PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Clin Endocrinol Metab. Author manuscript; available in PMC Jan 14, 2008.
Published in final edited form as:
PMCID: PMC2196134
NIHMSID: NIHMS36802
Obesity and Sex Steroid Changes Across Puberty: Evidence for Marked Hyperandrogenemia in Pre- and Early Pubertal Obese Girls*
Christopher R. McCartney,1,2 Susan K. Blank,1,2 Kathleen A. Prendergast,1,2 Sandhya Chhabra,1,2 Christine A. Eagleson,1,2 Kristin D. Helm,1,2 Richard Yoo,3 R. Jeffrey Chang,3 Carol M. Foster,4 Sonia Caprio,5 and John C. Marshall1,2
1 The Center for Research in Reproduction, University of Virginia Health System, Charlottesville, Virginia 22908
2 Division of Endocrinology, Department of Internal Medicine, University of Virginia Health System, Charlottesville, Virginia 22908
3 Division of Reproductive Endocrinology and Infertility, Department of Reproductive Medicine, University of California, San Diego, California 92103
4 Division of Endocrinology, Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT 84108
5 Department of Pediatrics, Yale University School of Medicine, New Haven, CT, United States, 06520
Corresponding author: Christopher R. McCartney, Center for Research in Reproduction, Box 800391, University of Virginia Health System, Charlottesville, Virginia 22908; phone (434) 924-2431; fax (434) 243-6913; e-mail cm2hq/at/virginia.edu
Context
Peripubertal obesity is associated with abnormal sex steroid concentrations, but the timing of onset and degree of these abnormalities remain unclear.
Objective
To assess the degree of hyperandrogenemia across puberty in obese girls, and to assess overnight sex steroid changes in Tanner 1–3 girls.
Design
Cross-sectional analysis.
Setting
General Clinical Research Centers.
Subjects
Thirty normal weight (BMI-for-age < 85%) and 74 obese (BMI-for-age ≥ 95%) peripubertal girls.
Intervention
Blood samples (circa 0500–0700 h) while fasting. Samples from the preceding evening (circa 2300 h) were obtained in 23 Tanner 1–3 girls.
Main outcome measures
Hormone concentrations stratified by Tanner stage.
Results
Compared to normal weight girls, mean free testosterone (T) was elevated 2- to 9-fold across puberty in obese girls, while fasting insulin was 3-fold elevated in obese Tanner 1–3 girls (P < 0.05). Mean LH was lower in obese Tanner 1 and 2 girls (P < 0.05), but not in more mature girls. In a subgroup of normal weight Tanner 1–3 girls (n = 17), mean progesterone (P) and T increased overnight 2.3- and 2.4-fold, respectively (P ≤ 0.001). In obese Tanner 1–3 girls (n = 6), evening P and T were elevated, and both tended to increase overnight (mean 1.4- and 1.6-fold, respectively [P = 0.06]).
Conclusions
Peripubertal obesity is associated with hyperandrogenemia and hyperinsulinemia throughout puberty, being especially marked shortly before and during early puberty. Progesterone and testosterone concentrations in normal weight Tanner 1–3 girls increase overnight, with similar but less evident changes in obese girls.
Keywords: hyperandrogenemia, testosterone, progesterone, estradiol, PCOS, puberty, adolescence, obesity, adiposity, overweight, hyperinsulinemia, insulin, LH
Obesity is associated with hyperandrogenemia (HA) in adult women (1), and a similar relationship has been described in late female adolescence (25). There are few data on the relationship between female obesity and HA in early puberty, a critical time when the hypothalamic-pituitary-ovarian interactions governing reproductive function are established. Recent reports have documented an association between obesity and testosterone (T) in pre- and early pubertal (Tanner breast stages 1–3) girls (6, 7). To delineate the timing and extent of this association across pubertal maturation, we have assessed early morning (fasting) hormone levels in a group of obese and normal weight peripubertal girls stratified by Tanner stage.
In addition to the rise of sex steroid concentrations across female pubertal development, both estradiol (E2) and T exhibit diurnal changes, with nadirs occurring in the late evening hours and peaks between 0600 and 1000 h (811). However, there are no published data regarding diurnal changes in progesterone (P), and it is unknown if obesity modifies the diurnal changes in sex steroids during puberty. Therefore, we evaluated overnight sex steroid changes in a subset of normal weight and obese pre- and early pubertal (Tanner stages 1–3) girls.
