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J Virol. 1994 December; 68(12): 8454–8460.
PMCID: PMC237322

Infection of a yellow baboon with simian immunodeficiency virus from African green monkeys: evidence for cross-species transmission in the wild.


Many African primates are known to be naturally infected with simian immunodeficiency viruses (SIVs), but only a fraction of these viruses has been molecularly characterized. One primate species for which only serological evidence of SIV infection has been reported is the yellow baboon (Papio hamadryas cynocephalus). Two wild-living baboons with strong SIVAGM seroreactivity were previously identified in a Tanzanian national park where baboons and African green monkeys shared the same habitat (T. Kodama, D. P. Silva, M. D. Daniel, J. E. Phillips-Conroy, C. J. Jolly, J. Rogers, and R. C. Desrosiers, AIDS Res. Hum. Retroviruses 5:337-343, 1989). To determine the genetic identity of the viruses infecting these animals, we used PCR to examine SIV sequences directly in uncultured leukocyte DNA. Targeting two different, nonoverlapping genomic regions, we amplified and sequenced a 673-bp gag gene fragment and a 908-bp env gene fragment from one of the two baboons. Phylo-genetic analyses revealed that this baboon was infected with an SIVAGM strain of the vervet subtype. These results provide the first direct evidence for simian-to-simian cross-species transmission of SIV in the wild.

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  • Allan JS, Kanda P, Kennedy RC, Cobb EK, Anthony M, Eichberg JW. Isolation and characterization of simian immunodeficiency viruses from two subspecies of African green monkeys. AIDS Res Hum Retroviruses. 1990 Mar;6(3):275–285. [PubMed]
  • Allan JS, Short M, Taylor ME, Su S, Hirsch VM, Johnson PR, Shaw GM, Hahn BH. Species-specific diversity among simian immunodeficiency viruses from African green monkeys. J Virol. 1991 Jun;65(6):2816–2828. [PMC free article] [PubMed]
  • Benveniste RE, Hill RW, Knott WB, Tsai CC, Kuller L, Morton WR. Detection of serum antibodies in Ethiopian baboons that cross-react with SIV, HTLV-I, and type D retroviral antigens. J Med Primatol. 1993 Feb-May;22(2-3):124–128. [PubMed]
  • Castro BA, Nepomuceno M, Lerche NW, Eichberg JW, Levy JA. Persistent infection of baboons and rhesus monkeys with different strains of HIV-2. Virology. 1991 Sep;184(1):219–226. [PubMed]
  • Daniel MD, Letvin NL, King NW, Kannagi M, Sehgal PK, Hunt RD, Kanki PJ, Essex M, Desrosiers RC. Isolation of T-cell tropic HTLV-III-like retrovirus from macaques. Science. 1985 Jun 7;228(4704):1201–1204. [PubMed]
  • Desrosiers RC. The simian immunodeficiency viruses. Annu Rev Immunol. 1990;8:557–578. [PubMed]
  • Estaquier J, Peeters M, Bedjabaga L, Honoré C, Bussi P, Dixson A, Delaporte E. Prevalence and transmission of simian immunodeficiency virus and simian T-cell leukemia virus in a semi-free-range breeding colony of mandrills in Gabon. AIDS. 1991 Nov;5(11):1385–1386. [PubMed]
  • Fukasawa M, Miura T, Hasegawa A, Morikawa S, Tsujimoto H, Miki K, Kitamura T, Hayami M. Sequence of simian immunodeficiency virus from African green monkey, a new member of the HIV/SIV group. Nature. 1988 Jun 2;333(6172):457–461. [PubMed]
  • Gao F, Yue L, Robertson DL, Hill SC, Hui H, Biggar RJ, Neequaye AE, Whelan TM, Ho DD, Shaw GM, et al. Genetic diversity of human immunodeficiency virus type 2: evidence for distinct sequence subtypes with differences in virus biology. J Virol. 1994 Nov;68(11):7433–7447. [PMC free article] [PubMed]
