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J Bacteriol. 1970 December; 104(3): 1266–1272.
PMCID: PMC248286

Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K-12


Crosses were carried out at 34 C and 42 C between eight pairs of isogenic strains of Escherichia coli K-12. The donor and recipient of each pair carried the same mutation for temperature-sensitive deoxyribonucleic acid (DNA) synthesis; they differed only in the presence of F-lac in the donor and a spectinomycin-resistance marker in the recipient. A different temperature-sensitive mutation was present in each of the eight pairs, the eight temperature-sensitive mutations being located in at least two different genes. In all eight pairs, the transfer of F-lac occurred at high and equal rates at 34 C and 42 C, although vegetative DNA replication at 42 C was approximately 10−4 of that at 34 C. The transfer of F-lac at 42 C was accompanied in seven of the eight crosses by an equivalent amount of DNA synthesis in excess of that observed in the unmated controls. The DNA synthesized during transfer at 42 C was characterized by equilibrium centrifugation in cesium chloride and by its sedimentation velocity in sucrose gradients. It was found to have a density and a molecular weight characteristic of F-lac DNA. A small proportion of the material labeled during transfer was recovered in the form of covalently closed DNA. It is concluded that vegetative replication of the chromosome and transfer replication of F are separate processes, the former requiring at least two gene products which are nonessential for the latter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Barbour SD. Effect of nalidixic acid on conjugational transfer and expression of episomal lac genes in Escherichia coli K12. J Mol Biol. 1967 Sep 14;28(2):373–376. [PubMed]
  • Bonhoeffer F. DNA transfer and DNA synthesis during bacterial conjugation. Z Vererbungsl. 1966;98(2):141–149. [PubMed]
  • Bouck N, Adelberg EA. Mechanism of action of nalidixic acid on conjugating bacteria. J Bacteriol. 1970 Jun;102(3):688–701. [PMC free article] [PubMed]
  • Boyle JV, Cook TM, Goss WA. Mechanism of action of nalidixic acid on Escherichia coli. Vi. Cell-free studies. J Bacteriol. 1969 Jan;97(1):230–236. [PMC free article] [PubMed]
  • Bresler SE, Lanzov VA, Lukjaniec-Blinkova AA. On the mechanism of conjugation in Escherichia coli K 12. Mol Gen Genet. 1968;102(4):269–274. [PubMed]
  • Buttin G, Wright M. Enzymatic DNA degradation in E. coli: its relationship to synthetic processes at the chromosome level. Cold Spring Harb Symp Quant Biol. 1968;33:259–269. [PubMed]
  • Curtiss R., 3rd Bacterial conjugation. Annu Rev Microbiol. 1969;23:69–136. [PubMed]
  • Fangman WL, Novick A. Characterization of two bacterial mutants with temperature-sensitive synthesis of DNA. Genetics. 1968 Sep;60(1):1–17. [PubMed]
  • Fisher KW, Fisher MB. Nalidixic acid inhibition of DNA transfer in Escherichia coli K12. Cold Spring Harb Symp Quant Biol. 1968;33:629–633. [PubMed]
  • Freifelder D. Studies on Escherichia coli sex factors. 3. Covalently closed F'Lac DNA molecules. J Mol Biol. 1968 May 28;34(1):31–38. [PubMed]
  • Freifelder DR, Freifelder D. Studies on Escherichia coli sex factors. II. Some physical properties of F'Lac and F DNA. J Mol Biol. 1968 Feb 28;32(1):25–35. [PubMed]
  • Hirota Y, Ryter A, Jacob F. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harb Symp Quant Biol. 1968;33:677–693. [PubMed]
  • JACOB F, ADELBERG EA. Transfert de caractéres génétiques par incorporation au facteur sexuel d'Escherichia coli. C R Hebd Seances Acad Sci. 1959 Jul 6;249(1):189–191. [PubMed]
  • MONOD J, COHEN-BAZIRE G, COHN M. Sur la biosynthèse de la beta-galactosidase (lactase) chez Escherichia coli; la spécificité de l'induction. Biochim Biophys Acta. 1951 Nov;7(4):585–599. [PubMed]
  • Moody EE, Lukin A. Chromosome transfer during bacterial mating. J Mol Biol. 1970 Mar 14;48(2):209–217. [PubMed]
  • Taylor AL, Trotter CD. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. [PMC free article] [PubMed]

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