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Mol Med. 1996 January; 2(1): 97–108.
PMCID: PMC2230030

Analysis of the structure and expression of the augmenter of liver regeneration (ALR) gene.

Abstract

BACKGROUND: The gene encoding the hepatotrophic factor Augmenter of Liver Regeneration (ALR) has recently been cloned in the rat. The availability of the mouse form of ALR would allow the analysis of the role of this factor in the physiology of liver and other organs, while the identification of the human homolog would allow the transfer of the great wealth of information that has been generated in animal models to clinically oriented pilot trials, and eventually the therapeutic application of this information. MATERIALS AND METHODS: Standard molecular biology approaches have been used to determine the genomic structure of the ALR gene in the mouse, and to characterize the ALR transcript and its protein product. The human ALR cDNA was also isolated and the amino acid sequence of the human gene product deduced. The mapping of mouse and human ALR genes on mouse and human chromosomes was then completed. RESULTS: The protein coding portion of the mouse ALR gene is comprised of three exons, the first containing the 5' untranslated sequence and the initial 18 bases after the ATG translation initiation codon, the second exon encompasses 198 bases, and the third exon contains the remaining portion of the protein coding sequence. Rat, mouse, and human ALR genes (and protein products) were found to be highly conserved and preferentially expressed in the testis and in the liver. The ALR gene maps to the mouse chromosome 17, in a region syntenic with human chromosome 16, where the T/t region has also been mapped. CONCLUSIONS: ALR appears to be a protein with important physiologic properties, not exclusively limited to liver regeneration, with roles that are involved in the synthesis or stability of the nuclear and mitochondrial transcripts that are present in actively regenerating cells, particularly the germ cells of the testes.

