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J Biomol Tech. 2003 March; 14(1): 46.
PMCID: PMC2279896


Results from the Peptide Synthesis Research Group (PSRG) 2001 study “Synthesis and Purification of a Difficult Peptide Sequence” revealed a large disparity for the homogeneity of products provided to clients as purified peptides. It was no surprise that the 2001 study demonstrated that preparation of a synthetically challenging peptide resulted in a variation of product purity. What was of interest was the large range of peptide homogeneity that core facilities deemed as pure. Hence, the PSRG is in the midst of designing a follow-up study to evaluate the level of homogeneity achieved from the purification of an inherently difficult synthetic peptide, and to compare those results with the methodologies employed. Thus, in order to provide the membership with suggestions to enhance their ability to increase the quality of synthetically difficult peptides and establish a minimum level of product purity, it is necessary to better understand the variety of purification processes and resulting product yield and homogeneity. The design strategy and methodology for obtaining the necessary information will be provided as a prelude to elicit an even larger participation from the ABRF membership in the PSRG 2004 study, “Strategies for the Purification of a Difficult Peptide Sequence: A Sequel Generated from Lessons Learned by the 2001 Peptide Synthesis Research Group Study.”

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