Search tips
Search criteria 


Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. 1982 September; 151(3): 1146–1152.
PMCID: PMC220390

Genetic analysis of the pyruvate decarboxylase reaction in yeast glycolysis.


Six different pyruvate decarboxylase mutants of Saccharomyces cerevisiae were isolated. They belong to two unlinked complementation groups. Evidence is presented that one group is affected in a structural gene. The fact that five of the six mutants had residual pyruvate decarboxylase activity provided the opportunity for an intensive physiological characterization. It was shown that the loss of enzyme activity in vitro is reflected in a lower fermentation rate, an increased pyruvate secretion, and slower growth on a 2% glucose medium. The different effects of antimycin A on leaky mutants grown on ethanol versus the same mutants grown on glucose support the view that glucose induces some of the glycolytic enzymes, especially pyruvate decarboxylase.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bañuelos M, Gancedo C, Gancedo JM. Activation by phosphate of yeast phosphofructokinase. J Biol Chem. 1977 Sep 25;252(18):6394–6398. [PubMed]
  • Boiteux A, Hess B. Allosteric properties of yeast pyruvate decarboxylase. FEBS Lett. 1970 Aug 31;9(5):293–296. [PubMed]
  • Ciriacy M. Isolation and characterization of yeast mutants defective in intermediary carbon metabolism and in carbon catabolite derepression. Mol Gen Genet. 1977 Jul 20;154(2):213–220. [PubMed]
  • Ciriacy M, Breitenbach I. Physiological effects of seven different blocks in glycolysis in Saccharomyces cerevisiae. J Bacteriol. 1979 Jul;139(1):152–160. [PMC free article] [PubMed]
  • Clifton D, Weinstock SB, Fraenkel DG. Glycolysis mutants in Saccharomyces cerevisiae. Genetics. 1978 Jan;88(1):1–11. [PubMed]
  • Entian KD, Zimmermann FK. Glycolytic enzymes and intermediates in carbon catabolite repression mutants of Saccharomyces cerevisiae. Mol Gen Genet. 1980 Jan;177(2):345–350. [PubMed]
  • Gounaris AD, Turkenkopf I, Buckwald S, Young A. Pyruvate decarboxylase. I. Protein dissociation into subunits under conditions in which thiamine pyrophosphate is released. J Biol Chem. 1971 Mar 10;246(5):1302–1309. [PubMed]
  • Gounaris AD, Turkenkopf I, Civerchia LL, Greenlie J. Pyruvate decarboxylase III. Specificity restrictions for thiamine pyrophosphate in the protein association step, sub-unit structure. Biochim Biophys Acta. 1975 Oct 20;405(2):492–499. [PubMed]
  • HOLZER H. Regulation of carbohydrate metabolism by enzyme competition. Cold Spring Harb Symp Quant Biol. 1961;26:277–288. [PubMed]
  • Hopmann RF. Hydroxyl-ion-induced subunit dissociation of east cytoplasmic pyruvate decarboxylase. A circular dichroism study. Eur J Biochem. 1980 Sep;110(1):311–318. [PubMed]
  • Lam KB, Marmur J. Isolation and characterization of Saccharomyces cerevisiae glycolytic pathway mutants. J Bacteriol. 1977 May;130(2):746–749. [PMC free article] [PubMed]
  • Lancashire WE, Payton MA, Webber MJ, Hartley BS. Petite-negative mutants of Saccharomyces cerevisiae. Mol Gen Genet. 1981;181(3):409–410. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Maitra PK, Lobo Z. A kinetic study of glycolytic enzyme synthesis in yeast. J Biol Chem. 1971 Jan 25;246(2):475–488. [PubMed]
  • Maitra PK, Lobo Z. Yeast pyruvate kinase: a mutant from catalytically insensitive to fructose 1,6-bisphosphate. Eur J Biochem. 1977 Sep;78(2):353–360. [PubMed]
  • Maitra PK, Lobo Z. Genetic studies with a phosphoglucose isomerase mutant of Saccharomyces cerevisiae. Mol Gen Genet. 1977 Nov 4;156(1):55–60. [PubMed]
  • Mitchell P. Protonmotive redox mechanism of the cytochrome b-c1 complex in the respiratory chain: protonmotive ubiquinone cycle. FEBS Lett. 1975 Aug 1;56(1):1–6. [PubMed]
  • Mortimer RK, Schild D. Genetic map of Saccharomyces cerevisiae. Microbiol Rev. 1980 Dec;44(4):519–571. [PMC free article] [PubMed]
  • Schamhart DH, Ten Berge AM, Van De Poll KW. Isolation of a catabolite repression mutant of yeast as a revertant of a strain that is maltose negative in the respiratory-deficient state. J Bacteriol. 1975 Mar;121(3):747–752. [PMC free article] [PubMed]
  • Snow R. An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'. Nature. 1966 Jul 9;211(5045):206–207. [PubMed]
  • Sprague GF., Jr Isolation and characterization of a Saccharomyces cerevisiae mutant deficient in pyruvate kinase activity. J Bacteriol. 1977 Apr;130(1):232–241. [PMC free article] [PubMed]
  • Ullrich J, Kempfle M. Subunit size of cytoplasmic yeast pyruvate decarboxylase. FEBS Lett. 1969 Aug;4(4):273–274. [PubMed]
  • Wills C, Phelps J. A technique for the isolation of yeast alcohol dehydrogenase mutants with altered substrate specificity. Arch Biochem Biophys. 1975 Apr;167(2):627–637. [PubMed]
  • Zimmermann FK, Eaton NR. Genetics of induction and catabolite repression of Maltese synthesis in Saccharomyces cerevisiae. Mol Gen Genet. 1974;134(3):261–272. [PubMed]
  • Zimmermann FK, Kaufmann I, Rasenberger H, Haubetamann P. Genetics of carbon catabolite repression in Saccharomycess cerevisiae: genes involved in the derepression process. Mol Gen Genet. 1977 Feb 28;151(1):95–103. [PubMed]
  • Zimmermann FK, Scheel I. Mutants of Saccharomyces cerevisiae resistant to carbon catabolite repression. Mol Gen Genet. 1977 Jul 7;154(1):75–82. [PubMed]
  • Zimmermann FK, Schmiedt I, ten Berge AM. Dominance and recessiveness at the protein level in mutant x wildtype crosses in Sacchaomyces cerevisiae. Mol Gen Genet. 1969 Aug 15;104(4):321–330. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)