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Published online 2008 June 11. doi: 10.1186/1755-8794-1-24

Figure 1

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SNPlex genotyping system assay protocol. On the first day, the OLA reaction, exonuclease purification, and PCR amplification are performed. On the second day, the amplicons are immobilized on streptavidin-coated microtiter plates. ZipChute probes are hybridized to complementary ZipCode sequences, and non-hybridized ZipChute probes are washed away. The bound ZipChute probes are eluted and analyzed by capillary electrophoresis using Applied Biosystems 3730. Reproduced from Journal of Biomolecular Techniques (Reference [18]) with permission from Andreas R Tobler at Applied Biosystems.

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