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J Biomol Tech. 2007 February; 18(1): 43.
PMCID: PMC2291929

P122-M The Effect of Ion-Pairing Reagents on the Reversed Phase Purification of DMT-off Oligonucleotides


The use of ion-pairing reagents for reversed phase peptide separations has been investigated in numerous publications. Reversed phase oligonucleotide separations typically employ TEAA as an ion-pairing reagent. However, other ion-pair reagents may provide better separation results. We investigated several different ion-pairing reagents using a ten micron, mono-sized, reversed phase polymeric resin, Amberchrom HPR10. DMT-off purifications of two synthetic 12mer DNA oligonucleotides (AAA CCT GAT GTG and GGT GGT GGT GGT) were performed, and yields and purities from the different purifications were compared in order to determine the most favorable ion-pairing conditions.

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