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The Fragment Analysis Research Group (FARG) completed the 2003 study, “Current Trends in Methodology and Instrumentation Used for DNA Fragment Analysis: Results from an Online Survey,” and presented results at the annual conference of the Association of Biomolecular Resource Facilities (ABRF) in Denver, Colorado this year. The study was an online survey of ABRF members and other facilities that focus on methods of fragment analysis in a resource laboratory. There was a strong participation this year. Participants were asked questions that related to the current applications and instrumentation used in conducting a fragment analysis service and then compared with a similar study performed by FARG 4 years ago. Results from the study were presented in a poster now available through the ABRF web site at http://www.abrf.org/index.cfm/group.show/FragmentAnalysis.40.htm#R_2. The study was also described in an oral presentation during the annual Research Group report conducted during the ABRF conference. Because of the level of participation during the ABRF conference, the Research Group report period included a short round table session. Those ABRF members that attended the Group report brought questions and concerns to an open forum where discussion was encouraged.
FARG also presented a tutorial session to cover methods that are used to determine single nucleotide polymorphisms (SNPs) in a DNA template. The first speaker gave a detailed discussion focusing on the single base extension method and the flexibility of the method across several platforms, including automated sequencers, HPLC, chip arrays, and MALDI-MS. The second speaker presented materials concerning one specific platform associated with automated sequencers and covered more detail into specific applications and troubleshooting. Both the tutorial and the research group presentation were well attended.
The future plans for FARG are to upload the study presentation and tutorial to the ABRF web site, to be completed in May 2003. The results from the FARG study 2003 will be written in manuscript form and submitted to the Journal of Biomolecular Techniques.
FARG would like to express its appreciation to the participants of the FARG study 2003. We would also like to welcome our newest member, Joe Forrester from the University of Missouri.