Search tips
Search criteria 


Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Dev Biol. Author manuscript; available in PMC 2009 January 1.
Published in final edited form as:
PMCID: PMC2238337

Dally Regulates Dpp Morphogen Gradient Formation by Stabilizing Dpp on the Cell Surface


Decapentaplegic (Dpp), a Drosophila homologue of bone morphogenetic proteins, acts as a morphogen to regulate patterning along the anterior-posterior axis of the developing wing. Previous studies showed that Dally, a heparan sulfate proteoglycan, regulates both the distribution of Dpp morphogen and cellular responses to Dpp. However, the molecular mechanism by which Dally affects the Dpp morphogen gradient remains to be elucidated. Here, we characterized activity, stability, and gradient formation of a truncated form of Dpp (DppΔN), which lacks a short domain at the N terminus essential for its interaction with Dally. DppΔN shows the same signaling activity and protein stability as wild-type Dpp in vitro but has a shorter half-life in vivo, suggesting that Dally stabilizes Dpp in the extracellular matrix. Furthermore, genetic interaction experiments revealed that Dally antagonizes the effect of Thickveins (Tkv; a Dpp type I receptor) on Dpp signaling. Given that Tkv can downregulate Dpp signaling by receptor-mediated endocytosis of Dpp, the ability of dally to antagonize tkv suggests that Dally inhibits this process. Based on these observations, we propose a model in which Dally regulates Dpp distribution and signaling by disrupting receptor-mediated internalization and degradation of the Dpp-receptor complex.

Keywords: Dally, heparan sulfate proteoglycan, morphogen, Decapentaplegic, Drosophila


Morphogens are signaling molecules that form a concentration gradient across the developmental field and specify different cell fates by activating different sets of genes in a concentration dependent manner. Decapentaplegic (Dpp), a Drosophila member of bone morphogenetic proteins (BMPs), serves as a morphogen during wing development and orchestrates patterning along the anterior (A)-posterior (P) axis (Lecuit et al., 1996; Nellen et al., 1996; Entchev et al., 2000; Tanimoto et al., 2000; Teleman and Cohen, 2000; Muller et al., 2003). Dpp is expressed in a stripe of cells adjacent to the A/P compartment boundary and spreads toward the distal cells of both A and P sides. Proper formation of the Dpp gradient is critical for precise induction of target genes, such as spalt and optomotor-blind, that are induced at different thresholds of Dpp. However, the molecular mechanism by which the Dpp morphogen gradient is established and maintained is poorly understood.

The balance between production and degradation of morphogen molecules is postulated to critically regulate gradient formation (Wolpert, 1969). Therefore, regulation of morphogen degradation can function as a major step to determine the shape of the gradient. During Drosophila embryogenesis, Wingless (Wg) forms an asymmetric gradient along the A-P axis, which is achieved by asymmetric patterns of Wg protein degradation (Dubois et al., 2001). The BMP gradient also seems to be controlled by morphogen degradation. It has been suggested that extracellular Dpp is internalized by receptor-mediated endocytosis and rapidly turned over in the wing disc (Entchev et al., 2000; Teleman and Cohen, 2000). Enhancement of degradation by expressing a dominant gain-of-function form of small GTPase Rab7 resulted in a compression of the Dpp signaling range in the wing disc (Entchev et al., 2000). Furthermore, recent computational studies suggested the importance of BMP degradation during gradient formation in the wing disc (Lander et al., 2005) and in the embryo (Mizutani et al., 2005).

Several lines of evidence have shown that heparan sulfate proteoglycans (HSPGs) of the glypican family play key roles in regulation of morphogen signaling and distribution. Glypicans are glycosylphosphatidylinositol (GPI)-anchored HSPGs and consist of a protein core to which heparan sulfate (HS) chains are covalently attached. The HS chains have a variety of sulfate modifications and provide binding sites for many growth factors. Dally, one of two Drosophila glypicans, regulates both signaling and distribution of Dpp in the developing wing (Fujise et al., 2003; Belenkaya et al., 2004). In dally mutant wing discs, the Dpp gradient can not be formed properly: Dpp signaling monitored by phosphorylation of Mothers against dpp (Mad) was abnormally reduced in the receiving cells (Fujise et al., 2003). In clones of cells overexpressing Dally, Dpp signaling is elevated in a cell autonomous fashion, indicating that Dally increases the sensitivity of cells to Dpp. Thus Dally serves as a co-receptor for Dpp, but the molecular basis for its control of Dpp signaling and distribution is not yet understood.

In order to understand the roles of HSPGs in morphogen gradient formation, two molecular genetic approaches are useful. First, one can use mutant cells deficient for a HSPG to observe the consequence of losing this molecule. Recent experiments using the genetic mosaic system showed that the levels of Dpp protein were significantly reduced within glypican-deficient clones (Belenkaya et al., 2004). This phenomenon can be explained by either inhibition of Dpp movement (Dpp can not enter the dally mutant cells) or Dpp destabilization (Dpp is rapidly degraded in the dally mutant cells). An alternative and complementary approach is to study the behavior of a mutant morphogen molecule that lacks the ability to interact with HSPGs but retains all other activities of the wild-type form. For example, a truncated form of Xenopus BMP4 was used to study the functions of HSPGs in BMP4 morphogen distribution during embryogenesis (Ohkawara et al., 2002). BMPs possess a stretch of basic amino acid residues in their N-terminal region that is essential for interacting with heparin (Ruppert et al., 1996). The mutant BMP4 molecule lacking this N-terminal segment migrated further than wild-type BMP4 in an embryo, suggesting that HSPGs restrict BMP diffusion.

In this study, we generated a mutant form of Dpp, DppΔN. This truncated form of Dpp lacks a short domain (seven amino acid residues) at the N terminus essential for interacting with heparin and Dally. Our results showed that DppΔN exhibits comparable signaling activity and in vitro protein stability to the wild-type form, but has a shorter half-life in vivo: it is more quickly internalized by cells for degradation than wild-type Dpp and therefore fails to form a normal gradient in tissue. These results strongly suggest that Dally stabilizes Dpp on the cell surface and/or in the extracellular matrix.

