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Br J Cancer. May 1999; 80(3-4): 569–578.
Published online May 1, 1999. doi:  10.1038/sj.bjc.6690393
PMCID: PMC2362349
Characterization and distribution of an oncofetal antigen (M2A antigen) expressed on testicular germ cell tumours
A Marks,1 D R Sutherland,2 D Bailey,3 J Iglesias,1 J Law,1 M Lei,1 H Yeger,3 D Banerjee,3 and R Baumal3
Banting and Best Department of Medical Research
Departments of Medicineand Pathology, University of Toronto, 112 College Street, Toronto, Ontario, Canada M5G 1L6,
Received April 14, 1998; Revised September 30, 1998; Accepted September 28, 1998.
Abstract
M2A antigen is an oncofetal antigen associated with germ cell neoplasia, present in testis on fetal gonocytes and re-expressed on carcinoma in situ (CIS) and germ cell tumours. We developed a panel of monoclonal antibodies (mAb), M2A (IgG2a), D1-26 (IgG2b) and D2-40 (IgG1), to this antigen in order to characterize its structure and study its distribution among germ cell tumours. M2A antigen was purified by sequential lectin and antibody affinity chromatography and characterized as a monomeric Mr 40 000 surface sialoglycoprotein, extensively glycosylated with O-linked carbohydrate structures, but devoid of N-linked sugars. Terminal sialic acid residues were required for reactivity with mAb M2A and D1-26, but not D2-40. Sections of 69 testicular germ cell tumours, fixed in formalin and embedded in paraffin, were stained with mAb D2-40 to examine the distribution of M2A antigen. Uniform membrane staining was observed in seminomas, and focal staining in 69% of embryonal carcinomas, 29% of teratomas and 25% of yolk sac tumours. CIS in the vicinity of all germ cell tumours also displayed uniform membrane staining. The characterization of M2A antigen, and the development of mAb which react with it in conventionally preserved archival specimens, provide important initiatives to study the origin and progression of germ cell neoplasia. © 1999 Cancer Research Campaign
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