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Cyclin-dependent kinase inhibitors, including p21Cip1, are implicated in cell turnover and are active players in cardiovascular wound repair. Here, we show that p21Cip1 orchestrates the complex interactions between local vascular and circulating immune cells during vascular wound repair. In response to femoral artery mechanical injury, mice with homozygous deletion of p21Cip1 displayed accelerated proliferation of VSMCs and increased immune cell infiltration. BM transplantation experiments indicated that local p21Cip1 plays a pivotal role in restraining excessive proliferation during vascular wound repair. Increased local vascular stromal cell–derived factor-1 (SDF-1) levels were observed after femoral artery injury in p21+/+ and p21–/– mice, although this was significantly greater in p21–/– animals. In addition, disruption of SDF-1/CXCR4 signaling inhibited the proliferative response during vascular remodeling in both p21+/+ and p21–/– mice. We provide evidence that the JAK/STAT signaling pathway is an important regulator of vascular SDF-1 levels and that p21Cip1 inhibits STAT3 binding to the STAT-binding site within the murine SDF-1 promoter. Collectively, these results suggest that p21Cip1 activity is essential for the regulation of cell proliferation and inflammation after arterial injury in local vascular cells and that the SDF-1/CXCR4 signaling system is a key mediator of vascular proliferation in response to injury.
Vascular wound repair is controlled by the interaction of local vascular cells (endothelial and smooth muscle) and infiltrating inflammatory cells (macrophages, neutrophils, and lymphocytes). Particularly during arterial wound healing, a balanced control of vascular cell growth and death critically regulates the determination of both the composition of the healed arterial wall and luminal patency. Normally, during vascular homeostasis there is a low turnover rate of endothelial and smooth muscle cells. However, following arterial injury there is disruption of vessel architecture, triggering the early release of growth factors and inflammatory modulators that initiate a further cascade of downstream events (1, 2). Circulating inflammatory and progenitor cells are recruited to the site of injury and infiltrate the damaged vessel via the vessel lumen or the vasa vasorum, while previously quiescent local vascular cells also enter the cell cycle and proliferate (3). Although many cells participate in this early response to vascular injury, monocytes/macrophages have been noted as being particularly abundant (4). The recruitment of monocytes/macrophages is mediated by the chemokine stromal cell–derived factor-1 (SDF-1), which is upregulated at the site of tissue injury (5). SDF-1 is selectively bound by the chemokine receptor CXCR4, which is expressed on macrophages (6) and a wide range of other cells, including VSMCs (7, 8). CXCR4 signaling is mediated by G protein–dependent PI3K signal transduction pathways and the G protein–independent JAK/STAT pathway (9, 10).
The Cip/Kip proteins (p21Cip1, p27Kip1, and p57Kip2) bind to and alter the activities of cyclin D–, cyclin E–, and cyclin A–dependent kinases in quiescent cells (11, 12). The cyclin-dependent kinase inhibitor (CKI) p21Cip1 was initially identified as a potent inhibitor of cell cycle progression (13–16). Subsequent studies further identified that p21Cip1 has an important role in controlling cytostasis and cell death (17). Interestingly, it has also been shown that at low levels, p21Cip1 may have growth-permissive effects on cells by promoting the assembly of the CDK/cyclin D complex (18, 19). p21Cip1 transcription is activated by p53, and p21Cip1 is part of a negative feedback mechanism that controls p53 activity during apoptosis (20). p21Cip1 has been shown to be an important mediator of inflammation, VSMC proliferation (21, 22), and vascular proliferative disease (23–27). Of particular relevance, p21 knockout mice have been shown to exhibit enhanced neointimal formation following arterial injury (28). Similarly, in models of vascular wound repair, p27Kip1 has been shown to be an important modulator of vascular remodeling during the wound healing process (4, 29). Also, both p21Cip1 and p27Kip1 are known to be involved with the antiproliferative effects of sirolimus, a drug that is loaded onto “drug-coated” endovascular stents used in the treatment of ischemic heart disease (30–33).
Recently, p21Cip1 was identified as not just a CKI, but also an important transcriptional regulator (34, 35). Thus, p21Cip1 has been shown to regulate the activity of NF-κB, c-Myc, C/EBP, E2F, and STAT3 (36–39). The potential contribution of this aspect of p21Cip1 activity during vascular wound repair is unknown. Interestingly, the apparent paradox that p21Cip1 is not expressed in normal quiescent vessels but is upregulated in the proliferative phase of vascular remodeling may indicate an additional role besides the inhibition of cell cycle progression (40).
The present study was undertaken to delineate the functions of p21Cip1 in vascular and circulating inflammatory cells during arterial wound repair. Our results indicate that SDF-1/CXCR4 signaling mediates the local inflammatory and cellular proliferative response after arterial injury in WT mice and that blockade of this pathway leads to decreased neointimal formation. We provide evidence that p21Cip1 has a particularly important role in restraining local levels of VSMC-derived SDF-1 and that after vascular injury, p21–/– mice show an increase in both SDF-1 expression and neointimal formation as compared with WT animals. In addition, we show that vascular SDF-1 is transcriptionally regulated through the JAK/STAT pathway as part of a positive feedback loop and that p21Cip1 represses this process by binding to STAT3 and decreasing the STAT3-dependent transcriptional activation of the SDF-1.