Materials and Methods

DNA constructs

HA-dppΔN cDNA was constructed by introducing a small deletion in HA-dpp cDNA (Ross et al., 2001) using the Quickchange Site-Directed Mutagenesis Kit (Stratagene). The two PCR primers used were: 5′-CGGAACAAGCGGCACGCGAACCACGACGACATGGAG-3′ (sense primer) and 5′-CTCCATGTCGTCGTGGTTCGCGTGCCGCTTGTTCCG-3′ (antisense primer). The PCR products were cloned into two plasmid vectors: pUAST (Brand and Perrimon, 1993), a general fly transformation vector for in vivo overexpression experiments, and pBRAcpA (Cherbas et al., 1994), a S2 cell expression vector for overexpressing an inserted gene under the control of the actin 5C promoter.

To construct pCaspeR hs-HA-dpp, HA-dpp cDNA was amplified by PCR using the following primers: 5′-GAAGAAGGAATTCCACTCGACCATAGGAAACTTTATAAGACTG-3′ (sense primer) and 5′-GAAGAAGAGATCTGATTTCCCATCGCATTCACTCGGTTGAGTG-3′ (antisense primer). The PCR product was cloned into EcoRI and BglII sites of pCaspeR hs.

A transgenic RNAi construct for dally, pUAST-IR-dally, was generated as genomic-cDNA fusion construct (Kalidas and Smith, 2002). Genomic and cDNA fragments were amplified by PCR using following primers: 5′-GGGCGGCCGCACGTGCAAACTGCATCTCC-3′ (genomic sense primer), 5′-GGGCATGCGCTGTTGCTCATCGCAGAAG-3′ (genomic antisense primer), 5′-GGGCATGCGGCCCACTTTACGTTGTTC-3′ (cDNA sense primer), and 5′-GGCTCGAGACGTGCAAACTGCATCTCC-3′ (cDNA antisense primer). The genomic fragment ranges from exon 5 to exon 8 of the dally gene (corresponding to the sequence from 1589 to 2087 of dally cDNA) and contains a NotI site at the 5′ end and a SphI site at the 3′ end. The cDNA fragment bearing the sequence from 1589 to 1998, which includes no splice donor sequence when inverted, contains a XhoI site at the 5′ end and a SphI site at the 3′ end. Each PCR product was cloned into pBluescript. The genomic fragment digested with NotI and SphI was ligated to the inverted cDNA fragment digested with XhoI and SphI, and subcloned into pUAST vector.


Drosophila S2 tissue culture cells were transfected with pActin-GAL4, pUAST-Myc-dally, and pBRAcpA-HA-dpp or pBRAcpA-HA-dppΔN. After incubation at 25°C for 72 hours, the cell cultures were solubilized using lysis buffer (phosphate buffered saline [PBS] containing 2% Triton X-100, pH 7.0). The soluble fractions were incubated with protein G sepharose conjugated with rat anti-HA antibody (3F10, Roche) for 16 hours at 4°C with agitation. After incubation, precipitates were washed with wash buffer (50 mM Tris-HCl, 150 mM NaCl, and 0.2% Triton X-100, pH 7.4), and proteins bound to anti-HA antibody were eluted with SDS sample buffer. The eluted proteins were subjected to western blot analysis using mouse anti-Myc (9E10) (1:2,000, Sigma) and mouse anti-HA antibodies (12CA5) (1:5,000, Roche).

Heparin-binding assay

S2 cells were transfected with pBRAcpA-HA-dpp or pBRAcpA-HA-dppΔN constructs. After incubation at 25°C for 72 hours, the medium fractions from these cultures were collected and applied to a HiTrap Heparin column (GE Healthcare). The column was washed with 10 bed volume of PBS, and proteins retained on the column were eluted with elution buffer (1 M NaCl/PBS). The eluted protein samples were analyzed by western blot analysis using mouse anti-HA antibody (12CA5) (1:5,000, Roche).

Cell-based Dpp signaling assay

Cell-based Dpp signaling assays were carried out according to the protocol described previously (Shimmi et al., 2005). S2 cells were transfected with Flag-tagged Mad cDNA and incubated at 25°C for 72 hours. Separately, conditioned medium was prepared from S2 cells expressing HA-Dpp or HA-DppΔN, and protein levels of Dpp and DppΔN were determined by western blotting. The Mad-expressing S2 cells were incubated with conditioned medium containing an equivalent amount of Dpp or DppΔN protein. After incubation at 25°C for 4 hours, cells were lysed in SDS sample buffer. Dpp signaling was assayed by western blot analysis using rabbit anti-pMad antibody (1:5,000, a gift from T. Tabata and P. ten Dijke; (Tanimoto et al., 2000)). Levels of total Mad protein were examined as controls using mouse anti-Flag antibody (M2) (1:1,000, Sigma).

Cell-based Dpp stability assay

Cell-based protein stability assays were performed as previously described (Bhanot et al., 1996) with some modifications. HA-Dpp and HA-DppΔN were expressed in S2 cells and conditioned media containing these proteins were prepared. After the relative protein levels of HA-Dpp and HA-DppΔN were determined by western blotting, the same amount of the two proteins were incubated at 25°C with separately prepared S2 cell cultures with or without Dally overexpression. After incubation for 30 minutes, the cells were washed with M3 insect medium (Sigma) to remove unbound HA-Dpp or HA-DppΔN. After washing, the cells were resuspended in M3 insect medium at a density of 4 × 106 cells/ml and incubated at 25°C to allow cells to internalize and degrade Dpp or DppΔN. After incubation for the indicated times (0–6 hours), cells were recovered and dissolved in SDS sample buffer. Supernatant fractions were also analyzed to determine whether HA-tagged proteins were released from the cells during incubation. Polyadenylic acid (Sigma) was mixed with supernatants from each time point during the experiment (0–6 hours) to give a final concentration of 10 μg/ml; an equal volume of 20% trichloroacetic acid (TCA) was added, and the samples were stored at −20°C overnight. After centrifugation, precipitates were washed with acetone to remove the residual TCA and suspended in SDS sample buffer. All samples were subjected to western blot analysis using anti-HA (12CA5, 1:5,000) as a primary antibody and IRDye800 conjugated anti-mouse IgG (1:10,000, Rockland Immunochemicals) as a secondary antibody. The relative intensity of signals for HA-Dpp and HA-DppΔN proteins were determined using LI-COR Odyssey Blot system (LI-COR Biosciences).

As a control, we tested protein stability in a cell-free system. HA-Dpp and HA-DppΔN were incubated in M3 medium at 25°C, and HA-tagged proteins were detected as described above.

