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J Exp Med. 1993 January 1; 177(1): 119–125.
PMCID: PMC2190864

Antigen recognition properties of mutant V beta 3+ T cell receptors are consistent with an immunoglobulin-like structure for the receptor


We examined the effect of mutations in the V beta portion of a pigeon cytochrome c (cyto c)-specific V beta 3+/V alpha 11+ T cell receptor on its ability to recognize cyto c/IEk and various superantigens. The results were consistent with an immunoglobulin-like structure for the receptor V beta domain and with separate interaction sites on V beta for conventional antigen and superantigens. An amino acid predicted to lie in CDR1 was critical for cyto c/IEk but not superantigen recognition, while several amino acids predicted to lie in the hypervariable region 4 loop were critical for superantigen but not cyto c/IEk recognition.

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Selected References

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  • Schiffer M, Wu TT, Kabat EA. Subgroups of variable region genes of beta chains of T-cell receptors for antigen. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4461–4463. [PubMed]
  • Chothia C, Boswell DR, Lesk AM. The outline structure of the T-cell alpha beta receptor. EMBO J. 1988 Dec 1;7(12):3745–3755. [PubMed]
  • Davis MM, Bjorkman PJ. T-cell antigen receptor genes and T-cell recognition. Nature. 1988 Aug 4;334(6181):395–402. [PubMed]
  • Chien YH, Gascoigne NR, Kavaler J, Lee NE, Davis MM. Somatic recombination in a murine T-cell receptor gene. Nature. 1984 May 24;309(5966):322–326. [PubMed]
  • Siu G, Clark SP, Yoshikai Y, Malissen M, Yanagi Y, Strauss E, Mak TW, Hood L. The human T cell antigen receptor is encoded by variable, diversity, and joining gene segments that rearrange to generate a complete V gene. Cell. 1984 Jun;37(2):393–401. [PubMed]
  • Chou HS, Nelson CA, Godambe SA, Chaplin DD, Loh DY. Germline organization of the murine T cell receptor beta-chain genes. Science. 1987 Oct 23;238(4826):545–548. [PubMed]
  • Winoto A, Mjolsness S, Hood L. Genomic organization of the genes encoding mouse T-cell receptor alpha-chain. Nature. 316(6031):832–836. [PubMed]
  • Padlan EA, Silverton EW, Sheriff S, Cohen GH, Smith-Gill SJ, Davies DR. Structure of an antibody-antigen complex: crystal structure of the HyHEL-10 Fab-lysozyme complex. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5938–5942. [PubMed]
  • Sheriff S, Silverton EW, Padlan EA, Cohen GH, Smith-Gill SJ, Finzel BC, Davies DR. Three-dimensional structure of an antibody-antigen complex. Proc Natl Acad Sci U S A. 1987 Nov;84(22):8075–8079. [PubMed]
  • Marquart M, Deisenhofer J, Huber R, Palm W. Crystallographic refinement and atomic models of the intact immunoglobulin molecule Kol and its antigen-binding fragment at 3.0 A and 1.0 A resolution. J Mol Biol. 1980 Aug 25;141(4):369–391. [PubMed]
  • Fischmann TO, Bentley GA, Bhat TN, Boulot G, Mariuzza RA, Phillips SE, Tello D, Poljak RJ. Crystallographic refinement of the three-dimensional structure of the FabD1.3-lysozyme complex at 2.5-A resolution. J Biol Chem. 1991 Jul 15;266(20):12915–12920. [PubMed]
  • Lascombe MB, Alzari PM, Boulot G, Saludjian P, Tougard P, Berek C, Haba S, Rosen EM, Nisonoff A, Poljak RJ. Three-dimensional structure of Fab R19.9, a monoclonal murine antibody specific for the p-azobenzenearsonate group. Proc Natl Acad Sci U S A. 1989 Jan;86(2):607–611. [PubMed]
  • Winoto A, Urban JL, Lan NC, Goverman J, Hood L, Hansburg D. Predominant use of a V alpha gene segment in mouse T-cell receptors for cytochrome c. Nature. 1986 Dec 18;324(6098):679–682. [PubMed]
  • Engel I, Hedrick SM. Site-directed mutations in the VDJ junctional region of a T cell receptor beta chain cause changes in antigenic peptide recognition. Cell. 1988 Aug 12;54(4):473–484. [PubMed]