  • Gao F, Yue L, White AT, Pappas PG, Barchue J, Hanson AP, Greene BM, Sharp PM, Shaw GM, Hahn BH. Human infection by genetically diverse SIVSM-related HIV-2 in west Africa. Nature. 1992 Aug 6;358(6386):495–499. [PubMed]
  • Gürtler LG, Hauser PH, Eberle J, von Brunn A, Knapp S, Zekeng L, Tsague JM, Kaptue L. A new subtype of human immunodeficiency virus type 1 (MVP-5180) from Cameroon. J Virol. 1994 Mar;68(3):1581–1585. [PMC free article] [PubMed]
  • Hainsey BM, Hubbard GB, Leland MM, Brasky KM. Clinical parameters of the normal baboons (Papio species) and chimpanzees (Pan troglodytes). Lab Anim Sci. 1993 Jun;43(3):236–243. [PubMed]
  • Hendry RM, Wells MA, Phelan MA, Schneider AL, Epstein JS, Quinnan GV. Antibodies to simian immunodeficiency virus in African green monkeys in Africa in 1957-62. Lancet. 1986 Aug 23;2(8504):455–455. [PubMed]
  • Higgins DG, Bleasby AJ, Fuchs R. CLUSTAL V: improved software for multiple sequence alignment. Comput Appl Biosci. 1992 Apr;8(2):189–191. [PubMed]
  • Hirsch VM, Dapolito GA, Goldstein S, McClure H, Emau P, Fultz PN, Isahakia M, Lenroot R, Myers G, Johnson PR. A distinct African lentivirus from Sykes' monkeys. J Virol. 1993 Mar;67(3):1517–1528. [PMC free article] [PubMed]
  • Hirsch VM, Olmsted RA, Murphey-Corb M, Purcell RH, Johnson PR. An African primate lentivirus (SIVsm) closely related to HIV-2. Nature. 1989 Jun 1;339(6223):389–392. [PubMed]
  • Huet T, Cheynier R, Meyerhans A, Roelants G, Wain-Hobson S. Genetic organization of a chimpanzee lentivirus related to HIV-1. Nature. 1990 May 24;345(6273):356–359. [PubMed]
  • Jin MJ, Hui H, Robertson DL, Müller MC, Barré-Sinoussi F, Hirsch VM, Allan JS, Shaw GM, Sharp PM, Hahn BH. Mosaic genome structure of simian immunodeficiency virus from west African green monkeys. EMBO J. 1994 Jun 15;13(12):2935–2947. [PubMed]
  • Khan AS, Galvin TA, Lowenstine LJ, Jennings MB, Gardner MB, Buckler CE. A highly divergent simian immunodeficiency virus (SIVstm) recovered from stored stump-tailed macaque tissues. J Virol. 1991 Dec;65(12):7061–7065. [PMC free article] [PubMed]
  • King NW, Chalifoux LV, Ringler DJ, Wyand MS, Sehgal PK, Daniel MD, Letvin NL, Desrosiers RC, Blake BJ, Hunt RD. Comparative biology of natural and experimental SIVmac infection in macaque monkeys: a review. J Med Primatol. 1990;19(2):109–118. [PubMed]
  • Kodama T, Silva DP, Daniel MD, Phillips-Conroy JE, Jolly CJ, Rogers J, Desrosiers RC. Prevalence of antibodies to SIV in baboons in their native habitat. AIDS Res Hum Retroviruses. 1989 Jun;5(3):337–343. [PubMed]
  • Li WH. Unbiased estimation of the rates of synonymous and nonsynonymous substitution. J Mol Evol. 1993 Jan;36(1):96–99. [PubMed]
  • Li WH, Wu CI, Luo CC. A new method for estimating synonymous and nonsynonymous rates of nucleotide substitution considering the relative likelihood of nucleotide and codon changes. Mol Biol Evol. 1985 Mar;2(2):150–174. [PubMed]
  • Li Y, Naidu YM, Daniel MD, Desrosiers RC. Extensive genetic variability of simian immunodeficiency virus from African green monkeys. J Virol. 1989 Apr;63(4):1800–1802. [PMC free article] [PubMed]
  • Lowenstine LJ, Pedersen NC, Higgins J, Pallis KC, Uyeda A, Marx P, Lerche NW, Munn RJ, Gardner MB. Seroepidemiologic survey of captive Old-World primates for antibodies to human and simian retroviruses, and isolation of a lentivirus from sooty mangabeys (Cercocebus atys). Int J Cancer. 1986 Oct 15;38(4):563–574. [PubMed]