Defining the roles of p21Cip1 and SDF-1 in the vascular repair process provides essential information regarding their function in the development of cardiovascular disease and may assist in the development of novel strategies to ameliorate this condition.
p21Cip1 is known to modulate apoptosis in several cell types, including endothelial cells, VSMCs, cardiomyocytes, and tumor cells (41–44). Therefore, we hypothesized that p21Cip1 may modulate cellular proliferation and susceptibility to apoptosis during vascular wound repair, and we initially examined these cellular functions of p21Cip1 in VSMCs. Low-passage p21+/+ and p21–/– VSMCs were seeded in equal number, and cellular growth was recorded. After 96 hours, there were 2.5-fold more p21–/– than p21+/+ VSMCs (Figure (Figure1A),1A), indicating that p21–/– VSMCs had a higher growth rate than p21+/+ VSMCs. Next, cellular stress and apoptosis were induced by adding increasing concentrations of either H2O2 or FasL to the culture medium. In the absence of H2O2 or FasL, the percentage of apoptotic p21–/– and p21+/+ VSMCs was similar (Figure (Figure1B1B and Supplemental Figure 1; supplemental material available online with this article; doi: 10.1172/JCI31244DS1). However, upon induction of cellular stress with increasing doses of H2O2 or FasL, the percentage of apoptotic cells increased significantly in the p21–/– group compared with the p21+/+ group (Figure (Figure1B1B and Supplemental Figure 1). These results confirm that p21Cip1 supports cell viability during cellular stress and also inhibits VSMC proliferation.
We used an established model of femoral artery wire injury to study the role of p21Cip1 in the vascular repair process (45). First, we explored p21Cip1 mRNA and protein levels in p21+/+ mice during arterial wound repair. Quantitative PCR and western blot analyses revealed that both p21Cip1 protein and mRNA levels were low in uninjured femoral arteries (Figure (Figure2A).2A). However, 3 days after vascular injury, p21Cip1 protein and mRNA levels were markedly upregulated, the latter more than 90-fold (Figure (Figure2A).2A). p21Cip1 mRNA subsequently decreased at later stages of the arterial repair process (Figure (Figure2A).2A). Since p21Cip1 transcription is activated by p53, we explored p53 expression in the context of the vascular injury. We observed that p53 mRNA levels followed the same pattern as p21Cip1 mRNA. Thus, p53 mRNA was near the limits of detection in uninjured vessels, but was elevated 20-fold 3 days after injury and subsequently decreased by 7 days after injury (Figure (Figure2B).2B). Confocal microscopic imaging confirmed the induction of nuclear p21Cip1 and p53 expression in VSMCs within the medial arterial layer (Figure (Figure2,2, C and D). These results suggest that p21Cip1 may be at least partially regulated by p53 in the early phase of the vascular remodeling.
Next, we explored whether p21Cip1 modulates arterial wound repair. We performed vascular injury in p21–/– and p21+/+ mice and examined cell proliferation and lesion formation. Cell proliferation was determined by in vivo BrdU pulsing and was significantly increased within the neointima of p21–/– arteries compared with p21+/+ arteries at 1 and 2 weeks after injury (Figure (Figure3,3, A and C). Correspondingly, there was significantly greater neointimal formation in p21–/– mice when compared with p21+/+ mice at both the 1- and 2-week time points (Figure (Figure3,3, B and C). These observations confirm the role of p21Cip1 in vascular wound repair and demonstrate that this process is greatly compromised in p21–/– mice compared with p21+/+ mice.
We noted a significant accumulation of mononuclear cells within the adventitia, media, and neointima after arterial injury and undertook further characterization of these cells. Histological sections were stained with antibodies specific for macrophages, neutrophils, and T lymphocytes at 1 and 2 weeks after injury (Figure (Figure4).4). Compared with p21+/+ mice, in p21–/– mice 1 week after injury, the number of macrophages in the adventitia, media, and neointima was 3.8-fold greater. However, there was no difference in macrophage counts 2 weeks after injury (Figure (Figure4A).4A). There were twice as many neutrophils in the lesions of p21–/– mice compared with p21+/+ mice 1 week after arterial injury, but interestingly, this trend was reversed in the later phase of arterial wound repair (Figure (Figure4B).4B). In contrast, the number of T lymphocytes was increased in the lesions of p21–/– mice compared with those of p21+/+ mice at both time points (Figure (Figure4C).4C). In summary, at the 1-week time point after arterial injury, all 3 inflammatory cells (macrophages, neutrophils, and T lymphocytes) were more abundant in the lesions of p21–/– mice than in those of p21+/+ mice. However, p21Cip1 deletion generally failed to sustain this enhanced inflammatory response in the later phase of arterial wound repair. Interestingly, we found no difference between p21–/– and p21+/+ mice in the number of circulating inflammatory cells or the percentage of monocyte subtypes, at baseline and after wire injury (Supplemental Table 1 and Supplemental Figure 2, A and B). These data show that, particularly in the early phase of arterial wound repair, p21Cip1 acts to decrease the migration and trafficking of inflammatory cells into areas of vascular damage.
