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Detergents are commonly used in hydrophobic cell membrane protein purification and viral removal/inactivation. These detergents must eventually be removed during the purification process. A rapid detergent removal protocol using short chromatographic columns has been developed. Polystyrene divinylbenzene polymers, packed in chromatography columns, were employed for the removal of non-ionic, zwitterionic, and anionic detergents from protein solutions. The results demonstrate that this resin exhibited high capacity and was effective in removing a wide range of detergents at high linear velocities (>600cm/hr) with minimal loss in protein yield.