Acid wash treatment

Acid washing to remove membrane-bound proteins from S2 cells was carried out as previously described (Miller et al., 2000; Liu et al., 2006) with some modifications. S2 cells with or without thickveins (tkv) RNA interference (RNAi) treatment (described below) were allowed to bind HA-Dpp for 30 minutes at room temperature as above. After washing with M3 insect medium, cells were incubated with acid wash buffer (0.2 M acetic acid and 0.5 M NaCl, pH 2.5) for 5 minutes at room temperature to strip membrane-bound proteins off the cell surface. After centrifugation, the cell fraction was suspended in SDS sample buffer, and HA-Dpp was detected by western blotting using anti-HA antibody. RNAi in S2 cells was performed as described previously (Shimmi et al., 2005). S2 cells were transfected with 15 μg of tkv dsRNA and incubated at 25°C for 72 hours to knock-down tkv.

Drosophila strains

Transformants bearing UAS-HA-dppΔN, hs-HA-dpp, or UAS-IR-dally were generated by P element-mediated transformation. UAS-HA-dpp (Ross et al., 2001) and UAS-HA-dppΔN were used to visualize in vivo distribution of Dpp and DppΔN, respectively. Two UAS-HA-dppΔN transgenic strains (#1 and #2) that show different expression levels of HA-DppΔN were used. Western blotting showed that the level of HA-DppΔN protein expressed in dpp>HA-dppΔN (#2) was 5-fold higher than that in dpp>HA-dppΔN (#1) (Fig. S1). UAS-HA-dpp (A12) and UAS-HA-dppΔN (#1) produce indistinguishable levels of HA-tagged proteins when induced by dpp-GAL4 driver. dpp-GAL4, hh-GAL4, and engrailed (en)-GAL4 were used to induce expression of UAS transgenes and are described in Fly Base. Other transgenic lines used were: UAS-dally (Tsuda et al., 1999), UAS-Myc-dally (Takeo et al., 2005), and UAS-tkv (Tanimoto et al., 2000). All flies were maintained at 25°C except where noted.


Conventional immunostaining of HA-tagged proteins in third larval instar wing discs was performed as previously described (Fujise et al., 2001) using rat anti-HA antibody (3F10) (1:200, Roche). The same antibody (3F10) (1:20) was used for extracellular staining (Strigini and Cohen, 2000; Takeo et al., 2005). The Dpp activity gradient was visualized using rabbit anti-pMad antibody (1:1,000; (Tanimoto et al., 2000; Fujise et al., 2003). The primary antibodies were detected by Alexa Flour conjugated anti-rabbit IgG or anti-rat IgG antibodies (1:500, Molecular Probes).

In vivo Dpp stability assays

We used two different assays to monitor Dpp stability in vivo. For the first assay, UAS-HA-dpp and UAS-HA-dppΔN were expressed at the anteroposterior compartment boundary of the wing disc by dpp-GAL4. Two UAS-HA-dppΔN transgenic strains (#1 and #2) that express different levels of HA-DppΔN were used. Wing discs were dissected from late third instar larvae, and HA-Dpp and HA-DppΔN proteins were pulse-labeled extracellulary with rat anti-HA antibody (1:20) for 1 hour. During labeling, wing discs were incubated with the antibody at 4°C to prevent endocytosis (Mathew et al., 2005). After washing with M3 insect medium to remove unbound antibody, the discs were further incubated in medium at 25°C to allow disc cells to internalize and degrade the pulse-labeled protein. After incubation for 3 hours, the discs were fixed and stained with Alexa Flour conjugated secondary antibodies to detect extracellular Dpp and DppΔN. All images for anti-HA staining were taken at identical settings including gain, pinhole size, dwell time, image size, and scan time. The signal intensity in the wing pouch area was measured by NIH image using the ‘Plot Profile’ function. At least 5 wing discs were used to obtain averaged values.

For the second in vivo Dpp stability assay, we generated a transgenic fly strain bearing a HA-tagged dpp transgene downstream of the heat-shock promoter (hs-HA-dpp). We first determined the time course of degradation of Dpp expressed from this transgene. hs-HA-dpp transgenic animals were cultured at 25°C until third instar larval stage, transcription of HA-dpp was induced by a heat-shock treatment (37°C for 1.5 hours), and larvae were allowed to recover at 25°C. Tracing levels of HA-Dpp along the time course revealed that Dpp levels peaked at 2–3 hours after the heat-shock (data not shown). Afterward, HA-Dpp gradually was degraded and the signals were undetectable at 8 hours after the induction.

Based on this information, we analyzed Dpp levels at 4 and 6 hours after the heat-shock treatment in wing discs from hs-HA-dpp/+; hh-GAL4/+, hs-HA-dpp/UAS-dally-Myc; hh-GAL4/+, and hs-HA-dpp/UAS-IR-dally; hh-GAL4/UAS-IR-dally animals. Wing discs were dissected at these times and stained with rat anti-HA antibody (3F10, 1:200) by the conventional staining protocol. Results of Dpp staining at 4 hours after induction are presented in Figure 7, but we obtained similar results at 6 hours.

Figure 7
The effect of Dally on Dpp protein stability in the wing disc


The N-terminal domain of Dpp is essential for binding to Dally

A previous study demonstrated that the N-terminal segment of basic amino acid residues in Xenopus BMP4 is essential for its binding to heparin, and is important for its normal distribution in an embryo (Ohkawara et al., 2002). Drosophila Dpp also has a stretch of basic amino acid residues in the corresponding region (Ruppert et al., 1996; Ohkawara et al., 2002). To investigate the role of HSPGs in regulating Dpp signaling, we generated a truncated form of Dpp (DppΔN) that lacks seven amino acid residues at the N-terminus (Fig. 1A). We transfected Drosophila S2 cells with cDNAs encoding HA-tagged Dpp (HA-Dpp) and DppΔN (HA-DppΔN) and characterized proteins secreted into the conditioned media. HA-Dpp migrates in a SDS-gel as two bands of molecular size 25 K and 23 K (Fig. 1B;(Shimmi et al., 2005)). These two mature forms of Dpp protein are produced by cleavage at different positions of the Dpp precursor by furin-type proprotein convertases (Kunnapuu and Shimmi, unpublished result).

Figure 1
N-terminal basic domain of Dpp is essential for binding with Dally

We first examined the affinities of HA-Dpp and HA-DppΔN for heparin using heparin-affinity column chromatography. As previously shown (Groppe et al., 1998), wild-type Dpp was retained on the heparin column. Interestingly, we found that the two HA-Dpp fragments with different molecular sizes show different heparin-binding properties: the 25 K (upper) band strongly associated with heparin, while the 23 K (lower) band was only partially retained on the column (Fig. 1B). In contrast, DppΔN was found exclusively in the flow-through fraction and showed no affinity for heparin (Fig. 1B), confirming that the N-terminal basic segment of Dpp functions as a heparin-binding domain. The recently mapped cleavage site for the smaller (23 K) Dpp species is within the N-terminal basic region (Kunnapuu and Shimmi, unpublished result); therefore the low affinity of the 23 K fragment for heparin is consistent with partial loss of the heparin-binding domain.