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Selected References

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  • Francavilla A, Hagiya M, Porter KA, Polimeno L, Ihara I, Starzl TE. Augmenter of liver regeneration: its place in the universe of hepatic growth factors. Hepatology. 1994 Sep;20(3):747–757. [PubMed]
  • LaBrecque DR, Pesch LA. Preparation and partial characterization of hepatic regenerative stimulator substance (SS) from rat liver. J Physiol. 1975 Jun;248(2):273–284. [PubMed]
  • Terblanche J, Porter KA, Starzl TE, Moore J, Patzelt L, Hayashida N. Stimulation of hepatic regeneration after partial hepatectomy by infusion of a cytosol extract from regenerating dog liver. Surg Gynecol Obstet. 1980 Oct;151(4):538–544. [PMC free article] [PubMed]
  • TEIR H, RAVANTI K. Mitotic activity and growth factors in the liver of the white rat. Exp Cell Res. 1953 Dec;5(2):500–507. [PubMed]
  • LaBrecque DR, Pesch LA. Preparation and partial characterization of hepatic regenerative stimulator substance (SS) from rat liver. J Physiol. 1975 Jun;248(2):273–284. [PubMed]
  • Francavilla A, Ove P, Polimeno L, Coetzee M, Makowka L, Rose J, Van Thiel DH, Starzl TE. Extraction and partial purification of a hepatic stimulatory substance in rats, mice, and dogs. Cancer Res. 1987 Nov 1;47(21):5600–5605. [PubMed]
  • Hagiya M, Francavilla A, Polimeno L, Ihara I, Sakai H, Seki T, Shimonishi M, Porter KA, Starzl TE. Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene: expression of biologically active recombinant ALR and demonstration of tissue distribution. Proc Natl Acad Sci U S A. 1994 Aug 16;91(17):8142–8146. [PubMed]
  • Starzl TE, Jones AF, Terblanche J, Usui S, Porter KA, Mazzoni G. Growth-stimulating factor in regenerating canine liver. Lancet. 1979 Jan 20;1(8108):127–130. [PubMed]
  • Starzl TE, Watanabe K, Porter KA, Putnam CW. Effects of insulin, glucagon, and insuling/glucagon infusions on liver morphology and cell division after complete portacaval shunt in dogs. Lancet. 1976 Apr 17;1(7964):821–825. [PubMed]
  • Mazzaferro V, Porter KA, Scotti-Foglieni CL, Venkataramanan R, Makowka L, Rossaro L, Francavilla A, Todo S, Van Thiel DH, Starzl TE. The hepatotropic influence of cyclosporine. Surgery. 1990 May;107(5):533–539. [PubMed]
  • Starzl TE, Porter KA, Mazzaferro V, Todo S, Fung J, Francavilla A. Hepatotrophic effects of FK506 in dogs. Transplantation. 1991 Jan;51(1):67–70. [PubMed]
  • Starzl TE, Schreiber SL, Albers MW, Porter KA, Foglieni CS, Francavilla A. Hepatotrophic properties in dogs of human FKBP, the binding protein for FK506 and rapamycin. Transplantation. 1991 Oct;52(4):751–753. [PubMed]
  • Silver LM. The peculiar journey of a selfish chromosome: mouse t haplotypes and meiotic drive. Trends Genet. 1993 Jul;9(7):250–254. [PubMed]
  • Giorda R, Weisberg EP, Ip TK, Trucco M. Genomic structure and strain-specific expression of the natural killer cell receptor NKR-P1. J Immunol. 1992 Sep 15;149(6):1957–1963. [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Dignam JD. Preparation of extracts from higher eukaryotes. Methods Enzymol. 1990;182:194–203. [PubMed]
  • Moshier JA, Deutch AH, Huang RC. Structure and in vitro transcription of a mouse B1 cluster containing a unique B1 dimer. Gene. 1987;58(1):19–27. [PubMed]
  • Copeland NG, Gilbert DJ, Jenkins NA, Nadeau JH, Eppig JT, Maltais LJ, Miller JC, Dietrich WF, Steen RG, Lincoln SE, et al. Genome maps IV 1993. Wall chart. Science. 1993 Oct 1;262(5130):67–82. [PubMed]
  • Hagiya M, Francavilla A, Polimeno L, Ihara I, Sakai H, Seki T, Shimonishi M, Porter KA, Starzl TE. Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene: expression of biologically active recombinant ALR and demonstration of tissue distribution. Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):3076–3076. [PubMed]
  • Nietfeld W, el-Baradi T, Mentzel H, Pieler T, Köster M, Pöting A, Knöchel W. Second-order repeats in Xenopus laevis finger proteins. J Mol Biol. 1989 Aug 20;208(4):639–659. [PubMed]
  • Passananti C, Felsani A, Caruso M, Amati P. Mouse genes coding for "zinc-finger"-containing proteins: characterization and expression in differentiated cells. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9417–9421. [PubMed]
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Schmidt C, Bladt F, Goedecke S, Brinkmann V, Zschiesche W, Sharpe M, Gherardi E, Birchmeier C. Scatter factor/hepatocyte growth factor is essential for liver development. Nature. 1995 Feb 23;373(6516):699–702. [PubMed]
  • Uehara Y, Minowa O, Mori C, Shiota K, Kuno J, Noda T, Kitamura N. Placental defect and embryonic lethality in mice lacking hepatocyte growth factor/scatter factor. Nature. 1995 Feb 23;373(6516):702–705. [PubMed]
  • Sonnenberg E, Meyer D, Weidner KM, Birchmeier C. Scatter factor/hepatocyte growth factor and its receptor, the c-met tyrosine kinase, can mediate a signal exchange between mesenchyme and epithelia during mouse development. J Cell Biol. 1993 Oct;123(1):223–235. [PMC free article] [PubMed]
  • Pause A, Belsham GJ, Gingras AC, Donzé O, Lin TA, Lawrence JC, Jr, Sonenberg N. Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function. Nature. 1994 Oct 27;371(6500):762–767. [PubMed]

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