Subjects
Our collaborative group collected hormonal and anthropomorphic data in girls across the pubertal spectrum (ages 7–17 y) with varying degrees of adiposity. Building upon previous observations (7), the present analysis is restricted to those girls who are either normal weight (gender-specific body mass index [BMI]-for-age < 85th percentile; n = 30) or obese (BMI-for-age ≥ 95th; n = 74). Girls who were “at risk of overweight” (i.e., BMI-for-age between 85th and 95th percentile) are not included in this analysis since they are generally felt to be at intermediate risk for obesity-related abnormalities. These categories are consistent with those endorsed by the Center for Disease Control and Prevention (CDC) and the American Academy of Pediatrics. Exact BMI-for-age percentiles were calculated using a SAS program that incorporates normative data from the National Health Examination and National Health and Nutrition Examination Surveys (12) (available on the CDC website http://www.cdc.gov/nccdphp/dnpa/growthcharts/sas.htm). Volunteers were recruited through advertisements and from Endocrinology Clinics. Subjects were evaluated with morning fasting blood samples obtained at the University of Virginia Health System (n = 49), the University of Michigan (n = 15), the University of California at San Diego (n = 7), and Yale (n = 33). In older girls where cycle stage was unclear, a morning P concentration > 2 ng/ml was grounds for exclusion. Subjects include 6 and 12 Tanner stage 1; 7 and 23 Tanner 2; 6 and 16 Tanner 3; and 11 and 23 Tanner 4–5 normal weight and obese girls, respectively. Reported racial categories included white (n = 83), black (n = 19), Asian (n = 1), and “other” (n = 1). Seventeen of 104 were of Hispanic descent. Racial and ethnic classifications were balanced between normal weight and obese groups. Some of these subjects were included in a previous report (7).
Study procedures
Study procedures were approved by the Institutional Review Boards at each institution. Informed assent and consent were obtained from study participants and parents, respectively. Participants were taking no medications known to affect the reproductive axis, and none had used hormonal medications for 90 d prior to study.
Each volunteer underwent a detailed history and physical exam including assessment of pubertal stage using the Tanner scale for breast development (13). We did not utilize Tanner scales for hair growth.
An early morning (circa 0500–0700 h) blood sample was obtained for measurement of T, SHBG, LH, FSH, dehydroepiandrosterone sulfate (DHEAS), E2, P, and cortisol. Insulin and glucose were measured on the same blood sample when fasting for at least 6 h was confirmed (n = 80). Twenty-three pre- and early pubertal girls (six Tanner 1, five Tanner 2, and six Tanner 3 normal weight girls; and two obese girls in each of the first three Tanner stages) also had a sample drawn for T, E2, P, and cortisol the preceding evening (circa 2200–2300 h).
Hormonal measurements
Assays were performed by the Ligand Core Laboratory of the Center for Research in Reproduction at the University of Virginia Health System. Total T, E2, and P were measured by radioimmunoassay (Diagnostic Products Corporation, Los Angeles, CA; sensitivities 5.0 ng/dl, 10 pg/ml, and 0.05 ng/ml, respectively; intraassay CVs 4.4–10%, 5.2–6.8%, and 2.9–5.0%, respectively; and interassay CVs 8.8–12.4%, 11.8–15.8%, and 9.1–10.9%, respectively).
Given the possibility of cross-reaction of cortisol in the P assay, we performed a series of studies in which P-free sera with various concentrations of cortisol were assessed using the P assay. Cortisol in concentrations of 2.5 μg/dl and 25 μg/dl measured as approximately 25 and 250 pg/ml in the P assay, respectively, with intermediate cortisol concentrations yielding intermediate P measurements. A cortisol concentration-P measurement nomogram was constructed, which allowed adjustment of measured P concentrations for the contribution of cortisol. Cortisol was measured using radioimmunoassay (Diagnostic Products Corporation, Los Angeles, CA; sensitivity 1 μg/dl; intraassay CV 4.2–4.7%; interassay CV 6.5–8.1%).
