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J Clin Microbiol. 1995 June; 33(6): 1452–1455.
PMCID: PMC228194

Expression, self-assembly, and antigenicity of a snow mountain agent-like calicivirus capsid protein.

Abstract

Virus-like particles were produced in insect cells infected with a recombinant baculovirus containing the capsid gene of MX virus, a Mexican strain of human calicivirus. These recombinant MX (rMX) particles were morphologically similar to recombinant Norwalk virus (rNV) particles as observed under an electron microscope and contained a single capsid protein with a molecular weight of 57,000, which was slightly smaller than that of rNV. This protein was immunoprecipitated by sera from volunteers infected with the Snow Mountain agent, but it reacted weakly with sera from volunteers infected with NV. This protein did not react with hyperimmune antisera from animals immunized with rNV in the rNV antigen enzyme immunoassay (EIA). Seroresponses were detected from volunteers infected with Snow Mountain agent and Hawaii agent when the rMX particles were used as antigen in an EIA. This EIA also detected an immune response in the sera of child from whom the MX virus was isolated, and a high prevalence of antibody to MX virus was found in the sera of a cohort of Mexican children.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Cubitt WD, Jiang XJ, Wang J, Estes MK. Sequence similarity of human caliciviruses and small round structured viruses. J Med Virol. 1994 Jul;43(3):252–258. [PubMed]
  • Dolin R, Reichman RC, Roessner KD, Tralka TS, Schooley RT, Gary W, Morens D. Detection by immune electron microscopy of the Snow Mountain agent of acute viral gastroenteritis. J Infect Dis. 1982 Aug;146(2):184–189. [PubMed]
  • Graham DY, Jiang X, Tanaka T, Opekun AR, Madore HP, Estes MK. Norwalk virus infection of volunteers: new insights based on improved assays. J Infect Dis. 1994 Jul;170(1):34–43. [PubMed]
  • Gray JJ, Jiang X, Morgan-Capner P, Desselberger U, Estes MK. Prevalence of antibodies to Norwalk virus in England: detection by enzyme-linked immunosorbent assay using baculovirus-expressed Norwalk virus capsid antigen. J Clin Microbiol. 1993 Apr;31(4):1022–1025. [PMC free article] [PubMed]
  • Hayashi Y, Ando T, Utagawa E, Sekine S, Okada S, Yabuuchi K, Miki T, Ohashi M. Western blot (immunoblot) assay of small, round-structured virus associated with an acute gastroenteritis outbreak in Tokyo. J Clin Microbiol. 1989 Aug;27(8):1728–1733. [PMC free article] [PubMed]
  • Xi JN, Graham DY, Wang KN, Estes MK. Norwalk virus genome cloning and characterization. Science. 1990 Dec 14;250(4987):1580–1583. [PubMed]
  • Jiang X, Wang J, Graham DY, Estes MK. Detection of Norwalk virus in stool by polymerase chain reaction. J Clin Microbiol. 1992 Oct;30(10):2529–2534. [PMC free article] [PubMed]
  • Jiang X, Wang M, Graham DY, Estes MK. Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein. J Virol. 1992 Nov;66(11):6527–6532. [PMC free article] [PubMed]
  • Jiang X, Wang M, Wang K, Estes MK. Sequence and genomic organization of Norwalk virus. Virology. 1993 Jul;195(1):51–61. [PubMed]
  • Lew JF, Kapikian AZ, Jiang X, Estes MK, Green KY. Molecular characterization and expression of the capsid protein of a Norwalk-like virus recovered from a Desert Shield troop with gastroenteritis. Virology. 1994 Apr;200(1):319–325. [PubMed]
