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J Cell Biol. 1992 March 2; 116(6): 1421–1430.
PMCID: PMC2289383

Protein phosphatase type 1 in mammalian cell mitosis: chromosomal localization and involvement in mitotic exit

Abstract

We have examined the role of protein phosphatase type 1 (PP-1) in mammalian cell mitosis. Immunofluorescence using anti-PP-1 antibodies revealed that PP-1, which is mainly localized in the cytoplasm of G1 and S phase cells, accumulates in the nucleus during G2 phase and intensely colocalizes with individual chromosomes at mitosis. This increase in nuclear PP-1 in G2/M cells was confirmed by immunoblotting on subcellular fractions. Microinjection of neutralizing anti-PP-1 antibodies before division blocked cells at metaphase, whereas injection of PP-1 in one pole of an anaphase B cell accelerated cytokinesis and the reflattening of the injected cell. These results reveal a specific cell cycle-dependent redistribution of PP-1 and its involvement in reversing p34cdc2-induced effects after mid-mitosis in mammalian cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Axton JM, Dombrádi V, Cohen PT, Glover DM. One of the protein phosphatase 1 isoenzymes in Drosophila is essential for mitosis. Cell. 1990 Oct 5;63(1):33–46. [PubMed]
  • Bialojan C, Takai A. Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics. Biochem J. 1988 Nov 15;256(1):283–290. [PubMed]
  • Booher R, Beach D. Involvement of a type 1 protein phosphatase encoded by bws1+ in fission yeast mitotic control. Cell. 1989 Jun 16;57(6):1009–1016. [PubMed]
  • Brautigan DL, Shriner CL, Gruppuso PA. Phosphorylase phosphatase catalytic subunit. Evidence that the Mr = 33,000 enzyme fragment is derived from a native protein of Mr = 70,000. J Biol Chem. 1985 Apr 10;260(7):4295–4302. [PubMed]
  • Brautigan DL, Gruppuso PA, Mumby M. Protein phosphatase type-1 and type-2 catalytic subunits both bind inhibitor-2 and monoclonal immunoglobulins. J Biol Chem. 1986 Nov 15;261(32):14924–14928. [PubMed]
  • Brautigan DL, Sunwoo J, Labbé JC, Fernandez A, Lamb NJ. Cell cycle oscillation of phosphatase inhibitor-2 in rat fibroblasts coincident with p34cdc2 restriction. Nature. 1990 Mar 1;344(6261):74–78. [PubMed]
  • Chisholm AA, Cohen P. Identification of a third form of protein phosphatase 1 in rabbit skeletal muscle that is associated with myosin. Biochim Biophys Acta. 1988 Mar 11;968(3):392–400. [PubMed]
  • Cohen P. The structure and regulation of protein phosphatases. Annu Rev Biochem. 1989;58:453–508. [PubMed]
  • Dombrádi V, Axton JM, Glover DM, Cohen PT. Cloning and chromosomal localization of Drosophila cDNA encoding the catalytic subunit of protein phosphatase 1 alpha. High conservation between mammalian and insect sequences. Eur J Biochem. 1989 Aug 15;183(3):603–610. [PubMed]
  • Doonan JH, Morris NR. The bimG gene of Aspergillus nidulans, required for completion of anaphase, encodes a homolog of mammalian phosphoprotein phosphatase 1. Cell. 1989 Jun 16;57(6):987–996. [PubMed]
  • Fernandez A, Brautigan DL, Mumby M, Lamb NJ. Protein phosphatase type-1, not type-2A, modulates actin microfilament integrity and myosin light chain phosphorylation in living nonmuscle cells. J Cell Biol. 1990 Jul;111(1):103–112. [PMC free article] [PubMed]
  • Fiol CJ, Haseman JH, Wang YH, Roach PJ, Roeske RW, Kowalczuk M, DePaoli-Roach AA. Phosphoserine as a recognition determinant for glycogen synthase kinase-3: phosphorylation of a synthetic peptide based on the G-component of protein phosphatase-1. Arch Biochem Biophys. 1988 Dec;267(2):797–802. [PubMed]