SHBG and DHEAS were measured by chemiluminescence (Diagnostic Products Corporation, Los Angeles, CA; sensitivities 0.2 nmol/liter and 7 μg/dl; intrasassay CVs 2.0–3.5% and 5.4–6.2%; and interassay CVs 3.8–5.7% and 6.8–7.8%, respectively). Insulin was measured by chemiluminescence (Diagnostic Products Corporation, Los Angeles, CA; sensitivity 2.6 μIU/ml; intrasassay CV 3.0–4.0%; and interassay CV 8.3–8.8%, respectively), or by radioimmunoassays (Diagnostics Systems Laboratories, Inc., Webster, TX; sensitivity 1.3 μIU/ml; intrasassay CV 7.5–8.5%; and interassay CV 9.5–21%; or Linco Research, Inc., St. Charles, MO; intraassay CV 4.5%, interassay CV 10%). LH and FSH were measured by chemiluminescence (Diagnostic Products Corporation, Los Angeles, CA; sensitivities 0.1 and 0.05 IU/liter; intraassay CVs 2.3–4.1% and 2.2–2.6%; and interassay CVs 5.3–6.6% and 4.9–6.3%, respectively). Glucose was measured via glucose oxidase method using a glucose analyzer (Beckman Instruments, Brea, CA). Samples with measured values below assay sensitivity were assigned the value of the assay's sensitivity.
Free T was calculated from total T and SHBG using the following equation: FT = [T − (N)(FT)]/[(KT)(SHBG)−(KT)(T)+(N)(KT)(FT)]. In this equation, FT = free T (pmol/liter); KT = association constant of SHBG for T (1.0 × 109); T = total T concentration (ng/dl); SHBG = SHBG concentration (nmol/l); and N = (KA)(CA) + 1, where KA = association constant of albumin for T (3.6 × 104), and CA = concentration of albumin (assumed to be 4.3 g/dl) (14).
Homeostatic model assessment (HOMA), an estimate of insulin resistance, was calculated from glucose (expressed as mmol/liter) and insulin (expressed as μIU/ml) using the equation (glucose × insulin) / 22.5 (15).
Statistical Methods
Data are presented as mean ± SEM unless otherwise noted. We employed non-parametric statistical tests, which are based on ranks of observations and require no assumptions about the underlying distribution of data. All hypothesis tests were two-sided.
Wilcoxon rank sum tests (i.e., two-sample Wilcoxon tests) were employed to compare parameters between normal weight and obese girls within their respective Tanner stage grouping. The method of normal approximation was employed for comparisons between normal weight and obese Tanner stage 4–5 girls, since there were at least 10 observations in each group. Exact tests were performed for all other pairwise comparisons, which involved less than 10 observations in the respective normal weight groups. These tests were conducted at the 0.05 level of significance, but we secondarily applied a criterion for significance of 0.005 as guided by the highly conservative Bonferroni method of adjusting for multiple comparisons.
Wilcoxon sign rank tests were used to assess overnight changes in sex steroid values in the group of normal weight and the group of obese Tanner 1–3 girls. Wilcoxon rank sum tests (i.e., two-sample Wilcoxon tests) were employed to evaluate for differences in sex steroid concentrations at 2300 h and 0600 h, respectively, between normal weight and obese Tanner 1–3 girls. These tests were conducted at the 0.05 level of significance with no correction for multiple comparisons.
Early morning hormonal values stratified by Tanner stage
The obese girls in our cohort demonstrated significant hyperandrogenism throughout puberty, and this was especially marked in pre- and early pubertal girls (Table 1 and Figure 1). Compared to normal weight girls, mean total T was 4.5-fold (median 7.1-fold) higher in obese Tanner 1 (prepubertal) girls, and 1.6- and 3.3-fold (median 3.2- and 2.8-fold) elevated in obese Tanner stage 2 and 3 girls, respectively (P < 0.005 for Tanner 1 and 3; P = 0.08 for Tanner 2). Additionally, mean SHBG was 59–69% (median 57–72%) lower in the obese Tanner 1, 2, and 3 girls (P < 0.0001 for Tanner 1; P < 0.001 for Tanner 2 and 3). Together these account for the estimated 8.8-, 2.2-, and 5.8-fold higher mean free T (17.5-, 5.0-, and 5.3-fold higher median free T) in obese Tanner stages 1, 2, and 3 girls, respectively (P < 0.001 for Tanner 1 and 3; P < 0.05 for Tanner 2). Similar differences were observed in the more mature, Tanner 4–5 girls.