We next examined whether DppΔN can interact with Dally by coimmunoprecipitation experiments. As depicted in Fig. 1C, Myc-Dally co-precipitated with Dpp, indicating that Dally forms a complex with Dpp in vitro. No co-precipitation was detected in the absence of Dpp, indicating that the binding of Dally to Dpp is specific. In contrast, we could not detect binding between Myc-Dally and HA-DppΔN (Fig. 1C). These results indicate that the N-terminal basic region of Dpp is essential for its interaction with Dally.

In vitro characterization of DppΔN

We evaluated other properties of DppΔN, including its secretion and in vitro signaling activity. First, to confirm that DppΔN is secreted normally from a cell, we compared levels of Dpp and DppΔN proteins secreted by S2 cells. Western blot analysis using cell and medium fractions showed that comparable levels of HA-Dpp and HA-DppΔN were detected in the medium fractions (Fig. 2A), indicating that truncation of the N-terminal basic segment does not affect secretion of Dpp.

Figure 2
Secretion and in vitro signaling activity of DppΔN

Second, we examined signaling activity of DppΔN by the cell-based signaling assay (Ross et al., 2001; Shimmi and O’Connor, 2003; Shimmi et al., 2005). Mad-expressing S2 cells were treated with Dpp or DppΔN for 4 hours at 25°C. Stimulation of Dpp signaling was assessed by phosphorylation of Mad. Western blotting using anti-pMad antibody revealed that DppΔN induced the same level of Mad phosphorylation as Dpp (Fig. 2B). This result demonstrated that the N-terminal basic domain is not required for Dpp signaling activity.

Phenotypes induced by overexpression of dpp and dppΔN

We assessed in vivo activities of Dpp and DppΔN by expressing them using the GAL4/UAS system. To allow quantitative comparison of the phenotypes, we used two transgenic lines, UAS-HA-dpp (12A) and UAS-HA-dppΔN (#1), which produce comparable levels of HA-tagged molecules when induced by the same GAL4 driver (Fig. S1). Expression of HA-dpp using dpp-GAL4 at 25°C caused pupal lethality in all animals due to the overdose of dpp (Fig. 3A). In contrast, under the same conditions, 43% of dppΔN-expressing animals survived until the adult stage. When animals were reared at 18°C, lethality induced by expression of dpp and dppΔN was negligible. At this temperature, 66% of the dpp-expressing animals exhibited a wing outgrowth phenotype (Fig. 3B). Expression of DppΔN induced the same phenotype with much lower penetrance: only 10% of the animals expressing dppΔN showed the wing outgrowth (Fig. 3B). Thus, the in vivo activity of DppΔN is significantly weaker than that of wild-type Dpp despite the similar in vitro activities of these molecules.

Figure 3
Comparison of in vivo activities of Dpp and DppΔN

Extracellular distribution of Dpp and DppΔN in the wing disc

Since DppΔN failed to interact with Dally, which affects the shape of the Dpp gradient, we examined gradient formation by DppΔN in vivo. The wing discs from dpp>HA-dpp (12A) and dpp>HA-dppΔN (#1) animals were stained with anti-HA antibody using conventional and extracellular staining protocols. We observed indistinguishable levels and patterns of HA-Dpp and HA-DppΔN by the conventional staining protocol, suggesting that both proteins are synthesized at similar levels (Fig. 4A and B).

Figure 4
Distribution of Dpp and DppΔN in the wing discs

In contrast, we found that the patterns of extracellular Dpp and DppΔN staining were remarkably different. In the extracellular staining method, the primary antibody is incubated with the tissue before fixation (Strigini and Cohen, 2000). Under this condition, the antibody is only able to access extracellular antigens. Using this protocol, we observed that wild-type Dpp is widely spread and forms a long-range gradient along the entire length of the anterior-posterior axis of the wing disc (Fig. 4C; (Belenkaya et al., 2004)). We found that signals for DppΔN in the extracellular space were significantly weaker than those of Dpp (Fig. 4D). Intensity plots of signals for extracellular Dpp and DppΔN revealed a shallow gradient of DppΔN (Fig. 4E). Thus, DppΔN failed to form a normal concentration gradient and its level in the extracellular space was very low despite its normal level of synthesis. In summary, the N-terminal basic domain of Dpp that is required for binding to Dally is essential for Dpp’s normal distribution in a tissue.

Stability of DppΔN protein in vitro

The weak extracellular staining of DppΔN suggested several possible molecular defects of this mutant protein. For example, DppΔN may not be secreted properly into the extracellular space, but we thought that defective secretion was unlikely since we observed that DppΔN is secreted normally in S2 tissue culture cells (Fig. 2A). Alternatively, it may fail to be retained at the cell surface and may be lost by diffusing away or by degradation. To further characterize DppΔN, we analyzed the retention and stability of DppΔN on the cell surface using a cell-based protein stability assay (Bhanot et al., 1996). When HA-Dpp or HA-DppΔN is incubated for up to 6 hours in cell-free medium, no significant degradation is observed (Fig. 5A), indicating that both proteins are stable in the absence of cells. To assess the stability of these proteins on the cell surface, HA-Dpp or HA-DppΔN was incubated for 30 minutes with S2 cells, which express endogenous dally at low levels. After extensive washes, the cells were further incubated for 0-6 hours and the levels of HA-tagged proteins were monitored by western blot analysis (Fig. 5B). We observed that levels of both HA-Dpp and HA-DppΔN gradually declined. There was no significant difference in the rate and patterns of reduction of the two proteins.

Figure 5
Stability of Dpp and DppΔN in S2 cells

We next used S2 cells overexpressing Dally in this assay. We observed a gradual decrease of DppΔN protein during the 6-hour incubation with dally-expressing cells (Fig. 5C), as in S2 cells without dally transfection. Significantly, under the same conditions, we observed much less reduction in HA-Dpp protein levels in this time frame (Fig. 5C), showing that Dpp stably remains in the cell fraction in the presence of high levels of Dally.