  • Marx PA, Li Y, Lerche NW, Sutjipto S, Gettie A, Yee JA, Brotman BH, Prince AM, Hanson A, Webster RG, et al. Isolation of a simian immunodeficiency virus related to human immunodeficiency virus type 2 from a west African pet sooty mangabey. J Virol. 1991 Aug;65(8):4480–4485. [PMC free article] [PubMed]
  • McKeating JA, Willey RL. Structure and function of the HIV envelope. AIDS. 1989;3 (Suppl 1):S35–S41. [PubMed]
  • Müller MC, Saksena NK, Nerrienet E, Chappey C, Hervé VM, Durand JP, Legal-Campodonico P, Lang MC, Digoutte JP, Georges AJ, et al. Simian immunodeficiency viruses from central and western Africa: evidence for a new species-specific lentivirus in tantalus monkeys. J Virol. 1993 Mar;67(3):1227–1235. [PMC free article] [PubMed]
  • Myers G, MacInnes K, Korber B. The emergence of simian/human immunodeficiency viruses. AIDS Res Hum Retroviruses. 1992 Mar;8(3):373–386. [PubMed]
  • Nathanson N, McGann KA, Wilesmith J, Desrosiers RC, Brookmeyer R. The evolution of virus diseases: their emergence, epidemicity, and control. Virus Res. 1993 Jul;29(1):3–20. [PubMed]
  • Novembre FJ, Hirsch VM, McClure HM, Fultz PN, Johnson PR. SIV from stump-tailed macaques: molecular characterization of a highly transmissible primate lentivirus. Virology. 1992 Feb;186(2):783–787. [PubMed]
  • Ohta Y, Masuda T, Tsujimoto H, Ishikawa K, Kodama T, Morikawa S, Nakai M, Honjo S, Hayami M. Isolation of simian immunodeficiency virus from African green monkeys and seroepidemiologic survey of the virus in various non-human primates. Int J Cancer. 1988 Jan 15;41(1):115–122. [PubMed]
  • Peeters M, Fransen K, Delaporte E, Van den Haesevelde M, Gershy-Damet GM, Kestens L, van der Groen G, Piot P. Isolation and characterization of a new chimpanzee lentivirus (simian immunodeficiency virus isolate cpz-ant) from a wild-captured chimpanzee. AIDS. 1992 May;6(5):447–451. [PubMed]
  • Phillips-Conroy JE, Jolly CJ, Petros B, Allan JS, Desrosiers RC. Sexual transmission of SIVagm in wild grivet monkeys. J Med Primatol. 1994 Jan;23(1):1–7. [PubMed]
  • Piatak M, Jr, Saag MS, Yang LC, Clark SJ, Kappes JC, Luk KC, Hahn BH, Shaw GM, Lifson JD. High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR. Science. 1993 Mar 19;259(5102):1749–1754. [PubMed]
  • Rogers J, Kidd KK. Nuclear DNA polymorphisms in a wild population of yellow baboons (Papio hamadryas cynocephalus) from Mikumi National Park, Tanzania. Am J Phys Anthropol. 1993 Apr;90(4):477–486. [PubMed]
  • Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. [PubMed]
  • Tomonaga K, Katahira J, Fukasawa M, Hassan MA, Kawamura M, Akari H, Miura T, Goto T, Nakai M, Suleman M, et al. Isolation and characterization of simian immunodeficiency virus from African white-crowned mangabey monkeys (Cercocebus torquatus lunulatus). Arch Virol. 1993;129(1-4):77–92. [PubMed]
  • Tözsér J, Bláha I, Copeland TD, Wondrak EM, Oroszlan S. Comparison of the HIV-1 and HIV-2 proteinases using oligopeptide substrates representing cleavage sites in Gag and Gag-Pol polyproteins. FEBS Lett. 1991 Apr 9;281(1-2):77–80. [PubMed]
  • Tsujimoto H, Hasegawa A, Maki N, Fukasawa M, Miura T, Speidel S, Cooper RW, Moriyama EN, Gojobori T, Hayami M. Sequence of a novel simian immunodeficiency virus from a wild-caught African mandrill. Nature. 1989 Oct 12;341(6242):539–541. [PubMed]
  • Vanden Haesevelde M, Decourt JL, De Leys RJ, Vanderborght B, van der Groen G, van Heuverswijn H, Saman E. Genomic cloning and complete sequence analysis of a highly divergent African human immunodeficiency virus isolate. J Virol. 1994 Mar;68(3):1586–1596. [PMC free article] [PubMed]

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