  • Jorgensen JL, Esser U, Fazekas de St Groth B, Reay PA, Davis MM. Mapping T-cell receptor-peptide contacts by variant peptide immunization of single-chain transgenics. Nature. 1992 Jan 16;355(6357):224–230. [PubMed]
  • Nalefski EA, Wong JG, Rao A. Amino acid substitutions in the first complementarity-determining region of a murine T-cell receptor alpha chain affect antigen-major histocompatibility complex recognition. J Biol Chem. 1990 May 25;265(15):8842–8846. [PubMed]
  • Pullen AM, Wade T, Marrack P, Kappler JW. Identification of the region of T cell receptor beta chain that interacts with the self-superantigen MIs-1a. Cell. 1990 Jun 29;61(7):1365–1374. [PubMed]
  • Pullen AM, Bill J, Kubo RT, Marrack P, Kappler JW. Analysis of the interaction site for the self superantigen Mls-1a on T cell receptor V beta. J Exp Med. 1991 May 1;173(5):1183–1192. [PMC free article] [PubMed]
  • Choi YW, Kotzin B, Herron L, Callahan J, Marrack P, Kappler J. Interaction of Staphylococcus aureus toxin "superantigens" with human T cells. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8941–8945. [PubMed]
  • Cazenave PA, Marche PN, Jouvin-Marche E, Voegtlé D, Bonhomme F, Bandeira A, Coutinho A. V beta 17 gene polymorphism in wild-derived mouse strains: two amino acid substitutions in the V beta 17 region greatly alter T cell receptor specificity. Cell. 1990 Nov 16;63(4):717–728. [PubMed]
  • Kappler JW, Skidmore B, White J, Marrack P. Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition. J Exp Med. 1981 May 1;153(5):1198–1214. [PMC free article] [PubMed]
  • Pullen AM, Marrack P, Kappler JW. The T-cell repertoire is heavily influenced by tolerance to polymorphic self-antigens. Nature. 1988 Oct 27;335(6193):796–801. [PubMed]
  • Jameson SC, Nakajima PB, Brooks JL, Heath W, Kanagawa O, Gascoigne NR. The T cell receptor V alpha 11 gene family. Analysis of allelic sequence polymorphism and demonstration of J alpha region-dependent recognition by allele-specific antibodies. J Immunol. 1991 Nov 1;147(9):3185–3193. [PubMed]
  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. [PubMed]
  • Burgert HG, White J, Weltzien HU, Marrack P, Kappler JW. Reactivity of V beta 17a+ CD8+ T cell hybrids. Analysis using a new CD8+ T cell fusion partner. J Exp Med. 1989 Dec 1;170(6):1887–1904. [PMC free article] [PubMed]
  • Kappler JW, Wade T, White J, Kushnir E, Blackman M, Bill J, Roehm N, Marrack P. A T cell receptor V beta segment that imparts reactivity to a class II major histocompatibility complex product. Cell. 1987 Apr 24;49(2):263–271. [PubMed]
  • Ronchese F, Schwartz RH, Germain RN. Functionally distinct subsites on a class II major histocompatibility complex molecule. Nature. 1987 Sep 17;329(6136):254–256. [PubMed]
  • Griffith IJ, Nabavi N, Ghogawala Z, Chase CG, Rodriguez M, McKean DJ, Glimcher LH. Structural mutation affecting intracellular transport and cell surface expression of murine class II molecules. J Exp Med. 1988 Feb 1;167(2):541–555. [PMC free article] [PubMed]
  • Marrack P, Kappler J. T cells can distinguish between allogeneic major histocompatibility complex products on different cell types. Nature. 1988 Apr 28;332(6167):840–843. [PubMed]
  • Arnold LW, Grdina TA, Whitmore AC, Haughton G. Ig isotype switching in B lymphocytes. Isolation and characterization of clonal variants of the murine Ly-1+ B cell lymphoma, CH12, expressing isotypes other than IgM. J Immunol. 1988 Jun 15;140(12):4355–4363. [PubMed]
  • King LB, Lund FE, White DA, Sharma S, Corley RB. Molecular events in B lymphocyte differentiation. Inducible expression of the endogenous mouse mammary tumor proviral gene, Mtv-9. J Immunol. 1990 Apr 15;144(8):3218–3227. [PubMed]
  • Woodland DL, Happ MP, Gollob KJ, Palmer E. An endogenous retrovirus mediating deletion of alpha beta T cells? Nature. 1991 Feb 7;349(6309):529–530. [PubMed]