Two lines of evidence supported the idea that the reduction in Dpp levels is primarily due to degradation by S2 cells. First, in order to distinguish between an internalized fraction of Dpp and a fraction bound to the cell surface, we performed an acid wash to remove membrane-bound proteins from the cells (Miller et al., 2000; Liu et al., 2006). After incubation of the S2 cells with Dpp for 30 minutes, we treated the cells with acid wash buffer (0.2 M acetic acid and 0.5 M NaCl, pH 2.5) to strip membrane-bound proteins off the cell surface. We found that a majority of Dpp was detected in the cell fraction after the acid wash (Fig. S2). On the other hand, the amount of Dpp in the cell fraction was significantly decreased when receptor-mediated internalization was blocked by tkv RNAi. These results indicated that the major portion of Dpp protein we detect in the protein stability assay has been internalized within 30 minutes incubation by a Tkv-dependent mechanism. This is consistent with previous reports showing that BMPs are quickly internalized by receptor-mediated endocytosis (Jortikka et al., 1997; Miller et al., 2000). Second, to detect Dpp dissociated from the cell surface during the protein stability assay, we collected media used in these assays and precipitated total proteins with TCA. Western blot analysis of the precipitates showed no detectable Dpp proteins in the media (Fig. S3A). Western blotting to determine the detection limit of Dpp in this experiment demonstrated that less than 0.5% of the Dpp initially bound to the cell surface diffused into media during the protein stability assay (Fig. S3B). These observations are consistent with the idea that the reduction in the level of the Dpp proteins represents their degradation by S2 cells. However, we cannot rule out the possibility that a modest amount of Dpp proteins is released into the culture medium.

Stability of DppΔN in the extracellular space of the wing disc

To better understand why the DppΔN gradient in the wing disc differs from that of Dpp, we devised an assay system to directly compare the stability of wild-type Dpp and DppΔN in vivo. Extracellular HA-tagged proteins in dpp>HA-dpp and dpp>HA-dppΔN discs were pulse-labeled with anti-HA antibody and chased for 3 hours at 25°C. After the incubation, the tissues were fixed and the distribution of the HA-tagged protein/antibody complex was monitored.

During incubation, the extracellular gradient of Dpp becomes broader, expanding toward both anterior and posterior edges (Fig. 6A and B). We also noticed an increase of punctate signals, which seem to represent internalized molecules (Fig. 6B). At 3 hours after the pulse-labeling, the total signal intensity of labeled molecules had decreased to 86% of the initial level (Fig. 6C). We found that DppΔN faded away much more quickly than Dpp. The total signal from the dpp>HA-dppΔN (#1) discs was reduced to 39% in 3 hours (Fig. 6D–F). However, since the extracellular levels of HA-DppΔN in dpp>HA-dppΔN (#1) wing discs were significantly lower than the levels of HA-Dpp at time 0 (Fig. 6D, see also Fig. 4D), it was difficult to compare temporal changes in the distributions of these proteins. We therefore used another transgenic line for dppΔN expression, UAS-HA-dppΔN (#2). Western blot analysis showed that this transgenic line produces 5-fold higher levels of DppΔN protein than UAS-HA-dppΔN (#1) when induced by the same GAL4 drivers (Fig. S1). The intensity of extracellular signals in dpp>HA-dppΔN (#2) discs was comparable to that of dpp>HA-dpp (12A) discs at 0 hour incubation (Fig. 6G). However, consistent with results from the UAS-HA-dppΔN (#1) transgene, the level of pulse-labeled DppΔN decreased to 44% in 3 hours (Fig. 6H and I). Thus, deleting the domain required for interaction with Dally resulted in a shorter half-life for detectable Dpp protein in vivo. This result explains why the in vivo activity of DppΔN was significantly weaker than that of Dpp (Fig. 3) even though both proteins showed the same signaling activity in vitro (Fig. 2B).

Figure 6
Stability of Dpp and DppΔN in the developing wing

In order to confirm that lower stability of DppΔN is due to its failure to bind to Dally, we performed this in vivo pulse-chase experiment for Dpp-HA in dally mutant background. Consistent with the results of DppΔN, we observed that signals of Dpp protein declined more rapidly in dally mutant wing discs (Fig. 6M–O) compared to wild-type (Fig. 6A–C). The signal intensity of pulse-labeled Dpp decreased in 3 hours to 67% in dally heterozygous discs (Fig. 6L) and to 44% in homozygous discs (Fig. 6O), showing a dosage-dependent effect of dally on Dpp stability. It is worth noting that the Dpp gradient does not extend normally in dally mutants (Fig. 6M–O), resembling a previously reported pattern of pMad (the activity gradient) in dally mutants (Fujise et al., 2003). The shrinkage of the Dpp gradient observed in dally mutant discs is also similar to the patterns of DppΔN (Fig. 6D–I). These similarities in temporal and spatial patterns of Dpp in dally mutant wing discs and DppΔN showed that the observed abnormalities of DppΔN can indeed be ascribed to its inability to interact with Dally. Altogether, these observations support the idea that Dally stabilizes Dpp in the extracellular space of the wing disc.

Effects of Dally on Dpp stability in the wing disc

To further analyze Dally’s role in Dpp stability, we developed a second in vivo assay system to monitor Dpp degradation. In this method, Dpp was ubiquitously but transiently expressed in the wing disc using the heat-shock promoter. At 2–3 hours after a heat-shock treatment, the staining intensity of HA-Dpp reached a maximum level (data not shown). Dpp thus synthesized was gradually degraded and became hardly detectable around 8 hours after the induction. Fig. 7B shows a wing disc dissected from a hs-HA-dpp animal at 4 hours after the heat-shock treatment and labeled with anti-HA antibody using the conventional staining protocol.

To determine if changes in the dally dosage affect levels of Dpp protein, we reduced dally expression by inducing a dally transgenic RNAi in the posterior compartment using hh-GAL4 and monitored HA-Dpp. We observed a striking reduction of the HA-Dpp levels in the posterior cells at 4 hours after a heat-shock pulse (Fig. 7C). We also assessed Dpp stability in a disc where dally was overexpressed by hh-GAL4. In contrast to the dally RNAi treatment, a high level of Dpp protein remained in the posterior compartment, suggesting that dally overexpression decreased the rate of reduction of Dpp protein (Fig. 7D). Thus, these two assays consistently showed that Dally slows down the rate of disappearance of Dpp and that the Dally dosage critically affects Dpp stability in vivo.