To delineate the specific function of p21Cip1 in local vascular cells versus that in circulating immune cells, we performed BM transplantation from p21–/– or p21+/+ donors into p21–/– or p21+/+ recipient mice. Successful engraftment was confirmed by the detection of the SRY gene in the blood of female recipients that received male BM by quantitative PCR (data not shown). Mice underwent vascular injury, and neointimal formation was determined 2 weeks later. When p21–/– mice received BM from p21+/+ mice, neointimal formation was decreased compared to p21–/– mice that received p21–/– BM (Figure (Figure5A).5A). However, when p21+/+ mice received BM from p21–/– mice, arterial wound repair was not altered, and neointimal formation was identical to that seen in p21+/+ mice that received p21+/+ BM (Figure (Figure5A).5A). These experiments indicate that local vascular p21Cip1 is sufficient to restrain excessive neointimal formation. In addition, p21Cip1 deletion in local vascular cells caused increased infiltration of monocytes/macrophages (Figure (Figure5B). 5B).
We then determined the influence of p21Cip1 expression on apoptosis during arterial wound repair. Using a TUNEL assay, we found that at 1 week after arterial injury the percentage of apoptotic cells was significantly increased in p21–/– mice compared with p21+/+ mice, although this difference was not sustained at 2 weeks after injury (Supplemental Figure 3A). In BM transplantation experiments 1 week after injury, transplantation of p21–/– BM into p21+/+ recipients did not significantly increase the percentage of vascular apoptotic cells compared with p21+/+ mice receiving p21+/+ BM. Also, transplantation of p21+/+ BM into p21–/– recipient mice did not significantly decrease this process compared with transplantation of p21–/– BM into p21–/– recipient mice (Supplemental Figure 3B). Thus, p21Cip1 within local vascular cells appears to influence apoptosis after vascular injury, supporting the importance of local p21Cip1 function in vascular wound repair.
As a whole, these observations led us to question what distinguishes local p21+/+ from p21–/– vascular cells, and in particular, what factors might mediate the interactions of these resident vascular cells with circulating immune and inflammatory cells.
As SDF-1 is known to play an important role in recruiting and detaining inflammatory cells at the site of vascular injury (5, 46), we speculated that local vascular SDF-1 may be differentially regulated in p21–/– versus p21+/+ mice. Initial in vitro experiments confirmed increased protein levels of SDF-1 in p21–/– versus p21+/+ VSMCs. After the induction of oxidative stress with H2O2, SDF-1 levels were significantly reduced in both p21–/– and p21+/+ VSMCs (Supplemental Figure 4). Moving to our in vivo models, we first established that serum SDF-1 levels did not differ, before or 3 and 7 days after vascular injury, between p21+/+ and p21–/– mice (data not shown). We then proceeded to investigate local vascular SDF-1 levels at these time points. In the absence of arterial injury, there was no difference in SDF-1 tissue levels between p21+/+ and p21–/– mice. After injury, both p21+/+ and p21–/– mice exhibited increased local SDF-1 levels compared with uninjured controls. However, in p21–/– mice this increase both was of a greater magnitude and also, unlike p21+/+ mice, was sustained until at least 7 days after injury (Figure (Figure6,6, A and B). In order to further explore this observation, local vascular SDF-1 levels were also measured in p21+/+ and p21–/– mice that had undergone BM transplantation. Local SDF-1 was upregulated in p21–/– mice that received p21+/+ BM compared with p21+/+ mice that received p21+/+ BM. In addition, p21+/+ mice receiving p21–/– BM had similar levels of SDF-1 compared with p21+/+ mice that received p21+/+ BM (Figure (Figure6C).6C). These results indicate that p21Cip1 expression (or lack thereof) by BM-derived cells has minimal influence on vascular SDF-1 levels after injury, and that it is local p21Cip1 expression that modulates vascular SDF-1 levels.
Our results presented above suggest an important role for local vascular p21Cip1 in the modulation of vascular SDF-1 levels. However, while SDF-1 has been implicated in the regulation of cellular migration in this (femoral artery wire injury) and other situations (47), the precise anatomical localization of SDF-1 during wound repair, at a cellular level, is not well described. We explored SDF-1 expression by immunohistochemistry in uninjured vessels and during the early phase of vascular wound repair. As indicated by double staining for VSMC-specific α-actin and SDF-1, in uninjured p21+/+ and p21–/– murine femoral arteries, SDF-1 was predominantly expressed by VSMCs (Figure (Figure6A).6A). We did not observe significant staining in the endothelial cell layer (double staining for CD31 and SDF-1) or in the adventitia. This pattern of vascular SDF-1 expression was not altered by arterial injury. In addition, we did not detect obvious SDF-1 staining in either CD45-positive inflammatory cells, or F4/80-positive macrophages (Figure (Figure6A6A and data not shown).