  • Lew JF, Petric M, Kapikian AZ, Jiang X, Estes MK, Green KY. Identification of minireovirus as a Norwalk-like virus in pediatric patients with gastroenteritis. J Virol. 1994 May;68(5):3391–3396. [PMC free article] [PubMed]
  • Lew JF, Valdesuso J, Vesikari T, Kapikian AZ, Jiang X, Estes MK, Green KY. Detection of Norwalk virus or Norwalk-like virus infections in Finnish infants and young children. J Infect Dis. 1994 Jun;169(6):1364–1367. [PubMed]
  • Lewis DC. Three serotypes of Norwalk-like virus demonstrated by solid-phase immune electron microscopy. J Med Virol. 1990 Jan;30(1):77–81. [PubMed]
  • Lewis D. Norwalk agent and other small-round structured viruses in the U.K. J Infect. 1991 Sep;23(2):220–222. [PubMed]
  • Madore HP, Treanor JJ, Buja R, Dolin R. Antigenic relatedness among the Norwalk-like agents by serum antibody rises. J Med Virol. 1990 Oct;32(2):96–101. [PubMed]
  • Matson DO, Zhong WM, Nakata S, Numata K, Jiang X, Pickering LK, Chiba S, Estes MK. Molecular characterization of a human calicivirus with sequence relationships closer to animal caliciviruses than other known human caliciviruses. J Med Virol. 1995 Feb;45(2):215–222. [PubMed]
  • Moe CL, Gentsch J, Ando T, Grohmann G, Monroe SS, Jiang X, Wang J, Estes MK, Seto Y, Humphrey C, et al. Application of PCR to detect Norwalk virus in fecal specimens from outbreaks of gastroenteritis. J Clin Microbiol. 1994 Mar;32(3):642–648. [PMC free article] [PubMed]
  • Numata K, Nakata S, Jiang X, Estes MK, Chiba S. Epidemiological study of Norwalk virus infections in Japan and Southeast Asia by enzyme-linked immunosorbent assays with Norwalk virus capsid protein produced by the baculovirus expression system. J Clin Microbiol. 1994 Jan;32(1):121–126. [PMC free article] [PubMed]
  • Oishi I, Yamazaki K, Kimoto T, Minekawa Y. Demonstration of low molecular weight polypeptides associated with small, round-structured viruses by western immunoblot analysis. Microbiol Immunol. 1992;36(10):1105–1112. [PubMed]
  • Okada S, Sekine S, Ando T, Hayashi Y, Murao M, Yabuuchi K, Miki T, Ohashi M. Antigenic characterization of small, round-structured viruses by immune electron microscopy. J Clin Microbiol. 1990 Jun;28(6):1244–1248. [PMC free article] [PubMed]
  • Parker S, Cubitt D, Jiang JX, Estes M. Efficacy of a recombinant Norwalk virus protein enzyme immunoassay for the diagnosis of infections with Norwalk virus and other human "candidate" caliciviruses. J Med Virol. 1993 Nov;41(3):179–184. [PubMed]
  • Taniguchi K, Urasawa S, Urasawa T. Further studies of 35--40 nm virus-like particles associated with outbreaks of acute gastroenteritis. J Med Microbiol. 1981 Feb;14(1):107–118. [PubMed]
  • Treanor JJ, Jiang X, Madore HP, Estes MK. Subclass-specific serum antibody responses to recombinant Norwalk virus capsid antigen (rNV) in adults infected with Norwalk, Snow Mountain, or Hawaii virus. J Clin Microbiol. 1993 Jun;31(6):1630–1634. [PMC free article] [PubMed]
  • Raúl Velázquez F, Calva JJ, Lourdes Guerrero M, Mass D, Glass RI, Pickering LK, Ruiz-Palacios GM. Cohort study of rotavirus serotype patterns in symptomatic and asymptomatic infections in Mexican children. Pediatr Infect Dis J. 1993 Jan;12(1):54–61. [PubMed]
  • Wang J, Jiang X, Madore HP, Gray J, Desselberger U, Ando T, Seto Y, Oishi I, Lew JF, Green KY, et al. Sequence diversity of small, round-structured viruses in the Norwalk virus group. J Virol. 1994 Sep;68(9):5982–5990. [PMC free article] [PubMed]

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