Dally antagonizes Tkv in Dpp gradient formation

Previous studies suggested that receptor-mediated endocytosis plays a major role in Dpp degradation (Entchev et al., 2000; Teleman and Cohen, 2000). Reducing the level of the type I receptor encoded by tkv seems to reduce the Dpp degradation rate; the Dpp activity gradient visualized by pMad-specific antibody extends to more posterior cells (Fig. 8B). If Dally stabilizes Dpp by inhibiting its endocytosis, dally and tkv may have opposite effects on the Dpp gradient. In fact, dally mutant wing discs show a shrunken gradient (Fig. 8C; (Fujise et al., 2003)), which is the opposite phenotype of tkv mutant discs. We also found that the phenotypes of tkv and dally mutants were mutually suppressed, so that the normal gradient was restored in tkv/+; dally/dally discs (Fig. 8D). Thus, the relative levels of Dally and Tkv appear to be important for maintaining the normal gradient, rather than total amount of these molecules.

Figure 8
dally suppresses the effect of tkv on the Dpp gradient formation

We also examined the effects of elevating the levels of Dally and/or Tkv on the pMad pattern. When dally was overexpressed using en-GAL4, pMad levels were increased in the posterior compartment (Fig. 8E). This phenotype can be explained by the increased sensitivity of cells to Dpp signaling due to the increased levels of Dally. In contrast, tkv overexpression limits Dpp movement, restricting pMad staining to several rows of posterior cells neighboring the anteroposterior compartment border (Fig. 8F; (Lecuit and Cohen, 1998; Tanimoto et al., 2000)). Coexpression of dally suppressed the pMad phenotype caused by en>tkv, expanding the high-pMad region to more posterior cells (Fig. 8G). Furthermore, a tkv mutation could also suppress a dally phenotype in the adult wing: tkv heterozygosity completely rescued the defective wing vein V of dally mutants (data not shown). Altogether, these results indicated that Dally co-receptor antagonizes the effect of Tkv receptor on Dpp signaling.


The Dpp pathway is regulated by multiple cell-surface and extracellular factors (O’Connor et al., 2006). In the developing wing, Dally is one of the key molecules that modulate Dpp signaling. It affects the shape of the Dpp ligand gradient (protein distribution) as well as its activity gradient (spatial patterns of signaling activity) ( Fujise et al., 2003; Belenkaya et al., 2004). Dally and Dpp expressed in S2 tissue culture cells are coimmunoprecipitated, suggesting that Dally forms a complex with Dpp (Fig. 1C; (Kirkpatrick et al., 2006)). We also observed that Dally colocalizes with Dpp and Tkv in cells (Akiyama and Takeo, unpublished result). In addition, we previously demonstrated that Dally enhances Dpp signaling in a cell autonomous fashion (Fujise et al., 2003). These findings suggest that Dally acts as a Dpp co-receptor at least in some developmental contexts (Kramer and Yost, 2003). Interestingly, however, in embryos and in imaginal disc cells close to Dpp-expressing cells, Dpp can mediate signaling without Dally, indicating that HS is not absolutely required for all BMP-dependent processes in vivo.

We demonstrated that DppΔN, which does not bind to heparin, failed to interact with Dally. The easiest interpretation of this result is that wild-type Dpp interacts with Dally via its HS chains. However, a recent study using Surface Plasmon Resonance showed that binding of BMP4 to Dally is not fully inhibited by excess HS (Kirkpatrick et al., 2006). Also, a mutant form of Dally, which does not undergo HS modification, is able to significantly rescue dally mutant phenotypes. These findings suggest that the BMP-glypican interaction is not entirely dependent on the HS chains. One possible explanation for the failure of DppΔN to bind to Dally is that Dpp normally binds to Dally through both the HS chains and its protein core, and DppΔN has reduced affinities for both sites.

Although DppΔN lacks the ability to interact with Dally, it shows normal in vitro protein stability and signaling activity. Therefore, this truncated form of Dpp provided a powerful system to gain insight into the functions of Dally in distribution and signaling of the Dpp morphogen: we were able to use this molecule to elucidate the consequences of lacking the ability to bind HSPGs. In the wing disc, DppΔN cannot form a normal gradient: only a low level of DppΔN was detected in the Dpp-receiving cells (Fig. 4D). Notably, this pattern of DppΔN resembles the Dpp ligand and activity gradients observed in dally mutant wing discs (Fig. 6J–O, Fig. 8C; (Fujise et al., 2003)). A series of in vitro and in vivo Dpp stability assays suggested that DppΔN forms a shallow gradient because it is remarkably unstable in the matrix, and that the stability of Dpp depends on its interaction with Dally.

Our genetic experiments revealed that Tkv and Dally have opposite effects on Dpp gradient formation during wing development. Dally and Tkv share some common properties as components of the Dpp signaling complex: they both autonomously enhance Dpp signaling, and limit migration of Dpp by binding to Dpp protein (Lecuit and Cohen, 1998; Tanimoto et al., 2000; Fujise et al., 2003). Nevertheless, tkv and dally mutually suppress one another’s pMad gradient phenotypes. Consistent with the genetic interactions observed in dally and tkv mutants, the pMad phenotype produced by overexpression of tkv was significantly restored by coexpression of dally. These observations indicate that dally antagonizes tkv in Dpp signaling. Since it has been proposed that Tkv promotes Dpp degradation by receptor-mediated endocytosis (Entchev et al., 2000; Teleman and Cohen, 2000), dally may stabilize Dpp by inhibiting this process.

Altogether, our studies suggest that Dally serves as a co-receptor for Dpp and regulates its signaling as well as gradient formation by disrupting the degradation of the Dpp-receptor complex. In this model, the Dpp signaling complex with Dally co-receptor would remain longer on the cell surface or in the early endosomes to mediate signaling for a prolonged period of time. In contrast, in the absence of Dally, the complex would be relatively quickly degraded. This possible role of Dally can account for the shrinkage of the Dpp gradient in dally mutant wing discs (Fujise et al., 2003). However, we cannot exclude the possibility that DppΔN is lost from the cell surface by lack of retention and further diffuses away.

