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J Biomol Tech. 2003 June; 14(2): 156.
PMCID: PMC2279910


The Amino Acid Analysis Research Group (AAARG) has continued its AAA2001 study on the quantitation of proteins by amino acid analysis and dye-binding assays. The aim of the AAA2001 study was to compare the quantification of four protein solutions by AAA and by colorimetric methods in order to document the correlation between the two methods. From the 20 participating laboratories (corresponding to 54% of the laboratories that requested samples), 11 laboratories performed dye-binding assays. With regard to calibration, the dye-binding assays were not standardized. Almost every laboratory prepared the protein standards for calibration by their own procedure, making a conclusive comparison of the results difficult. It is this nonstandardized aspect that we hoped to eliminate with the AAA2003 study.

For the AAA2003 study we sent out aliquots of five protein solutions to be analyzed by AAA and by a dye-binding assay, and one aliquot of the NIST BSA to be used as the reference standard for the dye-binding assay. In order to increase the statistical significance of the study, participants were asked to analyze the samples in triplicate. Laboratories not performing AAA were also invited to participate.

The study was also open to nonmembers. From the 40 laboratories requesting samples, 28 (70%) submitted 57 AAA data sets, ten BCA data sets, four Bradford data sets, and two data sets for other dye-binding assays.

The results were presented at the ABRF2003 as a poster, which can be found in pdf format on the ABRF web site. In addition to the oral presentation of the results by Peter Hunziker during the AAARG presentation, David Chin, a member of the group, presented the role and the specification of amino acid analysis in an industrial environment. The presentations were very well attended with more than 50 attendees, indicating a lively interest in our study.

The results of the AAA2001 and AAA2003 study will be summarized in an upcoming publication. A new study is in its planning phase and the discussion on which topic a new study should cover is still on-going.

Mike Alterman is stepping down from AAARG after four years. We would like to thank him for his valuable contributions during these years. We also would like to acknowledge Len Packman and Laurey Steinke, our former EB liaisons, for their great help and advice. Current members of the AAARG are David Chin, Reed Harris, Peter Hunziker, Bill Lane (EB liaison), Anthony Le, Susana Linskens, and Johann Schaller. For the continuous rotation of group members we are looking for one or two new members to join the group by Spring 2004 or earlier. If you are interested in amino acid analysis and in actively participating in the the ABRF please contact Peter Hunziker (hc.hzinu.ssecca@rekiznuH.reteP).

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