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The goal of the Peptide Standards Project is the production, characterization, validation, and establishment of three synthetic peptides as registered and certified peptide reference standards. This project is conducted by the Peptide Standards Project Committee (PSPC) of the ABRF in collaboration with the National Institute of Standards and Technology (NIST). The Peptide Standards Project was originally initiated by joint interactions between the Quality Compliance Committee (QCC) and the Peptide Synthesis Research Group (PSRG). It is the first ABRF project funded by NIST.
The project entails the following: (1) large-scale synthesis of the standard peptides in high (>98%) purity; (2) tests for stability and shelf life of the peptides, (3) packaging of the peptides in small quantities for distribution and analysis by ABRF member laboratories; (4) use of the ABRF member analyses to yield the required Certificate of Analysis as specified by NIST.
The PSPC has successfully accomplished the large-scale synthesis (7.5 mmol) and purification of all three peptides. Conditions for synthesis and purification had been optimized on a smaller scale (0.1 mmol). The scale-up posed challenges both in synthesis and in purification. Reaction monitoring and resin choice proved to be critical for the success of all syntheses, and employment of ion-exchange chromatography in addition to HPLC led to increased yield and purity. Results and conditions of synthesis and purification, quality control data of the purified peptides, as well as a detailed project outline will be presented at the ABRF 2003 meeting.
The peptide sequences are:
Peptide A: DAEPDILELATGYR
Peptide B: KAQYARSVLLEKDAEPDILELATGYR
Peptide C: RQAKVLLYSGR