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J Virol. 1993 December; 67(12): 7680–7683.
PMCID: PMC238241

Identification of a lytic-phase origin of DNA replication in human herpesvirus 6B strain Z29.


DNA sequences which have structural features suggestive of their functioning as an origin of lytic-phase DNA replication were previously identified in both human herpesvirus 6B strain Z29 [HHV-6B (Z29)] and in HHV-6A (U1102). Plasmid constructs containing the putative HHV-6B (Z29) oriLyt element were replicated after transfection into permissive T cells, when trans-acting factors were provided by HHV-6B (R-1) infection. By using this assay, the HHV-6B (Z29) oriLyt was mapped to a minimal region of approximately 400 bp which lies upstream of the gene that is homologous to herpes simplex virus UL29, a region that carries an origin in other betaherpesviruses and in some alphaherpesviruses.

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Selected References

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  • Human herpesvirus-6 strain groups: a nomenclature. Arch Virol. 1993;129(1-4):363–366. [PubMed]
  • Ablashi DV, Balachandran N, Josephs SF, Hung CL, Krueger GR, Kramarsky B, Salahuddin SZ, Gallo RC. Genomic polymorphism, growth properties, and immunologic variations in human herpesvirus-6 isolates. Virology. 1991 Oct;184(2):545–552. [PubMed]
  • Anders DG, Kacica MA, Pari G, Punturieri SM. Boundaries and structure of human cytomegalovirus oriLyt, a complex origin for lytic-phase DNA replication. J Virol. 1992 Jun;66(6):3373–3384. [PMC free article] [PubMed]
  • Anders DG, Punturieri SM. Multicomponent origin of cytomegalovirus lytic-phase DNA replication. J Virol. 1991 Feb;65(2):931–937. [PMC free article] [PubMed]
  • Aubin JT, Agut H, Collandre H, Yamanishi K, Chandran B, Montagnier L, Huraux JM. Antigenic and genetic differentiation of the two putative types of human herpes virus 6. J Virol Methods. 1993 Feb;41(2):223–234. [PubMed]
  • Challberg MD. A method for identifying the viral genes required for herpesvirus DNA replication. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9094–9098. [PubMed]
  • Challberg MD, Kelly TJ. Animal virus DNA replication. Annu Rev Biochem. 1989;58:671–717. [PubMed]
  • Chee MS, Bankier AT, Beck S, Bohni R, Brown CM, Cerny R, Horsnell T, Hutchison CA, 3rd, Kouzarides T, Martignetti JA, et al. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr Top Microbiol Immunol. 1990;154:125–169. [PubMed]
  • Dewhurst S, Chandran B, McIntyre K, Schnabel K, Hall CB. Phenotypic and genetic polymorphisms among human herpesvirus-6 isolates from North American infants. Virology. 1992 Sep;190(1):490–493. [PubMed]
  • Dewhurst S, McIntyre K, Schnabel K, Hall CB. Human herpesvirus 6 (HHV-6) variant B accounts for the majority of symptomatic primary HHV-6 infections in a population of U.S. infants. J Clin Microbiol. 1993 Feb;31(2):416–418. [PMC free article] [PubMed]
  • Geller AI, Breakefield XO. A defective HSV-1 vector expresses Escherichia coli beta-galactosidase in cultured peripheral neurons. Science. 1988 Sep 23;241(4873):1667–1669. [PMC free article] [PubMed]
  • Gompels UA, Carrigan DR, Carss AL, Arno J. Two groups of human herpesvirus 6 identified by sequence analyses of laboratory strains and variants from Hodgkin's lymphoma and bone marrow transplant patients. J Gen Virol. 1993 Apr;74(Pt 4):613–622. [PubMed]
  • Hammerschmidt W, Sugden B. Identification and characterization of oriLyt, a lytic origin of DNA replication of Epstein-Barr virus. Cell. 1988 Nov 4;55(3):427–433. [PubMed]