  • Woodland DL, Lund FE, Happ MP, Blackman MA, Palmer E, Corley RB. Endogenous superantigen expression is controlled by mouse mammary tumor proviral loci. J Exp Med. 1991 Nov 1;174(5):1255–1258. [PMC free article] [PubMed]
  • Nicolas JF, Wegmann D, Lebrun P, Kaiserlian D, Tovey J, Glasebrook AL. Relationship of B cell Fc receptors to T cell recognition of Mls antigen. Eur J Immunol. 1987 Nov;17(11):1561–1565. [PubMed]
  • Kappler JW, Herman A, Clements J, Marrack P. Mutations defining functional regions of the superantigen staphylococcal enterotoxin B. J Exp Med. 1992 Feb 1;175(2):387–396. [PMC free article] [PubMed]
  • Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 Dec 16;65(1-2):55–63. [PubMed]
  • Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol. 1989 Apr 15;142(8):2736–2742. [PubMed]
  • Chien YH, Gascoigne NR, Kavaler J, Lee NE, Davis MM. Somatic recombination in a murine T-cell receptor gene. Nature. 1984 May 24;309(5966):322–326. [PubMed]
  • Gahm SJ, Fowlkes BJ, Jameson SC, Gascoigne NR, Cotterman MM, Kanagawa O, Schwartz RH, Matis LA. Profound alteration in an alpha beta T-cell antigen receptor repertoire due to polymorphism in the first complementarity-determining region of the beta chain. Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10267–10271. [PubMed]
  • Fink PJ, Matis LA, McElligott DL, Bookman M, Hedrick SM. Correlations between T-cell specificity and the structure of the antigen receptor. Nature. 1986 May 15;321(6067):219–226. [PubMed]
  • Callahan JE, Herman A, Kappler JW, Marrack P. Stimulation of B10.BR T cells with superantigenic staphylococcal toxins. J Immunol. 1990 Apr 1;144(7):2473–2479. [PubMed]
  • Herman A, Kappler JW, Marrack P, Pullen AM. Superantigens: mechanism of T-cell stimulation and role in immune responses. Annu Rev Immunol. 1991;9:745–772. [PubMed]
  • Sorger SB, Hedrick SM, Fink PJ, Bookman MA, Matis LA. Generation of diversity in T cell receptor repertoire specific for pigeon cytochrome c. J Exp Med. 1987 Feb 1;165(2):279–301. [PMC free article] [PubMed]
  • Fry AM, Matis LA. Self-tolerance alters T-cell receptor expression in an antigen-specific MHC restricted immune response. Nature. 1988 Oct 27;335(6193):830–832. [PubMed]
  • Fry AM, Cotterman MM, Matis LA. The influence of self-MHC and non-MHC antigens on the selection of an antigen-specific T cell receptor repertoire. J Immunol. 1989 Oct 15;143(8):2723–2729. [PubMed]
  • McIntyre BW, Allison JP. Biosynthesis and processing of murine T-cell antigen receptor. Cell. 1984 Oct;38(3):659–665. [PubMed]
  • Kaye J, Janeway CA., Jr The alpha- and beta-subunits of a murine T cell antigen/Ia receptor have a molecular weight of 31,000 in the absence of N-linked glycosylation. J Immunol. 1984 Nov;133(5):2291–2293. [PubMed]
  • Fleischer B, Schrezenmeier H. T cell stimulation by staphylococcal enterotoxins. Clonally variable response and requirement for major histocompatibility complex class II molecules on accessory or target cells. J Exp Med. 1988 May 1;167(5):1697–1707. [PMC free article] [PubMed]
  • Fischer H, Dohlsten M, Lindvall M, Sjögren HO, Carlsson R. Binding of staphylococcal enterotoxin A to HLA-DR on B cell lines. J Immunol. 1989 May 1;142(9):3151–3157. [PubMed]
  • Herman A, Croteau G, Sekaly RP, Kappler J, Marrack P. HLA-DR alleles differ in their ability to present staphylococcal enterotoxins to T cells. J Exp Med. 1990 Sep 1;172(3):709–717. [PMC free article] [PubMed]
  • Dellabona P, Peccoud J, Kappler J, Marrack P, Benoist C, Mathis D. Superantigens interact with MHC class II molecules outside of the antigen groove. Cell. 1990 Sep 21;62(6):1115–1121. [PubMed]
  • Jorgensen JL, Reay PA, Ehrich EW, Davis MM. Molecular components of T-cell recognition. Annu Rev Immunol. 1992;10:835–873. [PubMed]

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