SDF-1 signaling is mediated via the CXCR4 receptor, which is commonly expressed on immune and vascular cells. We hypothesized that apart from playing an important role in WT animals, inhibition of SDF-1 signaling would reverse the hyperproliferative phenotype in p21–/– mice. We utilized 2 independent approaches to inhibit SDF-1/CXCR4 function. First, we administered AMD3100, a specific CXCR4 inhibitor, to p21+/+ and p21–/– mice via osmotic minipumps and examined the inflammatory response and neointimal formation 1 and 2 weeks after vascular injury. Next, we inhibited the SDF-1/CXCR4 signaling pathway by treatment with a neutralizing monoclonal antibody against CXCR4 (48). As shown in Figure Figure7,7, A and B, the inhibition of SDF-1/CXCR4 signaling by either approach significantly reduced neointimal formation after vascular injury in p21–/– mice. CXCR4 blocking antibody also significantly inhibited neointimal formation in p21+/+ mice (Figure (Figure7B).7B). However, possibly due to the additional early BM cell–mobilizing effect of AMD3100 and consistent with other reports (49), we observed that in p21+/+ mice this reduction was only significant at the 2-week time point (Figure (Figure7A).7A). Cellular proliferation, as assessed by BrdU incorporation, was significantly decreased by both CXCR4 blocking antibody and AMD3100 in p21–/– and p21+/+ mice at 1 and 2 weeks after vascular injury (Figure (Figure7C7C and Supplemental Figure 5A). We further identified that either approach to inhibiting SDF-1/CXCR4 signaling was associated with reduced macrophage infiltration at 1 and 2 weeks after vascular injury in both p21–/– and p21+/+ mice (Figure (Figure7D7D and Supplemental Figure 5B). In contrast, neither CXCR4 blocking antibody nor AMD3100 administration altered cellular apoptosis in p21–/– or p21+/+ mice after vascular injury (Figure (Figure7E7E and Supplemental Figure 5C). Interestingly, 1 week after vascular injury, p21–/– mice displayed a marginal increase in local vascular SDF-1 levels after treatment with AMD3100. However, this treatment did not influence vascular SDF-1 levels in p21+/+ mice (Supplemental Figure 5D). Overall, these data confirmed the important influence of p21Cip1 on the SDF-1/CXCR4 signaling pathway during vascular remodeling.
We then investigated the mechanism whereby p21Cip1 modulates SDF-1 levels. Recent publications suggest that p53 represses SDF-1 and its receptor CXCR4 as part of a negative regulatory loop (50, 51). We hypothesized that p21Cip1 might also play an important role in this pathway and connect the cellular stress repair program to the inflammatory response. We screened the murine SDF-1 promoter region for possible transcription factors associated with p21Cip1 and were able to localize a putative STAT binding site (–516/–527 in the murine SDF-1 promoter region). This was consistent with previous reports that JAK/STAT is an important mediator of SDF-1/CXCR4 signaling (9) and that STAT3 is a major transcriptional activator of SDF-1 and may also act upstream of SDF-1 by modulating HIF-1α activity (52). We initially explored the potential p21Cip1/STAT3/SDF-1 interactions in primary murine VSMCs. A luciferase construct containing the –2,200-bp SDF-1 promoter sequence was transfected into p21+/+ and p21–/– VSMCs. As shown in Figure Figure8A,8A, this transfection resulted in a significant increase in SDF-1 promoter activity in p21–/– compared with p21+/+ VSMCs.
Next, we explored whether activation or inhibition of the STAT3 binding site within the SDF-1 promoter serves to modulate the expression of SDF-1 in VSMCs. After serum starvation of VSMCs for 24 hours, p21–/– VSMCs exhibited a 3-fold increase in SDF-1 mRNA expression compared with p21+/+ VSMCs (Figure (Figure8B).8B). Subsequently, the addition of leukemia inhibitory factor (LIF), which is known to activate STAT3 (53), to the culture medium caused a further increase in SDF-1 mRNA expression (Figure (Figure8B).8B). This activation was blocked by the cell membrane–permeable STAT3 inhibitor PpYLKTK-mts (Figure (Figure8B).8B). We then mutated the STAT3 binding site of our luciferase construct of the SDF-1 promoter sequence by a 4-bp substitution-mutation of the STAT3 binding site and transfected it into p21+/+ and p21–/– VSMCs. As shown in Figure Figure8C,8C, this caused a significant decrease in luciferase activity in both p21+/+ and p21–/– cells compared with transfection with the nonmutated SDF-1 promoter construct. Further experiments also demonstrated that increased STAT3 activation through Tyr705 phosphorylation was present in p21–/– VSMCs compared with p21+/+ VSMCs, and reciprocal coimmunoprecipitation experiments in VSMCs revealed an interaction between p21Cip1 and STAT3 (Figure (Figure8D). 8D).
