PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
 
Appl Environ Microbiol. 1985 August; 50(2): 431–437.
PMCID: PMC238638

Evidence for Separate Adhesion Mechanisms for Hydrophilic and Hydrophobic Surfaces in Vibrio proteolytica

Abstract

The proteolytic enzymes pronase, trypsin, and chymotrypsin and the surfactant Triton X-100 inhibited attachment of Vibrio proteolytica to the hydrophobic substratum polystyrene by >97%. These treatments had no effect on attachment to hydrophilic substrata such as glass or tissue culture dishes. Both pronase and Triton X-100 effected the removal of previously attached cells from polystyrene but not from hydrophilic surfaces. Removal of cells from polystyrene by pronase left material (which we have termed footprints) that stained with the protein-specific stain Hoechst 2495 but not with the DNA-specific stain Hoechst 33342. Pronase treatment also caused a significant decrease in cell surface hydrophobicity as determined by phase partitioning in hexane or petroleum ether. Collectively, these results imply the existence of separate mechanisms for the adhesion of V. proteolytica to hydrophilic and hydrophobic substrata and suggest a role for protein in the latter mechanism.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Dahlbäck B, Hermansson M, Kjelleberg S, Norkrans B. The hydrophobicity of bacteria - an important factor in their initial adhesion at the air-water interface. Arch Microbiol. 1981 Jan;128(3):267–270. [PubMed]
  • Dale JW. An inducible beta-lactamase in a strain of Escherichia coli. Antonie Van Leeuwenhoek. 1975;41(1):59–68. [PMC free article] [PubMed]
  • Lubic RW. The return of the American midwife. Aust Nurses J. 1977 Sep;7(3):28–32. [PMC free article] [PubMed]
  • Tulga M. Bronchographie: Das Verhalten der Blutgase und Säure-Basen-Status in der Lokalanästhesie oder Allgemeinnarkose. Prax Pneumol. 1975 Aug;29(8):448–452. [PMC free article] [PubMed]
  • Geesey GG, Richardson WT, Yeomans HG, Irvin RT, Costerton JW. Microscopic examination of natural sessile bacterial populations from an alpine stream. Can J Microbiol. 1977 Dec;23(12):1733–1736. [PubMed]
  • Handley PS, Carter PL, Fielding J. Streptococcus salivarius strains carry either fibrils or fimbriae on the cell surface. J Bacteriol. 1984 Jan;157(1):64–72. [PMC free article] [PubMed]
  • Jiwa SF, Månsson I. Hemagglutinating and hydrophobic surface properties of salmonellae producing enterotoxin neutralized by cholera anti-toxin. Vet Microbiol. 1983 Oct;8(5):443–458. [PubMed]
  • Jones HC, Roth IL, Sanders WM., 3rd Electron microscopic study of a slime layer. J Bacteriol. 1969 Jul;99(1):316–325. [PMC free article] [PubMed]
  • Ogawa M. The effects of prolactin, cortisol and calcium-free environment on water influx in isolated gills of Japanese eel, Anguilla japonica. Comp Biochem Physiol A Comp Physiol. 1975 Nov 1;52(3):539–543. [PMC free article] [PubMed]
  • Leith DK, Baseman JB. Purification of a Mycoplasma pneumoniae adhesin by monoclonal antibody affinity chromatography. J Bacteriol. 1984 Feb;157(2):678–680. [PMC free article] [PubMed]
  • Marshall KC, Cruickshank RH. Cell surface hydrophobicity and the orientation of certain bacteria at interfaces. Arch Mikrobiol. 1973 Apr 8;91(1):29–40. [PubMed]
  • Tawilab V. [Adult education]. Thai J Nurs. 1977 Jan;26(1):25–39. [PMC free article] [PubMed]
  • Ammon HP. Effect of tolbutamide on aminophylline-, 3,5-AMP-dibutyrate- or glucagon-induced insulin release from pancreatic islets after impairment of pyridine nucleotide metabolism caused by 6-aminonicotinamide (6-AN). Naunyn Schmiedebergs Arch Pharmacol. 1975;290(2-3):251–264. [PMC free article] [PubMed]
  • Paul JH. Effects of antimetabolites on the adhesion of an estuarine Vibrio sp. to polystyrene. Appl Environ Microbiol. 1984 Nov;48(5):924–929. [PMC free article] [PubMed]
  • Letter: Questions and comments on asthma therapy. Pediatrics. 1975 Jul;56(1):146–148. [PMC free article] [PubMed]
  • Wood C. The adult nurse practitioner. Alumni Mag. 1977 Feb;76(1):12–13. [PMC free article] [PubMed]
  • Reisfeld A, Rosenberg E, Gutnick D. Microbial degradation of crude oil: factors affecting the dispersion in sea water by mixed and pure cultures. Appl Microbiol. 1972 Sep;24(3):363–368. [PMC free article] [PubMed]
  • Rosenberg M, Judes H, Weiss E. Cell surface hydrophobicity of dental plaque microorganisms in situ. Infect Immun. 1983 Nov;42(2):831–834. [PMC free article] [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)