A previous kinetic analysis of FGF degradation in cultured mammalian vascular smooth muscle cells also showed that HSPG co-receptors can enhance FGF signaling by stabilizing FGF (Sperinde and Nugent, 2000). In these cells, the intracellular processing of FGF-2 occurred in stages: low molecular weight (LMW) intermediate fragments accumulated at the first step. Blocking HS synthesis by treatment of cells with sodium chlorate substantially reduced the half-life of these LMW intermediates, indicating that HSPGs inhibit a certain step of the intracellular degradation of FGF-2. HSPGs have also been implicated in the endocytosis and degradation of Wg (Marois et al., 2006). Wg protein is endocytosed from both apical and basal surfaces of the wing disc and degraded by cells to down-regulate the levels of Wg protein in the extracellular space. Marois et al. (2006) proposed that Dally-like (Dlp), the second Drosophila glypican, regulates the Wg gradient by stimulating the translocation of Wg protein from both the apical and basal membranes to the lateral side, a less active region of endocytosis, thereby inhibiting degradation of Wg protein.

Interestingly, DppΔN behaved differently in vivo from the previously reported mutant Xenopus BMP4 lacking the heparin-binding site (Ohkawara et al., 2002). Although the action range of BMP4 is restricted to the ventral side during Xenopus embryogenesis, the truncated BMP4 migrated further in the embryo. In addition, heparitinase treatment of embryos also resulted in long-range diffusion of BMP4. These findings led to the conclusion that HSPGs trap BMP4 in the extracellular matrix to restrict its distribution in the Xenopus embryo. This activity of HSPGs seems to be opposite to that of Dally in the Dpp receiving cells of the Drosophila wing, where the major role of Dally is to stabilize Dpp protein. In general, ligands that fail to be retained on the cell surface can have any of the following fates: they may (1) migrate further and act as a ligand somewhere else, (2) be degraded by extracellular proteases, or (3) be internalized by endocytosis and degraded intracellularly. Theoretically, a mixture of all these phenomena can happen at the same time in a given tissue. However, which of these predominates may depend on cellular and extracellular environmental conditions such as concentrations of proteases in the matrix and the rate of endocytosis. Therefore, in the absence of HS, whether a major fraction of ligands is degraded or migrates further can be tissue-dependent, and the effects of HSPGs on BMP gradients will vary depending on the developmental context: a mutant BMP4 molecule moves further in a frog embryo, but DppΔN is degraded in Drosophila wing.

Although the results presented here support a role for HSPGs in Dpp stability, they do not rule out the possible involvement of HSPGs in migration of Dpp protein from cell to cell. The gradient of DppΔN is significantly narrower than that of wild-type Dpp (Figs. 4 and and6),6), raising the argument that HS-binding plays a role also in normal Dpp migration in a tissue. Further studies will be required to determine whether or not HSPGs affect morphogen movement. Our study also provides new insight into functional aspects of Dpp processing. Mature forms of Dpp generated by differential cleavages are likely to show different affinities for proteoglycans in the matrix. Therefore, they may have different half-lives and/or spatial distribution patterns in vivo. The biological significance of the occurrence of differently processed forms remains to be elucidated.

Supplementary Material


Supplemental Figure 1. Expression levels of Dpp proteins in UAS-dpp transgenic strains:

Third instar larvae with the genotypes dpp-GAL4, dpp>HA-dpp (A12), dpp>HA-dppΔN (#1), and dpp>HA-dppΔN (#2) were homogenized in SDS sample buffer. Relative levels of HA-tagged proteins in the extracts were compared by western blot analysis using anti-HA antibody.


Supplemental Figure 2. Acid wash of Dpp bound to the cell surface:

(A) S2 cells were incubated with HA-Dpp for 30 minutes at room temperature with agitation to allow cells to bind HA-Dpp. After washing with M3 insect medium, the cells were incubated with M3 medium (lane 1) or acid wash buffer (0.2 M acetic acid and 0.5 M NaCl, pH 2.5) (lane 3) for 5 minutes. The same treatment was also performed with S2 cells pretreated with tkv RNAi (lanes 2 and 4). After centrifugation, HA-Dpp in the cell fractions was detected by western blotting using anti-HA antibody. (B) Effectiveness of tkv RNAi treatment was examined by RT-PCR. Relative levels of tkv mRNA in total RNA samples isolated from S2 cells without (lane 1) or with (lane 2) the RNAi treatment are shown (top). The levels of RP49 RNA are indicated as an internal control (bottom).


Supplemental Figure 3. Detection of Dpp in the culture medium during in vitro Dpp stability assay:

(A) Total proteins in the media used in the cell-based Dpp stability assay (Fig. 5) were precipitated with TCA. Western blot analysis of the precipitates detected no Dpp proteins in the media. (B) To determine the detection limit of TCA precipitation followed by western blotting, a series of dilutions of the Dpp-containing media used in the stability assay were precipitated and HA-proteins were detected as in A. Dpp in the media diluted at 1:2000 was detectable in this system. Since at least 10–20% of total input of Dpp proteins binds to the cell surface (data not shown), the amount of HA-tagged molecules released from cell surface during the stability assay was less than 0.5% of the cell-associated fraction.


We are grateful to T. Tabata, P. ten Dijke, M. O’Connor, and the Bloomington Stock Center for fly stocks and reagents. We thank C. Kirkpatrick for critical reading of the manuscript and helpful comments. This work was supported by research grants from NIH and Human Frontier Science Program.


Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.