Finally, we investigated whether STAT3 occupancy of the SDF-1 promoter is increased in p21–/– compared with p21+/+ VSMCs. This was achieved by analyzing the chromatin of human VSMCs by ChIP using primers that flank the –576/–372 region of the SDF-1 promoter that we previously identified as being likely to contain a STAT3 binding site (see above). PCR analysis of the chromatin immunoprecipitates, isolated with anti-STAT3 antibodies, showed increased STAT3 occupancy of the SDF-1 promoter in p21–/– compared with p21+/+ VSMCs (Figure (Figure8E).8E). These findings indicate that, at least in vitro, p21Cip1 is likely to influence SDF-1 levels in VSMCs by modulating STAT3 transcriptional activity. To further explore this possibility in vivo, we performed a ChIP assay on femoral arteries from p21–/– and p21+/+ mice at both 3 and 7 days after vascular injury. We observed an enhanced and prolonged STAT3 occupancy of the SDF-1 promoter in vessels from p21–/– mice compared with vessels from p21+/+ mice (Figure (Figure9,9, A and B). In summary, these data suggest that p21Cip1 modulates the inflammatory response and SDF-1 through the JAK/STAT signal transduction pathway in vitro and in vivo during vascular wound repair.
Arterial wound repair is a complex process involving predominantly local vascular cells and circulating inflammatory cells. Despite the fact that this process is directly implicated in the etiology of clinical ischemic heart disease, the precise functioning of the relevant inter- and intracellular signaling pathways is not entirely understood (54, 55). Here, we provide direct evidence that p21Cip1 plays a central role in vascular repair after injury. In brief, the major new findings to arise from this work are as follows: (a) Deletion of p21Cip1 enhances the inflammatory response during vascular wound repair. (b) Within medial VSMCs, p21Cip1 restrains SDF-1 levels in the early phase of vascular remodeling. (c) Inhibition of SDF-1/CXCR4 signaling by the CXCR4-specific receptor antagonist AMD3100, or a CXCR4-blocking monoclonal antibody, acts to limit inflammation and neointimal formation in p21+/+ and p21–/– mice during vascular wound repair. (d) VSMC-derived SDF-1 is transcriptionally regulated by the JAK/STAT signaling pathway. (e) p21Cip1 modulates the occupancy of STAT3 on the SDF-1 promoter in VSMCs both in vitro and in vivo during vascular remodeling.
p21Cip1 is acknowledged as being a key inhibitory regulator of cellular proliferation and a mediator of p53-dependent cellular apoptosis (35) and is considered to be a fundamental player in vascular wound repair (21, 23, 24, 26). To date, supporting evidence has included the observation that overexpression of recombinant p21Cip1 decreases VSMC proliferation and attenuates the progression of vascular proliferative diseases (21, 23, 24, 26) and that, conversely, reduced p21Cip1 expression leads to increased neointimal formation following arterial injury (28). This has led to the evaluation of novel treatment strategies for these diseases that are at least partially mediated through p21Cip1 upregulation (25, 56, 57). Our results further extend this work and serve to reinforce the important role of p21Cip1 in modulating vascular wound repair. We now additionally show that the hyperproliferative phenotype seen in p21Cip1–/– mice is associated with an increased number of inflammatory macrophages in the early phase of vascular wound repair. While other researchers have investigated this phenomenon in different settings, our findings are broadly consistent with the fact that p21Cip1 plays an important role in regulating cell proliferation and monocyte/macrophage differentiation (21, 22). However, BM transplantation experiments demonstrated that the enhanced neointimal formation that was observed in p21–/– mice could not be recapitulated by transplanting p21–/– BM into p21+/+ mice. Collectively, our experiments suggest that the hyperproliferative vascular phenotype of p21–/– mice is largely independent of BM-derived cells, but rather arises from the effects of p21Cip1 on the local vascular milieu. Interestingly, this latter finding is in direct opposition to the situation with p27Kip1, which primarily exerts its effects on arterial wound repair through infiltrating BM-derived immune cells (4).
Vascular wound repair is orchestrated by various cytokines and inflammatory mediators. While local SDF-1 levels were increased after vascular injury in p21+/+ mice, we observed that p21–/– mice exhibit a significantly greater increase in local SDF-1 levels after arterial injury. This finding was not recapitulated by transplanting p21–/– BM into p21+/+ mice, again suggesting that the effect of p21Cip1 is at the local vascular level. These results also implicated p21Cip1 in the regulation of SDF-1, and we therefore explored these potential interrelationships within the context of vascular wound repair. This was achieved by abrogating SDF-1/CXCR4 signaling using either the selective CXCR4 receptor antagonist AMD3100 (58) or a monoclonal antibody directed against CXCR4. Strengthening our hypothesis that p21Cip1 is involved with SDF-1 regulation, we found that either approach led to decreased neointimal formation in both p21+/+ and p21–/– mice. However, perhaps due to their higher degree of neointimal formation, we found that p21–/– mice exhibited a greater reduction in neointimal formation after SDF-1/CXCR4 blockade than did p21+/+ animals.
