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When performing large-fragment analysis applications on capillary electrophoresis, a high-performance size standard is essential. Applications such as AFLP, T-RFLP, VNTR, mutation screening, and BAC fingerprinting commonly generate fragments greater than 600 base pairs. Using the Applied Biosystems 5th dye technology, we have developed a new high-density size standard containing over 65 peaks, spaced no more than 40 base pairs apart. This new size standard provides accurate and precise fragment sizing from 20 to1200 base pairs, allowing the flexibility to analyze small and large fragments. We present details about the size standard and illustrate its versatility, accuracy, and precision with a variety of large-fragment analysis applications.