Search tips
Search criteria 


Logo of jbtJBT IndexAssociation Homepage
J Biomol Tech. 1999 March; 10(1): 17–20.
PMCID: PMC2291582

Mass spectrometry detection and reduction of disulfide adducts between reducing agents and recombinant proteins with highly reactive cysteines

Full Text

The Full Text of this article is available as a PDF (81K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • DeSilva TM, Harper SL, Kotula L, Hensley P, Curtis PJ, Otvos L, Jr, Speicher DW. Physical properties of a single-motif erythrocyte spectrin peptide: a highly stable independently folding unit. Biochemistry. 1997 Apr 1;36(13):3991–3997. [PubMed]
  • Shih IM, Elder DE, Speicher D, Johnson JP, Herlyn M. Isolation and functional characterization of the A32 melanoma-associated antigen. Cancer Res. 1994 May 1;54(9):2514–2520. [PubMed]
  • Shih IM, Speicher D, Hsu MY, Levine E, Herlyn M. Melanoma cell-cell interactions are mediated through heterophilic Mel-CAM/ligand adhesion. Cancer Res. 1997 Sep 1;57(17):3835–3840. [PubMed]
  • Schuhmacher M, Glocker MO, Wunderlin M, Przybylski M. Direct isolation of proteins from sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analysis by electrospray-ionization mass spectrometry. Electrophoresis. 1996 May;17(5):848–854. [PubMed]
  • Han JC, Han GY. A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol. Anal Biochem. 1994 Jul;220(1):5–10. [PubMed]

Articles from Journal of Biomolecular Techniques : JBT are provided here courtesy of The Association of Biomolecular Resource Facilities