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J Virol. 1991 May; 65(5): 2231–2236.
PMCID: PMC240571

Human immunodeficiency virus type 1 infection of human placenta: potential route for fetal infection.


To determine the potential role of the placenta in transmission of human immunodeficiency virus (HIV) from mother to fetus, the ability of human placental tissue to support HIV type 1 (HIV-1) infection was examined. HIV-1-seronegative first-trimester placentas were maintained in culture and infected with HIV-1. Virus production, measured by HIV-1 antigen release into the supernatant, and HIV-1 DNA, identified by polymerase chain reaction, were detected for at least 12 days postinfection. Western immunoblot analysis showed Gag proteins, precursor p55, and cleavage products p24 and p17 in HIV-1-infected tissues. Double labeling of placental villi with antibodies to CD4 and placental trophoblast-specific alkaline phosphatase indicated that trophoblasts express CD4 antigen. Additionally, immunostaining of HIV-1-infected tissues with anti-p24 antibodies demonstrated HIV-1 protein expression in placental trophoblasts. Evaluation of human chorionic gonadotropin and progesterone production by the placental cultures indicated that there was a 90% decrease in human chorionic gonadotropin and a 70% decrease in progesterone production in HIV-1-infected cultures in comparison with controls. These data demonstrate that trophoblastic cells of human placenta tissue express CD4 and are susceptible to HIV-1 infection; also, placental endocrine function is decreased by HIV-1 infection. Thus, the placenta may serve as a reservoir of HIV-1 infection during pregnancy contributing to infection of the fetus, and decreased placental hormone production may result in impaired fetal development.

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  • Amirhessami-Aghili N, Lahijani R, Manalo P, St Jeor S, Tibbitts FD, Hall MR, Afsari A. Persistence of human cytomegalovirus DNA sequences without cell-virus homology in human placental explants in culture. Int J Fertil. 1989 Nov-Dec;34(6):411–419. [PubMed]
  • Blanche S, Rouzioux C, Moscato ML, Veber F, Mayaux MJ, Jacomet C, Tricoire J, Deville A, Vial M, Firtion G, et al. A prospective study of infants born to women seropositive for human immunodeficiency virus type 1. HIV Infection in Newborns French Collaborative Study Group. N Engl J Med. 1989 Jun 22;320(25):1643–1648. [PubMed]
  • Clouse KA, Powell D, Washington I, Poli G, Strebel K, Farrar W, Barstad P, Kovacs J, Fauci AS, Folks TM. Monokine regulation of human immunodeficiency virus-1 expression in a chronically infected human T cell clone. J Immunol. 1989 Jan 15;142(2):431–438. [PubMed]
  • Gloeb DJ, O'Sullivan MJ, Efantis J. Human immunodeficiency virus infection in women. I. The effects of human immunodeficiency virus on pregnancy. Am J Obstet Gynecol. 1988 Sep;159(3):756–761. [PubMed]
  • Hoff R, Berardi VP, Weiblen BJ, Mahoney-Trout L, Mitchell ML, Grady GF. Seroprevalence of human immunodeficiency virus among childbearing women. Estimation by testing samples of blood from newborns. N Engl J Med. 1988 Mar 3;318(9):525–530. [PubMed]
  • Jovaisas E, Koch MA, Schäfer A, Stauber M, Löwenthal D. LAV/HTLV-III in 20-week fetus. Lancet. 1985 Nov 16;2(8464):1129–1129. [PubMed]
  • Lewis SH, Reynolds-Kohler C, Fox HE, Nelson JA. HIV-1 in trophoblastic and villous Hofbauer cells, and haematological precursors in eight-week fetuses. Lancet. 1990 Mar 10;335(8689):565–568. [PubMed]
  • Luzi G, Ensoli B, Turbessi G, Scarpati B, Aiuti F. Transmission of HTLV-III infection by heterosexual contact. Lancet. 1985 Nov 2;2(8462):1018–1018. [PubMed]
  • Marion RW, Wiznia AA, Hutcheon RG, Rubinstein A. Fetal AIDS syndrome score. Correlation between severity of dysmorphism and age at diagnosis of immunodeficiency. Am J Dis Child. 1987 Apr;141(4):429–431. [PubMed]
  • Maury W, Potts BJ, Rabson AB. HIV-1 infection of first-trimester and term human placental tissue: a possible mode of maternal-fetal transmission. J Infect Dis. 1989 Oct;160(4):583–588. [PubMed]
  • Millán JL, Stigbrand T. "Sandwich" enzyme immunoassay for placental alkaline phosphatase. Clin Chem. 1981 Dec;27(12):2014–2018. [PubMed]
  • Minkoff H, Nanda D, Menez R, Fikrig S. Pregnancies resulting in infants with acquired immunodeficiency syndrome or AIDS-related complex: follow-up of mothers, children, and subsequently born siblings. Obstet Gynecol. 1987 Mar;69(3 Pt 1):288–291. [PubMed]
  • Mok JQ, Giaquinto C, De Rossi A, Grosch-Wörner I, Ades AE, Peckham CS. Infants born to mothers seropositive for human immunodeficiency virus. Preliminary findings from a multicentre European study. Lancet. 1987 May 23;1(8543):1164–1168. [PubMed]
  • Ou CY, Kwok S, Mitchell SW, Mack DH, Sninsky JJ, Krebs JW, Feorino P, Warfield D, Schochetman G. DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. Science. 1988 Jan 15;239(4837):295–297. [PubMed]
  • Pyun KH, Ochs HD, Dufford MT, Wedgwood RJ. Perinatal infection with human immunodeficiency virus. Specific antibody responses by the neonate. N Engl J Med. 1987 Sep 3;317(10):611–614. [PubMed]
  • Qazi QH, Sheikh TM, Fikrig S, Menikoff H. Lack of evidence for craniofacial dysmorphism in perinatal human immunodeficiency virus infection. J Pediatr. 1988 Jan;112(1):7–11. [PubMed]
  • Ryder RW, Nsa W, Hassig SE, Behets F, Rayfield M, Ekungola B, Nelson AM, Mulenda U, Francis H, Mwandagalirwa K, et al. Perinatal transmission of the human immunodeficiency virus type 1 to infants of seropositive women in Zaire. N Engl J Med. 1989 Jun 22;320(25):1637–1642. [PubMed]
  • Scott GB, Fischl MA, Klimas N, Fletcher MA, Dickinson GM, Levine RS, Parks WP. Mothers of infants with the acquired immunodeficiency syndrome. Evidence for both symptomatic and asymptomatic carriers. JAMA. 1985 Jan 18;253(3):363–366. [PubMed]
  • Spector DH, Wade E, Wright DA, Koval V, Clark C, Jaquish D, Spector SA. Human immunodeficiency virus pseudotypes with expanded cellular and species tropism. J Virol. 1990 May;64(5):2298–2308. [PMC free article] [PubMed]
  • Sprecher S, Soumenkoff G, Puissant F, Degueldre M. Vertical transmission of HIV in 15-week fetus. Lancet. 1986 Aug 2;2(8501):288–289. [PubMed]
  • Ultmann MH, Belman AL, Ruff HA, Novick BE, Cone-Wesson B, Cohen HJ, Rubinstein A. Developmental abnormalities in infants and children with acquired immune deficiency syndrome (AIDS) and AIDS-related complex. Dev Med Child Neurol. 1985 Oct;27(5):563–571. [PubMed]
  • Wright DA, Staprans SI, Spector DH. Four phosphoproteins with common amino termini are encoded by human cytomegalovirus AD169. J Virol. 1988 Jan;62(1):331–340. [PMC free article] [PubMed]

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