Numerous publications have described a central role for the SDF-1/CXCR4 axis in new vessel formation, vascular wound repair, and VSMC proliferation (5, 48, 59–63). Zernecke et al. (63) found that the transplantation of CXCR4–/– fetal liver cells into ApoE–/– mice resulted in a greater than 50% reduction in neointimal area after vascular wire injury, suggesting a regulatory role for inflammatory and/or immune cells in wound repair and VSMC proliferation. Similarly, Grunewald et al. (5) reported that tissue-specific hepatic and cardiac VEGF overexpression stimulated SDF-1 expression in perivascular “fibroblastic or smooth muscle” cells, which was sufficient to recruit and retain circulating CD45+CD11b+CXCR4+ myeloid cells. These investigators also demonstrated that the induction of perivascular SDF-1 expression is an integral aspect of angiogenesis and that the administration of a CXCR4 antagonist significantly inhibits this process (5). Recently, SDF-1 was also shown to be an important downstream mediator of HIF-1α, both of which were found to be upregulated during the hyperproliferative response to vascular wire injury in ApoE–/– mice (64). Our results corroborate these publications, and we similarly found that SDF-1/CXCR4 signaling is of importance in both p21–/– and p21+/+ mice. However, we further expand these intricate regulatory networks and suggest that p21Cip1 is an important mediator of SDF-1 transcription.
p21Cip1 is known to be a direct modulator of several transcription factors including STAT3 (34, 35, 37). We investigated this relationship in the context of SDF-1 transcription and were able to identify a putative STAT3-binding site within the murine SDF-1 promoter region. We also demonstrated that in VSMCs, activation of STAT3 signaling increases SDF-1 expression and, conversely, that inhibition of STAT3 signaling decreases SDF-1. In addition, we performed ChIP experiments on VSMCs and found that STAT3 occupancy could be detected on the SDF-1 promoter. Although SDF-1 was described as signaling through the JAK/STAT pathway almost a decade ago (9), we are unaware of any previous reports describing this reciprocal signaling arrangement, whereby the JAK/STAT pathway regulates SDF-1 transcription.
Integrating our findings, we suggest that in VSMCs and during vascular wound repair, p21Cip1 is able to modulate vascular SDF-1 levels by inhibiting the binding of STAT3 to the SDF-1 promoter. Adding complexity, it has also recently been shown that p21Cip1 is a direct STAT3 target, and furthermore, that STAT3 functions as a transcriptional repressor of p53, an important transcriptional activator of p21Cip1 (65, 66). However, it is also known that STAT3 and HIF-1α form a complex that regulates VEGF transcription (52), thus the possibility exists that HIF-1α also participates in the regulation of SDF-1 via this higher order transcriptional complex. Therefore, considering all available data and reports, we consider the following possible in the context of vascular wound repair and remodeling: (a) A positive feedback loop for SDF-1 operates via JAK/STAT signaling in the early phase of this process. (b) A negative feedback loop controls STAT3 transcriptional activity through p21Cip1, connecting the p53-mediated cellular stress response to the inflammatory response and restraining the latter in a p21Cip1-dependent manner. (c) Medial VSMCs modulate the immune response to vascular injury by the synthesis and secretion of SDF-1.
Importantly, numerous aspects of this study are of clinical relevance. However, none is perhaps more obvious than the potential application of our findings to vascular stent technologies. Thus, we speculate that several of the important regulators of the vascular remodeling process that were identified in this study (or their inhibitors) may have potential clinical utility if appropriately loaded onto “drug-eluting” stents. Conceivably, this could be either as adjuncts to, or replacements for, either sirolimus or paxlitaxel, the agents commonly used for this purpose at the current time.
In conclusion, our findings indicate that p21Cip1 plays an important role in the modulation of vascular remodeling after arterial injury. In local vascular cells, p21Cip1 restrains proliferation and the inflammatory response during vascular wound repair. Under the influence of p21Cip1, VSMC-derived SDF-1 plays a pivotal role in recruiting inflammatory cells to the injured area, and we propose that p21Cip1 regulates SDF-1 via the JAK/STAT signaling pathway. Importantly, this provides a possible link between the p53/p21Cip1-mediated cellular stress signaling program and the inflammatory response during vascular remodeling. Collectively, these findings suggest a central role for SDF-1 in vascular injury repair. We are cautiously optimistic that the targeted manipulation of key biologic regulators of the vascular repair process, in particular p21Cip1 and the SDF-1/CXCR4 axis, may be useful clinical adjuncts in the treatment or primary prevention of atherosclerotic vascular disease.
Heterozygous 129/B6 p21+/– mice were obtained from Tyler Jacks (David H. Koch Institute for Integrative Cancer Research at MIT, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA). All animal experiments were approved by the National Heart, Lung, and Blood Institute Animal Care and Use Committee. We back bred the mice for 12 generations against a C57BL/6 background and studied male and female mice at 10 weeks of age. p21+/+ littermates were used as controls. Genotyping was performed by PCR amplification of mouse tail DNA using allele-specific probes. Each experimental group contained a minimum of 5 mice.