Table 1
Table 1
Early morning hormonal data in normal weight and obese peripubertal girls stratified by Tanner stage.
Figure 1
Figure 1
Graphical representation of observed differences between obese and normal weight girls grouped by Tanner stage
To define Tanner stage-specific normal ranges for free T, we evaluated this parameter in normal weight girls with no evidence of hirsutism (and no irregular periods in girls at least 2 y postmenarche). The maximum free T was 4.8 pmol/liter in Tanner 1, 11.0 pmol/liter in Tanner 2, 15.1 pmol/liter in Tanner 3, and 31.2 pmol/liter in Tanner 4–5 girls. The proportion of obese girls with free T values above the Tanner stage-specific normal range was between 60 and 94% (Figure 2).
Figure 2
Figure 2
Proportion of normal weight and obese girls with hyperandrogenemia
Mean fasting insulin was elevated in obese girls across pubertal development, especially among the pre- and early pubertal (stages 1–3) obese girls, whose mean insulin was 2.8- to 7-fold (median 2.4- to 6.4-fold) higher than their normal weight counterparts (P < 0.005 for Tanner 1 and 3; P = 0.055 for Tanner 2). Similarly, compared to their normal weight counterparts, mean HOMA was higher, being increased 7-fold in Tanner 1 (P < 0.005), 2.5-fold in Tanner 2 (P = 0.08), 3.7-fold in Tanner 3 (P < 0.005), and 1.7-fold in Tanner 4–5 (P < 0.005) obese girls.
Mean LH concentrations were lower in obese Tanner stage 1 and 2 girls (P < 0.05), but not in girls of later Tanner stages. Mean DHEAS was elevated in Tanner 1 obese girls (P < 0.05), but differences were not statistically significant in the Tanner 2 and 3 girls.
Due to blood volume constraints, fewer data are available for E2, P, and FSH. Nonetheless, no clear differences of E2 or P were seen between obese and normal weight girls in any Tanner stage grouping (Table 1). However, pubertal P concentrations tended to be higher than prepubertal values (P = NS). Mean FSH was lower in Tanner 1 obese girls only (P < 0.05).
Overnight changes in sex steroids in normal weight and obese pre- and early pubertal girls
Twenty-three Tanner stage 1–3 girls had sex steroid measurements in the late evening (2200–2300 h) and subsequent morning (circa 0500–0700 h) (Figure 3). In the group of normal weight girls (n = 17), mean P demonstrated a 2.3-fold overnight increase (P < 0.001), while mean total T showed a 2.4-fold overnight increase (P = 0.001). There was no difference in E2.
Figure 3
Figure 3
Overnight changes in sex steroid concentrations (Tanner 1–3)
Mean P and T in the evening were 2.8- and 5.3-fold higher in the group of obese Tanner 1–3 girls (n = 6) compared to normal weight girls (P < 0.05 for P; P = NS for T). Mean P and T tended to rise overnight in obese girls (1.4-fold and 1.6-fold increase, respectively), but these changes were not statistically significant.
Cortisol values increased overnight in both normal weight girls (1.9 ± 0.4 to 12.0 ± 0.8 μg/dl) and obese girls (3.1 ± 1.0 to 13.8 ± 1.8 μg/dl; P < 0.05 for both comparisons). Cortisol values at corresponding time points were not different between normal weight and obese girls.
In our cohort of girls spanning the range of pubertal development, obesity is associated with marked HA and hyperinsulinemia, and this was especially evident in pre- and early pubertal girls. Total T was elevated in obese peripubertal girls: thus, while reduced SHBG contributes to the elevation of free T in these subjects, T secretion was also increased. Additionally, DHEAS was elevated in prepubertal obese girls, which is consistent with earlier reports (6, 16). These data augment our earlier observations (7) and provide detailed assessment of hormonal values across pubertal maturation. They also emphasize that obesity is commonly associated with HA, with 66–94% of obese Tanner 1–3 girls having an elevated free T.
