Dis. 14:340-342. [PMC free article] [PubMed]
5. Jacoby, G., V. Cattoir, D. Hooper, L. Martínez-Martínez, P. Nordmann, A. Pascual, L. Poirel, and M. Wang. 2008. qnr gene nomenclature. Antimicrob. Agents Chemother. 52:2297-2299. [PMC free article] [PubMed]
6. Jacoby, G. A. 2005. Mechanisms of resistance to quinolones. Clin. Infect. Dis. 41(Suppl. 2):S120-S126. [PubMed]
7. Poirel, L., C. Leviandier, and P. Nordmann. 2006. Prevalence and genetic analysis of plasmid-mediated quinolone resistance determinants QnrA and QnrS in Enterobacteriaceae isolates from a French University Hospital. Antimicrob. Agents Chemother. 50:3992-3997. [PMC free article] [PubMed]
8. Pournaras, S., M. Maniati, N. Spanakis, A. Ikonomidis, P. T. Tassios, A. Tsakris, N. J. Legakis, and A. N. Maniatis. 2005. Spread of efflux pump-overexpressing, non-metallo-β-lactamase-producing, meropenem-resistant but ceftazidime-susceptible Pseudomonas aeruginosa in a region with blaVIM-endemicity. J. Antimicrob. Chemother. 56:761-764. [PubMed]
9. Tenover, F. C., R. D. Arbeit, R. V. Goering, P. A. Mickelsen, B. E. Murray, D. H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33:2233-2239. [PMC free article] [PubMed]
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