PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
 
J Bacteriol. 1976 October; 128(1): 21–27.
PMCID: PMC232821

Effect of cerulenin on the growth and differentiation of Dictyostelium discoideum.

Abstract

The growth of Dictyostelium discoideum Ax-2 was inhibited completely by cerulenin at a concentration of 5 mug/ml. This inhibition of growth was found to be due to the inhibition of fatty acid synthesis. Acetate incorporation into a long-chain fatty acid was inhibited completely by cerulenin, and the growth inhibition could be reversed by inclusion of certain saturated fatty acids in the medium. Unsaturated fatty acids and sterols failed to reverse the inhibitory effect. The fatty acid and sterol compositions of cerulenin-treated cells were determined to establish whether the drug could be used to manipulate the organism's lipid composition. Only relatively small manipulations were obtained under the conditions employed in this study. Cerulenin inhibited differentiation but only at high concentrations (150 mug/ml). This inhibition could be reversed by palmitic acid, suggesting that the prime cause of the inhibition was an inhibition of fatty acid synthesis. Thus, it appears that continued fatty acid synthesis is required for the cellular process of differentiation in D. discoideum.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Cronan JE, Jr, Gelmann EP. Physical properties of membrane lipids: biological relevance and regulation. Bacteriol Rev. 1975 Sep;39(3):232–256. [PMC free article] [PubMed]
  • D'Agnolo G, Rosenfeld IS, Awaya J, Omura S, Vagelos PR. Inhibition of fatty acid synthesis by the antibiotic cerulenin. Specific inactivation of beta-ketoacyl-acyl carrier protein synthetase. Biochim Biophys Acta. 1973 Nov 29;326(2):155–156. [PubMed]
  • DAVIDOFF F, KORN ED. FATTY ACID AND PHOSPHOLIPID COMPOSITION OF THE CELLULAR SLIME MOLD, DICTYOSTELIUM DISCOIDEUM. THE OCCURRENCE OF PREVIOUSLY UNDESCRIBED FATTY ACIDS. J Biol Chem. 1963 Oct;238:3199–3209. [PubMed]
  • DAVIDOFF F, KORN ED. THE BIOSYNTHESIS OF FATTY ACIDS IN THE CELLULAR SLIME MOLD, DICTYOSTELIUM DISCOIDEUM. J Biol Chem. 1963 Oct;238:3210–3215. [PubMed]
  • Ellingson JS, Telser A, Sussman M. Regulation of functionally related enzymes during alternative developmental programs. Biochim Biophys Acta. 1971 Aug 19;244(2):388–395. [PubMed]
  • Ellouz R, Lenfant M. Biosynthèse de la chaîne latérale éthyle du stigmastanol et du stigmastèn-22,01-3beta du myxomycète Dictyostelium discoïdeum. Eur J Biochem. 1971 Dec 10;23(3):544–550. [PubMed]
  • Farías RN, Bloj B, Morero RD, Siñeriz F, Trucco RE. Regulation of allosteric membrane-bound enzymes through changes in membrane lipid compostition. Biochim Biophys Acta. 1975 Jun 30;415(2):231–251. [PubMed]
  • Ferguson KA, Glaser M, Bayer WH, Vagelos PR. Alteration of fatty acid composition of LM cells by lipid supplementation and temperature. Biochemistry. 1975 Jan 14;14(1):146–151. [PubMed]
  • Glaser M, Ferguson KA, Vagelos PR. Manipulation of the phospholipid composition of tissue culture cells. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4072–4076. [PubMed]
  • Goldberg I, Walker JR, Bloch K. Inhibition of lipid synthesis in Escherichia coli cells by the antibiotic cerulenin. Antimicrob Agents Chemother. 1973 May;3(5):549–554. [PMC free article] [PubMed]
  • HEFTMANN E, WRIGHT BE, LIDDEL GU. The isolation of Delta22-stigmasten-3beta-ol from Dictyostelium discoideum. Arch Biochem Biophys. 1960 Dec;91:266–270. [PubMed]
  • Henry SA, Keith AD. Saturated fatty acid requirer of Neurospora crassa. J Bacteriol. 1971 Apr;106(1):174–182. [PMC free article] [PubMed]
  • Hirschberg E, Ceccarini C, Osnos M, Carchman R. Effects of inhibitors of nucleic acid and protein synthesis on growth and aggregation of the cellular slime mold Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1968 Sep;61(1):316–323. [PubMed]
  • Keith AD, Resnick MR, Haley AB. Fatty acid desaturase mutants of Saccharomyces cerevisiae. J Bacteriol. 1969 May;98(2):415–420. [PMC free article] [PubMed]
  • Lees AM, Korn ED. Metabolism of unsaturated fatty acids in protozoa. Biochemistry. 1966 May;5(5):1475–1481. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • McElhaney RN, Tourtellotte ME. Mycoplasma membrane lipids: variations in fatty acid composition. Science. 1969 Apr 25;164(3878):433–434. [PubMed]
  • Nomura S, Horiuchi T, Omura S, Hata T. The action mechanism of cerulenin. I. Effect of cerulenin on sterol and fatty acid biosynthesis in yeast. J Biochem. 1972 May;71(5):783–796. [PubMed]
  • Ono T, Kesado T, Awaya J, Omura S. Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast. Biochem Biophys Res Commun. 1974 Apr 23;57(4):1119–1124. [PubMed]
  • Vance D, Goldberg I, Mitsuhashi O, Bloch K. Inhibition of fatty acid synthetases by the antibiotic cerulenin. Biochem Biophys Res Commun. 1972 Aug 7;48(3):649–656. [PubMed]
  • Weeks C, Weeks G. Cell surface changes during the differentiation of Dictyostelium discoideum. Interaction of cells with Concanavalin A. Exp Cell Res. 1975 May;92(2):372–382. [PubMed]
  • Weeks G. Agglutination of growing and differentiating cells of Dictyostelium discoideum by concanavalin A. Exp Cell Res. 1973 Feb;76(2):467–470. [PubMed]
  • Wille W, Eisenstadt E, Willecke K. Inhibition of de novo fatty acid synthesis by the antibiotic cerulenin in Bacillus subtilis: effects on citrate-Mg2+ transport and synthesis of macromolecules. Antimicrob Agents Chemother. 1975 Sep;8(3):231–237. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)