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SeqPrep technology is developed for easy and quick DNA preparation from bacterial culture. It consists of a surface-modified microplate and a cell lysis/DNA-binding solution. The DNA binds to the surface while cells get lysed. With this technology, DNA preparation is not only highly automatable but also very easy manually. Several robotic plaforms have been validated for plasmid DNA preparation with SeqPrep. Data will be presented. Optimization for individual labs’ needs will be discussed in four aspects of the preparation process: bacterial culture, cell harvest, DNA isolation and DNA elution.