In keeping with the known association between childhood obesity and both insulin resistance and hyperinsulinemia (17), fasting insulin was elevated in obese peripubertal girls, especially in Tanner 1–3 girls. This finding was supported by elevations in HOMA in obese girls, though it is unclear why both parameters appear disproportionately elevated before and during early puberty. The concomitant elevation of insulin and T in this and other studies (2, 7) suggest a possible mechanism for HA in obese girls. Namely, insulin may act as a co-gonadotropin on the pubertal ovarian theca cell compartment to promote androgen production (1, 18). Insulin also decreases hepatic SHBG production, contributing to elevations in free T (1). Additionally, extensive data supports an important role of hyperinsulinemia in the pathogenesis of adult PCOS, primarily via mechanisms discussed above. However, partial correlation analysis in our earlier report (7) revealed a strong association between obesity and free T even after adjusting for differences in fasting insulin, suggesting that insulin may not be the only mediator of HA in obese girls. We recognize that our assessment of insulin resistance and hyperinsulinemia is limited, as insulin values were not available for all subjects, and a single fasting insulin (or HOMA) is an imprecise marker of hyperinsulinemia. Regardless, available data support a role of insulin in the HA associated with peripubertal obesity.
Our results suggest that early morning LH is relatively low in pre- and early pubertal obese girls, but normalizes as pubertal development progresses. This observation in Tanner 1–3 girls is reminiscent of an inverse relationship between BMI and both mean LH and LH amplitude in adults with PCOS (19, 20). However, the association of obesity with advanced physical exam characteristics of puberty has been well-described (21). Thus, the lower LH in the Tanner 2 obese girls may in part reflect their younger chronological age and relatively immature GnRH-gonadotrope axis compared to their normal weight counterparts. Taken together, the patterns of testosterone, LH, and insulin changes across puberty suggest that insulin plays a particularly important role in obesity-associated HA in pre- and early pubertal girls, with progressive maturation of the hypothalamic-pituitary unit (concomitant with Tanner stage 3) and associated rise in gonadotropins further promoting ovarian T production. Indeed, our earlier partial correlation analysis (7) disclosed a positive correlation between LH and free T after adjusting for differences in age, pubertal stage, BMI, DHEAS, and insulin. We recognize that early morning LH values do not fully characterize LH secretion during puberty, and further study is required to assess LH secretory patterns in obese peripubertal girls. Overall, however, these data suggest that hyperinsulinemia plays a major role in obesity-associated HA in pre- and early puberty, with both LH and insulin contributing to HA as puberty progresses.
Our group of subjects included a number of girls with clinical evidence of hyperandrogenism. We previously argued (7) that systematic exclusion of such girls may inappropriately lessen the apparent relationship between adiposity and androgen excess. Regardless, analysis after exclusion of such girls was not materially different (data not shown) and did not alter interpretation. Any recruitment bias was especially unlikely to have influenced results in the Tanner 1–3 group: very few of these girls had clinical hyperandrogenism, as the manifestations of androgen excess develop slowly.
Previous reports described diurnal variation in T (9, 10) and E2 (811), with peaks occurring between 0600 and 1000 h. Progesterone was not measured in these studies, and our results show that in normal weight girls, mean P concentrations rose 2.3-fold from 2300 h to 0600 h, similar to the increase observed for T. Although P and T appear to increase overnight in obese Tanner 1–3 girls, these differences did not quite reach statistical significance, possibly reflecting the higher evening levels and the limited numbers of obese subjects studied. Overnight changes of E2 were not observed in our study; this may reflect the timing of surveillance, as E2 tends to peak later in the morning (8, 10, 11).
