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J Biomol Tech. 2003 June; 14(2): 158.
PMCID: PMC2279904


The Nucleic Acids Research Group (NARG) has evolved over the years to keep pace with the rapidly changing technology in the Nucleic Acids field. Past endeavors have focused on nucleic acids synthesis chemistry, then DNA sequencing, and back to a focus on DNA synthesis when the DNA Sequence Research Group evolved. In recent years, NARG has encouraged interactions among all ABRF members who are interested in nucleic acids synthesis chemistry and the methods of quality analysis. A major emphasis was on understanding how different options available for the preparation of these reagents may affect their performance in different nucleic acid based technologies. The past two years have seen the NARG focus on the preparation of dual labeled FRET probes for use in Real-Time PCR. These studies have demonstrated that these probes are easy to make and that even crude probes can work well in Real-Time PCR. Any DNA synthesis laboratory is capable of making these probes. For the next few years, the NARG plans to focus their attention on other aspects of Real-Time PCR. The NARG plans to survey ABRF laboratories about their Real-Time PCR facilities and implement studies on primer design or multiplexing or the accuracy of SYBR® Green vs Taqman® assays. Please send us your suggestions!

The NARG is saddened to lose the expertise of Kathy Mills and Greg Buck and we wish them well in their future endeavors. We are currently seeking new members who have experience in the Real-Time PCR area. Please contact us by looking at the NARG webpage at

Articles from Journal of Biomolecular Techniques : JBT are provided here courtesy of The Association of Biomolecular Resource Facilities