  • Hamzeh FM, Lietman PS, Gibson W, Hayward GS. Identification of the lytic origin of DNA replication in human cytomegalovirus by a novel approach utilizing ganciclovir-induced chain termination. J Virol. 1990 Dec;64(12):6184–6195. [PMC free article] [PubMed]
  • Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. [PubMed]
  • Lawrence GL, Chee M, Craxton MA, Gompels UA, Honess RW, Barrell BG. Human herpesvirus 6 is closely related to human cytomegalovirus. J Virol. 1990 Jan;64(1):287–299. [PMC free article] [PubMed]
  • Masse MJ, Karlin S, Schachtel GA, Mocarski ES. Human cytomegalovirus origin of DNA replication (oriLyt) resides within a highly complex repetitive region. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5246–5250. [PubMed]
  • Miller CA, Kowalski D. cis-acting components in the replication origin from ribosomal DNA of Saccharomyces cerevisiae. Mol Cell Biol. 1993 Sep;13(9):5360–5369. [PMC free article] [PubMed]
  • Natale DA, Schubert AE, Kowalski D. DNA helical stability accounts for mutational defects in a yeast replication origin. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2654–2658. [PubMed]
  • Neipel F, Ellinger K, Fleckenstein B. The unique region of the human herpesvirus 6 genome is essentially collinear with the UL segment of human cytomegalovirus. J Gen Virol. 1991 Sep;72(Pt 9):2293–2297. [PubMed]
  • Pellett PE, Sánchez-Martínez D, Dominguez G, Black JB, Anton E, Greenamoyer C, Dambaugh TR. A strongly immunoreactive virion protein of human herpesvirus 6 variant B strain Z29: identification and characterization of the gene and mapping of a variant-specific monoclonal antibody reactive epitope. Virology. 1993 Aug;195(2):521–531. [PubMed]
  • Pruksananonda P, Hall CB, Insel RA, McIntyre K, Pellett PE, Long CE, Schnabel KC, Pincus PH, Stamey FR, Dambaugh TR, et al. Primary human herpesvirus 6 infection in young children. N Engl J Med. 1992 May 28;326(22):1445–1450. [PubMed]
  • Salahuddin SZ, Ablashi DV, Markham PD, Josephs SF, Sturzenegger S, Kaplan M, Halligan G, Biberfeld P, Wong-Staal F, Kramarsky B, et al. Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science. 1986 Oct 31;234(4776):596–601. [PubMed]
  • Schirmer EC, Wyatt LS, Yamanishi K, Rodriguez WJ, Frenkel N. Differentiation between two distinct classes of viruses now classified as human herpesvirus 6. Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5922–5926. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Spaete RR, Frenkel N. The herpes simplex virus amplicon: a new eucaryotic defective-virus cloning-amplifying vector. Cell. 1982 Aug;30(1):295–304. [PubMed]
  • Thomson BJ, Efstathiou S, Honess RW. Acquisition of the human adeno-associated virus type-2 rep gene by human herpesvirus type-6. Nature. 1991 May 2;351(6321):78–80. [PubMed]
  • Vlazny DA, Frenkel N. Replication of herpes simplex virus DNA: localization of replication recognition signals within defective virus genomes. Proc Natl Acad Sci U S A. 1981 Feb;78(2):742–746. [PubMed]
  • Weller SK, Spadaro A, Schaffer JE, Murray AW, Maxam AM, Schaffer PA. Cloning, sequencing, and functional analysis of oriL, a herpes simplex virus type 1 origin of DNA synthesis. Mol Cell Biol. 1985 May;5(5):930–942. [PMC free article] [PubMed]
  • Wu CA, Nelson NJ, McGeoch DJ, Challberg MD. Identification of herpes simplex virus type 1 genes required for origin-dependent DNA synthesis. J Virol. 1988 Feb;62(2):435–443. [PMC free article] [PubMed]
  • Yamanishi K, Okuno T, Shiraki K, Takahashi M, Kondo T, Asano Y, Kurata T. Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet. 1988 May 14;1(8594):1065–1067. [PubMed]

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