VSMCs were isolated by enzymatic and outgrowth methods (40), with careful attention given to the complete removal of the adventitial layer. Dissected pieces of aortic media were minced and incubated with collagenase (1 mg/ml) and elastase type III (0.125 mg/ml) in DMEM for 30 min. Tissues were plated with DMEM containing 10% FBS. Cell numbers were determined 24 to 96 hours later. Cellular stress was induced by incubating VSMCs with H2O2 at 375 μM and 750 μM for 1 hour. The apoptotic rate was determined 6 hours after incubation with H2O2. Apoptosis was induced with 10 and 50 ng/ml recombinant FasL (R&D Systems) for 12 hours in the presence of 10 μg/ml of a cross-linking antibody (mouse anti-6X histidine; R&D Systems). Annexin V staining was performed according to the manufacturer’s protocol (BD Biosciences — Pharmingen). STAT3 activation and inhibition was performed in low-serum cultured (0.5% FBS) VSMCs. Cells were incubated with 40 ng/ml LIF (Sigma-Aldrich), and SDF-1 mRNA expression was analyzed at the indicated time points. LIF-stimulated VSMCs were incubated with the STAT3 activation inhibitor PpYLKTK-mts (Calbiochem) at the indicated concentrations and SDF-1 mRNA expression analyzed 4 hours later.
RNA was extracted from blood vessels using the Qiagen RNeasy kit. A total of 2 μg total RNA was annealed to 1 μg of random primers at 70°C, and first-strand cDNA synthesis was performed in a 25-μl reaction volume following the Promega protocol for M-MLV reverse transcriptase. p21Cip1, p53, and SDF-1 expression levels were analyzed by real-time PCR using the DyNAmo HS SYBR Green qPCR kit (New England Biolabs Inc.), with 0.2 μl from each cDNA synthesis (or the equivalent cDNA from 15 ng of RNA) and a final primer concentration of 0.3 μM in each 20-μl PCR reaction. Real-time PCR was performed with an initial 10-min cycle at 95°C (denaturation), followed by 40 amplification cycles as follows: 10 s at 95°C (denaturation), 20 s at 60°C (annealing), and 30 s at 72°C (extension). Primer sequences are available in Supplemental Methods.
p21–/– and p21+/+ mice were investigated using an established model of femoral artery wire injury, which results in complete endothelial denudation (66). This procedure was performed by a surgeon who was blinded to the mouse genotype. BrdU (25 mg/kg) was injected at 24 hours and 1 hour prior to tissue harvest. The percent of BrdU-positive cells in the intima and intima/media area ratio were analyzed by computer-assisted morphometry (n = 5 mice, 10 arteries/group). For each mouse, 4 sections were analyzed per artery.
BM was obtained from 8- to 12-week-old p21–/– and p21+/+ mice after euthanasia with CO2. BM cell suspensions were flushed from femurs and tibias, filtered, and stored on ice until use. Recipient mice were lethally irradiated with 900 rads of whole-body irradiation and then received 5 × 106 unfractionated BM cells by intravenous tail vein injection. Successful engraftment was confirmed 12 weeks later by quantitative PCR for the presence or absence of p21Cip1 or Sry to distinguish female and male BM cells (n = 5 mice, 10 arteries/group).
Immunohistochemistry was performed on paraffin-embedded tissues using an ABC immunoperoxidase protocol including antigen retrieval. The following primary antibodies were used: anti–smooth muscle α-actin antibody (1:1000, 1A4; Roche), F4/80 antibody against macrophages (1:100, A3-1; Serotec), 7/4 antibody against neutrophils (1:10, 7/4; Cedarlane), CD3 antibody against T lymphocytes (1:100; DAKO Cytomation), and alkaline phosphatase-conjugated mouse monoclonal antibody against BrdU (1 U/ml; Roche). Immunofluorescence staining was performed on fresh frozen tissue sections without antigen retrieval. Methanol/acetone-fixed sections were stained with primary antibodies against SDF-1 (1:100; eBioscience), CD31 (1:100, MEC 13.3; BD Biosciences — Pharmingen), CD45 (1:50, 30-F11; eBioscience), F4/80 (1:100; Invitrogen), p21Cip1 (1:50, ab2961; Abcam Inc.), p53 (1:50, sc-6243; Santa Cruz Biotechnology Inc.), and smooth muscle α-actin (FITC-conjugated) (1:100, 1A4; Sigma-Aldrich). Where appropriate, these were used in conjunction with Alexa Fluor 488– or Alexa Fluor 594–conjugated secondary antibodies (1:200 dilution; Invitrogen). Apoptotic vascular cells were identified by TUNEL assay. Images were acquired using either a Zeiss Axioskop plus light microscope with AxioVision V4.3 software or, for confocal microscopy, a Zeiss LSM 510 UV laser scanning confocal microscope system (Carl Zeiss GmbH). Cell counting (minimum 100 cells/section) was performed by a blinded member of our laboratory with extensive experience in both cell morphology and the techniques described above. Western blot analysis was performed on homogenized arterial and VSMC samples in RIPA lysis buffer using antibodies against mouse p21Cip1 (sc397, 1:1000; Santa Cruz Biotechnology Inc.), STAT3 (1:1000; Cell Signaling Technology), and p-STAT3 (1:1000; Cell Signaling Technology). Each lane was loaded with 50 μg protein (Bradford assay). For immunoprecipitation, cells were lysed in NP-40 buffer (75 mM NaCl, 1.0% NP-40, 50 mM Tris, pH 8.0, and protease inhibitors). Cell lysate (200 μg) was incubated with the antibodies indicated in Figure Figure2A2A and Figure Figure8D8D and/or with isotype control IgG and analyzed by western blot. Arterial cytokine concentrations were measured using a mouse SearchLight Proteome Array (Pierce Biotechnology).
