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J Biomol Tech. 2007 February; 18(1): 20.
PMCID: PMC2291817

P59-M Vydac MS RP-HPLC Columns Provide Unique Selectivity and High Recovery for Peptide and Protein Separations

Abstract

Based on specially treated large-pore silica and enhanced with a proprietary bonding process, Vydac MS reversed-phase (RP) high-performance liquid chromatography columns offer superior performance for peptides and proteins. The deamidation of human growth hormone (HGH) has been monitored for many years by RP using Vydac columns. The Vydac MS C4 column provides the best overall performance characteristics (recovery, resolution, and peak symmetry) for the common important assay of HGH and desamido HGH. Although hydrophobic membrane proteins are particularly difficult to separate, the Vydac MS C4 column provides better separation and recovery (up to 86% higher than other leading columns) for a reptilian reovirus p14 protein and myristolyated form, a component of a potentially new vaccine-delivery system. Separation of the trypsin digest of fetuin, a glycoprotein, exhibits improved selectivity for peptide mapping on a Vydac MS C18 column compared to other C18 columns, revealing some peaks otherwise not seen. The improved selectivity for peptides on the Vydac MS columns results in better primary structure definition and easier identification of degradation products and other protein characteristics.


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