PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
 
J Bacteriol. 1975 April; 122(1): 257–265.
PMCID: PMC235665

Isolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases.

Abstract

Seven transfer ribonucleic acid (tRNA) methylase mutants were isolated from Escherichia coli K-12 by examining the ability of RNA prepared from clones of unselected mutagenized cells to accept methyl groups from S-adenosylmethionine catalyzed by crude enzymes from wild-type cells. Five of the mutants had an altered uracil-tRNA methylase; consequently their tRNA's lacked ribothymidine. One mutant had tRNA deficient in 7-methylguanosine, and one mutant contained tRNA lacking 2-thio-5-methylaminomethyluridine. The genetic loci of the three tRNA methylase mutants were distributed over the E. coli genome. The mutant strain deficient in 7-methylguanosine biosynthesis showed a reduced efficiency in the suppression of amber mutations carried by T4 or lambda phages.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • ADELBERG EA, BURNS SN. Genetic variation in the sex factor of Escherichia coli. J Bacteriol. 1960 Mar;79:321–330. [PMC free article] [PubMed]
  • Björk GR, Isaksson LA. Isolation of mutants of Escherichia coli lac king 5-methyluracil in transfer ribonucleic acid or 1-methylguanine in ribosomal RNA. J Mol Biol. 1970 Jul 14;51(1):83–100. [PubMed]
  • Björk GR, Neidhardt FC. Analysis of 5-methyluridine function in the transfer RNA of Escherichia coli. Cancer Res. 1971 May;31(5):706–709. [PubMed]
  • Cortese R, Kammen HO, Spengler SJ, Ames BN. Biosynthesis of pseudouridine in transfer ribonucleic acid. J Biol Chem. 1974 Feb 25;249(4):1103–1108. [PubMed]
  • Fareed GC, Richardson CC. Enzymatic breakage and joining of deoxyribonucleic acid. II. The structural gene for polynucleotide ligase in bacteriophage T4. Proc Natl Acad Sci U S A. 1967 Aug;58(2):665–672. [PubMed]
  • FLEISSNER E, BOREK E. STUDIES ON THE ENZYMATIC METHYLATION OF SOLUBLE RNA. I. METHYLATION OF THE S-RNA POLYMER. Biochemistry. 1963 Sep-Oct;2:1093–1100. [PubMed]
  • Grosjean H, Takada C, Petre J. Complex formation between transfer RNAs with complementary anticodons: use of matrix bound tRNA. Biochem Biophys Res Commun. 1973 Aug 6;53(3):882–893. [PubMed]
  • Guerola N, Ingraham JL, Cerdá-Olmedo E. Induction of closely linked multiple mutations by nitrosoguanidine. Nat New Biol. 1971 Mar 24;230(12):122–125. [PubMed]
  • Harris CL, Titchener EB, Cline AL. Sulfur-deficient transfer ribonucleic acid in a cysteine-requiring, "relaxed" mutant of Escherichia coli. J Bacteriol. 1969 Dec;100(3):1322–1327. [PMC free article] [PubMed]
  • Johnson L, Hayashi H, Söll D. Isolation and properties of a transfer ribonucleic acid deficient in ribothymidine. Biochemistry. 1970 Jul 7;9(14):2823–2831. [PubMed]
  • Kelmers AD, Heatherly DE. Columns for rapid chromatographic separation of small amounts of tracer-labeled transfer ribonucleic acids. Anal Biochem. 1971 Dec;44(2):486–495. [PubMed]
  • Kerr SJ, Borek E. The tRNA methyltransferases. Adv Enzymol Relat Areas Mol Biol. 1972;36:1–27. [PubMed]
  • Low KB. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. [PMC free article] [PubMed]
  • LURIA SE, BURROUS JW. Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957 Oct;74(4):461–476. [PMC free article] [PubMed]
  • Marinus MG. Location of DNA methylation genes on the Escherichia coli K-12 genetic map. Mol Gen Genet. 1973 Dec 14;127(1):47–55. [PubMed]
  • Marinus MG, Morris NR. Isolation of deoxyribonucleic acid methylase mutants of Escherichia coli K-12. J Bacteriol. 1973 Jun;114(3):1143–1150. [PMC free article] [PubMed]
  • Nichols JL, Lane BG. In vitro O2'-methylation of sugars in E. coli RNA. II. Methylation of ribosomal and transfer RNA by homologous methylases in crude cell-free extracts and particulate suspensions from a relaxed mutant of E. coli. Can J Biochem. 1968 Dec;46(12):1487–1495. [PubMed]
  • Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed]
  • Ohashi Ziro, Harada Fumio, Nishimura Susumu. Primary sequence of glutamic acid tRNA II from Escherichia coli. FEBS Lett. 1972 Feb 1;20(2):239–241. [PubMed]
  • Phillips JH, Kjellin-Stråby K. Studies on microbial ribonucleic acid. IV. Two mutants of Saccharomyces cerevisiae lacking N-2-dimethylguanine in soluble ribonucleic acid. J Mol Biol. 1967 Jun 28;26(3):509–518. [PubMed]
  • Schaefer KP, Altman S, Söll D. Nucleotide modification in vitro of the precursor of transfer RNA of Escherichia coli. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3626–3630. [PubMed]
  • Schäfer KP, Söll D. New aspects in tRNA biosynthesis. Biochimie. 1974;56(6-7):795–804. [PubMed]
  • Shimura Y, Ozeki H. Genetic study on transfer RNA. Adv Biophys. 1973;4:191–226. [PubMed]
  • Smith JD. Gentics of transfer RNA. Annu Rev Genet. 1972;6:235–256. [PubMed]
  • Söll D, Cherayil JD, Bock RM. Studies on polynucleotides. LXXV. Specificity of tRNA for codon recognition as studied by the ribosomal binding technique. J Mol Biol. 1967 Oct 14;29(1):97–112. [PubMed]
  • Taya Y, Nishimura S. Biosynthesis of 5-methylaminomethyl-2-thiouridylate. I. Isolation of a new tRNA-methylase specific for 5-methylaminomethyl-2-thiouridylate. Biochem Biophys Res Commun. 1973 Apr 16;51(4):1062–1068. [PubMed]
  • Taylor AL, Trotter CD. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. [PMC free article] [PubMed]
  • Yang S, Reinitz ER, Gefter ML. Role of modifications in tyrosine transfer RNA. II. Ribothymidylate-deficient tRNA. Arch Biochem Biophys. 1973 Jul;157(1):55–62. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)