The origin of pubertal P and T secretion—and overnight increases in plasma concentrations—remain unclear: ovarian P secretion may be stimulated by the overnight increases in LH observed in pubertal girls (2224), and adrenal P secretion may also increase under ACTH control. The importance of overnight increases of sex steroids during puberty is also unclear, but may influence LH pulsatility in pubertal girls. For instance, acute E2 infusion mitigates the overnight increase of LH secretion in pubertal girls (23). In addition, whereas normal Tanner 1–3 girls demonstrate nocturnal increases in LH (and by inference GnRH) pulse frequency, age-matched girls with gonadal dysgenesis (i.e., absent ovarian steroid secretion) do not (22). Of parallel interest is that, when expressed in mass or molar terms, P concentrations exceeded those of both T and E2 in normal weight girls, and P is the major regulator or GnRH pulse frequency in adult women (25). Taken together, these findings highlight the importance of delineating the potential regulatory role of P and E2 in directing diurnal changes of GnRH and LH secretion during female puberty.
In adults with PCOS, relative resistance of the GnRH pulse generator to negative feedback by P plays a role in the persistently rapid GnRH pulse frequency, elevated LH concentrations, and relative FSH deficiency characteristic of this syndrome (2628). Some adolescents with hyperandrogenemia (HA) demonstrate a similar feedback defect (29). In adult PCOS, this relative insensitivity is reversed by androgen-receptor blockade (flutamide), suggesting that it is a consequence of hyperandrogenemia per se (30). We have proposed (31) a hypothetical paradigm in which early morning increases in sex steroids (especially P)—either directly by actions on the GnRH pulse generator or via facilitation of higher CNS mechanisms—contribute to the reduction of GnRH and LH pulsatility during the following day. Diurnal slowing of GnRH pulsatility would favor FSH synthesis, secretion, and subsequent follicular development. In girls with HA-induced decreases in hypothalamic sensitivity to feedback inhibition, overnight increases of sex steroids would not slow GnRH pulsatility during the subsequent day. A persistently (24 h) rapid GnRH pulse stimulus would enhance LH and ovarian androgen secretion, and lead to relative FSH deficiency with impaired follicular development. We seek to explore the viability of this paradigm in ongoing and future studies.
In conclusion, peripubertal obesity is associated with hyperinsulinemia and hyperandrogenemia, which is particularly marked in pre- and early pubertal girls. Additionally, progesterone and testosterone concentrations in normal Tanner 1–3 girls increase from 2300 to 0600 h. Elucidation of the origin and consequences of the normal overnight increase in sex steroids and of obesity-associated hyperandrogenemia requires additional study.
Acknowledgments
We gratefully acknowledge Amy B. Bellows, Ph.D., Chandan Chopra, and Quirine Lamberts Okonkwo, M.D., for subject recruitment, study scheduling, and assistance with data management. We also extend our gratitude to Alan D. Rogol, M.D.; William L. Clarke, M.D.; and Milagros G. Huerta, M.D., for referral of obese subjects; to the nurses and staff of the General Clinical Research Centers (University of Virginia, University of California San Diego, University of Michigan, Yale) for implementation of these sampling protocols; and to the Center for Research in Reproduction Ligand Core Laboratory for performance of all assays.
This work was supported by the National Institute of Child Health and Human Development, National Institutes of Health, through Cooperative Agreements U54-HD-28934 (University of Virginia) and U54-HD-12303 (University of California-San Diego) as a part of the Specialized Cooperative Centers Program in Reproduction Research; General Clinical Research Center Grants M01-RR-00847 (University of Virginia), MO1-RR-00827 (University of California-San Diego), MO1-RR00125 (Yale), and M01-RR-00042 (University of Michigan); and grants U54-HD-28934 (J.C.M.); R01-HD-40787 (S. Caprio); K23-HD-044742 (C.R.M.); F32-HD-DK42895 (K.A.P.); and T32-HD-07382 (S.K.B., S. Chhabra, and K.D.H.).
Footnotes
*This is an un-copyedited author manuscript copyrighted by The Endocrine Society. This may not be duplicated or reproduced, other than for personal use or within the rule of “Fair Use of Copyrighted Materials” (section 107, Title 17, U.S. Code) without permission of the copyright owner, The Endocrine Society. From the time of acceptance following peer review, the full text of this manuscript is made freely available by The Endocrine Society at http://www.endojournals.org/. The final copy edited article can be found at http://www.endojournals.org/. The Endocrine Society disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by the National Institutes of Health or other parties. The citation of this article must include the following information: author(s), article title, journal title, year of publication and DOI.
Reprint requests should be addressed to the corresponding author (above).