  • Belenkaya TY, Han C, Yan D, Opoka RJ, Khodoun M, Liu H, Lin X. Drosophila Dpp morphogen movement is independent of dynamin-mediated endocytosis but regulated by the glypican members of heparan sulfate proteoglycans. Cell. 2004;119:231–44. [PubMed]
  • Bhanot P, Brink M, Samos CH, Hsieh JC, Wang Y, Macke JP, Andrew D, Nathans J, Nusse R. A new member of the frizzled family from Drosophila functions as a Wingless receptor. Nature. 1996;382:225–30. [PubMed]
  • Brand AH, Perrimon N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 1993;118:401–15. [PubMed]
  • Cherbas L, Moss R, Cherbas P. Transformation techniques for Drosophila cell lines. Methods Cell Biol. 1994;44:161–79. [PubMed]
  • Dubois L, Lecourtois M, Alexandre C, Hirst E, Vincent JP. Regulated endocytic routing modulates wingless signaling in Drosophila embryos. Cell. 2001;105:613–24. [PubMed]
  • Entchev EV, Schwabedissen A, Gonzalez-Gaitan M. Gradient formation of the TGF-beta homolog Dpp. Cell. 2000;103:981–91. [PubMed]
  • Fujise M, Izumi S, Selleck SB, Nakato H. Regulation of dally, an integral membrane proteoglycan, and its function during adult sensory organ formation of Drosophila. Dev Biol. 2001;235:433–48. [PubMed]
  • Fujise M, Takeo S, Kamimura K, Matsuo T, Aigaki T, Izumi S, Nakato H. Dally regulates Dpp morphogen gradient formation in the Drosophila wing. Development. 2003;130:1515–22. [PubMed]
  • Groppe J, Rumpel K, Economides AN, Stahl N, Sebald W, Affolter M. Biochemical and biophysical characterization of refolded Drosophila DPP, a homolog of bone morphogenetic proteins 2 and 4. J Biol Chem. 1998;273:29052–65. [PubMed]
  • Jortikka L, Laitinen M, Lindholm TS, Marttinen A. Internalization and intracellular processing of bone morphogenetic protein (BMP) in rat skeletal muscle myoblasts (L6) Cell Signal. 1997;9:47–51. [PubMed]
  • Kalidas S, Smith DP. Novel genomic cDNA hybrids produce effective RNA interference in adult Drosophila. Neuron. 2002;33:177–84. [PubMed]
  • Kirkpatrick CA, Knox SM, Staatz WD, Fox B, Lercher DM, Selleck SB. The function of a Drosophila glypican does not depend entirely on heparan sulfate modification. Dev Biol. 2006;300:570–82. [PubMed]
  • Kramer KL, Yost HJ. Heparan sulfate core proteins in cell-cell signaling. Annu Rev Genet. 2003;37:461–84. [PubMed]
  • Lander AD, Nie Q, Wan FY. Spatially distributed morphogen synthesis and morphogen gradient formation. Math Biosci Eng. 2005;2:239–62. [PubMed]
  • Lecuit T, Brook WJ, Ng M, Calleja M, Sun H, Cohen SM. Two distinct mechanisms for long-range patterning by Decapentaplegic in the Drosophila wing. Nature. 1996;381:387–93. [PubMed]
  • Lecuit T, Cohen SM. Dpp receptor levels contribute to shaping the Dpp morphogen gradient in the Drosophila wing imaginal disc. Development. 1998;125:4901–7. [PubMed]
  • Liu ZH, Tsuchida K, Matsuzaki T, Bao YL, Kurisaki A, Sugino H. Characterization of isoforms of activin receptor-interacting protein 2 that augment activin signaling. J Endocrinol. 2006;189:409–21. [PubMed]
  • Marois E, Mahmoud A, Eaton S. The endocytic pathway and formation of the Wingless morphogen gradient. Development. 2006;133:307–17. [PubMed]
  • Mathew D, Ataman B, Chen J, Zhang Y, Cumberledge S, Budnik V. Wingless signaling at synapses is through cleavage and nuclear import of receptor DFrizzled2. Science. 2005;310:1344–7. [PMC free article] [PubMed]
  • Miller AF, Harvey SA, Thies RS, Olson MS. Bone morphogenetic protein-9. An autocrine/paracrine cytokine in the liver. J Biol Chem. 2000;275:17937–45. [PubMed]
  • Mizutani CM, Nie Q, Wan FY, Zhang YT, Vilmos P, Sousa-Neves R, Bier E, Marsh JL, Lander AD. Formation of the BMP activity gradient in the Drosophila embryo. Dev Cell. 2005;8:915–24. [PMC free article] [PubMed]
  • Muller B, Hartmann B, Pyrowolakis G, Affolter M, Basler K. Conversion of an extracellular Dpp/BMP morphogen gradient into an inverse transcriptional gradient. Cell. 2003;113:221–33. [PubMed]
  • Nellen D, Burke R, Struhl G, Basler K. Direct and long-range action of a DPP morphogen gradient. Cell. 1996;85:357–68. [PubMed]
  • O’Connor MB, Umulis D, Othmer HG, Blair SS. Shaping BMP morphogen gradients in the Drosophila embryo and pupal wing. Development. 2006;133:183–93. [PubMed]
  • Ohkawara B, Iemura S, ten Dijke P, Ueno N. Action range of BMP is defined by its N-terminal basic amino acid core. Curr Biol. 2002;12:205–9. [PubMed]
  • Ross JJ, Shimmi O, Vilmos P, Petryk A, Kim H, Gaudenz K, Hermanson S, Ekker SC, O’Connor MB, Marsh JL. Twisted gastrulation is a conserved extracellular BMP antagonist. Nature. 2001;410:479–83. [PubMed]
  • Ruppert R, Hoffmann E, Sebald W. Human bone morphogenetic protein 2 contains a heparin-binding site which modifies its biological activity. Eur J Biochem. 1996;237:295–302. [PubMed]
  • Shimmi O, O’Connor MB. Physical properties of Tld, Sog, Tsg and Dpp protein interactions are predicted to help create a sharp boundary in Bmp signals during dorsoventral patterning of the Drosophila embryo. Development. 2003;130:4673–82. [PubMed]
  • Shimmi O, Umulis D, Othmer H, O’Connor MB. Facilitated transport of a Dpp/Scw heterodimer by Sog/Tsg leads to robust patterning of the Drosophila blastoderm embryo. Cell. 2005;120:873–86. [PubMed]
  • Sperinde GV, Nugent MA. Mechanisms of fibroblast growth factor 2 intracellular processing: a kinetic analysis of the role of heparan sulfate proteoglycans. Biochemistry. 2000;39:3788–96. [PubMed]
  • Strigini M, Cohen SM. Wingless gradient formation in the Drosophila wing. Curr Biol. 2000;10:293–300. [PubMed]
  • Takeo S, Akiyama T, Firkus C, Aigaki T, Nakato H. Expression of a secreted form of Dally, a Drosophila glypican, induces overgrowth phenotype by affecting action range of Hedgehog. Dev Biol. 2005;284:204–18. [PubMed]
  • Tanimoto H, Itoh S, ten Dijke P, Tabata T. Hedgehog creates a gradient of DPP activity in Drosophila wing imaginal discs. Mol Cell. 2000;5:59–71. [PubMed]
  • Teleman AA, Cohen SM. Dpp gradient formation in the Drosophila wing imaginal disc. Cell. 2000;103:971–80. [PubMed]
  • Tsuda M, Kamimura K, Nakato H, Archer M, Staatz W, Fox B, Humphrey M, Olson S, Futch T, Kaluza V, Siegfried E, Stam L, Selleck SB. The cell-surface proteoglycan Dally regulates Wingless signalling in Drosophila. Nature. 1999;400:276–80. [PubMed]
  • Wolpert L. Positional information and the spatial pattern of cellular differentiation. J Theor Biol. 1969;25:1–47. [PubMed]