Commencing 24 hours prior to vascular injury, a continuous 20-mg/ml dose of AMD3100 (Sigma-Aldrich) was administered to mice via osmotic minipump (Alzet) at a rate of 2 mg/wk for 2 weeks. CXCR4 blocking antibody (clone 2B11; BD Biosciences) was administered intravenously commencing 24 hours prior to vascular injury at 3-day intervals for 1 or 2 weeks at a dose of 20 μg. IgG2b rat isotype antibody was used as a control for this experiment (clone A95-1; BD Biosciences) (48).
Peripheral blood was isolated from mice by cardiac puncture before and 7 days after injury. Complete blood cell counts were performed using a standard automated cell counting hematology analyzer (Cell-dyne 3500; Abbott). Automated image identification for cell counting was performed. Inflammatory cell phenotyping was performed using 200 μl of blood lysed with ACK buffer for 15 minutes, incubated with Fc Block (BD Biosciences — Pharmingen), and then stained with fluorochrome-conjugated antibodies against CD11b (M1/70), CD62L (2.4G2), and Ly-6C/G (RB6-8C5) (all BD Biosciences — Pharmingen) for 30 minutes. We gated monocytes as CD11b+ and side-scatter low and determined the percentage of Ly-6C/G+/CD62L+ and Ly-6C/G–/CD62L– monocytes. Automatic compensation was performed based on single-stained samples for the respective antibodies. The stained cells were analyzed on a BD FACSCanto using Diva Software.
Formalin-crosslinked protein-DNA complexes in cell and tissue lysates were sonicated to an average size of ~500 bp. Complexes were pre-cleared and immunoprecipitated using protein A/G agarose beads (Zymed Laboratories) and a STAT3 antibody (Santa Cruz Biotechnology Inc.) or isotype controls. Subsequently, crosslinking was reversed by overnight incubation at 65°C and proteinase K treatment. The enriched specific promoter fragments were measured by quantitative PCR. SDF-1 primer sequences are available in Supplemental Methods. The relative SDF-1 promoter occupancy was adjusted to the background content of the unrelated β-actin promoter sequence, isotype IgG controls, and the initial sonicated chromatin input.
The potential STAT consensus sequence (TTCCCGGGAA) within the SDF-1 promoter was mutated to AGCCCGGGCT (SDF-mut) using the Quickchange mutagenesis II kit (Stratagene). Murine p21–/– and p21+/+ VSMCs were transfected with 2 μg of the indicated plasmids (pGL3 backbone) and a control pRL-SV40 plasmid using the Amaxa Nucleofactor Kit (Amaxa) according to the manufacturer’s instructions. Luciferase activity was quantified 24 hours after transfection using the dual luciferase assay system (Promega) and was normalized to the expression of Renilla luciferase.
Numbers of mice used for each experiment are provided in the figure legends. For cell culture and immunohistochemistry, each individual experiment was repeated 3 times. Experimental data were analyzed by ANOVA followed by Dunn correction or unpaired 2-tailed t test. Results are expressed as mean ± SEM.
We thank Tyler Jacks for kindly providing the p21Cip1 knockout mice and Giovanna Tosato for sharing reagents. We thank the staff of the Laboratory of Animal Medicine and Surgery and Robin Schwartzbeck for their assistance with the transgenic mice. We also acknowledge the professional skills and advice of Christian A. Combs and Daniela Malide (Light Microscopy Core Facility, National Heart, Lung and Blood Institute) and J. Philip McCoy (Flow Cytometry Core Facility, National Heart, Lung and Blood Institute). This project was funded by the intramural research program of the National Heart, Lung, and Blood Institute.
Nonstandard abbreviations used: LIF, leukemia inhibitory factor; SDF-1, stromal cell–derived factor-1.
Conflict of interest: The authors have declared that no conflict of interest exists.
Citation for this article: J. Clin. Invest. 118:2050–2061 (2008). doi:10.1172/JCI31244