  • Gruppuso PA, Johnson GL, Constantinides M, Brautigan DL. Phosphorylase phosphatase regulatory subunit. "Western" blotting with immunoglobulins against inhibitor-2 reveals a protein of Mr = 60,000. J Biol Chem. 1985 Apr 10;260(7):4288–4294. [PubMed]
  • Hiraga A, Cohen P. Phosphorylation of the glycogen-binding subunit of protein phosphatase-1G by cyclic-AMP-dependent protein kinase promotes translocation of the phosphatase from glycogen to cytosol in rabbit skeletal muscle. Eur J Biochem. 1986 Dec 15;161(3):763–769. [PubMed]
  • Hubbard MJ, Cohen P. Regulation of protein phosphatase-1G from rabbit skeletal muscle. 2. Catalytic subunit translocation is a mechanism for reversible inhibition of activity toward glycogen-bound substrates. Eur J Biochem. 1989 Dec 22;186(3):711–716. [PubMed]
  • Hyman AA, Mitchison TJ. Two different microtubule-based motor activities with opposite polarities in kinetochores. Nature. 1991 May 16;351(6323):206–211. [PubMed]
  • Jakes S, Mellgren RL, Schlender KK. Isolation and characterization of an inhibitor-sensitive and a polycation-stimulated protein phosphatase from rat liver nuclei. Biochim Biophys Acta. 1986 Aug 29;888(1):135–142. [PubMed]
  • Johansen JW, Ingebritsen TS. Phosphorylation and inactivation of protein phosphatase 1 by pp60v-src. Proc Natl Acad Sci U S A. 1986 Jan;83(2):207–211. [PubMed]
  • Karess RE, Chang XJ, Edwards KA, Kulkarni S, Aguilera I, Kiehart DP. The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila. Cell. 1991 Jun 28;65(7):1177–1189. [PubMed]
  • Kinoshita N, Ohkura H, Yanagida M. Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycle. Cell. 1990 Oct 19;63(2):405–415. [PubMed]
  • Kuret J, Bell H, Cohen P. Identification of high levels of protein phosphatase-1 in rat liver nuclei. FEBS Lett. 1986 Jul 28;203(2):197–202. [PubMed]
  • Lamb NJ, Fernandez A, Watrin A, Labbé JC, Cavadore JC. Microinjection of p34cdc2 kinase induces marked changes in cell shape, cytoskeletal organization, and chromatin structure in mammalian fibroblasts. Cell. 1990 Jan 12;60(1):151–165. [PubMed]
  • Lamb NJ, Cavadore JC, Labbe JC, Maurer RA, Fernandez A. Inhibition of cAMP-dependent protein kinase plays a key role in the induction of mitosis and nuclear envelope breakdown in mammalian cells. EMBO J. 1991 Jun;10(6):1523–1533. [PubMed]
  • Lee MG, Nurse P. Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature. 1987 May 7;327(6117):31–35. [PubMed]
  • Lohka MJ. Mitotic control by metaphase-promoting factor and cdc proteins. J Cell Sci. 1989 Feb;92(Pt 2):131–135. [PubMed]
  • Moreno S, Hayles J, Nurse P. Regulation of p34cdc2 protein kinase during mitosis. Cell. 1989 Jul 28;58(2):361–372. [PubMed]
  • Ohkura H, Yanagida M. S. pombe gene sds22+ essential for a midmitotic transition encodes a leucine-rich repeat protein that positively modulates protein phosphatase-1. Cell. 1991 Jan 11;64(1):149–157. [PubMed]
  • Ohkura H, Kinoshita N, Miyatani S, Toda T, Yanagida M. The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases. Cell. 1989 Jun 16;57(6):997–1007. [PubMed]
  • Olivier AR, Ballou LM, Thomas G. Differential regulation of S6 phosphorylation by insulin and epidermal growth factor in Swiss mouse 3T3 cells: insulin activation of type 1 phosphatase. Proc Natl Acad Sci U S A. 1988 Jul;85(13):4720–4724. [PubMed]
  • Strålfors P, Hiraga A, Cohen P. The protein phosphatases involved in cellular regulation. Purification and characterisation of the glycogen-bound form of protein phosphatase-1 from rabbit skeletal muscle. Eur J Biochem. 1985 Jun 3;149(2):295–303. [PubMed]

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