None of the authors has any potential conflicts of interest to declare.
1. Poretsky L, Cataldo NA, Rosenwaks Z, Giudice LC. The insulin-related ovarian regulatory system in health and disease. Endocr Rev. 1999;20:535–82. [PubMed]
2. Wabitsch M, Hauner H, Heinze E, Bockmann A, Benz R, Mayer H, Teller W. Body fat distribution and steroid hormone concentrations in obese adolescent girls before and after weight reduction. J Clin Endocrinol Metab. 1995;80:3469–75. [PubMed]
3. Dunkel L, Sorva R, Voutilainen R. Low levels of sex hormone--binding globulin in obese children. J Pediatr. 1985;107:95–7. [PubMed]
4. De Simone M, Verrotti A, Iughetti L, Palumbo M, Farello G, Di Cesare E, Bernabei R, Rosato T, Lozzi S, Criscione S. Increased visceral adipose tissue is associated with increased circulating insulin and decreased sex hormone binding globulin levels in massively obese adolescent girls. J Endocrinol Invest. 2001;24:438–44. [PubMed]
5. van Hooff MH, Voorhorst FJ, Kaptein MB, Hirasing RA, Koppenaal C, Schoemaker J. Insulin, androgen, and gonadotropin concentrations, body mass index, and waist to hip ratio in the first years after menarche in girls with regular menstrual cycles, irregular menstrual cycles, or oligomenorrhea. J Clin Endocrinol Metab. 2000;85:1394–400. [PubMed]
6. Reinehr T, de Sousa G, Roth CL, Andler W. Androgens before and after weight loss in obese children. J Clin Endocrinol Metab. 2005;90:5588–95. [PubMed]
7. McCartney CR, Prendergast KA, Chhabra S, Eagleson CA, Yoo R, Chang RJ, Foster CM, Marshall JC. The Association of Obesity and Hyperandrogenemia during the Pubertal Transition in Girls: Obesity as a Potential Factor in the Genesis of Postpubertal Hyperandrogenism. J Clin Endocrinol Metab. 2006;91:1714–22. [PubMed]
8. Goji K. Twenty-four-hour concentration profiles of gonadotropin and estradiol (E2) in prepubertal and early pubertal girls: the diurnal rise of E2 is opposite the nocturnal rise of gonadotropin. J Clin Endocrinol Metab. 1993;77:1629–35. [PubMed]
9. Mitamura R, Yano K, Suzuki N, Ito Y, Makita Y, Okuno A. Diurnal rhythms of luteinizing hormone, follicle-stimulating hormone, testosterone, and estradiol secretion before the onset of female puberty in short children. J Clin Endocrinol Metab. 2000;85:1074–80. [PubMed]
10. Ankarberg C, Norjavaara E. Diurnal rhythm of testosterone secretion before and throughout puberty in healthy girls: correlation with 17 beta-estradiol and dehydroepiandrosterone sulfate. J Clin Endocrinol Metab. 1999;84:975–84. [PubMed]
11. Norjavaara E, Ankarberg C, Albertsson-Wikland K. Diurnal rhythm of 17 beta-estradiol secretion throughout pubertal development in healthy girls: evaluation by a sensitive radioimmunoassay. J Clin Endocrinol Metab. 1996;81:4095–102. [PubMed]
12. Kuczmarski RJ, Ogden CL, Grummer-Strawn LM, Flegal KM, Guo S, Wei R, Mei Z, Curtin LR, Roche AF, Johnson CL. 2000 CDC growth charts: United States. Adv Data. :1–27. [PubMed]
13. Marshall WA, Tanner JM. Variations in pattern of pubertal changes in girls. Arch Dis Child. 1969;44:291–303. [PMC free article] [PubMed]
14. Vermeulen A, Verdonck L, Kaufman JM. A critical evaluation of simple methods for the estimation of free testosterone in serum. J Clin Endocrinol Metab. 1999;84:3666–72. [PubMed]
15. Legro RS, Castracane VD, Kauffman RP. Detecting insulin resistance in polycystic ovary syndrome: purposes and pitfalls. Obstet Gynecol Surv. 2004;59:141–54. [PubMed]
16. l’Allemand D, Schmidt S, Rousson V, Brabant G, Gasser T, Gruters A. Associations between body mass, leptin, IGF-I and circulating adrenal androgens in children with obesity and premature adrenarche. Eur J Endocrinol. 2002;146:537–43. [PubMed]
17. Strauss RS. Childhood obesity. Pediatr Clin North Am. 2002;49:175–201. [PubMed]
18. Barbieri RL, Makris A, Randall RW, Daniels G, Kistner RW, Ryan KJ. Insulin stimulates androgen accumulation in incubations of ovarian stroma obtained from women with hyperandrogenism. J Clin Endocrinol Metab. 1986;62:904–10. [PubMed]
19. Arroyo A, Laughlin GA, Morales AJ, Yen SS. Inappropriate gonadotropin secretion in polycystic ovary syndrome: influence of adiposity. J Clin Endocrinol Metab. 1997;82:3728–33. [PubMed]
20. Taylor AE, McCourt B, Martin KA, Anderson EJ, Adams JM, Schoenfeld D, Hall JE. Determinants of abnormal gonadotropin secretion in clinically defined women with polycystic ovary syndrome. J Clin Endocrinol Metab. 1997;82:2248–56. [PubMed]
21. Wang Y. Is obesity associated with early sexual maturation? A comparison of the association in American boys versus girls. Pediatrics. 2002;110:903–10. [PubMed]
22. Cemeroglu AP, Foster CM, Warner R, Kletter GB, Marshall JC, Kelch RP. Comparison of the neuroendocrine control of pubertal maturation in girls and boys with spontaneous puberty and in hypogonadal girls. J Clin Endocrinol Metab. 1996;81:4352–7. [PubMed]
23. Cemeroglu AP, Kletter GB, Guo W, Brown MB, Kelch RP, Marshall JC, Padmanabhan V, Foster CM. In pubertal girls, naloxone fails to reverse the suppression of luteinizing hormone secretion by estradiol. J Clin Endocrinol Metab. 1998;83:3501–6. [PubMed]
24. Apter D, Butzow TL, Laughlin GA, Yen SS. Gonadotropin-releasing hormone pulse generator activity during pubertal transition in girls: pulsatile and diurnal patterns of circulating gonadotropins. J Clin Endocrinol Metab. 1993;76:940–9. [PubMed]
25. Soules MR, Steiner RA, Clifton DK, Cohen NL, Aksel S, Bremner WJ. Progesterone modulation of pulsatile luteinizing hormone secretion in normal women. J Clin Endocrinol Metab. 1984;58:378–83. [PubMed]
26. Marshall JC, Eagleson CA. Neuroendocrine aspects of polycystic ovary syndrome. Endocrinol Metab Clin North Am. 1999;28:295–324. [PubMed]
27. Pastor CL, Griffin-Korf ML, Aloi JA, Evans WS, Marshall JC. Polycystic ovary syndrome: evidence for reduced sensitivity of the gonadotropin-releasing hormone pulse generator to inhibition by estradiol and progesterone. J Clin Endocrinol Metab. 1998;83:582–90. [PubMed]
28. Daniels TL, Berga SL. Resistance of gonadotropin releasing hormone drive to sex steroid-induced suppression in hyperandrogenic anovulation. J Clin Endocrinol Metab. 1997;82:4179–83. [PubMed]
29. Chhabra S, McCartney CR, Yoo RY, Eagleson CA, Chang RJ, Marshall JC. Progesterone inhibition of the hypothalamic gonadotropin-releasing hormone pulse generator: evidence for varied effects in hyperandrogenemic adolescent girls. J Clin Endocrinol Metab. 2005;90:2810–5. [PubMed]
30. Eagleson CA, Gingrich MB, Pastor CL, Arora TK, Burt CM, Evans WS, Marshall JC. Polycystic ovarian syndrome: evidence that flutamide restores sensitivity of the gonadotropin-releasing hormone pulse generator to inhibition by estradiol and progesterone. J Clin Endocrinol Metab. 2000;85:4047–52. [PubMed]
31. Blank SK, McCartney CR, Marshall JC. The origins and sequelae of abnormal neuroendocrine function in polycystic ovary syndrome. Hum Reprod Update. 2006;12:351–61. [PubMed]